Title:
METHOD AND APPARATUS FOR INCUBATING ANIMAL CELLS
Document Type and Number:
WIPO Patent Application WO/1988/000965
Kind Code:
A1
Abstract:
A method and an apparatus for incubating animal cells in an incubation tank of a mechanical stirring system, wherein a culture solution is stirred while restricting the disturbance of the liquid level and formation of vortex, and an oxygen-containing gas is continuously ejected from a blow nozzle towards the liquid level to form recesses having an inversed conical shape on the liquid level. Accoding to the present invention, the gas-liquid interface area can be increased by the recesses described above and the ejected gas is forced to form the flow from the bottoms of the recesses to the liquid level along the inner liquid surface of the recesses having an inversed conical shape, thereby improving the renewal ratio of the gas-liquid interface. Accordingly, migration of the gas materials can be promoted between the gaseous phase at the upper part of the incubation tank and the liquid phase of the culture solution while preventing bubbling of the culture solution.
Inventors:
ISHIBASHI TADASHI (JP)
KAWAGUCHI HIDEO (JP)
YOSHINO SAEKO (JP)
ODAWARA YOJI (JP)
KAWAGUCHI HIDEO (JP)
YOSHINO SAEKO (JP)
ODAWARA YOJI (JP)
Application Number:
PCT/JP1987/000573
Publication Date:
February 11, 1988
Filing Date:
July 31, 1987
Export Citation:
Assignee:
HITACHI LTD (JP)
International Classes:
C12N5/07; C12M1/06; C12M3/00; C12M3/02; C12N5/02; C12N5/071; (IPC1-7): C12M3/00; C12N5/00
Foreign References:
| JP4419179Y | ||||
| JPS57164600U | 1982-10-16 |
Other References:
Canadian Journal of Chemical Engineering, Vol. 64, No. 4, (1986), S. R. ADAMSON, B. SCHMIDEL (Industrial Mammalian Cell Culture) p. 531-539
See also references of EP 0316449A4
See also references of EP 0316449A4
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