Technical Field

[0001] The present invention relates to a method of providing an agent to cells withi a target region in a subject. The method has particular application in providing enhanced trans ectioTi of one or more cells within a limited target region within a subject, such as a limited target region within a cochlea.

Background Art

[0002] Electroporation is a well-established methodology for delivery of a variety of molecules into cells, including drugs, proteins and nucleic acids. The latter is highlighted by the electroporati on-based delivery of DNA into cells to drive recombinant gene expression. The underlying principle is that an electric field generated by a high voltage pulse between two electrodes causes a transient .dielectric breakdown of the plasma membrane of cells within the high intensity electric field, enabling the negatively-charged DNA to enter the cells. However, the process by which the DNA/cell membrane interface responds to the electric field to enable the DNA to enter the cell is not well understood.

[0003] The most common use for electroporation-based gene deliver is for molecular biology research, where simple plate electrodes within cuvettes enable routine transformation, of competent ceils o the bench. Electroporation-based gene delivery has subsequently been extended to in situ, ex vivo, and in vivo applications with development of specialised electroporation systems. These electroporation systems include a variety of electrode designs and voltage pulse shaping as part of optimised electroporation parameters, along with custom electroporation solutions and electrodes, with pulse intensity, pulse duration and repetition frequency being key parameters. These systems have proved effective in facilitating research in a range of tissues, including developmental neurobiology applications.

[0004| However, electroporation is still considered problematic for therapeutic gene delivery due to low transformation efficiency, difficulty in placement of electrodes into tissues and the typically high voltages that need to be applied to destabilise the ceil membrane. This can detrimentally affect the target organs and tissues, and may also impact on DNA stability. [Θ005] Despite the breadth of uptake of electroporation both in vitro and in vivo, current technology h s been limited to the application of electric fields utilising conventional electrode configurations (typically plates and needles separated by tissue).

[00061 To date, no reports exist of electroporatioti-based gene delivery into the mature bony cochlea, where paddle electrodes or penetrating needle electrodes are untenable. WO 20 J 1/006204 reports use of a single electrode inserted into the base of the cochlea to deliver an electric field and an external current return electrode, with applications of up to 100 V and up to 20 x 50 ms pulses immediately after injecting plasmid DMA into the cochlea fluid compartments. Tins achieved a number of transfected cells expressing a fluorescence reporter protein, but transformation efficiency was iiraited, as was specificity for the region targeted for transformation,

[0007] There is therefore a need for improved techniques which allow for more efficient and/or more targeted transfection of cells in vivo.

Summary

[0008] The present studies hav surprisingly found that generation of high field strengths within millimetre to sub-millimetre dimensional space (close-field) allows for highly efficient and targeted transformation of mammalian cells. The efficacy of this new mode of electroporation was greater than that of conventional 'open-field' electroporation - where electrodes are separated by tissue, with lower required voltages for transfection of the cells, and is hereinafter termed Close-Field Electroporation ('CFE'),

[0009] Thus, according to a first embodiment of the invention, there is provided a method of transfecting one or more cells within a target region with an agent by electroporation, said method comprising exposing said one or more cells to said agent and to a close electric field created between an anode or anode array and a cathode or cathode array, in said target region for sufficient time to allow at. least some of said agent to enter said one or more cells . The electrodes may be contiguous to an. array structure of at least 2 electrode elements, but electrode or electrode elements may be separated by a distance of less than 5mm. According to an embodiment subjects that may be treated by methods according to the present invention comprise human and non-human animals. The close electric field may be generated by the anode or anode array and the cathode or cathode array being separated by between about ίθμηα and about 5mm, more typically between about 50,um and about 1 mm, and more typically between about 50μιη and about 0.5mm. [OOlOj The methods of the present invention allow for use of significantly lower voltages while maintaining highly efficient transformation of cells. According to an embodiment, the voltage applied between the anode or anode array and the cathode or cathode array is about IV to about 40V, more typically from about I V to about 30V, and even more typically from about 4V to abou 20V.

[0011] The methods of the present invention also allow for significantly lower total charge delivery while maintaining highl efficient transformation of cells, significantly reducing the risk of collateral damage to cells in the region of cells being treated. According to an embodiment die total electiic charge delivered during said electroporation is less than 4 Coulombs, more typically less than 0.4 Coulombs, more typically less than 0.1 Coulombs, and more typically less than 0.01 Coulombs. Ignoring the roughness factor (the fine 3- dimensional structure of platinum electrodes), the effective charge density on platinum electrodes may be between about lmC/cm ² and about lC/cnr ⁴ , such as between about 25 mC cm ^* and about 250 mC/cm" .

[0012] Methods according to the present invention may be carried out using any suitable arrangement of anode(s) and cathade(s) provided they provide a close electric field, thus providin a focussed high electric field in the region of the target cells. Such arrangements may comprise separately inserted or applied electrodes, or anodes and cathodes provided on the same probe. According to an embodiment, the anode or anode array and the cathode or cathode array are provided on a single probe to be introduced into said region. An example of an electrode configuration contemplated by the present invention is embodied by a cochlear implant electrode array. Electrodes of a cochlear implant electrode array may be in a linear configuration and configured to have distal electrode(s) as an anode or cathode array and proximal electrode(s) as an array of the opposite polarity (viz. cathode or anode array). Alternatively, the electrodes may be in a planar configuration, with anode(s) and cathode(s) interspersed or configured substantially or completely on opposite sides of the plane. An anode or cathode array may each, independently, comprise any number of electrodes. According to an embodiment, an anode or cathode array may each, independently, comprise from 2 to 8 electrodes and, more typically, each of the arrays comprises 4 electrodes.

[0013] Methods according to the present invention may be used to transfeet cells within a target region with any desirable agent amenable to electrophoretic transfer into cells. Such agents may include, for example, nucleic acids and analogues thereof, peptides and proteins and analogues thereof as well as other small or large molecules. According to an embodiment, the agent is a nucleic acid. In another embodiment the agent may comprise or be a fluorescent or other labelling moiety or may comprise or be a photoactivatable moiety., such as may be employed in cancer treatment.

18014] Methods according to the present invention ma be for producing, enhancing or suppressing an activity within said region and may be, for example, for at least partially restoring physiological function within said target region, one or more regions proximal to said target region, or both within said target region and one or more regions proximal to said target region. In another embodiment, methods according to the present invention may be for regulation of cell function or for localized cancer treatment.

[0015] According to specific embodiments, methods according to the present invention may be for transfecting cells within a targeted region of the cochlea of a subject. In such embodiments, the agent may comprise or consist of a nucleic acid molecule. n such embodiments, the close electric field may be provided by a cochlear implant electrode array and, in a particular embodiment, a distal half of the operational electrodes may be configured as an anode array or cathode array and a proximal half of the operational electrodes may be configured as an opposite electrode array (i.e. cathode or anode). In another embodiment the electrodes within a cochlear implant array may be divided into blocks of anode or cathodes, or combinations thereof. The nucleic acid molecule may encode a neurotrophic factor for peripheral sensory neurons, and in particular embodiments the nucleic acid molecule encodes a neurotrophic factor for spiral ganglion cells. In particular embodiments the nucleic acid molecule encodes a transcription factor which promotes the expression of a neurotrophic factor. In particular embodiments the nucleic acid molecule encodes one or more therapeutic molecules for spiral ganglion cells.

[0016] In particular embodiments the ceils in the target region in the subject are cells of the scala tympani or scala vestibuli, such as mesenchymal cells.

[0 17| According to anothe embodiment of the invention, there is provided an electroporation electrode assembly comprising an anode or anode array and a cathode or cathode array for creating a close electric field in a target region, for transfecting one or more cells in a target region by a method of the invention as described above.

Brief Description of the Drawings

[0018] Certain embodiments of the present invention will now be described, by way of example only, with, reference to the accompanying drawings.

[DO 19] Figure 1 shows an exemplary gene therapy construct for use in methods according to the present invention, and "alternating" and "tandem" electrode configuration in a cochlear implant 8-electrode mieroarray. a) BDNF-GFP plasmid: cytomegalovirus promoter (CM Vp) upstream of the FLAG-tagged brain-derived neurotrophic factor (BDNF) gene cDNA. The BDNF and green fluorescent protein fused to a nuclear localization site (GFPnls) coding region are transcribed simultaneously, but translated as separate proteins via an internal ribosome entry site (IRES), pA, SV40n polyadenylation site; Qri , origin of replication; aph, kanamycin selection, b) Alternating and tandem electrode close-field eiectroporation mieroarray configurations, c) Exposed cochlea with the eight electrode cochlear implant mieroarray overlaid, d) Cochlear" implant inserted into scala tympani via the round window.

[0020] Figure 2 shows electrical modelling of the electrical field in close-field eiectroporation ("CFE"). A, shows a model of a linear mieroarray with 8 flat electrodes with a surface area of 60pm ² each in either the 'tandem', or 'alternating' electrode configuration, overall length 1mm. The model predicts an even, high field strength at the anodes, while i the 'alternating' electrode layout, there is a bias in field strength to the distal end. This maps well to pilot data f om transfections of ΠΕΚ293 cell sheets (see Fig. 6) utilising a cochlear implant mieroarray. B. A model of electric fields arising from CFE utilizing central electrodes in a hexagonal array (7?0pm electrode pitch), with a single retur electrode; insert shows fabrication layout of a planar mieroarray.

[0021] Figure 3 shows a number of possible cochlear implant mieroarray configurations for use in methods according to the present invention.

[0022] Figure 4 provides a bar chart for numbers of close field eiectroporation transfeeted cells for treatment of confluent HEK293 cells on Poly-D-lysine coated coverslips by methods according to the present invention using a range of electrode configurations and electrosttm ulation protocols.

[00231 Figure 5 Shows fluorescence imaging of Green Fluorescence Protein (GFP) labeling in the nuclei of HEK293 cells in monolayers. Cells were imaged 48 hours after close-field eiectroporation with a naked cDNA construct which drove the GFP expression under a CMV promoter. Tandem & alternating configurations of the mieroarray are as shown: A, and B. are images with the mieroarray superimposed on top of the HE 293 cell monolayer to indicate the scale; 40V, lOpulses, 50ms square wave close-field eiectroporation. C and D. 40V, 5 pulses, 50ms. E and F. 40V, 5 pulses, 20ms. The arrowheads indicate regions of transfeeted cells. Dark lines in Panels C-F indicate mieroarray placement. [Θ024] Figure 6 provides a fluorescence photomicrograph, illustrating HEK293 ceils teansfected using CFE methodology according to the present application employing a "tandem" electrode configuration (as illustrated in Fig. 6B) with the microarray overlaid on the fixed HEK293 cell sheet. Transfected cells are evident as GFP positive. Cells were treated with 10 40 V poises at 50ms per pulse.

[0025] Figure 7 shows results for ex vivo CFE of guinea pig cochleae using cochlear implant electrode micraarrays in alternating or tandem electrode configurations: a), b) ^■ contocal projected images of the distribution of transfomied mesenchymal cells (nuclear localized GFP fluorescence) lining the perilymphatic compartments (scala tympani (ST) and scala vesttbuH (SV)) of the cochlea (white arrowheads). Note the basal location of the transformants, which demonstrates site specificity of gene delivery a) 20V - 5 pulses tandem configuration; b) 40V - 20 pulses alternating configuration, c) Control: BDNF-GFP plasmid loading with cochlear implant, without CFE. d) BDNF production b}' the transformed mesenchymal, cells was confirmed by anti-FLAG immunofluorescence co-incident with the GFP nuclear labelling, e) Effect of voltage on electroporation efficiency (GFP +ve cells ) for the two different close-field electroporation modalities. Lines of best fit: tandem array, y - a+b*x, where a = 25.8, b = 1.71, R ^* = 0.39; alternating array: y= a+b*x, where a = 6.2, b - 0.79, R" = 0.71: mean ± s.e.m.; multiple data points at each voltage level reflect variation in number of pulses (see Table I). ** / ^>= 0.005; ANQVA, Holm-sidak pairwise comparison, f) Effect of charge on mesenchymal cell transformation . Tandem configurati on;

y - a x exp [-0.5(1η(χ,¾)/¾) ² ], where a = 3.0.12, b = 1.354, x ₀ = 0.072, R ² - 0.787

demonstrating a distinct optimum corresponding to 20 V 5 - 10 pulses. Alternating configuration: y- a+b*x, where a - 9 J , b - 174.4, R ³ = 0.948. * P = 0.03.9; Mann-Whitney Rank Sum test.

[00261 Figure 8 illustrates results fo ex. vivo CFE of a BDNF-GFP expression cassette using a) tandem and b) alternating microarray configurations. Nuclear GFP fluorescence is evident in mesenchymal cells lining the perilymphatic compartment in the basal turn region of the guinea pig cochlea - SV, scala vestibuH; ST, scala tympani; SL, spiral limbus, Confocal 3D reconstructions.

[0027] Figure 9 illustrates localisation of CFE BDNF-GFP transformants in the guinea pig cochlea ex vivo and in vivo, a) Cryosection (50 μτη) identifying the transformed cells (GFP +ve; white dots) as mesencyhymat cells lining the perilymphatic compartments (arrowheads). ST, scala tympani; SV, scala vestibuii; SM,. scala media; RM, Reissner's membrane. CFE: tandem configuration 20V - 5 pulses; in vivo at day 4. b) Fluorescence imaging of transformed mesenchymal, cells in scala vestibuli (SV) - GFP nuclear labelling (white). CFE: alternating configuration 40V - 20pulses; ex vivo, 3 days organotypic culture. OSL, osseous spiral lamina; SL, spiral limb as. c) Low power image of GFP fluorescence (white dots) in mesenchymal cells on Reissner's membrane. Alternating configuration CFE 40V - 20 pulses; 3 days organotypic culture. Background structure provided by autofluorescence acquired on a .separate channel . d) Detail of cells shown in c), with double fluorescence of GFP reporter and BDNF synthesis).

[0028 j Figure 10 shows roicroCT of the guinea pig skull showing the 8 electrode cochlear implant microarray in sifu within the lower turn of the cochlea, a) top down view; implanted (left) and control cochleae are indicated by arrowheads, b) detail of microarray within the basal turn of the left cochlea showing the 8 platinum ring electrodes, e) Tilted right-side view of skull showing the right (upper) and left (implanted) cochleae.

[0029] Figure 11 shows results for in vivo CFE of guinea pig cochleae using cochlear implant electrode microartays in alternating or tandem electrode configurations: a) Transduced mesenchymal cells (GFP +) in the. perilymphatic compartments (scala tyropani (ST) and scala vestibuli (SV)} 4 days post tandem configuration CFE - white arrowhead, RW - round window. 20 V - 5 pulses, b) Alternating configuration CFE: 20 V - 5 pulses, c) Tandem configuration CFE provided --8 times greater transformation efficiency than alternating array CFE (n=7 tandem, n=4 alternating). * P = 0.043 (t-test).

[0030] Figure 12 shows results for in vivo tandem configuration CFE of the BDNF- GFP gene construct, a) Fluorescence imaging of transformed mesenchymal cells lining the perilymphatic compartment of the cochlea (nuclear localization of GFP, white dots), with overall cell density determined by DAP! nuclear labelling (grey - via multi-photon excitation), b) The maximum transformation efficiency was determined by calculating the number of GFP+ cells / total cell number within a region of interest containing > 100 cells as outlined. CFE: 20V - 5 pulses; imaged 4 days post-treatment.

[0031] Figure 13 shows results for BDNF gene therapy in a deafened cochlea, a) Regeneration of spiral ganglion neuron (SGN) two weeks post CFE (alternating; 40V - 20 pulses). Type 01 β-tubuhn immunofluorescence of SGN, highlights restored neurites in the osseous spiral lamin (OSL), which extend beyond the habenula perforata (HP) and branch into scala tympani (ST) - arrowheads. SL - spiral iimbus; SM - scala medi (endolymph); SV - scala vestibuli. b) Control (right) cochlea from the same guinea-pig; contrasting the loss of peripheral neurites in the OSL and atrophy of the SGN somata. Right-side images provide detail of the basal region. Grey in (a) (left-hand side), and (b) (left-hand side) is autofluorescence, used to provide cochlear structure; grey in (a) (right-hand side) and (b) (right-hand side) shows fluorescence from DAPI nuclear staining, c) Aspartoacylase (ASPA) imnjoiiofluorescence (white) showing satellite cell myelination of ^* the regenerated neurites in a BDNF treated cochlea, d) Neurite regeneration with gene therapy. Data are mean (± s.e.m.); β-tubulin immunofluorescence pixel intensity (average sum of pixel intensity μχα ^* ) within the OSL; * P = 0.017, paired t-test. (n=4). e) Increase in soma size of BDNF-treated SGN; comparing cross-sectional areas of neuron somata (n = 4) (* P = 0.012,. paired t-test). 50 pro cochlear cryoseetions.

[0032] Figure 14 shows results for BDNF gene therapy using tandem configuration CFE. a) Type III B-tubulin immunofluorescence of the hemi -sectioned cochlea two weeks after electroporation (20V - 5 pulses) (4 weeks post-deafening). Confocal 3D reconstruction shows the spiral ganglion neurite regeneration back along the osseous sptral lamina (OSL), through the habenula perforata (HP) and extending to the inner sulcus (IS) region, under the lateral prominence of the spiral imbus (S L). Cochlear structure shown by autofluorescence. b) Detail of the same preparation showing the ectopic branching of the regenerated neurites. Nuclear labelling (DAPI) - grey signal, c) Control (deafened, no gene therapy) right cochlea from the same guinea pig.

[0033] Figure 15 illustrates examples of regeneratio of the spiral ganglion peripheral neurites in vim following CFE-mediated BDNF gene therapy i deafened guinea pigs, a) Image from a cryosection (50μτη) showing peripheral spiral ganglion neurites, extending back through the osseous spiral lamina (OSL) to the former site of the hair cells, now a flattened basilar membrane lacking hair eels and supporting cells immediately to the right of the habenula perforata (HP). (3 weeks post ototoxic treatment). The neurites are shown branching at the HP, to project medially to the inner sulcus region (IS) and laterally to the oute sulcus (OS) and spiral ligament (Sf ,g). Endolymphatic compartment (scala media, SM). Fibres also project into scala tympant (ST). SGN, spiral ganglion neurons; SV, scala vestibuli. Alternating configuration CFE: 40V - 20 pulses, 7 days post-treatment. DAPI unclear fluorescence evident in background, b) Detail of outgrowth of fibres in the OSL, with ectopic branching of fibres into ST, both at the HP, and directly from the OSL (arrowheads). Cryosection (SOpm). CFE: alternating configuratio 40 V - 20 p; 1.1 days post-treatment (25 days post ototoxic treatment).

[0034] Figure 16 shows functional validation of enhanced cochlear implant performance following BDNF gene therapy via tandem configuration CFE (20V - 5 pulses; deafened guinea pig model), a) eABR traces after BDNF gene therapy; note the lower current stimulus needed to elicit a threshold response (88 μΑ - left arrowhead) compared to the threshold level of a control (CFE with a GFP-oniy vector) animal (445 μΑ - right arrowhead); two weeks post CFE gene delivery; four weeks post deafening, b) Individual eABR input-output functions (pl -n i amplitude = output) for BDNF-treated versus control (n = 5 for each). Note the lower thresholds and broader dynamic range of the eABRs with BDNF gene therapy, c) eABR thresholds. The mean threshold (dashed line) of the BDNF treatment group was less than half that of the control group. *** P<0.Q01, t-test. d) Average eABR input-output growth functions. Linear regression best-fits: BDNF group: y - a + bx _? where a - -4.45, b=0.026. R ² = 0.957; Control group: y= a + bx, where a = -6.64, b = 0.021, R ² - 0.979. *** PO.QQl, ranked two way ANGVA.

[0035] Figure 17 shows control histology and eABR data, a) Immunofluorescence (anti- -tub Iin) of a cryosection from an untreated guinea pi cochlea, SGN - spiral ganglion neurons; OSL - osseous spiral lamina; HP - habenula perforata; SM - scala media; ST - scal tympani; SV - scala vestibuli; SL - spiral limbus. Left-hand side - autofluorescence channel fo structural detail; Right-hand side - DAP.I nuclear labelling in background, b) β-tubuiin immunofluorescence from a cochlear cryosection 2 weeks post- ototoxic deafening. This is the point where CFE gene therapy was performed, c) Box plots with overlaid data showing eAB thresholds for acute cochlear implants, and implants inserted 2 weeks post deafening (n = 5 acute; n = 10 deafened). Dashed lines are the means, d) Average eABR input-output functions for baseline and deafened states.

[0036] Figure 18: Follow-up eABR data on CFE -mediated BDNF gene therapy at 4 weeks post implant (6 weeks post-deafening). Note that the stimulus thresholds and growth functions of the BDNF-treated animals approach the baseline data (see Fig. 18). Linear regressions: BDNF treatment: y = -3.227 + 0.032*x, R ² = 0.981 ; GFP control: y = -4.121 + 0.0 Ϊ 86*x, R ² = 0.971 , (n = 2 BDNF treated, n = 4 control).

[0037] Figure 19 shows the efficiency of transformation of HEK293 cells with a GFP reporter cDNA construct as a function of length of electroporation pulse (40V and 5 pulses).

[0038] Figure 20 shows A) Cochlear implant anode and cathode array configurations employed in Examples 2 and 8; and B) a photomicrograph of an actual 8-ttode Cochlear implant linear electrode array (aligned with the array configuration representations). [0039] Figure 21: Nuclear localised GFP fluorescence indicates HEK293 ceils transformed by CFE, A, C-F (B=control), show examples of different electrode array configurations.

[0040 | Figure 22: Summary of data obtained from experiment illustrated in Figure 21, indicating the mean +/- SEM number of transformed cells per experiment. * indicates P<0,02; ** P<O. ^Q 01 ; ranked ANOVA with Holm-Sidak post-hoc pairwise comparisons, B, Control, plasmid DNA with the electrode array, without CFE. Position of electrode is overlaid schematically. All experiments utilised 40V, 10 pulses, 50ms duration.

[0041 J Figure 23 shows the effect of voltage amplitude on cell transformation with 'tandem ^* electrode configuration CFE. CFE was delivered using 10 pulses at 40 ms duration, with varying voltage. Summary of data, indicating the mean +/- SEM number of transformed cells per experiment. * indicates P<0.05: ranked ANOVA with Tukey's test post-hoc pairwise comparisons. All experiments utilised 40ms duration, 10 pulses. 1/s.

[Θ042] Figure 24 shows the effect of pulse number of transformation efficiency with 'tandem' electrode con figuration CFE, CFE was delivered using 20 and 40V with 40 ms duration, with varying pulse number. Summary of data, indicating the mean +/- SEM number of transformed cells per experiment. * indicates P<0.001 and ** P<0.O5; ranked two-way ANOVA with Holm-Sidak method post-hoc pairwise comparisons.

[0043] Figure 25 shows the effect of increasing pulse number on cell death. A. Image showing dead cells labelled with propidium iodide 30 rain after Tandem CFE (40 pulses, 40 V, 40ms) localised close to the electrode array. B. Propidium iodide fluorescence at 30 mins post-CFE with 5 pulses. C. Propidium iodide fluorescence at 30 rains post-CFE with.

1 pulse. D. Propidium iodide fluorescence at 30 mins with no-CFE (control).

[00441 Figure 26 shows the effect of pulse duration on number of transformed cells with I or 5 pulses via 'tandem' electrode configuration CFE, with 40 V and varying pulse duration. Summary of data indicating the mean +/- SEM number of ^• transformed cells per experiment. * indicates P<0.001 and ·** P<0.05; ranked ANOVA with Holm-Sidak method post-hoc pairwise comparisons. One pulse per second was delivered for the 5 pulse test, Cntrl; no CFE control.

[0045] Figure 27 shows the effect of gap duration on number of transformed cells with

2 or 5 pulses via 'tandem' electrode configuration CFE, with 40 V pulses 40 ms in length and varying gap duration. Summary of data indicating the mean +/- SEM number of transformed cells per experiment. [Θ046] * indicates P<0.001 and ** P<0.05; ranked ANO ^' VA with Holm-Sidak method post-hoc pairwise comparisons. Cntrl; no CFE.

Definitions

[D047| Throughout this specification the word "comprise", or variations such as "comprises" or "comprising", will be understood to imply the inclusion of a stated element, integer or step, or group of elements, integers or steps, but not the exclusion of any other element, integer or step, or grou of elements, integers or steps.

[00481 Throughput this specification, reference to "a" or "one" element does not exclude the plural, unless context determines otherwise. For instance, reference to "a nucleic acid molecule" should not be read as excluding the possibility of multiple nucleic acid molecules.

[0049] As used herein, the term "close electric field" refers to an electric field formed between a positive electrode (anode) or anode array and a negative electrode (cadiode) or cathode array, wherein the cathode or cathode array and the anode or anode array are separated by between about lOpm and about 5mm or less, t pically between about SOpro and about 1mm or less, and even more typically between about 50um and about 0.5mm or less, where the distance between the cathode or cathode array and the anode or anode array is measured as the distance between the proximal edges of the closest cathode and anode pairing.

[0050] As used herein, the term "subject" refers to any organism that may benefit from transaction of an agent into one or more of its cells, or an organism the transfection of which is desired for any other reason, such as to modify the organism's performance under certain conditions, or its ability to utilise or tolerate certain substances. According to an embodiment the subject is an animal, such as a mammal, including, but not limited to any one of humans, non-human primates, dogs, eats, horses, rodents, and the like. Typically, the terms "subject" and "patient" are used interchangeably herein. Typically the subject is a human.

[0051] As used herein, the term "non-human animals" refers to non-human vertebrate animals including, but not necessarily limited to, rodents, non-human primates, ovines, bovines. ruminants, lagomorphs, porcines, caprines, equines, canines, felines, aves, etc.

[0052 J As used herein, the term "nucleic acid molecule" refers to any nucleic acid containing molecule, including but not limited to D A or RNA molecules. The term encompasses sequences that include any of the known base analogs of DNA and RNA including, but not limited to, 4-acetylcytosine, 84iydroxy-N6-raethyladenosine, azirid yicytosme, pseudoisocytosine, 5 -(c arboxy hydroxy I m ethyl )uraci 1 , 5- carboxymethylaniinomethyl-2-thiouracil, 5-carboxymethylammomethyIuracil- dihydrouracil, inositie, N6-isopentenyladenrae, 1 -metoyladenine, 1 -methylpseudouracil, i- methy!gaanine,, 1 -methylioosine, 2,2 -dimethyl guanine, 2-methyladenine, 2-roethylguanine, 3-methyicytosine, 5 -methyl eytosine, Νό-methylade ne, 7-memy! guanine, 5- methylamittomethyiuraci!, 5-methoxyaniinomethyl-2-thiouracii, beta-D-mannosylqueosine, 5 -metfioxycar bony! -metbyluracil, 5-metfaoxyuracil, 2-methylthio-N6-isopentenylademne., uracil -5-oxyacetic acid methylester, uracil-5-oxyacetic acid, oxybutoxosine, pseudouracil, queosine, 2-thiocytosine, 5-methyl-2-thiouraci1. 2-thiouracil, 4-thiouracil, 5 -methyl uracil, N- uracil -5-oxyacetic acid methylester, uracil -5-oxyacetic acid, pseudouracil, queosine, 2- thiocytosine, and 2,0-diaminopurine, A nucleic acid molecule may include modifications designed to improve delivery into cells, stability once inside a cell, and/or binding to the appropriate intracellular target.

[0053] Th nucleic acid molecule may be an oligonucleotide or a polynucleotide. The nucleic acid molecule may be naked DNA, such as cDNA, or naked RNA. The nucleic acid may comprise encapsulated DNA or RNA, such as polynucleotides present in liposomes or microspheres, particularly where such encapsulation improves the stability of the agent or the efficiency of transfeclion b electroporation. The use of encapsulated DNA or RNA may offer a less advantageous embodiment if encapsulation allows transection, of cells which are distant to the electrode to take place. It will be understood by a person skilled in the art that particles may be mixed with the nucleic acid molecule to condense the nucleic acid molecule, which may provide increased transduction efficiency.

|0054] The terms "in operable combination," "in operable order," and "operably linked" as used herein refer to the linkage of nucleic acid molecule sequences in such a manner that a nucleic acid molecule capable of directing the transcription of a given sequence and/or the synthesis of a desired polypeptide product is produced. The term also refers to the linkage of amino acid sequences in such a manner so that a functional protein is produced.

|0055] The terms "expression vector" or "expression cassette", as used herein, refer to a recombinant DNA molecule containing at least one desired coding sequence and appropriate nucleic acid sequences necessary for the expression of the operably linked coding sequence in a particular host organism. Nucleic acid sequences necessary for expression in eukaryotic cells are known to utilize promoters, enhancers, and termination and polyadenylatio signals. [Θ056] As used herein, the term "transfection" refers to the transfer of an agent, including but not limited to a nucleic acid molecule, a peptide or a protein, into the cytoplasm and/or nucleus of a cell by electroporation. The agent may be a nucleic acid molecule, and may encode a polypeptide product which is expressed by the cell. Alternatively, the nucleic acid molecul may act upon the transcription and translation machinery of the cell. A. transfected cell may carry the nucleic acid molecule product ^■ permanently if the nucleic acid molecule agent is stably incorporated into the cel l 's genome. A transfected cell may cany the nucleic acid molecule product transiently if the nucleic acid molecule agent is not incorporated into the cell's genome, or the expression cassette and vector may retain expression viability but may not integrate into the chromosomal DMA (episomal expression) .

Detailed Description

[0057] The present invention is based on studies which surprisingly found that certain configurations of cochlear implant electrode microarrays, resulting in small intense electrical fields occurring at the cellular level, resulted in surprisingly more efficient and more targeted electroporation and resulting transfection, compared with previously published studies. In particular, high transformation efficiencies were obtained at voltages as low as 10V and at significantly reduced total delivered charge.

[0058] These results now show that highly efficient and highly targeted transfection of cells in vivo is possible, allowing for bioactive agent therapy, such as gene therapy, targeted at specific groups of cells within a subject with minimal effects on surrounding ceils. Such methods may find application in control, reversal, compensation for, and possibly even cure of neurological conditions or defects including nerve degeneration or damage of a cerebral , spinal, otological, optical or other sensory nature, and/or other physiological conditions or defects affecting the muscoskeletal, cardiovascular, respiratory, gastrointestinal, integumentary, urinary, reproductive, immune or endocrine systems. ^' The methods may be utilized to deliver targeted cancer treatment. Because of the spatial specificity and efficiency of the methods of the present invention, particular areas of the brain or spinal nerve may be more readily and safely treated, and conditions not previously considered treatable may now be addressed.

[0059] The methods described herein allow the use of targeted and efficient gene therapy which may, for example, enhance the expression of spiral ganglion cell trophic molecules, such as brain-derived neurotrophic factor, in cochlear cells which lie close to the cochlear implant. This is proposed to be beneficial to the functio of the cochlear implant by promoting spiral ganglion cell neurite outgrowth specifically towards the transformed cells, which in tur bring die spiral ganglion neuron processes in close proximity to the cochlear implant electrodes. By providing spiral ganglion neurite outgrowth close to the position of the cochlear implant, the functkmality of the cochlear jmplant may be improved by lowering the required, stimulation currents. The use of lowered stimulation currents may enable the electrode design of the cochlear implant to include a higher density of electrodes when working as a bionic ear, thus also improving the auditory information bandwidth, that can be delivered to the cochlear nerve.

Electroporation

[0060] The methods described herein use close electric field to deliver an agent into the cytoplasm or nucleus, or both, of cells within a restricted target region.

[0061] The close electric field arises from the proximit of the electrodes used, such as micron-sized electrodes within cochlear microarrays, and the resulting uniformity and intensity of the resulting electric field for a given voltage.

[0062] For the purposes of e!ectroporation a cathode and an anode is required. The cathode and anode may be introduced to the target region fo treatment as separate electrodes or as components of a single device, such as an electroporation probe. The cathode and/or anode may, independently, also comprise a single electrode or a plurality or array of electrodes.

[0063] The cathode or cathode array and the anode or anode arra may be separated by between about 10pm and about 5mm, such as between about Ι μ ^η ι and about 4mm, between about 10pm and about 3mm, between about Ι Ομηι and about 2mm, between about Ι Ομηι and about 1.5mm, between about ΙΟμηι and about Irani, between about IOum and about 0.75mm, between about IQpm and about 0.5mm, between about IQpm and about 0.4mm, between about I Oum and about 0.3mm, between about ΙΟμιη and about 0.2mm, any of the foregoing ranges in which the lower limit is 50μπι instead of .1 Ομιη or any of the foregoin ranges in which the lower limit is iOOpm instead of ΙΟμηι, between about ΙΟμηι and about 0.1mm, between about ΙΟμηι and about 50pm, between about ΙΟμηι and about 25μηα, about ΙΟμηι, about 25μιη, about 50μηι, about 75μηι, about 0.1mm, about 0, 15mm, about 0.2mm, about 0,25mm, about 0.3mm, about 0.35mm, about 0.4mm, about 0.45mm, about 0.5mm, about 0.55mm,. about 0.6mm, about 0.65mm, about 0.7mm, about 0.75mm, about 0.8mm, about 0.85mm, about 0.9mm, about 0.95mm, about Imm, about 1.5mm, about 2mm, about 3 mm, about 4mm or about 5 mm. [0064] The cathode, or electrodes in the cathode atray, and the anode, or electrodes in the anode array, may comprise electrically conductive, non-toxic or bio-inert metal, for instance platinum, gold, tungsten, or stainless steel The electroporation electrode may be electrically conductive over or along its entire surface. Alternatively, the electroporation electrode may be electrically conductive at its surface along one or more portions of the entire length of the electrode, and electrically non-conductive at its surface along another portion or portions of its length. The non-conductive portions may be insulated at die surface of the electrode with a coating, such as a resin or plastic coating. The non- conductive portions may be made of a non-conducting material, such as a resin or plastic. Conductive and non-conducti ve polymer materials and hydrogel materials may also be used. This may include molecular pore size determination to provide appropriate spatiotemporal delivery of the DMA within the effective range for close-field electroporation. The eleetrode(s) may be 'point ¹ source(s) or sink(s) connected to power source(s) via insulated conductiv lines. The electrodes may be of any desired shape, such as square, spherical, circular, oblong or elongated of any of the foregoing, cylindrical, etc. in an embodiment, each electrode may be, independently, less than 1mm in one or more dimensions and ma have one or more dimensions in the nanometre range (Including less than 5nm). Typically, the electrode(s) may have one or more dimensions of less than 0.75mm, less than 0.5mm, less than 0.4mm, less than 0.3mm, less than 0.2mm, less than O. lmm, less than about 50μηι, less than about 25um _: . less than about l Opm, less than about 5 τη, less than about l pm, about 0.025rara, about 0.05mm, about 0.075mm, about 0.1mm, about 0.125mm, about 0.15mm, about 0.2mm, about 0.25mm, about 0,3mm, about 0,35mm, about 0,4mm, about 0.45mm, about 0,5mm, about 0,55mm, about 0.6mm, about 0.65mm, about 0,7mm, or about 0.75mm. In an embodiment, all dimensions of an electrode for use in a method according to the present invention comply with the above listed dimensions and, in a further embodiment, all dimensions of all electrodeCs) for use in a method according to the present invention comply with the above listed dimensions.

[0065] The cathode or cathode array and the anode or anode array may be configured in any convenient manner allowing for the separations described herein, and configurations may include a single cathode and anode, a single cathode and a plurality of anodes, a single anode and a plurality of cathodes, or a plurality of anodes and a plurality of cathodes. The cathode or cathode array and the anode or anode array may be counterpositioned on opposite sides of the target region for electroporation, or the cathode or cathode ar ray and the anode or anode array may be placed end to end such that the close electric field flows along an axis defined by the cathode or cathode array and the anode or anode array. Another potential GOO figuration contemplated by die present invention comprises a cathode or anode array comprising a plurality of electrodes arranged around one or a few electrodes of opposite polarity; An example of such an arrangement may comprise a hexagonal array of electrodes surrounding or in the proximity of a single electrode of opposite polarity as illustrated in Figure 2B. This is described as a planar array.

[0066] The electrodes or arrays thereof may be inserted into or next to the target region before or after the agent is introduced into the target region,

[0067] In certain embodiments of the methods described herein, at least one electrode which is used tor electroporation is provided by a cochiear implant. The term "cochlear implant" or "cochlear implant device" as used herein refers to a device which is at least partially implanted in the sca!a tympani or the scala vestibuli of the cochlea for the purpose of restoring hearing by direct electrical stimulation of the cochlear nerve. A cochlear implant is commonly termed a "htotvic ear". Examples of cochlear implants and/or electrodes for cochlear implants are described in US Patent No. 7,451.000 (Cochlear Limited), US patent No. 7,406,352 (Cochlear Limited); US patent No. 7,367,992 (Cochlear Limited), US patent No. 7,349,744 (Cochlear Limited); US patent No. 7,346,397 (Cochlear Limited), US patent No. 7,340,308 (Advanced Cochlear Systems, Inc.), US patent No. 7,319,906 (Advanced Bionics Corporation), US patent No. 7,317,944 (Advanced Bionics Corporation), and US patent No, 7,315,763 (Advanced Bionics Corporation), the entire contents of each of which is incorporated by reference.

[0068] A cochlear implant possesses at least one electrode and more typically a series of independently addressable electrodes along the length of the region of the implant lying within the cochlea, with each electrode electrically insulated from other electrodes, forming a microarray. An example of a cochiear implant used in proof of principle studies here has a linear implant electrode microarray comprising 8 platinum ring electrodes, each having a diameter of approximately 400μιη and a depth of approximatel 350um, each separated by a space of approximately 350μηα, for a total microarra length of just over 5mm. During the normal operation of the cochlear implant, the electrodes receive stimuli originating from an external control unit and provide an electrical stimulus to the spiral ganglion cells by creating a voltage between the electrodes in a btmodal fashion to prevent polarization of the electrodes. In this normal neural interface mode, the stimulus to the nerve fibres may be unipolar, with one or more electrodes delivering current to a distant reference electrode, or bipolar, wit current return between various arra electrodes. [0069] For tire electroporation methods described herein, in certain embodiments some or all of the electrodes of the cochlear implant may act as a single electrode. In other embodiments, individual electrodes or groups of electrodes may act as an electrode array and other individual electrodes or groups of electrodes may act as a second electrode array. In certain embodiments the cochlear implant may provide a plurality of electrodes and second electrodes. Examples of configurations of electrodes and/or dedicated electroporation electrodes on a cochlear implant are illustrated in Fig. I d and Fig. 4.

[0070] According to an embodiment the distal four electrodes form an electrode array of one polarity and the proximal four electrodes form an electrode array of opposite polarity in a 'tandem' mtcroartay,

[0071 [ In an alternative embodiment, all the electrodes, or specific electrodes along die implant, may be used to generate a transient dielectric breakdown of the lipid b layer of the plasma membrane of the target cells. In one embodiment, this may be performed by simultaneously polarizing some or all of the electrodes relative to at least one separate second electrode which is located close to the cochlear implant mieroarray. to provide a current return path. This second electrode is typically the cathode (negative) terminal rel tive to the electrodes of the electrode array.

[0072] Electrode arrays comprising interspersed electrode couplings, such as alternating arrays in which anodes and cathodes alternate with one another, may result in interference between the respective fields, possibly resulting in less intense or more dispersed electric fields compared to fields generated by discrete anode and cathode electrodes or between discrete anode and cathode arrays, and may result in less efficient transformation and poorer specificity of localisation of transformation. Accordin to an embodiment, interspersed electrode couplings, such as alternating arrays are excluded from the scope of the present invention. According to another embodiment, the electrode arrays are configured as distinct or separated polarities.

[0073 J Current may be delivered to and from the electrode(s) via connection to external (ie. outside of the subject) electroporation circuitry. Alternatively, the current for electroporation may be provided by dedicated circuitry in an electrode-bearing implant, such as a cochlear implant. In such embodiments the dedicated circuitry generates the required electrical pulse profile in an electroporation mode for use during the implantation procedure and at later stages if additional gene transleetion is performed- The implant stimulator may then be switched to a conventional stimulus mode for operation as, for example, a cochlear prosthesis / auditory nerve stimulator. In certain embodiments dedicated electroporation electrodes included in an implant are not used in the normal operation of the implant.

|0074] In certai embodiments, cochlear implants are provided in which one or more dedicated electroporation electrodes are built into the implant for the purpose of providing the electroporation. stimulus to the cochlear tissue. These dedicated electrodes may be driven via an external isolated electroporatkm-drivin circuit, or the one or more dedicated electroporation electrodes could be driven externally by electroporation circuitry. In certain embodiments the dedicated electroporation electrodes are not used in the normal operation of the cochlear prosthesis in providmg direct electrical stimulus to the spiral ganglion cells as a result of auditory stimulation.

[0075J The electroporation stimulus is typically provided in a square wave voltage configuration, delivering from a 0 Volt reference about IV to about 100V, such as from about IV to about 75V, about IV to about 50V, about I V to about 40V, about IV to about 30V, from about I V to about 25V, from about I V to about 20V, from about I V to about 15V, from about I V to about 10V, from about IV to about 5V, from about 5V to about 50V, from about 10V to about 50V, from about 15V to about 50V, from about 20V to about 50V, from about 25V to about 50V, from about 30V to about 50V, from about I0V to about 50V, from about 10V to about 40V. from about 10V to about 30V, about 5V, about lOV, about 15V, about 20V, about 25V, about 30V, about 35V, or about 40V.

[0076] Currents applied in the course of electroporation methods according to the present invention may be controlled or uncontrolled and will depend on the material within which the method is being carried out (as different, materials will have different resistances), the area or volume over which the electric field is spread (which will depend on the configuration and shape of the anode and or cathode or arrays thereof) and will also depend on whether anodes and cathodes are interspersed such that interference between electric fields occurs or not (which will increase the current required for a given electric field strength). For a linear anode and cathode array configuration, for example as illustrated in Figure 3 or equivalent, it may be desirable to deliver the electroporation current using current sources rather than voltage sources. In this case the current supplied to each electrode may be from about Ι Α to about 150mA, such as from about 1mA to about 100mA, from about 1mA to about 75mA, from about 1mA to about 50mA, from about 1mA to about 40mA, from about 1mA to about 30mA, from about 1mA to about 25mA, from about 1mA to about 20mA, fro about 1 mA to about 15mA, from about 1mA to about 10mA, from about 1mA to about 5mA, from about 2mA to about 50mA, from about 5mA to about 50mA, from about 10mA to about 50mA, from about 15mA to about 50mA, from about 20mA to about 50mA, from about 30mA to about 50mA, from about 5mA to about 40mA, from about 5mA to about 30mA, from about 5mA to about 20mA, from about 10mA to about 20mA, about 1mA, about 2mA, about 3mA, about 5mA, about 7mA, about iQmA, about 15m A, about 20m A, about 25mA, about 30mA, or about 40mA.

[0077] Total charge (Coulombs) applied in the course of electroporation methods according to the present invention may also be controlled or uncontrolled and as per current, described above, will depend on the material within which the method is being carried out, the area or volume over which the electric field is spread (which will depend on die configuration and shape of the anode and or cathode or arrays thereof) and will also depend on whether anodes and cathodes are interspersed such that interference between electric fields occurs or not (which will, increase the current required to generate a given electric field strength). For a linear anode and cathode array configuration, for example as illustrated in Figure 3 or equivalent, the charge delivered may be from about 1 μ€ to about 1C / electrode per pulse. The charge may depend upon the number of electrodes, voltage and pulse width. Expressing this as per electrode per pulse enables the addressing of very broad operational ranges - for example l ,u is the charge delivered to each of four of the 8 electrodes in one polarity at 4 Volts for 100 μ$. Ignoring the roughness factor (the fine 3- dimensional structure of platinum electrodes), the minimum effective charge density on platinum electrodes may be between about ImC/cnr and about lC/cm", such as between about SmC/crr and about 500mC/cm ² , between about lOmC/cra" and about 400m€/cnV ⁱ , between about 15m.C/cn and about 350mC/cnr, between about 20mC ctrr and about 300mC/cm% between about 25mC/cm ^" and about 250mC/cm% between about 30mC/cm ^" and between about 40rnC/cnr and about 150mC/cm ⁱ , between about

50mC/cm' and about lOQmC/cnr, about l QmC cm\ about 15mC/cm\ about 20mC/cm ^z , about 25GmC/cm ² , about. 30mC/cm ² , about 40mC/cm ² , about 50rnC cm ² , about 60mC cm , about 70mC/cm ² , about 80mC/cm ^" ., about lOOmC/cm ² , about 120mC cm ² , about 150mC/crn ^a , about 200mC cm ² , or about 250mC/cm ² .

[0078] The electroporation stimulus may be delivered as pulses of from about 100μ5 to 10 s duration, such as f om about 1ms to about 500ms, from about 1ms to about 300ms, from about 1 ms to about 200ms, from about 1 ms to about 150ms, from about 1ms to about 100ms, from about 1ms to about 75ms, f om about 1 ms to about 50ms, f om about 0.1ms to about 5ms, from about 5ms to about 25ms, from about 20ms to about 50ms, from about 50ms to about 100ms, from about 50ms to about 200ms, from about 50ms to about 300ms, from about 50ms to about 500rns ₅ about 10ms, about 20ms, about 30ms, about 40ms, about 50ms, about 60ms, about 70ms, about 80ms, about 90ms, about 100ms, about 150ms, or about 200ms.

[0079] Multiple pulses are commonly used, with typically from 1 to 500 pulses, such as 1 to about 400 pulses, from i to about 300 pulses, from 1 to about 200 pulses, from 1 to about 100 pulses, from 1 to about 75 pulses, from 1 to about 50 pulses, from 1 to about 40 pulses, from ί to about 30 pulses, from 1 to about 20 pulses, from 1 to about 10 pulses, 2 pulses, about 5 pulses, about 10 pulses, about 15 pulses, about 20 pulses, about 25 pulses, about 30 pulses, about 50 pulses, about 75 pulses, about 100 pulses, about 20 pulses, about 300 pulses, about 500 pulses.

[0080j The interval between electrostimulation pulses may be from about 0.1 to about 30 seconds, such as from about 0.1 s to about 20s, from about 0.1 s to about 10s, from about 0.1s to about 5s, from about 0. s to about 4s, from about 0. 1s to about 3s, from about 0.1 s to about 2s, from about 0.1 s to about 1 s, from about 0. Is to about 0.5s, from about 0. is to about 0.3s, from about 0.1s to about 0.2s, from about 0,5s to about Is, from about 0.5s to about 2s, from about 0.5s to about 5s, about 0.5s, about 0.75s, about Is, about 1 ,5s, about 2s, about 3s, about 4s, about 5s, or about 10s.

[00811 The delivery of the electroporation current during the electroporation procedure for delivery of an agent may last from about 100 _t us to about 20 minutes, depending upon the number of pulses and delay between pulses if more than one pulse is applied.

[0082] Ramped waveforms may also be used, as these may provide a faster onset of electroporation dielectric breakdown of the cell membrane for agent delivery, while minimizing current delivery. As such stimuli delivered to the intact cochlea of a subject may be perceived by the subject as excessively loud noise, typically the subject will be under general anaesthetic during electroporation.

[0083] According to an embodiment of the present invention, the electroporation stimulus is provided in up to 40 pulses of about 50 to about 100ms duration separated by intervals of from about 0.5 to about 2 seconds, such as pulses of 50nis duration separated by intervals of 950ms, and delivered in a square wave configuration, at a voltage of from 10 to 40V, with a current Of up to about 20mA and total charge of less than 1 OOmC, with the gap between the cathode or cathode array and the anode or anode array being up to about 1 mm, more typically less than 0.5mm. [Θ084] In addition to the above, the positive (anode) and negative (cathode) arrays may he switched within an electrode array between pulses o groups of pulses. It is contemplated that this may allow for shapin of fields for electroporation and DNA transfer in 3 dimensional space.

Subjects and target regions

[0085| The present invention contemplates the transfection of any cells within any target region. The target regio may be inside or outside an organism. According to an embodiment the target region is in or on human or non-human organism, such as within an organ inside a hitman or non-human animal. In another embodiment, the target region may be a region on or in a surface or volume comprising cells to be transfected such as, for example, a region on or in a medium in a Petri dish or other culture vessel, a region in an explanted organ. According to embodiments, the present invention finds its main application in transfection of cells within a target region in a human or non-human animal According to an embodiment, the subject is human. According to another embodiment, the subject is a non-human mammal, such as, but not limited to, dog, cat, horse, or rodent.

[ 086 [ In embodiments, the spacial accuracy and transformation efficiency of electroporation methods of the present invention will find application in a wide range of treatments of human and non-human animals, especially in correction or repair of defects or damage in localised regions in said animal(s). Non-limiting examples may include transfection of otic cells, soch as cochlear mesenchymal cells, or nerve-or nerve-associated cells, including brain cells and optic cells (retina, optic nerve) to express neurotrophic factors to encourage or restore growth of nerves or re-myelination of cells to impro ve, establish or re-establish neural connections. Such methods may be for treating, correcting, halting or slowing down progressio of or onset of. or at least ameliorating conditions including deafness, muscular dystrophy, Parkinson's disease, or spinal injury. Other examples may comprise transfection of particular cell types within an organ, such as the skin, skeletal or smooth muscle, liver, kidney, heart, pancreas, gastrointestinal system, reproductive organs, or the central and peripheral nervous systems, including the autonomic and enteric nervous systems, or a tumour, to provide, correct, improve or inhibit an activity in the target region. Methods according to the present invention may also find application in, or association with bionics other than cochlear implants.

[0087[ Ceils of particular interest include post-mitotic cells, such as neurons and sensory cells, such as cochlear hair cells, which may not be replaced by the body. Data reported herein indicates that functionality may be restored to such cells by methods according to the present invention comprising somatic cei l gene therapy.

10088] Electrodes or electrode arrays may be purpose-designed for particular applications or may be reconfigured electrodes of a cochlear implant electrode microarray. For transfection of cells within target regions well within the body of a subject, or well withi an organ inside said subject, a long, thin/ arrow shaft is contemplated as a embodiment, with the electrodes arranged at the tip either transversely or axiaily. In other methods, planar mieroarrays consisting of sheets of electrode nodes would be matched to the region targeted for close-field electroporation for delivery of the therapeutic molecules.

[0089] Where the methods described herein are intended for treatment of cochlear cells, the cochlea may be present in situ within a live subject. Where the methods described herein are intended for experimentai or research purposes the cochlea may be present in situ within a live subject or may be substantially intact but isolated f om the subject.

[0090] Cells which may be transfected by the methods described herein may be cells lying within less than 5mm of an electrode, such as less than 4mm, less than 3mm. less than 2mm, less than 1.5mm, less than 1mm. less than 0.75mm, less than 0.5mm, less than 0.4mm, less than 0.3mm, less than 0.2mm, less tha 0. 1mm, less than about 50μιη, about 50μιη, about 0.1 mm, about 0.15mm, about 0.2mm, about 0.25mm, about 0.3mm, about 0.35mm, about 0.4mm, about 0.45mm, about 0.5mm, about 0.6mm, about 0.7mm, about 0.8mm, about 0.9mm, about Irani, or about 2mm. According to an embodiment, cells transfected by the methods described herein are within 3mm of an electrode. According to an embodiment, celts transfected by the methods described herein will be cochlear mesenchymal cells.

[0091] In particular embodiments a subject's cochlea is a mature cochlea, which as used herein refers to a developmental stage of the cochlea within which air-borne sound transduction takes place. In rodents such as rats, mice and guinea pigs, this typically occurs around one week after birth, in humans air-borne sound transduction commences at birth.

[0092 [ Methods according to the present invention may be for transient expression of an agent by the cells within the target region, or may be for transfection and integration of a desired nucleic acid into the cell's genome for permanent transfection, or permanent expression of the expression vector without integration. Expression of a nucleic acid transfected into a particular cell or cell type in a target region may be inducible or constitutive, depending on the regulator regions, such as promoters, associated with the nucleic acid, or by direction through manipulation of the close electric field properties. Agen s

Nucleic Acid Molecules

|i)093] In certain embodiments, the agent may consist of a nucleic acid molecule. In certain embodiments the agent may comprise a nucleic acid molecule, in which case the agent may, for example, also comprise molecules which enhance the stability or transfection efficiency of the nucleic acid molecule, such as chitosan, and/or may also comprise non nucleic acid molecules such as small neuroprotective peptides.

(a) Nucleic acid molecules which encode a polypeptide product

[00941 The agent may be a nucleic acid molecule which encodes a polypeptide product. The expression of the encoded polypeptide product promotes a desired outcome, such as promoting or maintaining neural outgrowth and/or survival.

[0095] The encoded polypeptide product may be a polypeptide endogenous to the subject, in certain embodiments the encoded polypeptide product is an exogenous polypeptide.

[0096] The nucleic acid molecule which encodes a polypeptide product comprises a coding sequence for a polypeptide product or a precursor polypeptide of the polypeptide product. The polypeptide product may be a full-length polypeptide which occurs in nature, or it may be a portion of a polypeptide which occurs in nature, provided that the desired activity or functional properties (such as receptor binding and/or the ability to induce signal transduction) of the polypeptide product are retained. The nucleic acid molecule may also comprise a sequence located adjacent to the coding sequence on the 5' or 3' end of the coding sequence or on both the 5' and 3 ^* ends for a distance of about 1 kb or more on ei ther end such that the sequence corresponds to the length of a full-lengt mRNA sequence. Sequences located 5* of the coding region and present on the mRNA are referred to as 5' non-translated sequences. Sequences located 3' or downstream of the coding region and present on the mRNA are referred to as 3' non -translated sequences.

[0097| The nucleic acid molecule may comprise a cDNA sequence encoding a polypeptide product. The nucleic acid molecule may comprise a genomic sequence encoding a polypeptide product. A genomic sequence contains the coding region interrupted with non-coding sequences termed "introns" or "intervening regions" or "intervening sequences " Introns are segments of a gene that are transcribed into nuclear RNA (nRNA); introns may contain regulatory elements such as enhancers, !ntrons are removed or "spliced out" from the nuclear or primary transcript; introns therefore are absent in the messenger RNA (mRNA) transcript. The mRNA functions during translation to specify the sequence or order of amino acids in a nascent polypeptide.

|0098| In certain embodiments, the expression of a nucleic acid molecule refers to the process of converting genetic information encoded in the nucleic acid molecule into RNA (including for example mRNA or snRNA) through "transcription" of the nucleic acid molecule (i.e., via the enzymatic action of an RNA polymerase), and for nucleic acid molecules encoding a polypeptide, into a polypeptide product through translation of .mRNA. Expression ca be regulated at many stages in the process. "Up regulation" refers to regulation that increases the production of expression products (I.e., RNA or protein.), while "down regulation" refers to regulation that decreases production. Molecules (e.g., transcription factors) that are involved in up regulation or down regulation are often called "activators " and "repressors" respectively.

[0099] in addition to containing introns, genomic forms of a nucleic acid molecule may also include sequences located on both the 5' and 3' end of the sequences thai are present on an RNA transcript. These sequences are referred to as "flanking" sequences or regions (these flanking sequences are located 5' or 3' to the non-translated sequences present on the mRNA transcript). The 5' flanking region may contain regulatory sequences such as promoters and enhancers that control or influence the transcription of the gene. The 3' flanking region may contain sequences that direct the termination of transcription, post- transcriptional cleavage and polyadenyiation.

[Θ01Θ0Ι A nucleic acid molecule may be present in a cDNA, genomic DNA or RNA form. When present in a DNA form, the nucleic acid molecule may be single-stranded (i.e., the sense strand) or double-stranded. Suitable control elements such as enhancers/promoters, splice junctions, polyadeny iation signals, etc. may be placed in close proximity to the coding region of the nucleic acid molecule if needed to permit proper initiation of transcription and/or correct processing of the primary RNA transcript. Alternatively, the coding region utilized in expression vectors may contain endogenous enhancers/promoters, splice junctions, intervening sequences., polyadenyiation signals, etc. or a combination of both endogenous and exogenous control elements.

[001011 A nucleic acid molecule may produce multiple RNA species that are generated by differential splicing of the primary RN A transcri pt. cDN As that are splice variants of the same gene will contain regions of sequence identity or complete homology (representing the presence of the same exon or portion of the same exon on both cD As) and regions of complete non-identity (for example, representing the presence of exon "A" on cDNA 1 wherein cD A 2 contains exon "B" instead). Because the two cDNAs contain regions of sequence identity they will both hybridize to a probe derived from the entire nucleic acid molecule or portions of the nucleic acid molecule containing sequences found on both cDNAs; the two splice variants are therefore substantially homologous to such a probe and to each other.

[001.02] In certain embodiments the nucleic acid molecule is provided i the form of expression vecto or expression cassette.

[001.03] In certain embodiments the nucleic acid molecule may encode a polypeptide product which is expressed episomally, for example when the nucleic acid is presented in the form of vector, such as a plasmid. The use of a suitable extracliromosomal episomat vector within the cell nucleus provides a means of maintaining a polynucleotide sequence in target cells in high copy number extra-chromosomally, tliereby eliminating potential effects of chromosomal integration. In these embodiments, the sequence which encodes the polypeptide product may be associated with a promoter which facilitates transcription of the sequence which encodes the polypeptide product. The promoter may be a constitutive promoter or an inducible promoter. Suitable promoters may include, but are not necessarily limited to a cytomegalovirus (CMV) promoter (see, for example Chen, X., Frisina, R. D., Bowers, W. J., Frisina, D, R., Federoff. H. J. (2001) HSV amplicon-mediated neurotrophin-3 expression protects murine spiral ganglion neurons from cisp latin-induced damage. Molecular Therapy 3 (6): 958-63 or Kawamoto, ., Sha, S, ft, Minoda. R., Izumikawa, M., Kuriyama, H., Schacht, J., Raphael, Y. (2004) Antioxidant gene therapy can protect hearing and hair cells from ototoxicity. Molecular Therapy 9 (2): 173-181 , the entire contents of which are incorporated herein by reference). The promoter may comprise a Myosin Vila (Myo) or neuron specific enoiase (NSE) promoter, as these are cell specific transcription regulators for hair cells (Myo) and spiral ganglion neurons (NSE). The promoter may comprise a cytomegalovirus immediate early (IE) enhancer (CMV-IE), or a chicken beta- actiti promoter (CAG), or an elongation factor I alpha promoter EF-3 alpha) or a Rous sarcoma virus promoter (RS ^' V) (see, for example, Liu, Y., Okada, T., Nomoto, T., e, X., Kume, A., Ozawa, K., Xiao, S. (2007) Promoter effects of adeno-assoeiated viral vector for transgene expression in the cochlea in vivo. Exp Mol Med. 39 (2) 170-175, the entire contents of which are incorporated herein by reference). The promoter may comprise a glial fibrillary acidic protein (GFAP) promoter. The promoter may comprise a CBA promoter, which is a hybrid promoter comprising a sequence from the chicken β-aetin promoter and from the CMV promoter. [001.04] In certai embodiments the nucleic acid molecule comprises a post- transcriptional regulatory element, for example to enhance expression of the agent, such as the woodchuck hepatitis virus posttraiiscriptionai regulatory element (WP E) (see, for example Stone, I. M., Lurie, D. I., Kelley, M, W., Paulsen, D, J, (2005) Adeno-associated virus-mediated gene transfer to hair ceils and support cells of the murine cochlea. Mol Ther 1 1(6): 843-84 and Xu, L., et at (2001) CMV-beta-actin promoter directs higher expression from an adeno-associated viral vector in the liver than the cytomegalovirus or elongation factor 1 alpha promoter and results in therapeutic levels of human factor X in mice. Bum, Gene Ther. 12: 563-573, the entire contents of which are incorporated herein by reference) .

[00105] In certain embodiments the Nrg-ICD enhancer and/or PSD-95 promoter is used for targeting of the expression of agent in spiral ganglion neurons (see for example Bao, J., Lin, H., Ouyang, Y., Lei, D., Qsman, A., Kim, T. W., Met, L., Dai, P., Ghlerniiler, . ., Arabron, R. T. (2004) Activity-dependent transcription regulation of PSD-95 by neuregulin- 1 and Eos. Nature N roscience 7 (11): 1250-8, the entire contents of which is incorporated herein by reference).

[00106] In certain embodiments the nucleic acid molecule may be incorporated into the genome of cells within th target region,

[00107] In an embodiment, the polypeptide product which is encoded by the nucleic acid molecule may be a neurotrophic factor. In one embodiment the nucleic acid may encode a neurotrophic factor for spiral ganglion cells or cochlear hair cells. As used herein a "neurotrophic factor" is a polypeptide possessing at least one activity selected from promoting the growth and survival of developing neurons, maintaining/supporting mature neurons, and/or directing neural growth towards the source of the neurotrophic factor, and/or supporting the survival of spiral ganglion cells, and/or supporting the survival of cochlear hair ceils. According to an embodiment, the neurotrophic factor is a polypeptide possessing at least one activity selected from promoting or maintaining neurite outgrowth from spiral ganglion cells and/or directing neurite outgrowth from spiral ganglion cells towards the source of the neurotrophic factor, and/or supporting the survival of spiral ganglion cells, and/or supporting the survival of cochlear hair cells, in a particular embodiment the neurotrophic factor possesses activity in promoting or maintaining neurite outgrowth from spiral ganglion cells and/or directing neurite outgrowth from spiral ganglion cells towards the source of the neurotrophic factor, and/or supporting the survival of spiral ganglion cells. The neurotrophic factor may be encoded as a preprotein, and optionally subsequently processed to a mature form. In certain embodiments, the neurotrophic factor may be selected front Neuroteophin-3 (NT-3) or Neurotrophin-3 precursor molecule or a preprotein form thereof ( NCBI Accession No. HGNC:8Q23, Swiss Prot Accession Number P20783) or homologs or human variants thereof; Neurotrophin 4/5 (NT-4/5, NT-4, NT-5, NTF4, NTF5) or a preprotein form thereof ((HGNC:8024), Ensembl accession No, ENSP00O00328738, GenBank accession No, AAA60154, version GI: 190265) or homologs or human variants thereof; Nerve growth factor (NGF) (NCBI Accession No. NP_002497.2 version GI:7099531 ) (HGNC:7S08) _; Ensembl accession no. ENSP00000358525), preprotein forms thereof or homologs or human variants thereof; Brain derived neurotrophic factor (BDNF, Brain-derived neurotrophic factor Precursor, Abrineurin) (the preprotein form of which is described in NCBI Accession No. NP_001 137277.1 version 01:219842288, UmProtKB/Swiss-Prot Accession No P23560) or homologs or human variants thereof; glial cell line-derived neurotrophie factor (G.DNF, glial ceil derived neurotrophic factor precursor)(NCBI Accession No. HGNC:4232 (gene) or the preprotetn forms as described in NCBI Accession Numbers NP_00O505 and NP 000505. I ) or homologs or human variants thereof; ciliary neurotrophic factor (CNTF)(NCBI Accession No. NP_000605 version GI:4758020 (HGNC2169), UniProt B/ ^' Swiss-Prot Accession No, P26441) or homologs or human variants thereof; Activity dependent neurotrophic factor (ADNF, ADNP1; IAA0784; ADNP, or Activity-dependent neuiOprotector homeobox protein) (Ensembl Accession Numbers: EN5P00000360662, ENSP00000379346, ENSP00000342905, and ENSP00000379349) or homologs or human variants thereof, or f gments thereof, or the ADNF homologues ADNF-9 or the active peptide region with an amino acid sequence of SALL SIPA, or ADNF- 14 (neuropeptide) or the neuroprotective NAP peptide of ADNF with an amino acid sequence of NAPVSIPQ; the cytokine trophic factor leukaemia inhibitory factor (LIF) (NCBI accession No. HGNC:6596 (genomic sequence), UniProtKB/Swiss-Prot Accession No. NP 002300.1 version 01:4504991 (polypeptide), typically in conjunction with a neurotrophin such as NT-3; and Fibroblast growth factor (FGF 1, FGF 2) (NCBI accession No NM_002006 (genomic sequence), UniProt B/Swiss- Prot Accession No. P09038 (polypeptide)) or homologs or human variants thereof, which is believed to interact with NT3 and to support neurite outgrowth and growth cone development, or in a combination of FGF-2 & GDNF; macrophage migration inhibitory factor (MIF), a cytokine the promotes inner ear sensory neuritogenesis (Bank, LM, et al. (2012), "Macrophage migration inhibitory factor acts as a neurotrophin in the developing inner ear". Development 139(24):4666-74), e.g. human migration inhibitor '' factor (MIF), Genbank accession no. M25639; neural cell adhesion molecule (CAM) - LI provides guidance cues in the nervous system (Brummendorf T. and atiijen, F.G. (1996), "Structure/function relationships of axon -associated adhesion receptors of the immunoglobulin superiority", Cum Opin. NeurohioL 6: 584-593. PMID: 8937821 ) and its expression in the cochlea indicates a putative role in spiral ganglion neurite guidance (Hrynko S.H. el. al (1998), "Multiple roles of neural cell adhesion molecule, neural cell adhesion molecule-potysialic acid, and LI adhesion molecule during sensory innervation of the otic epithelium in. vitro", Neuroscience 87: 432-437.), e.g. human L1 CAM Genbank accession no. X59847.

[00108] The polypeptide product which is encoded may he an Apyrase (IUBMB Enzyme Nomenclature Enzyme classification EC 3.6.1.5), an enzyme which hydrolyses ATP extracellularly and therefore prevents P2X receptor activation which can inhibit neurotrophin-dependent neurite outgrowth. The polypeptide product may be an ectonucleotidase, an enzyme which promotes the production of adenosine, which is neuroprotective via adenosine receptors on spiral ganglion neurons. An example of a suitable ectonucleotidase in human subjects is ectonucleoside triphosphate diphosphohydrolase 2 (ENTPD2) (NCBI Accession no.. KM 001246).

[00109] The polypeptide product which is encoded by the nucleic acid molecule may be involved in the melanocortin signalling pathway, for example alpha-melanocyte stimulatin hormone (alpha-MSH). The tnelanocortins. make up a family of endogenous peptides derived from pro-opiomelanocortin, which bind to five melanocortin receptors (MC s). Other examples of melanocortins which may be encoded by the nucleic acid molecule include proopiomelanocortin (POMC) or its cleavage products (adrenocorticotropin/ beta- iipotrophv alpha-melanocyte stimulating hormone/ ^' beta-melanocyte stimulatin hormone/ beta-endorphin) or precursors of active peptides (for gene NCBI Accession ^' No. HG ^' NC:920l; for precursor NCBI accession No AAA60140 version GI:190188).

[001.10] The polypeptide product which is encoded by the nucleic acid molecule may be a morphogen. for example a bone morphogenic protein (BMP), Sonic hedgehog, or Wingless/Wnts.

[00111] The polypeptide product which is encoded by the nucleic acid molecule may be an axon guidance factor which is capable of attracting neurites, such as spiral ganglion cell neurites, such as laminin, Netrin-1 , an Ephrin, a semaphorin, a Slit such as a 3 slit homolog, slit2 or siit3 or a cytokine such as Leukemia inhibitory factor. (b) Nucleic acid molecules which modulate endogenous gene expression

[00112| In certain embodiments the agent may comprise or consist of a nucleic acid molecule which modulates the expression of an endogenous gene sequence. Depending on the particular application, the modulation of expression may up-regulate or down-regulate expression of the gene. The modulation of expression of the endogenous gene may be a direct regulation of expression, for example by directly modulating transcription or translation of an endogenous gene. The modulation of expression of the endogenous gene by the nucleic acid molecule may be an indirect regulation of expression, for exampl by expression of a polypeptide by the nucleic acid molecule which in turn modulates the expression of the endogenous gene. An example may be targeted inhibition of adenosine kinase (NCBl accession No HGNC:257 (genomic sequence); NP_O01 1 14.2 (polypeptide)), leading to increased extracellular adenosine levels which are neuroprotective.

[Ό0113] The modulation of expression of an endogenous gene sequence may be an up- regulation of an endogenous gene sequence. For example, the nucleic acid molecule may encode a transcription factor which promotes the expression of an endogenous gene, such as a gene which encodes a neurotrophic factor. The Sox2. Bra-3.1 and Gata 3 transcription factors are examples of this class of cell signalling molecule associated with determination of sensory hair cell, supporting cell and spiral ganglion neuron development and survival in the cochlea ( eith!ey, EM, Erkman, L., Bennett, T., Lou, L. ^" Ryan, AJF. (1.999) Effects of a hair cell transcription factor Brn-3.1 , gene deletion on homozygous and heterozygous mouse cochleae in adulthood and aging. Hearing Research 134:71 -76; Van der Wees, J., Van Looij, M.A., de Riuter, MM, Elias, H., van der Burg, H,, Liem. S.S., Kurek, D., Engle, J.D.. Karis, A., van Zanten, B.G., de Zeeuw, C.L, Grosveld, F.G., van Doornick, J.H. (2004) Hearing loss following Gata3 hapiomsufficiency is caused by cochlear disorder. Ne rahiol Bis 16:169-78; Dabdoub, A., Puligilla, C, Jones, J.M., Fritzsch, B., Cheah, K.S., Pevney, L.H. Keltey, M.W. (2008) Sox2 signalling in prosensory domain specification and subsequent hair cell differentiation in the developing cochlea. Proc Natl Acad Set USA 105: 18396-401; and Appier, J., Koundakjian, E., Lu, C, Goodrich, L. (2009) Gata3 regulates neurite extension and targeting of spiral ganglion neurons. Proc, 7th Molecular Biology of Hearing and Deafness conference. June 20-23, Harvard Medical School, Boston. MA, USA.).

[00114] The Adenovirus-mediated transduction o the transcription factor atonal homologue 1 (Atohl, also known as Math I, ΑΤΉ1 , HATHl or bHLHal4) (NCBl accession No. BC069578 version GT.47480322 (HGNC:797), Uniprot accession Nos. Q92858; Q 14CT9) into mouse or guinea pig cochlear cells has been described to lead to the generation of a cochlear hair cell-like phenotype from supporting cells, which led to secretion of trophic factors, the attraction of neurite outgrowth and the forniation of functional synapses between the transformed cells and auditory spiral ganglion cells. Accordingly, the methods described herein contemplate the transfection of the transcription factor atonal homologue 1 into cochlear cells using eleetroporation, without the need for viral vector-mediated delivery.

[00115] Other transcription factors which modulate the expression of neurotrophic factors in ceils are known in the art. For example, the transcription factor SRY-box- containing gene 10 (Sox 10) induces a greater than 100 fold u regulation of the expression of ciliary neurotrophic factor in Schwann cells in the mouse, whilst knockdown of Sox 10 results in a reduction of expression ciliary neurotrophic factor by more than 80%. Accordingly, the methods described herein contemplate the transfection of cells within a target region in a subject by eleetroporation of a nucleic acid molecule encoding a transcription factor, wherein the transcription factor modulates the expression of an endogenous neurotrophic factor, or wherein the transcription factor modulates the expression of an exogenous polynucleotide sequence which encodes a neurotrophic factor and which is transfected with the transcription factor.

[00116] The up-regulation of a gene sequence may be achieved by the use of gene targeting constructs to generate transcription units formed by homologous recombination between an. endogenous target gene and the targeting construct. In such methods, expression of a desired targeted gene in a cell (i.e., a desired endogenous cellular gene) is altered by the introduction by homologous recombination into the cellular genome at a preselected site, of DMA which includes at least a regulatory sequence, an exon and a splice donor site. These components are introduced into the chromosomal (genomic) DNA in such a manner that this, in effect, results in production of a new transcription unit (in which the regulatory sequence, the exon and the splice donor site present in the DN A construct are operatively linked to the endogenous gene). As a result of introduction of these components into the chromosomal DN A, the expression of the desired endogenous gene is altered.

[00117} The production and use of targeting constructs are described, for example, in US patent No 5,641 ,670 (Treco e( al.), the entire contents of which are incorporated herein by reference. Endogenous genes which may be up-regulated by these techniques include neurotrophic factor genes selected from NeUfOtfOphin-3 (NT-3) or Neurotrophm-3 precursor molecule or a preprotein form thereof (NCBi Accession No. HGNC: 8023, Swiss Prof Accession Number P20783) or homologs or human variants thereof; Neurotrophin 4/5 (NT- 4/5, NT-4, ΝΓ-5, NTF4, NTF5) or a preprotein form thereof ((HGNC:8024), Ensemhl accession No. ENSP00000328738, GenBank accession No. AAA60154, version Gl: 190265} or homologs or human variants thereof; Nerve growth factor (NGF) (NCBI Accession No. NP_ 002497.2 versio GI:709953I ) (HGNC:7808), Ensembl accession no, ENSP00000358525). preprotein forms thereof or homologs or human variants thereof; Brain derived neurotrophic factor (BDNF, Brain-derived neurotrophic factor Precursor, Abrineurin) (the preprotein form of which is described in NCBI Accession No. NP_00I 137277.1 version 01:219842288, UniProtKB Swiss-Prot Accession No P23560) or homologs or human variants thereof; glial cell line-derived neurotrophic factor (GDNF, glial cell derived neurotrophic factor precursor)(NCBI Accession No. HGNC:4232 (gene) or the preprotein forms as described in NCBI Accession Numbers NP 000505 and NP 000505.1) or homologs or huma variants thereof; ciliary neurotrophic factor (CNTF)(NCBI Accession No. NP_000605 version GI:4758020 (HGNC;2169), UniProtKB/Swiss-Prot Accession No. P26443) or homologs or human variants thereof; Activity dependent neurotrophic factor (ADNF, ADNP1 ; K1AA0784; ADNP, or Activity-dependent neuroprotector homeobox protein) (Ensembl Accession Numbers: ENSP0O00Q360662, ENSP00000379346, ENSP00Q0034290S, and EN SP00000379349) or homologs or human variants thereof, or fragments thereof, or the ADNF homologues ADNF-9 or die active peptide region with an amino acid sequence of SALLRSIPA, or ADNF- 14 (neuropeptide) or the neuroprotective NAP peptide of ADNF with an amino acid sequence of NAPVSIPQ; the cytokine trophic factor leukaemia inhibitory factor (LTF) (NCBI accession No. HGNC:6596 (genomic sequence), UniProtKB/Swiss-Prot Accession No. NP_002300J version GI;4504991 (polypeptide), typically in conjunction with a neurotrophin such as NT-3; and Fibroblast growth factor (EOF 1, FGF 2) (NCBI accession No NM 00200 (genomic sequence), UniProtKB/Swiss-Prot Accession No. P09038 (polypeptide)) or homologs or human variants thereof, which is believed to interact with NTS and to support neurite outgrowth and growth cone development, or in a combination of FGF-2 & GDNF; Apyrase; or eetonucleoside triphosphate diphosphohydrolase 2 (ENTPD2) (NCBI Accession no. NM 001246); a gene encoding a polypeptide involved in the melanocortin signalling pathway, for example alpha- melanocyte stimulating hormone (alpha-MSH), proopiomelanocortin (POMC) (HGNC Accession No. HGNC: 201 ), or a gene encoding a morphogen, for example a bone morphogenetic protein (BMP), Sonic hedgehog, or Wmgless/Wnts, a gene encoding an axon guidance factor, such as iar nin, Netrin-1, an Ephrin, a semaphorin, a Slit such as a 3 slit homolog, slif2 or slit3 or a cytokine such as Leukaemia inhibitory factor; a gene encoding macrophage migration inhibitory factor (MIF), a cytokine the promotes inner ear sensory neuntogenesis (Bank, LM, et al (2012)., "Macrophage migration inhibitory factor acts as a neurotrophin in the developing inner ear", Development l39(24):4666-74), e.g. human migration inhibitory factor (MIF), Genbank accession no. M2563 ; a gene encoding macrophage migration inhibitory factor (MIF), a cytokine the promotes inner ear sensory neural cell adhesion molecule (CAM) - LI provides guidance cues in the nervous system (Briimmendorf T. and Rathjen, F.G. (1996), "Structure function relationships of axon- associated adhesion receptors of the immunoglobulin snperfamily", Curr. Opin. Ne robiol 6: 584-593. PMFD: 8937821 ; Hrynko S.H. et al (1998), "Multiple roles of neural cell adhesion molecule, neural cell adhesion molecuie-polysialic acid, and LI adhesion molecule during sensory innervation of the otic epithelium in vitro", Neurosclence 87: 432-437.), e.g. human LI CAM Genbank accession no. X59847.

[00118] The modulation of expression of the endogenous gene may be the down- regulation of expression of an endogenous gene. The down-regulation of expression of the endogenous gene may, for example, be the down-regulation of a transcription factor which constittitively or inducibly inhibits the expression of a neurotrophic factor by a cell within a target region in a subject, such as a cochlear mesenchymal cell, which results in the cell expressing a neurotrophic factor.

[00119] The down-regulation of an endogenous gene may utilize the targeted disruption of the expression of one or more endogenous genes using any nucleic acid molecules which selectively target and inhibit the expression of the genes, such as antisense sequences, varieties of small interfering RNA (siRNA) sequences. shRNA sequences, mieroRNAs. ribozyme sequences, and the like. The "expression" of endogenous genes is intended to encompass the transcription and/or translation of endogenous gene sequences.

[00120J Methods for the design, synthesis, and delivery of antisense nucleic acid molecules are well known in the art. The antisense molecules may be DNA or RNA, or partial or complete synthetic analogues thereof. Sequences of/for antisense constructs may he derived from various regions of the target gene(s). Antisense constructs may also be designed to target and bind to regulatory regions of the nucleotide sequence, such as the promoter., or to coding (exon) or non-coding (intron) sequences. Antisense polynucleotides of the invention may be generated which are at least substantially complementary along their length to the region of the gene in question. Binding of an antisense polynucleotide to its complementar cellular sequence may interfere with transcription, RNA processing, transport, translation and/or mRNA. stability. [00121] Suitable antisense oligonucleotides may be prepared by methods well known to those of skill in the ait. Typically antisense oligonucleotides will be synthesized on automated synthesizers. Suitable antisense oligonucleotides may include modifications designed to improve their delivery into cells, their stability once inside a cell, and or their bindin to the appropriate target. For example, the antisense oligonucleotide may be modified by the addi tion of one or more phosphorothioate linkages, or the inclusio of one or more morpholine rings into the backbone. The antisense oligonucleotide may be 10-30 base pairs in length.

[00122] As a practical matter, the level of complementarity between the antisense nucleic acid molecule and the target mRNA or cDNA sequence, or portion thereof can be determined- conventionally using known computer programs such as the Bestfit program (Wisconsi Sequence Analysis Package., Version 8 for Unix, Genetics Computer Group. University Research Park, 575 Science Drive, Madison, Wis. 53711), Bestfit uses the local homology algorithm of Smith and Waterman to find the best segment of homology between two sequences (Advances in Applied Mathematics 2:482-489 (1981 )). When using Bestfit or any other sequence alignment program to determine whether a particular sequence is, for instance, 90% identical to a reference sequence, the parameters are set such that the percentage of identity is calculated over the full length of the reference nucleotide sequence and that gaps in homology of up to 5% of the total number of nucleotides in the reference sequence are allowed. A preferred method for determining the best overall match between a query sequence and a subject sequence, also referred to as a global sequence alignment, can be determined using the FASTDB computer program based on the algorithm of Brutlag and colleagues (Comp. App. Biosci. 6:237-245 ( 1990)). In a sequence alignment the query and subject sequences are both DNA sequences. An RNA sequence can be compared by converting U's to T's. Hie result of said global sequence alignment is in percent identity. Preferred parameters used in a FASTDB alignment of DNA sequences to calculate percent identity are: Mati'ix=lJnitary, k-tuple=4, Mismatch Penalty^! , joining Penalty=30, Randomization Group Length=0, Cut-off Score=l , Gap Penalty=5, Gap Size Penalty 0.05, Window Size=500 or the length of the subject nucleotide sequence, whichever is shorter.

[00123] If tire subject sequence is shorter than the query sequence because of 5' or 3' deletions, not because of internal deletions, a manual correction must be made to the results. This is because the FASTDB program does not account for 5' and 3' truncations of the subject sequence when calculating percent identity. For subject sequences truncated at the 5' or 3 ^r ends, relative to the query sequence, the percent identity is corrected by calculating the number of bases of the query sequence that are 5' and 3 ' of the subject sequence, wh ich are not matched/aligned, as a percent of the total bases of the query sequence. Whether a nucleotide is matched/aligned is determined by results of the FASTDB sequence alignment This percentage is then subtracted from the percent identity, calculated by the above FASTDB program using the specified, parameters, to arrive at a final percent identity score. Only bases outside the 5' and 3' bases of the subject sequence, as displayed by the FASTDB alignment, which are not matched/aligned with the query sequence, are calculated for the purposes of manually adjusting the percent identity score.

[00124| Small interfering RNA (siRNA) sequences are small, usually double-stranded RNA oligonucleotides, for example at 1 , 21 , 27 or 29 bases in length, with or without overhangs, which specifically hybridize with RNA sequences of interest and which serve as substrates for the R A-induced silencing complex. Double-stranded RNA molecules may be synthesized in which one strand is identical to a specific region of the mRNA transcript to be silenced, and this double stranded RNA may be introduced directly. Alternatively, corresponding dsDNA can be employed, which, once presented intracelluiar!y is converted into dsRNA. Methods for the design and synthesis of suitable siRNA molecules for use in RNA interference (RNAi) and for achieving post-transcriptionai gene silencing are well known to those of skill in the art. For example, rules for the rational design of siRNA are available online in "Rules of siRNA design for RNA interference (RNAi)" at <btt.p:// ^' www.pro^

^• interference— NAi-.-3210.bttnl>; These rational design principals are also described in Elbashir SM ei al. (2001), "Duplexes of 21 -nucleotide RNAs mediate RNA interference in cultured mammalian cells", Nature. 411:494-498; Elbashir SM ei ai. (2001). Functional anatomy of siRNAs for mediating efficient RNAi in Drosophila me!tmogasier embryo lysate. EMBO J, 20:6877-6888; Elbashir SM ei al. (2002), "Analysis of gene function in somatic mammali n cells using small interfering RNAs", Methods, 26: 199-213; Reynolds A, Leake D, Boese Q, Scaring© S, Marshall WS, Khvorova A (2004), "Rational siRNA design for RNA interference", Nat Biotechnol. 22(3):326-30; and online at The entire contents of each of these publications is incorporated herein by reference.

[00125] Examples of two siRNA design tools which implement the rational siRNA principals discussed above are design algorithms offered by Dharmacon, Inc and a downloadable Microsoft Excel™ template, written by Maurice Ho at <http ;//boz094. ust hk/R Ai/si RN A>. [Θ012ό| The skilled addressee will appreciate that a range of suitable siRNA or nucleic acid -molecules comprising an siRNA sequence which is capable of inhibiting, the expression of an endogenous gene can be identified and generated based on knowledge of the sequence of the genes in question using routine procedures known to those skilled in the art without undue experimentation. Those skilled i the art will appreciate that there need not necessarily be 100% nucleotide sequence match between the target sequence and the siRNA sequence. The capacity for mismatch is dependent largely on the location of the mismatch within the sequences. In some instances, mismatches of 2 or 3 nucleotides may be acceptable but in other instances a single nucleotide mismatch is enough to negate the effectiveness of the siRNA. The suitability of a particular siRNA molecule may be determined using routine procedures known to those skilled in the art without undue experimentation.

[00127] Although the maximal effects of atitisense nucleic acids and siRNA on the specific inhibition of RNA or protein expression are comparable, siRNA generally produces a longer-lasting effect.

[Θ012&] Ribozymes, such as hammerhead or hairpin ribozymes, are capable of the targeted catalytic cleavage and spiicin of specific RNA sequences, including inRNA and genomic RNA sequences. The design and methods for the delivery of ribozymes are reviewed, for example, in Vaish, ore and Eckstein (1998) Nucleic Acids Research 26:5237- 5242; in Lieber and Strauss (1995) MoL Cell. Biol. 15:540-551 ; and in Usman and Blatt (2000) J Clin Invest 106:1197-1202, the entire contents of each of which are incorporated herein by reference.

[G0129| Short hairpin RNA (shRNA) is a sequence of RNA with a tight hairpin turn structure which is introduced into ceils as part of a vector which comprises a constitutive promoter such as a U6 promoter to allo shRNA to be constitutively expressed. The shRNA hairpin structure is cleaved by the cellular machinery into siRNA, which is then bound to the RNA-induced silencing complex (RISC), This complex binds to and cleaves mRNAs which match the siR A that is bound to it. The vector comprising the shRNA sequence is usually passed on to daughter cells in cell division, allowing the gene silencing to be inherited. Strategies- for designing shRNA sequences and nucleic acid molecules comprising shRNA for use in mammalian cells are known, for example clntyre and Fanning, Design and cloning strategies for constructing shRNA expression vectors, BMC Biotechnol. (2006) 6: 1.

[001301 The nucleic acid may be microRNA. The term microRNA or "mtRNA" as used herein has the ordinary and plain meaning of a non-coding RNA molecule found in eukaryotes which is involved in RNA -based gene regulation. The term can he used to refer to the single-stranded RNA molecule processed from a precursor or in certain instances the precursor itself or a mimetic or inhibitor thereof. MicroRNAs are capable of control of expression of genes in humans and non-human animals, and pla a significant role in control of development regulatory mechanisms (see, for example, John B, et at (2004), "Human MicroRNA Targets", PLoS Biol 2(11): e363. doi: 10..1371/jaurnal.pbio.0020363; and Li et al (2010), "Computational approaches for microRNA studies: a review", Mamm Genom.HQ- 2): 1-12). The involvement of microRNA in normal cochlear function and in a familial cochlear disorder has also been described in Meneia et al. Nature Genetics (2009) 4 f (5):609-613. It is anticipated that the modulatio of microRNA activity in cells of the cochlea, either by the introduction of microRNA or microRNA mimics into cells or the silencing of endogenous microRNA with a microRNA inhibitor/decoy (see, for example, Muliokandov et al (2012), '"High-throughput assessment of microRNA activity and function using microRNA sensor and decoy libraries", Nat. Methods 9(8): 840-846) may provid an approach for modulating the expression of particular targeted genes. Strategies for the administration of microRNA into cells or tissues of subjects have been described in International patent publication No. WO 2008/073920, which describes methods of modulating gene expression, or biologic or physiologic pathways in a cell, a tissue, or a subject comprising administering to the cell, tissue, or subject an amount of an isolated nucleic acid or mimetic thereof comprising a specific microRN A or microRNA inhibitor nucleic acid sequence in an amount sufficient to modulate the expression of a gene positively or negatively modulated by the microRNA, Studies have demonstrated therapeutic application of modulating microRNA in animals, including primates,

[00131] Techniques for the design of microRNA and microRNA inhibitors are readily available. A database of known microRNA sequences, new microRNA sequences and predicted microRN A targets is maintained at miRBase <http://microrna.sanger.ae. uk/> and described in "miRBase: tools for microRNA genomics" Griffiths-Jones S, Saint HK, van Dongen S, Enright Al. NAR 2008 36(Database issue):D 154-0158; "miRBase: microRNA sequences, targets ^' and gene nomenclature" Griffiths -Jones S, Gr ^' ocock RJ, van Dongen S, Bateman A, Enright AL NAR 2006 34(Database lssue):D140-D144; and "The microRNA Registry." Griffiths- J ones S. NAR 2004 32(Database Issue):D109-Dl 1 1. Commercial services are available for the design and synthesis of specific microRNA inhibitors based on a provided target sequence. [00132] Alternatively, avoidance of roicroRNA suppression of a target gene may be achieved by introducing into cells in a target region in a subject, as described above, an exogenous homologous sequence sufficiently different to the endogenous target sequence such that the rnicroRNA is insufficiently homologous to the introduced sequence to achieve suppression.

[00133] Expression of genes within target regions may also be regulated by introduction of mutations.

[00134] Mutations may be inserted into target genes by an method as known in the art,

[00135] For example, an exogenous nucleic acid may be introduced which comprises an oligonucleotide or polynucleotide which introduces a mutation comprising single or multiple nucleotide insertions, deletions or substitutions into the endogenous target gene.

[00136] Single o multiple nucleotide insertions, deletions or substitutions may be introduced via recombination of the target mutation site with an introduced targeting nucleotide sequence. Such an introduced nucleotide sequence may, for example, comprise a nucleotide sequence to be introduced into the genome flanked either side by nucleotide sequences homologous to target sequences contiguous in or located either side of a desired mutation insertion point. I accordance with the methods of the present invention, a nucleotide sequence to be introduced into the genome may also include a selectable marker operably linked to desired regulatory regions (which may include, for example, a stress - inducible promoter).

[00137] The nucleotide sequences homologous to the target sequences may be isogenic with the target sequences to thereby promote the frequency of homologous recombination.

[00138] Homologous nucleotide sequences that are not strictly isogenic to the target sequences can also be used. Although mismatches between the homologous nucleotide sequences and the target sequences can adversely affect the frequency of homologous recombination, isogenieity is not strictly required and substantial homology may be sufficient. For the purposes of the present invention, the level of homology between the homologous sequences and the target sequences may be at least about 90% identity, at least about 95% identity, at least about 99% identity or 100% identity.

[00139] A targeting nucleotide sequence can be comprised in a vector. Representative vectors include plastnids. cosinids, and viral vectors. Vectors can also comprise nucleic acids including expression control elements, such as transcription translation control signals, origins of replication, po!yadenyiation signals, internal ribosome entry sites, promoters. enhancers, etc., wherein the control elements are operatively associated with a nucleic acid encoding a gene product. Selection of these and other commo vector elements are conventional and many such sequences can be derived from commercially available vectors. See, for example, Sambrook, J. et al, Molecular Cloning, Cold Spring Harbor Laboratory (1.989), Sambrook, J. and Russell, IX W. (2001), "Molecular Cloning: A laboratory Manual", 3rd edition, Cold Spring Harbor Laboratory Press, and references cited therein and Ausubel et al. (eds) Current Protocols in Molecular Biology, Joh Wiley & Sons (2000).

[00140] Optionally, a targeting DNA is co-administered with a recombinase, for example recA, to a target cell to thereby enhance the rate of homologous recombination. The target cell(s) may already comprise, or have been transformed to comprise suitable recombinase target sequences, if required. For example, a recombinase protein(s) can be loaded onto a targeting DNA as described in U.S. Pat. No. 6,255,1 13. To enhance the loading process, a targeting DNA can contain one or more reeornbinogenic nucieation sequences. targeting DNA can also be coated with a recombinase protei by pre -incubating the targeting polynucleotide with a recombinase, whereby the recombinase is non-covalently bound to the polynucleotide. See, for example, A. Vergunst et al (1998), Nucleic Acids Res. 26:2729 and A. Vergunst and P. Hooykaas (1998), Plant Motec. Biol. 38:393 406, International patent publications WO 99/25821 , WO 99/25840, WO 99/25855, and WO 99/25854 and U.S. Pat. Nos. 5,780,296, 6,255,1 13, and 6,686,515.

[00141 Mutations may also be introduced into plants using zinc finger nucleases (ZFNs), transcription activator-like effector nucleases (TALENs), clustered regulatory interspaced short palindromic repeat (CRISPR)/ Cas-based RKA-guided DNA en donuei eases, and homing endonucleases (HEs) as discussed in, for example, Gaj T. et al (2013; Trends Biotechnol 31(7): 397-405), Carroll D. (2012: Molecular therapy 20(9): 1659-1660), Xiao A. et al (6 June 2013; Nucleic Acids Research 2013, 1-1 L doi: 10.1093/nar/gkt464, P. Mali, K. M. Esveit, G. M. Church (2013), Nat. Methods 10, 957-96 (2013). doi: 10. l038/nraeth.2649; pmid: 24076990, and P. Mali et al., (2013), Science 339, 823-826 (2013). doi; 10. 126/science.1232033; pmid: 23287722, the disclosures of these references being hereby incorporated by cross-reference.

Agents other than nucleic a id molecules

[001.42] Also contemplated in the methods described herein is the eiectroporation of polypeptides, such as small neuroprotective polypeptides which may support the survival of cochlear hair cells and/or spiral ganglion cells. Examples of such molecules include the neuroprotective NAP peptide, activity dependent neurotrophic factor, FN peptide, ACTH ₄ .9 analogue Org 2766, or molecules comprising these peptides which retain neuroprotective activity in the cochlea.

Delivery of agents

[Θ01431 Agents for electroporation into one or more cells within a target region in a subject may be delivered to the target region by any suitable method as known in the art. Non-limiting examples may include injection or micromjection or coating of the agent directly on the rnicroarray.

[00144) in one embodiment, one or more of the electroporation electrodes, or the electroporation assembly comprising the electrode(s) or electrode array(s) may comprise a lumen, channel or groove which carries the agent and allows the delivery of the agent to the target region, or it may be associated with a separate catheter or cannula, such as a silicon or polymer tube which is attached to the electrode. Alternatively, the agent may be introduced into the target region via a cannula which may be inserted or removed independently to the electropofation electrode.

[00145] In embodiments in which a cochlear implant provides at least one electroporation electrode, the agent may be provided via a lumen, channel or groove in the cochlear implant electrode microarray. These forms of electrode may allow multiple rounds of introduction of agent into the cochlea following implantation of the cochlear implant and therefor multiple rounds of electroporation. The use of a cochlear implant with a lumen to deliver agents to the cochlea is described, for example, in Paasehe el al.„ (2003) Otology & Neurology 24:222- 227. the entire contents of which is incorporated herein by reference.

[00146] The lumen of the cochlear implant may be sealed following delivery of the agent.

[00147] US patent application publication No. 2008/0214986, th entire content of which is incorporated herein by reference, describes the use of gel compositions with a predetermined conductivity for use in optimising the electroporation of tissues. In certain embodiments, the electrodes and or cochlear implant may be coated with gel, as described in US 2008/0214986, to provide a degree of control over the conductivity of the volume surrounding the electrodes.

[00148| In certain embodiments of the methods described herein wherein t re cochlear implant comprises at least one electrode which is used for electroporation, the agent is delivered to the target region using a catheter or cannula which is not associated with the cochlear implant. In these embodiments typically the catheter or cannula is inserted into the target region and the solution comprising the agent is delivered. The catheter or cannula is then withdrawn, the cochlear implant is introduced and electroporation takes place, as described above.

|00l49| The agent may be delivered to the target region at the time the electroporation eiectrode(s) is/are inserted into the target region, or before the insertion of the electroporation electrode or after the insertion of the electroporation electrode. The agent may be provided in a diffusible form, such as incorporated in or associated with a biodegradable or biocompatible viscous liquid or gel solution surrounding the electrode array of the cochlear implant. The viscous liquid or gel solution may comprise polyacrylk acid (PAA ^' h polyvinyl alcohol (PVA), polylactic acid (PLA) and/or polyglycolic acid (PGA). Pluronic F127 (BASF) at < 30% solution may also be used to stabilise the media containing the agent. This approach may provide an advantage in that the agent is presented to the target region with the electrode(s) in situ in a stable concentration of at the rime of electroporation.

[00150] Where the agent comprises a nucleic acid molecule the agent may be presented in a diluent solutio which comprises ions and proteins, or the agent may be diluted in sterile distilled deionised. water, provided that the diluent solution does not substantially adversely affect the efficiency of electroporation or efficacy of the agent. Typically water is used as the diluent, or wit a generic buffer such as TRIS (50 mM), or isotonic saline, with the nucleic acid molecule provided typically at a concentration of from lOug μΙ to iOtig/j . The nucleic acid molecule may be provided within a buffered solution, such as phosphate- buffered saline (PBS; e.g. pH 7.4), or a perilymph-like solution (for example as described by Salt, A.N. Konishi, T. (1986) The cochlear fluids: Perilymph and endoSympk Eds. Altschuler, .A., Hoffman, D.W., Bobbin, R.P. In: Neurobiology of Hearing: The Cochlea. New York, Raven Press, pp. 109 - 122), and may be in the presence of divalent cation chelators such as ethylenediammetetraacetic acid (EDTA) for stabilization of the molecules.

|0O15l j Where the target region is located in a cochlea, the target region may be in the cochlear chamber, such as region comprising mesenchymal cells of the seal a tympani, of the scala vestibuli or of the scala media. In such an embodiment, typically the solution comprising agent is delivered to the base of the cochlea, typically via a perforated round window, so that the solution comprising the agent flows through the cochlear- perilymphatic chambers and displaces the media lying within the cochlea chamber. Alternatively the solution comprising the agent may be delivered via a eoehleostomy. The volume of the human scala tympani is in the order of 30μ1, the scala vestibuli 22 ΐ. and the scala media 2μ1. In order to ensure that the chamber contains sufficient agent the nominal volume of solution comprising the agent to be delivered to the cochlea may be in the order of 60μΙ or less. Typically, the controlled delivery of such a volume may be achieved through the use of a mechanical infusion micropiimp, or a micro syringe. Once the agent and electrode are present within the appropriate chamber(s) electroporation takes place, and in certain embodiments the electroporation electrode is then removed. In these embodiments of the invention the electroporation electrode may be withdrawn from the cochlea as a separate action to the placement of the cochlear implant, and typically the electrode is removed before the insertion of the cochlear implant.

[00152 J Preferred forms of the present invention will now be described, by way of example only, with reference to the followin examples, including comparative data, and w ^; hich are not to be taken to be limiting to the scope or spirit of the invention in an way .

Examples

Example I - Materials and Methods

Vector preparation

[00153 j The brain derived neurotrophic factor (BDNF) gene therapy cassette (CMVp- 3xflag-BDNF-IRES-hrGFP gene cassette) comprised a CMV (cytomegalovirus) promoter upstream of a C -terminal FLAG-tagged proBDNF gene (including signal peptide), with GFP co-expression afforded by an internal ribosome entry site (IRES) (see Figure la). Plasmid DMA was purified from E, eoli cultures using PureLink MaxiPrep kit (Invitrogen). DNA was resuspended in tris-buffered saline at 2 §/μί and stored at -20°C DNA was quantified on a anoDrop ND-1000 spectrophotometer (Thermo Scientific) and confirmed by gel electrophoresis. 20pL of the DNA suspension was used to transfect each cochlea via microinjection into scala tympani of the cochlea via the round window, followed by cochlear implant array-based electro potation.

Animal use

[00154] Coloured guinea pigs of either sex weighing between 500g and 7G0g were used for the procedures. Procedures were approved by the University of New South Wales Animal Care and Ethics Committee. Anaesthesia, was induced using 4% isoflurane delivered with oxygen, and maintained at 1-2% isoflurane. Buprenorphine ( 15pg kg im., Temgesic, Reckitt Benckiser), and medetomidine hydrochloride (175pg/kg i.tn.. Doraitor, Pftzer Animal Health) were used for analgesia and emofloxacin (5mg/kg i.m.. Baytril, Bayer Australia Ltd) was used as a prophylactic antibiotic. Body temperature control and cardio- pulmonary monitoring (<¾ saturation and heart rate) were sustained (MouseStat, Kent Scientific). Guinea pigs were euthanized via intraperitoneal, injection of pentobarbital (lOOmg/kg of body weight) at the conclusion of experiments, or to provide cochlear tissue for ex vivo close-field eiectroporation.

Microarray-based close-field eiectroporation ex vivo and organotypic culture

[00155| Both cochleae were removed immediately after euthanasia and placed into DMEM media (GIBCG) at room temperature. Under a dissection microscope a perforation was made through the round window using a 29 gauge needle and cochleae were placed onto sterile cloth dampened with Tris buffered saline (TBS). 2QpL of plasmid DNA was injected into scala tympani via the round window and the cochlear implant electrode array (part no. Z6027 L Cochlear Ltd, Australia) was then inserted into the basal turn (Fig. 1 c. d). Eiectroporation of the mesenchymal cells lining the scalae was achieved using between 5 and 50 pulses (50ms; 950ms interval ^' ) at varying voltages (CUY 21 square wave electroporator; NEPA GENE) in either a 'tandem' or 'alternating' electrode array configuration (Fig. Id). The resistance of the tandem and alternating array configurations ranged between 150 and 1200 ohms. After a 10 minute interval, the array was removed and the cochlea was incubated at room temperature for a further 20 minutes in the humidity chamber, before resection to enable organotypic culture. Dissected elements of the cochlea were placed into supplemented DMEM media (GIBCO), (10% fetal bovine serum and 100 umts/mL streptomycin and penicillin), and incubated at 37°C with 5% C0 ₂ for three to four days. Tissue was then fixed in 4% paraformaldahyde (PFA) for 30 minutes, then transferred to 0.1 M phosphate buffered saline (PBS), The number and location of GFP expressing cells on each .fragment of the cochlea was recorded using epifluorescence microscopy, Immuno- labelling was performed as described below on some cochleae to confirm expression of the FLAG-tagged BDNF neurotrophin gene construct (Fig. 7d; Fig. 9d). in vivo close-field eiectroporation gene delivery

[001561 In vivo eiectroporation of the BDNF gene construct was achieved using the electrode array inserted into scala tympani via the round window under anaesthesia. The surgical approach utilised a post-auricular exposure of the mastoid process. A hole was drilled through the bulla allowing visualisation of the round window. A. small perforation was made in the round window using a 29 gauge needle. 20pL of DNA solution was loaded into 0.96mm outside diameter, 0.58mm internal diameter PYC tubing, connected to a microperfusion pump. Fine forceps were used to match the end of the tubing to the face of the round window and the DNA was delivered into the cochlea. The electrode array was then inserted into scala tympani and close-field electroporation carried out as described for the m vitro experiments. Ten minutes after electroporation the array was removed and the wound, sutured and the animals supported during recovery. Analysis of mesenchymal cell transfection, recombinant BDNF production and neural regeneration was undertaken up to three weeks post-surgery by euthanizing the guinea pigs and perfusing the dissected cochlea with PFA as described above.

BDNF gene therapy in a deafened guinea pig model

[Θ0157| To study spiral ganglion regeneration with CFE-mediated neurotrophin gene therapy, guinea pigs were deafened using an ototoxic treatment targeting the sensory hair cells through eo-adminisuation of furosemide (IQOmg/kg i.v. via external jugular caniiulat on; Troy Laboratories), and i.m. injection of kanamycin sulphate (400mg/kg; Sigma), as previously described (Nelson, D. i., et at (2005), "The global burden of occupational noise-induced hearing loss". Am J Ind Med 48, 446-458). Deafness was confirmed using auditory brainstem response (ABR) and distortion product otoacoustic emission (DPOAE) measurement via a TDT systems III evoked potential workstation, including a Medusa optically-coupled headstage (Tucker-Davis Technologies) within a sound attenuating chamber (Sonora Technology) (after Holt, J. R. and Vandenberghe. L. H. (2012), "Gene therapy for deaf mice goes viral", Mo I Ther 20, 1836-1837). Testing was performed prior to deafening and again one to two weeks post treatment. ABR was determined at 2, 4, 8, 16, 24 and 32kHz tone pips (5ms) and clicks (ΙΟΟμβ, alternating polarity), while DPOAE was tested at 6, 8, 1.6, 20, 24 and 28 kHz. ABR thresholds were tested to a maximum level of 90dB SPL while DPOAE was evaluated 60dB SPL, Only animals lacking ABR or DPOAE were used in the gene therapy experiments.

Tissue processing following in vivo electroporation

| ^' 00158j Fixed cochleae were decalcified in 8% EDTA in 0. IM sodium phosphate buffer at 4°C for two weeks (EDTA solution was changed every 2-3 days). To determine transformed cell counts of in vivo electroporation-alone animals, decalcified cochleae were cut with a scalpel along the modiolar axis, removing the topside of the bony capsule of the cochlea. GFP positive cells on each hemiseetion could then be located and counted with an epifluorescence microscope.

[0 159| To analyse neurite maintenance and outgrowth, decalcified cochleae of deafened and treated guinea pigs were cryoprotected in 10% sucrose for 24h and 30% sucrose for 24h. Cochleae were embedded using Tissue-Tek OCT (Sakura Finetek), snap frozen at -8Q°C and cryosectioned at SOprn. Mid-modiolar sections were placed into 32 well plates containing OJM PBS for ttiiiiiunolahe!tng. Cociileae of one guinea pig, also deafened and treated were not cryosectioned. These cochleae were hernisectioned as was done for the electroporation- alone animals and mmunostaining was performed in sittt on whole-moun tissue to provide a three dimensional view of the neurite outgrowth (Fig. 14 a, b).

Iiiiniiniohistoelieniistry

[00160] Tissue was immunolabeled in 12 well plates containing OJ M PBS. 10% normal goat serum (NGS) was included as a blocking agent along with 1% Trito X- 100 for pemieabilization. The tissue was incubated at room temperature for one hour prior to addition of the primary antibody (with 5% NGS and 0.1% Triton X-100). Primary incubation used a 1 :8000 dilution of anti-rieoronal class III β-Tubulm (FUJI) (Covanee) or 1 :500 anti-FLAG (F7425) (Sigma) or 1 :400 ami-aspartoacylase (ASPA) antibody. Secondary antibodies used were Alexa-488 conjugated goat anti-mouse (Al iOOl) (Invitrogen) for class Hi β-Tubiiim, and Alexa-594 conjugated goat anti-rabbit (Al 1072) (Invitrogen) for FLAG and ASPA primaries. Both primary and .secondary staining was done overnight at 4°C in PBS containing 5% goat serum and 0,01 % Triton X-100. Three washes of 10, 20 and 40 minutes were done after incubation with each antibody.

Image acquisition and statistical analysis

[00161] Semi-quantitative analysis of spiral ganglion neurite regeneration and increase in SGN somata cell size was perforated using β-tubulin immunofluorescence data. Images were acquired using a confocal laser scanning microscope (Zeiss LSM 710 NLQ). Three mid-modiolar sections were imaged for each cochlea and three optical slices were captured and exported as separate TIFF files for each section. The optical slices were taken at ΙΟμηι intervals through the centre of each section. Exported 8 bit TIFF files were analysed using image J software (National Institutes of Health), images were taken of the basal turn only for both left (treated) and right (control) cochleae. The mean values obtained for the three optical slices were averaged over the three tissue sections to generate a mean value for each cochlea. To calculate the average soma size, the circumference of each SGN cell within RosenthalFs canal was outlined, and the area recorded. The nucleus of each ceil was also measured. It was determined that discounting cells with a nucleus surface are less than 15pm ² would preclude duplicate neuron counting across multiple optical sections. | ^' 00i.62] To quantify the BDNF gene therapy-mediated regeneration of the SON neurites within the osseous spiral lamina, average pixel intensities of the neuronal label ling were calculated. All images were captured using identical settings. The fibre tracks through the spiral lamina were manually outlined in each of the exported TIFF files. The sum of all pixel intensities within that area was divided by the total designated area to give a sum of average pixel intensities per square micron,

00163J Statistical analysis and graphs were generated using SigmaPiot software (Systat®). Treated and untreated cochleae were compared by a repeated measures one way ANOVA. for both cell soma size and dendrite maintenance. A one way ANOVA was performed on ranked data to compare the efficiencies of tandem and alternating arra configurations for the e vivo data, with post-hoc Holm-Sidak multiple pairwise comparison. Data analysis included assessment for normal distribution and equal variance; where required, data were analyzed using non-parametric ranked ANOVA.

Calculating transfection efficiency

[00164| Transfection efficiency calculations were determined based on nuclear GFP labelled mesenchymal cell counts compared with universal nuclear fluorescence labelling with DAPI within regions of interest for ex vivo imaging of dissected fixed cochleae (Fig. 12 a, b).

Electrically-evoked auditory brainstem response (eABR) measurements

[Q016 ^' 5| eABR was measured under isofiurane anaesthesia. The guinea-pigs were implanted with the 8 node electrode arrays (Cochlear ltd. Part number Z60274) as described for the microarray-based electroporation methodology, except that the implant was cemented in place (Ketac Cem Easymix dental cement. 3 ESPE AG, Germany), securing the array within the cochlea and emerging lead to the mastoid bone. The array lead was routed from the bulla to the scapulae, where the connector was implanted subderma!iy, encapsulated in parafilm. To undertake eABR measurements, the guinea-pigs were anaesthetised with isofiurane and an incision was made to access the microarray connector. This was attached to a stimulator (A-M systems analog stimulus isolator 2200, USA) set to provide stimulation via a bipolar (tandem) configuration. ABE. recording electrodes (subdermal) were placed at the vertex of the skull and lateral temporal region on the implant side; a reference electrode was inserted in the flank. Alternating monophasic pulses of lOOps duration were delivered via the cochlear implant array in the tandem configuration (bipolar), and averaged over 512 trials. A suprathxeshold maximum stimulus was set, with successive IdB reductions driven by the auditory evoked workstation (as described for ABR recordings under Deafness model) in referenced to 1mA at 70dB. The pi waveform latency of the eAB occurred around 400us from the stimulus onset, eABR amplitudes were measured as the i -nl difference (Fig 16a),

Example 2 - Close-field Microarray-based Electroporation of confluent HEK293 cells

[Θ0166) HE 293 ceils grown to confluence on covers-lips coated with poly-D-lysine were overlayed with a solution comprising a brain derived neurotrophic factor (BDNF) gene therapy cassette (CMVp-3xflag-BDNF-IRES-hrGFP gene cassette) as described above, and subjected to electroporation. Electroporatiott was via a microarray comprising an 8 electrode linear microarray consisting of platinum ring electrodes 400 μτη diameter x 350 μτη depth (Cochlear Ltd, Australia, part no. Z60276). The microarray was placed on top of the confluent cells.

[001671 Parameters varied were electrode configuration and electronic stimulation treatments.

[00168) Electrode Configurations were as shown in Fig.3:

Tandem: four cathodes(-) then four anodes (+) - tip - total distance = 5.4 mm;

Alternating (anode-cathode-anode-cathode- anode-cathode-anode-eathode) - total distance = 5.4 mm;

Electrodes 1 and 8 - electrode position 1 = anode (+); electrode position 8 = cathode (-) (separation = 6 mm). The electrodes in the centre were not connected; and

Electrodes 1 and 2 - electrode position 1. = anode (+); electrode position 2 = cathode (-) (separation = 350 μτη). The other electrodes progressing to the tip were not connected.

[00169} Electrostimulation treatments (voltage and pulses) were varied as follows (all pulses were 50ms with 950 ms interval, using a COY 21 square wave eiectroporator ; NEPA GENE):

40V/ 10 pulses (40V/10P);

20 V/ 10 pulses (20V/10P) and

1.0 W 5 pulses (10V5P)

[00170] 8 experiments were carried out per configuration/treatment combination. [001711 Transfected cells expressing GFP were counted at 48 hours post-eleetroporatton, using an epifkiorescenee microscope., after fixing in 4% paraformaldehyde and washing in phosphate buffered saline (PBS).

[00172) Data analysis ranked two way ANOVA with Hoim-Sidak pair- ise comparison. Results:

[00173) The data, summarised in Fig. 4, showed that eiectroporation of a planar sheet of HEK293 cells continued the findings of the in vifro and in viva studies in showing that the 'tandem' electrode configuration provided higher transfection efficiency than the 'alternating' electrode configuration (PO.001 ).

[00174] The tandem electrode configuration also provided significantly greater numbers of transfected cells than any of the other three configurations (P<0.001 ).

[00175] Fig. 4 shows that all configuration/treatment combinations provided significantly greater numbers (P<0.Q0 L) of transfected cells than the control (no eiectroporation - DNA present), except for the 20V/5pulse treatment with the 1 and 8. electrode configuration (P— ,076), and the alternating, 20V/5P treatment (P=G.00 ). This means that the minimum configuration of 2 electrodes only 350pm apart (config .1 and 2) was significantly effective, and a tandem configuration (with the same minima! gap between anode and cathode) provided the best results. The configuration of el ectrodes 1 and 8 was nonetheless effective , especially at the higher voltage/pulse regimes. The difference in effectiveness between the alternating and tande electrode configurations appears to correlate with the electric fields modelled for these configurations (see Fig. 2A), which show the tandem electrode configuration as providing the most strongly focussed electric field.

[00176] The eiectroporation effect extended from < 400um from the electrodes to a distance of - 2.5mm.

I O0177| Figures 5 and 6 illustrate transfection results for this in vitro model of CFE utilising confluent sheets of HEK293 ceils. Figures SA to 5F show the 8-eJectrode linear array as placed on top of the cells, as a 2D model to test for the difference in transfection efficiency between the alternating and tandem electrode configurations. Cells were treated with 10 40V pulses at 50ms per pulse. The arrowheads indicate regions of transfected ceils displaying nuclear localization of GFP fluorescence 48 hours post-transfection. The greater efficiency of the 'tandem' microarray configuration (Tigs 5A, 5C and 5E) over the 'alternating' microarray configuration (Figs 5B, 5D and 5F) is clearly evident. Note the close-field effect where cell transfection extends to a boundary approximately 2.5 mm from the array (Figs. 5 and 6). This provides spatial regulation of cell transfection.

Example 3 - Ex vivo cochlear gene delivery

[Θ0178Ι An ex vivo model was developed to establish the potential of icroarray-based close-field electroporation gene delivery. The CMVp-BDNF3x LAG-IRES-GFPnls gene cassette (described in Example 1) was microinjected into the scala tympani perilymphatic fluid compartment of normal intact guinea-pig cochleae, via the round window, and then an eight-node cochlear implant microarray was inserted (Fig, 1 c, d). The microarray was configured as either al.ternati.ng anodes and cathodes ('alternating' configuration), or as a 'tandem ^* array where four adjacent electrodes (350 μηι diameter platinum rings with 300 urn separation) were wired in parallel as the anode (distal end of the array), and the other electrodes provided a common cathode ('tandem ⁵ configuration) (Fig. I d). Transduction of cells with the gene cassette was achieved across the tested voltage range (4 V, up to 100 V; 5 - 20 pulses, 50ms duration) in 76 experiments (Table 1). The lateral walls of the cochleae were removed and the tissue placed in organotypic culture ten minutes after electroporation. From 18 hours in culture, GFP reporter fluorescence was prominent in mesenchymal cells lining scala tympani and scala vestibuli including the perilymphatic surface of Reissner's membrane (Fig. 7a,b, Fig. 8, and Fig. 9 b, c, d), and was maintained for the duration of organotypic cultures (up to 4 days). The GFP positive cells were confined to the basal turn region, within -lrmri of the array, consistent with spatially constrained electroporation of the cells within the cochlear perilymphatic chambers. Middle and apical regions of the cochlea were devoid of GFP positive cells (Fig. 7c). Control experiments where DNA was delivered without electroporation yielded minima! GFP positive cell counts (mean = 3 ± 0; n = 2). Cryosectioning of electroporated cochleae revealed that the transduction was effectively constrained to mesenchymal, cells (Fig. 9b). Hair cells and supporting cells of the organ of Corti, or other cell types facing scala media, were not transduced. Expression of BDNF by transduced cells was confirmed by anti-FLAG immunolabeling in both whole-mount and sectioned tissue (Fig. 7d, Fig. 9d), CFE was effective from 10V in both microarray configurations (combined data 26.4 ± 8.3, n = 5; P=0.033; one sample t-test; Fig. 7e).

[001791 The tandem microarray configuration demonstrated greater gene delivery efficiency than the alternating configuration, with an asymmetric optimum in the lower range of applied voltages (~20V) and a minimum effective charge delivery of 0,01 coulombs (Fig 7 e,f). Cell counts of up to 310 GFP positive cells were achieved with five 20V pulses, with no increase with additional pulses. In contrast, the alternating Table I .: Summary of nuclear GFP label led mesenchymal cell counts arising from cochlear close-field electroporation gene delivery.

n/d - not determined, n a - not applicable; GFP+ is the number of transformed mesenchymal cells showing nuclear localized green fluorescence protein signal,

*based on passive resistance measurement; total charge delivered to array.

**Note: Control experiments where DNA was delivered without electroporation yielded minimal GFP positive cell counts (mean ± s.e.m. ^~ 2.8 ± 0.5; n = 5). Close-field

electroporation was effective from 4 V (combined mean = 9.5 ± 1.8, n=8; P = 0.017; t-test). configuration provided a linear increase in transformation efficiency with increasing voltage to the highest test level and charge delivery (100V, 0.416 coulombs Fig. 7 e,.f). Comparing transfectants between 'tandem' and " alternating' array configurations for ail data between 10V and 40V showed a significantly greater efficiency with the 'tandem' array (P ^~ 0.039, ranked one way AN OVA).

Example 4 - In vivo cochlear gene delivery

[00180] in 16 experiments, a cochlear implant microarray was surgically inserted into anaesthetised guinea-pigs for CFE gene delivery (Fig. 10). In vivo CFE yielded comparable transformed cell numbers to the ex vivo experiments (compare Figs. 7, 8 and 9b with Figs. 9a and 11 ; and Table 1 with Table 2).

[001811 The optimum ex vivo transfection parameters for the two array eon figurations were mapped to in vivo experiments. 'Tandem' CFE produced more consistent levels of transfection in vivo than did 'alternating' CFE (tandem: 20V - 5 pulses, range 45 - 373, mean = 169.1 ± 47.9, n = 7; alternating: 40V - 20 pulses, range 1 - 99, mean = 47.4 ± 21.5, n = 5). in addition, transduction via alternating CFE at the 20V - 5 pulse parameter was 8- fold less tha tandem CFE (15.5 ± 9.0, n = 4) (Fig 1 lc; Ρ=0.043, t test). All cochleae were fixed 3 - 4 days after electroporation. Maximum transfection efficiency of the tandem array configuration was determined in three experiments (20V - 5 pulses) at 4 days post CFE. These experiments yielded GFP cell counts ranging from 179- 373 cells. Regions of interest that included at least 100 ceils were analysed for GFP and DAPI fluorescence nuclear labelling (Fig, 12). The average number of double-labelled mesenchymal cells was 42.5% ± 3.3%. Two control experiments utilized electroporation with a GFP-nuii vector; no GFP fluorescence- cells were detected. Two controls where the left, cochlea was perfused with the GFP expressing BDNF plasmid followed by insertion of the electrode array, without electroporation resulted in 6 and 7 GFP positive cells, respectively, four days post- electroporation (not shown).

Example 5— In vivo BDNF gene therapy promotes spiral ganglion neu ite regeneration |0Q182| A kanatnycin - furosemide ototoxic treatment was used to selectively destroy sensory hair cells and produce a deafened guinea-pig model, where, within two weeks, the peripheral neurites of the SGN degenerate withi the osseous spiral lamina and the SGN somata atrophy (see Example 1). Both alternating configuration CFE and tandem configuratio CFE BDNF gene delivery to the left cochlea produced pronounced regeneration of tire peripheral spiral ganglion neurites, and increased the size of somata Table 2: Summary of GFP counts of transfeeted mesenchymal cells with close-field eieetro oration in vivo.

*based on passive resistance measurement; total charge delivered to array; GF.P+ is the number of tr ansformed mesenchymal cells showing nuclear localized green fluorescence protein signal. compared with the untreated (right) cochlea of deafened guinea pigs (analysed from 7 - 21 days post gene delivery) (Figs. 13, 14, 15). The extent of regeneration of the peripheral auditory nerve was determined by analysing the intensity of β-tubulm immunofluorescence within the osseous spiral lamina region (basal turn) ( -0.017, comparing matched treated and untreated cochleae of each of four animals, paired t-test; Fig 13d, Table 3). Similarly, the average SG somata size was approximately 40% larger in the BDNF gene therapy- treated (left), versus untreated (right) cochleae of these animals (basal turn, / -0.012, paired t-test; Fig. 13e, Table 3). The deafened guinea pig organ, of Cord typically exhibited a flattened epithelium due to the loss of hair cells and supporting cells (Fig. 13b, Fig, 15a). Neurite processes typically branched beyond the habenula perforata and descended through the basilar membrane into the scala tympam region (Fig. 13a, Fig. 15). Ectopic branching also extended to the inner sulcus, and outer sulcus - spiral ligament region, within scala media (Fig. 14, Fig. 15). The neurite regeneration extended beyond the basal turn mesenchymal cell transfection zone, to the mid-turn region (Fig. 13a), likely reflecting diffusion of the secreted recombinant BDN F. Robust immunolabeling for the Schwann cell marker aspartoacylase (Mersmaiut, N. etal (201 1), "Aspartoacylase-lacZ knockin mice; an engineered model of Canavan disease", PLoS One 6, e20336) indicated remyelinatioii of regenerated neurites (Fig. 1 c). Table 3; Animals histologicall examined for neurite regeneration following close-field electroporatiott gene delivery - compar son between treated and untreated cochleae from the same animals.

Animal ID Days between Days between Average SGN Average pixel deafening and therapy and cell soma size intensity per therapy tissue collection am ² dendrites

Treated Untreated Treated Untreated |

GP287 16 1.7 215.9 149.3 60.0 21 .4 J

GP284 1.7 1 1 257.3 200.4 33.0 14.3 1

GP283 1 ¹⁴ 1 297.7 192.6 73.2 19, 1 1

GP269 21 21 216.9 169.9 40.5 10.0 1

*For guinea pigs 287, 284. and 283, alternating configuration of the array was used. For guinea pig 269 a tandem configuration was used.

Example 6 - Enhanced cochlear implant performance

[00183] Thresholds for electrically-evoked auditory brainstem response (eABR) via the cochlear implants were determined four weeks post-deafening, which was two weeks after cochlear implantation and tandem configuration CFE gene delivery (20V - 5 pulses) using either the BDNF-GFP gene cassette (n=5), or the control (GFP) vector (n=5). The improvement in auditory brainstem neural recruitment due to the BDNF gene therapy was such that the mean eABR threshold for tire BDNF gene therapy group was less than half that of the control group (BDNF group 145.6 μΑ ± 19.3; control group 3 19 μΑ ± 26.0; P<0.001, ANOVA, Holm-Sidak all pairwise comparison) (Fig. 16; Table 4), with no overlap in data (Fig. 16c).

[00184] These results show a considerably greater improvement in cochlear implant performance than that reported for direct BDNF drug delivery in an earlier study (Landry, T. G.et al (201 !), "Spiral ganglion neuron survival and function in the deafened cochlea following chronic neurotrophic treatment", Hear Res 282:303-313). Complementing this, the dynamic range (growth function) of the eABR was significantly increased in th BDNF gene therapy treatment group (Fig. 16 b, d), with a considerable extension of the range of stimulus eliciting growth of the e ABR, Thus BDNF gene therapy restored the cochlear Table 4: eABR. Results following close-field eleetroporation gene delivery of a BDN ^' F therapeutic cassette versus GFP control cassette.

nerve recruitment towards pre-treatment levels (Fig 17). By way of comparison, acute cochlear implant stimulation in normal-hearing guinea-pigs (where bot the auditor}' ne 'e and the sensory hair cells are intact; Fig. I7a,c), provided a mean eABR threshold of 151.2 Α ± 19.4 (n = 5). This was not significantly different from the BDN ^' F gene therapy group (P^O.771 ; one way ranked ANOVA, Ho n-Sidak all pairwise comparison) (compare with Fig. 16c), indicating recover of threshold, e en discounting possible augmentation of this baseline in this control group due to inner hair cell stimulation. The baseline for the CFE-mediated BDNF gene therapy was two weeks post deafening. Reference eABR. recordings at the time of implantation had a mean threshold of 223.7μΑ ± 7.4 (Fig. 16 b,e; n = 10). This was significantly higher than the BDNF gene therapy group at 2 weeks treatment (P <0.001; see Fig. 16c), but significantly less than the GFP vector control group at 2 weeks treatment (f -0.001 ; Fig. 16c), indicating the further deterioration in the spiral ganglion over time in the absence of gene therapy. The functional, recovery of eABR input- output gain with BDNF gene therapy was re-confirmed at four weeks post-treatment (6 weeks after deafening) in cases where the interface had not failed n = 2 BDNF-GFP vector; n = 4 GFP only control vector) (Fig. 18). Overall, these results demonstrate a remarkable flmctional enhancement of the cochlear imp! ant -neural interface arising from restoration of the peripheral spiral ganglion neurites following CFE-mediated BDNF gene therapy. Example 7 - Analysis of Pulse Width

[00185] Here pulse width was assessed for transduction of HEK293 cell monolayers using the 8 electrode microarray in Tandem configuration for close filed eleetroporatoin.

[001861 The cells were transduced as described in Example 2. in this example of close field electiOporation, voltage and pulse number were kept constant at 40V and 5 pulses respectively, and the square-wave pulse width was varied between 100μ$ and 100ms, with a GFP reporter cDNA construct at 2μ£ μΤ in TRIS-buffered saline. After 48 hours in culture, the GFP positive cells were fixed and then counted as GFP positive labelling. These data were best fit by the relationship: f = 249* (1 -exp( -0.025*x)). Data shown are mean +/- s-.e.m.. n = 3 - experiments for each pulse duration; 40 Volts, 5 pulses of varying duration (plus a 0 V, 0 pulse control, n = 5). These dat (Fig. 19) indicate that 90% of the maximum possible electroporation efficiency is achieved with pulse widths of 100ms; 50ms pulses enable 78% of the maximum efficiency; 40 ms represents the decay constant for this exponential curve, i.e. where 67% of possi ble transduction occurs. Thus at 5 decay constants (200ms), these data predict 99% maximum transduction.

Discussion

[00187| This study indicates that close-field electroporation of naked DNA utilising a cochlear implant microarray enables high efficiency gene delivery. Since the electric field strengtli decays quadraticaily with the distance from electrodes, the mieroarray-based CFE allows site-specific transformation of the mesenchymal cells lining the perilymphatic compartments of the cochlea. This somatic cell gene therapy produced the neurotrophm support critical to the viability of the primary auditory neurons, a. signal lost with the death of the cochlear hair cells. In response to BDNF expression by the mesenchymal cells, the regeneration of the peripheral auditory neurites extended back along their original track within the osseous spiral lamina. This porous structure is contiguous with the perilymph and the re-growth in mid-cochlear levels likely reflects apical diffusion of BDNF from the basal transduction site. BDNF expression drove regeneration of SGN peripheral neurites to close proximit with the electrode array. This regeneration, accompanied by remyelination and increased, somata size, make the spiral ganglion neuron highly amenable to more precise electrical stimulation, with reduced stimulus intensity. The study also demonstrates that the configuration of the electrodes within the microarra is a major determining factor for CFE transformation efficiency. Gene delivery was most efficient using a 'tandem' array configuration, compared with the 'alternating' electrode configuration. This points to the nature of the shape of the current paths around the electrodes as playing a role in the transient dielectric breakdown of the cell plasma membrane that enables movement of negatively charged DNA into the cells. There was minimal transduction of other cell types (within the cochlear partition) and this is probably due to the limited diffusion, of DNA from the perilymphatic compartment in the few minutes required for DNA loading and CFE. The eABR input-output function of the BDNF gene therapy-treated cochleae was restored towards control levels (2 weeks post deafening, Fig. 17 c,d), indicating that recruitment of smaller sub-populations of spiral ganglion neurons may be achievable with increased electrode density, due to lowered stimulus thresholds. This has significant transiational ramifications for the performance of future generation cochlear implants utilising such complementary gene therapy. Currently pitch perception is largely lacking in cochlear implant recipients, making tonal colour and music appreciation a major potential gain if the microarray - spiral ganglion nexus can be improved.

[00188| This microarray- based CFE should have broad transiational significance as it overcomes many constraints of other gene therapy platforms, including the use of naked DNA, delivered by highly localised electrical fields without significant current spread, reducing extraneous electrical stimulation and local tissue damage. In the case of the cochlear implant array, the ability of CFE utilizing a few pulses of -lOV to produce significant numbers of transformed cel ls is wel l within the operational parameters of current generation cochlear implant devices and other neural interfaces.

[00189] BDNF neurotrophin gene therapy demonstrated here has broad applicability to neural repair, but the application i not limited by particular gene constructs. For example, it is contemplated that applications of the present methodology will include treating brain disorders, such as Parkinson's disease, where CFE may provide a safe and efficient alternative to proposed viral vector-based transduction of neurons and glia in the striatum for sustained production of dopamine. Safety concerns are considerably mitigated in comparison with viral vector-based gene therapy approaches, where uncontrolled spread of the virus can lead to undesirable off-target effects, and the likelihood of immunological reactions is a major barrier to implementation. Along with the advantage of using circularized naked DNA which does not integrate into the genome, thereby eliminating the possibility for random insertional. mutagenesis, CFE gene therapy also removes the DNA packaging constraints associated with viral-vectors. The therapeutic gene cassette can either be directly delivered to the target site, or integrated into the microarray interface, while the microarray itself could be used acutely, for CFE-mediated gene therapy alone, or chronically, implanted to establish an enhanced neural interface, as shown in the deafened cochlea model exemplified here.

Example 8 - Further studies investigating electroporation parameters

[00190] Further studies were carried out to further characterise parameters important for optimal electroporation, including electrode array configuration, voltage applied, number of electroporation pulses, pulse duration, pulse gap, and investigation into CFE-mediated cell death.

A) aterials and Methods

HEK-293 Cell Line

[001 1 j The cells were maintained, in Dulbecco's Modified Eagles Medium (DMEM) supplemented with 10% Heat-Inacti vated Fetal Bovine Serum (HJ-FBS, Life Technologies), 1 % Peniciilin/Stieptomycin mix (Life Technologies), 1% Non- Essential Amino- Acids (NEAA. Life Technologies). Cells were split and plated on coverslips the day prior to electroporation. Cells were electroporated at 80% confluence.

DMA Plasmid

[00192] The construct used in these experiments contained a nuclear localized green fluorescent protein reporter gene (GFPnls), driven by a chicken-B-actin/cytomegalo virus hybrid promoter (CAG). Plasmid DNA was purified from E. coli using a column chromatography purification kit (Qiagen) and resuspended at 2pg/pl and in 50mM Tris- buffered saline (pH 7.4) before transformation.

Close-field Electroporation

[00193] Electroporation was performed on the HEK293 cell monolayers with an In Vivo Square Wave Electroporator (Sonidel Ltd, CUYEDIT), connected to a linear eight-node cochlear implant array consisting of platinum ring electrodes (400 μηι diameter x 350 μτ length) with 350 pro separation (Cochlear Ltd, Australia, part no. Z60276). The coverslip was removed from the cell culture well and placed on a flat surface. The electrode array was lowered onto the coverslip. The DNA was applied to each coverslip (20μ1 at 2pg/pl) electroporation was initiated using the desired electrode configurations and electroporation parameters. Study of CFE-mediated Cell Death

[00194) Using the 'Tandem' array configuration, the pulse was set at 40V, L 5 or 40 pulses, 40ms pulse duration, 1 pulse/sec. Tris -buffered saline was applied to the covers lip instead of the DNA. Immediately after electroporation, coverslips were placed in a humidity chamber for 2 mins. Coverslips were then placed in fresh, warmed media and returned to the incubator for 30 minutes. Cell death was identified by propidium iodide fluorescence, where propidium iodide was added to the medi at a concentration of 1.1000 and was incubated for 5 minutes. Coverslips processed and imaged using the confocal LSM with 633 nm excitation using a HeNe laser.

B) Study of variation in electrode array configuration on CFE

[00195) The electrodes within the array were configured as anodes and cathodes with the following arrangement (illustrated in Fig. 20): 1+2 (a single anode and a single cathode with 350 pm separation), 1+5 (a single anode and a single cathode with 2.45 mm separation), 1+8 (a single anode and a single cathode with 4.55 mm separation), tandem (four juxtaposed cathodes, then four anodes, all elements with 350 um separation; total length 5.4 mm) and alternating (alternating cathode and anode with 350 pm separation; total length 5.4 mm).

[00196] This initial study of the effect of varying electrode configuration on transformation efficiency and spatial distribution used 40V, 10 pulses, 50ms duration, 1 pulse/sec.

[00197) Significant numbers of cells were transformed by CFE, evident as green fluorescence in the cell nuclei (Figs 21 A to 2 I F). The numbers of cells and spread of cells provided a readout of the effective electric field (Fig. 21).

[00198] The results demonstrate that there is a significant effect of vary ng electrode spacing and electrode association. Ranked One-Way AN.OVA showed that the configiiration of the electrodes with in the array had a significant effect on the number of transformed, cells (P<0.Q0i; n - 4 per configuration). All CFE configurations produced significant cell transformation compared with the control group (Fig. 22: Table 5; ranked one way ANOVA with Ho ^' lm-Sidak pairwise comparisons (P<0.001 for all except M+8' (P=0.016), n = 4 per group)).

1 _. 00199] The control group (no CFE) exhibited 0.8 + 0.8 OFF positive cells per coverslip. The different CFE configurations resulted in variation in numbers of transformed cells between 7.0 ± 3.1 (Ί +8') and 219.0 ± 37.7 ('tandem'). The 1+2 CFE resulted in a circular field of cells with the electrodes at the centre (Fig. 21 A) of - 1mm diameter, whereas the alternating CFE produced a linear bias to the field of transformants extending the length of the array (- 5mm) Fig. 2 I E). 1 +5 and .1+8 CFE configurations yielded smaller average transformant numbers, which had a broad, low density distribution (Figs. 21C, D). The transforation efficiency with the tandem configuration was significantly higher than any of the other configurations (see Table 5). These data suggests that a key feature of CFE is sub mm separation between the anode and cathode poles within the array; highlighting the difference between transformation using local electric field focusing and conventional 'open- field ^* electroporation. where the target field is located between separated electrodes.

Table 5; Statistical analysis for CFE electrode configuration by one-way ANOVA with Holm-Sidak post-hoc comparisons (n= 4/group)

1+2 vs. Ait 1 i.ooo 0.050 j No

B) Electrical Pulse Parameters

[0021)0] Utilising the most efficient CFE electrode configuration (tandem), the effect of electrical pulse parameters was systematically resol ved. i) Voltage amplitude

[00201] Using the 'Tandem' array configuration, the pulse parameters were set at 10, 20 or 40V, 10 pulses, 40ms pulse duration, 1 pulse/sec.

[00202] The numbers of transformed cells increased significantly with increasing pulse amplitude (40ms duration, 10 pulses, l/s; Fig. 23; Table 6). The 40 V amplitude produced -8 times greater transformation than the 20 V amplitude (170 + 19.7 versus 22,7 ± 5.9; n - 6 pe group). No transformed ceils were detected in the control experiments (n = 6). Table 6: Statistical analysis for voltage amplitude by one-way ANOVA with Tukey's Test post-hoe comparisons (n= 6/group)

ii) Pulse number

[00203] Using the 'Tandem' array configuration, the pulse was set at 10 or 20V, pulse number ranging from 1 - 40 (I, 3, 5, 10, 20 and 40), 40ms pulse duration, 1 pulse/sec,

[00204] Forty volts produced significantly greater cell transformation than twenty volts across the range of pulse numbers (2 way ranked ANOVA, P<0.001). At 40V, all pulse numbers (1, 3, 5, 10, 20, 40) resulted in. significantly greater transformation than the control (no CFE); ranked ANOVA, multiple Comparisons versus Control Group (Holm-Sidak method; P<0.001 above 1 pulse; P= 0.031 1 pulse). Cell transformation had a bell-shaped distribution with regard to pulse number with tandem configuration CFE (40V, 40 ms; n = 6 per gro p except for 5 pulses (n=9)) (Fig. 24; Table 7), with 5, 10 and 20 pulses providing the maxima (range 147 - 170 cells) with no significant difference between these treatments (ANOVA, Holm-Sidak comparisons). At 20V, 3, 5, 10 and 20 pulse numbers resulted in significantly greater transformation than the control (no CFE); ranked ANOVA, multiple Comparisons versus Control Group (Holm-Sidak method; P<0.003). Ceil transformation had a bell-shaped distribution with regard to pulse number with tandem configuration CFE (20V, 40 ms; n = 6 per group) (Fig. 24; Table 7), 10 pulses providing the maxima (22 GFP +ve cells). There was a decline in transformed cell numbers at 40 pulses for both 20 and 40V. This was explained by the noticeable increase in cell death proximal to the electrode array with prolongation of the pulse train detected using propidium iodide fluorescence (Fig. Table 7. Statistical Analysis for pulse number, using A) 20V (n=6/grotip) and B) 40 V (it=6/group (except 5 n= /group)), 40 ras duration pulses, by one-way ANOVA with Holm-Sidak post-hoc comparisons

A) 20V pulses

Comparison j Diff of t Unadjusted Critical Significance

1 Means P Level

20V: 10 pulses vs. 1 pulse j 18,250 3.642 <0.001 0.003 Yes

20V: 10 pulses vs. 40 pulses j 17,000 3.393 0.001 0.004 Yes

20V: 5 pulses vs. 1 pulse j 13,583 2.7.1 .1 0.009 0.004 No

20V: 5 pulses vs. 40 pulses I 12,333 2.46! 0.017 0.004 No

20V: 10 pulses vs. 3 pulses j 9.583 1.913 0.060 0.005 No

20V: 10 pulses vs. 20 pulses j 9.417 1.879 0.065 0.005 No

20V: 20 pulses vs. 1 pulse j 8.833 1.763 0.083 0.006 No

20V: 3 pulses vs. 1 pulse j 8.667 1.730 0.089 0.006 No

20V: 20 pulses vs. 40 pulses j 7.583 1.513 0.135 0.007 No

20V: 3 pulses vs. 40 pulses j 7.417 1.480 0.144 0.00 :NO

20V: 5 pulses vs. 3 pulses j 4.917 0.981 0.330 0.010 :NO

20V: 5 pulses vs. 20 pulses j 4.750 0.948 0.347 0.013 No

20V: 10 pulses vs. 5 pulses j 4.667 0.931 0.355 0.017 No

20V: 40 pulses vs. 1 pulse j 1.250 0.249 0.804 0.025 No

20V: 20 pulses vs. 3 pulses j 0.167 0.0333 0.974 0.050 No

B) 40V pulses

Comparison Unadjusted P itical Level Significance

40V: 10 pulses vs. 1 pulse <0.001 0.003 Yes

40V: 5 pitlses vs. 1 pulse <0.001 0.004 Yes

40V: 20 pulses vs. 1 pulse <0.001 0.004 Yes

40 : 10 pulses vs. 40 pulses <0.001 0.004 Yes

40V: 10 pulses vs. 3 pulses 0.0 1 0.005 Yes

40V: 20 pulses vs. 40 pulses <0.001 0.005 Yes

40V: 5 pulses vs. 40 pulses 0.001 0.006 Yes

40V: 20 pulses vs. 3 pulses 0.003 0.00 Yes

40V: 5 pulses vs. 3 pitlses 0.004 0.007 Yes

40V: 3 pulses vs. 1 pulse 0.079 0.009 No

40V: 40 pulses vs. 1 pulse 0.221 0.010 No

40V: 10 pulses vs. 5 pulses 0,234 0.013 No

40V: 1.0 pulses vs. 20 pulses 0.471 0.017 No

40V: 3 pulses vs. 40 pulses 0,575 0.025 No

40V: 20 pulses vs. 5 pulses 0.682 0.050 No lii) Pulse duration

[00205| Using the 'Tandem' array configuration, the pulse was set at 40V, 1 or 5 pulses, and a range of pulse durations (0.1 -400ms) with appropriate gap durations (999.9-600ms) (which ⁼ I pulse sec).

[002061 All pulse duration experiments other than 0.1 ras for 1 pulse produced significant CFE —mediated cell transformation (P 0,004; ranked ANOVA; Holrn-Sidak multiple comparisons for I pulse; Mann-Whitney Rank Sum Test for 5 pulses, G.Iras; P=0.026). Cell transformation was maximum at 100 ms pulse duration. Five pulses produced significantly greater cell width (2 way ranked ANOVA, 10 ras - 400 ms indicating a significant interaction between pulse number and pulse duration; P<0.Q01); Fig 26, Table 8,). While with a single pulse, there was no difference between 40 ms (38.3 ± 5,1 cells). 100 ms (51.7 ± 7.8 cells; - 6) and 400 ms (49.2 ± 6.9 cells; n - 6) (P>0.05; Holm-Sidak multiple comparisons), the 5 pulse treatment showed that 40 ms (147.2 ± 12.4 ceils) and 100 ras (211.7 ± 1.6.6 cells) pulse durations produced significantly greater cell transformation (P=0.003; P<0.001 ), The 400 ms pulse duration with 5 pulses resulted in a 5,5 fold decline to 38.3 ± 10.2 cells; n = 6 per group) (P<Q.00 i ; ranked 2 way ANOVA, Holm-Sidak multiple comparisons).

Table 4. Statistical Analysis for pulse duration with 40 V, 40 ms duration and with 1 pulse (n=6/group) or 5 pulses (n=6/group (except 40 P n=9/group)), ranked two-way ANOVA wit Holm-Sidak multiple comparisons.

Comparison Diff of t Unadjusted Critical Significance

Means P Level

I P: 100 ms vs. 10 ins 13.583 3, 145 0.003 0.009 Yes

1 P: 400 ms vs. 10 ms 12.750 2.952 0.005 0.010 Yes

IP: 100 ms vs. 40 ras 7.417 1.71.7 0.093 0.013 No

IP: 400 ms vs. 40 ms 6.583 1.524 0.135 0.017 No

IP: 40 ms vs. 10 ms 6.1.67 J .428 0.1.61 0.025 No

IP 100 ms vs. 400 ms 0,833 0, 193 0,848 0.050 No

5P: 100 ms vs. 400 ms 32.417 7.505 0.00! 0.009 Yes

5P: 40 ms vs. 400 ras 26.722 6.777 <0.001 0.010 Yes

5 : 100 ms vs. 10 ms 1 7.91 7 4.148 <0.001 0.013 Yes

5P: 10 ms vs. 400 ms 14.500 3.357 0.002 0.017 Yes

5P: 40 ms vs. 10 ms 12,222 3.100 0,003 0.025 Yes

5P: 100 ms vs. 40 ms 5.694 1 ,444 0.1.56 0.050 No Comparisons for factor: pulse no within 10 ms

Comparison Diff of Means t U n ad j listed P Critical Level Significance

5p vs. Ip 21.000 4.862 <0.001 0.050 Yes

Comparisons for factor; pulse no within 40 ms

Comparison Diff of Means t Unadjusted P Critical Level Significance 5p vs. Ip 27.056 6.862 <0.00l 0.050 Yes

Comparisons for factor: pulse no within i 00 ins

Comparison Diff of Means t Unadjusted P Critical Level Significance

5p vs. 1 p 25.333 5.865 <0.00i 0.050 Yes

Comparisons for factor; pulse no within 400 ms

Comparison Diff of Means t Unadjusted P Critical Level Significance lp vs. 5p 6.250 1.447 0.155 0.050 No iv) Gap duration

Using the 'Tandem' array configuration, the pulse was set at 40V, 2 or 5 pulses, 40ms pulse duration, and a range of gap durations (50ms, 100ms, 250ms, 500ms and 1.000ms),

[00207] Immediately after electroporation, coverslips were placed in a humidity chamber for 2 mills. Coverslips were then placed in fresh, wanned media and returned to the incubator. At 48 hours post-electroporation, cells were fixed with 4% paraformaldehyde for 30mms. The cells were then gently washed with phosphate-buffered saline (PBS) for ISmins. Coverslips were then mounted on microscope slides with Prolong Gold with DAPT mountin medium (Life Technologies). The slides were left to cure at room temperature for 24 hours in a darkened box. Slides were then refrigerated and imaged as soo as possible. Ceils were imaged using a confocal laser scanning microscope (Zeiss LSM 710) with a 2.5 x objective. Transfected cells expressing GFP were manually counted from the captured images.

[00208| As shown in Figure 27, there was no effect of gap duration on the level of cell transformation (2 pulses P=0.255, 5 pulses P=0.437; two-way ranked AN OVA; Holm-Sidak multiple comparisons). N=3/group (2P) -5/group.

Conclusion

[00209] These studies demonstrate the efficacy of array-based electroporation for therapeutic transgene delivery to the cochlea, and show that a cochlear implant array provided site-specific control of the location and level of transgene expression. The efficacy of the gene therapy was demonstrated by reversal of somata atrophy and regeneration of lost peripheral neurttes of the spiral ganglio neurons in the treated cochleae of deafened guinea- pigs.

[00210 it will be appreciated that, although specific embodiments of the invention have been described herein for the purpose of illustration, various modifications may be made wi thout deviating from the spirit and scope of the in vention as defined in the following claims.