Title:
METHOD FOR ANALYZING RNA
Document Type and Number:
WIPO Patent Application WO/2011/074592
Kind Code:
A1
Abstract:
A method for analyzing RNA that is extracted from a tissue or cell that has been fixed with a fixing solution, which comprises a step for determining whether or not said RNA satisfies the formula: B/A≤1 [wherein A represents the ratio (%) by weight, in electrophoresis, of RNA having 1000-4000 nucleotides to the total weight of said RNA; B represents the ratio (%) by weight, in electrophoresis, of RNA having more than 4000 nucleotides to the total weight of said RNA]. According to this analysis method, the expression data or the occurrence or non-occurrence of the expression of a gene in the tissue or cell, which has been fixed with the fixing solution, are analyzed.
Inventors:
KURODA TOSHIHIKO (JP)
NOMURA OSAMU (JP)
NOBUMASA HITOSHI (JP)
NOMURA OSAMU (JP)
NOBUMASA HITOSHI (JP)
Application Number:
PCT/JP2010/072518
Publication Date:
June 23, 2011
Filing Date:
December 15, 2010
Export Citation:
Assignee:
TORAY INDUSTRIES (JP)
KURODA TOSHIHIKO (JP)
NOMURA OSAMU (JP)
NOBUMASA HITOSHI (JP)
KURODA TOSHIHIKO (JP)
NOMURA OSAMU (JP)
NOBUMASA HITOSHI (JP)
International Classes:
C12Q1/68; G01N27/447; G16B25/00; G06F17/30
Domestic Patent References:
| WO2004090780A2 | 2004-10-21 |
Other References:
YU SHIMADA ET AL.: "Paraffin Rinsho Kentai o Riyo shita mRNA to miRNA Kaiseki Yogo ya Yakuzai Kanjusei Yosoku Marker Idenshi Tansaku no Kanosei", JAPANESE JOURNAL OF CANCER CLINICS, vol. 55, no. 8, 15 December 2009 (2009-12-15), pages 56 - 57
IMBEAUD, SANDRINE ET AL.: "Towards standardization of RNA quality assessment using user-independent classifiers of microcapillary electrophoresis traces.", NUCLEIC ACIDS RESEARCH, vol. 33, no. 6, 2005, pages 1 - 12, XP055072089
RIBEIRO-SILVA, ALFREDO ET AL.: "RNA extraction from ten year old formalin-fixed paraffin- embedded breast cancer samples: a comparison of column purification and magnetic bead-based technologies.", BMC MOLECULAR BIOLOGY, vol. 8, 2007, pages 1 - 10, XP021033423
MASUDA NORIKAZU ET AL.: "Analysis of chemical modification of RNA from formalin-fixed samples and optimization of molecular biology applications for such samples.", NUCLEIC ACIDS RESEARCH, vol. 27, no. 22, 1999, pages 4436 - 4443, XP002320835
TATSUYUKI GONO ET AL.: "mRNA expression analysis extracted from formalin-fixed paraffin-embedded samples", BULLETIN OF SCHOOL OF HEALTH SCIENCES TOHOKU UNIVERSITY, vol. 18, no. 2, 31 July 2009 (2009-07-31), pages 101 - 110, XP008163859
JARZAB, M. ET AL.: "Optimization of the method of RNA isolation from paraffin blocks to assess gene expression in breast cancer.", POLISH JOURNAL OF PATHOLOGY, vol. 59, no. 2, 2008, pages 85 - 91, XP055072092
MRAZ, M. ET AL.: "MicroRNA isolation and stability in stored RNA samples.", BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, vol. 390, no. 1, 4 December 2009 (2009-12-04), pages 1 - 4, XP026696977
NAGINO KUNIHISA ET AL.: "Ultrasensitive DNA chip: gene expression profile analysis without RNA amplification.", THE JOURNAL OF BIOCHEMISTRY, vol. 139, no. 4, 2006, pages 697 - 703, XP055072093
SATO FUMIAKI ET AL.: "Intra-platform repeatability and inter-platform comparability of microRNA microarray technology.", PLOS ONE, vol. 4, no. 5, May 2009 (2009-05-01), pages 1 - 12, XP055072094
See also references of EP 2514834A4
IMBEAUD, SANDRINE ET AL.: "Towards standardization of RNA quality assessment using user-independent classifiers of microcapillary electrophoresis traces.", NUCLEIC ACIDS RESEARCH, vol. 33, no. 6, 2005, pages 1 - 12, XP055072089
RIBEIRO-SILVA, ALFREDO ET AL.: "RNA extraction from ten year old formalin-fixed paraffin- embedded breast cancer samples: a comparison of column purification and magnetic bead-based technologies.", BMC MOLECULAR BIOLOGY, vol. 8, 2007, pages 1 - 10, XP021033423
MASUDA NORIKAZU ET AL.: "Analysis of chemical modification of RNA from formalin-fixed samples and optimization of molecular biology applications for such samples.", NUCLEIC ACIDS RESEARCH, vol. 27, no. 22, 1999, pages 4436 - 4443, XP002320835
TATSUYUKI GONO ET AL.: "mRNA expression analysis extracted from formalin-fixed paraffin-embedded samples", BULLETIN OF SCHOOL OF HEALTH SCIENCES TOHOKU UNIVERSITY, vol. 18, no. 2, 31 July 2009 (2009-07-31), pages 101 - 110, XP008163859
JARZAB, M. ET AL.: "Optimization of the method of RNA isolation from paraffin blocks to assess gene expression in breast cancer.", POLISH JOURNAL OF PATHOLOGY, vol. 59, no. 2, 2008, pages 85 - 91, XP055072092
MRAZ, M. ET AL.: "MicroRNA isolation and stability in stored RNA samples.", BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, vol. 390, no. 1, 4 December 2009 (2009-12-04), pages 1 - 4, XP026696977
NAGINO KUNIHISA ET AL.: "Ultrasensitive DNA chip: gene expression profile analysis without RNA amplification.", THE JOURNAL OF BIOCHEMISTRY, vol. 139, no. 4, 2006, pages 697 - 703, XP055072093
SATO FUMIAKI ET AL.: "Intra-platform repeatability and inter-platform comparability of microRNA microarray technology.", PLOS ONE, vol. 4, no. 5, May 2009 (2009-05-01), pages 1 - 12, XP055072094
See also references of EP 2514834A4
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