Title:
METHOD AND APPARATUS FOR PREDICTING PROTEIN FUNCTION SITE, METHOD FOR IMPROVING PROTEIN FUNCTION, AND FUNCTION-IMPROVED PROTEIN
Document Type and Number:
WIPO Patent Application WO/1998/033900
Kind Code:
A1
Abstract:
A method for predicting the function site of an arbitrary protein from among total proteins of a biological species with known genomic data or cDNA analytical data, which comprises calculating the frequency of occurrence of oligopeptides constituting the function site of the above protein from the amino acid sequence of the protein, calculating the value of amino acid residues contributing to the occurrence frequency as a value representing the function, and predicting the protein function site by using this representative value as an indication; a function site predicting apparatus for automatically performing the above procedure; a method for constructing a function-improved protein by artificially mutating amino acid residues constituting the function site specified by the above method; and a novel thermostable DNA synthetase obtained by this method.
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Inventors:
DOI HIROFUMI (JP)
HIRAKI HIDEAKI (JP)
KANAI AKIO (JP)
HIRAKI HIDEAKI (JP)
KANAI AKIO (JP)
Application Number:
PCT/JP1998/000430
Publication Date:
August 06, 1998
Filing Date:
February 02, 1998
Export Citation:
Assignee:
JAPAN SCIENCE & TECH CORP (JP)
DOI HIROFUMI (JP)
HIRAKI HIDEAKI (JP)
KANAI AKIO (JP)
DOI HIROFUMI (JP)
HIRAKI HIDEAKI (JP)
KANAI AKIO (JP)
International Classes:
C12N9/12; C12N15/10; (IPC1-7): C12N15/09; C12N9/12; C12N15/54; G06F17/00; G06F19/00
Foreign References:
| JPH08110909A | 1996-04-30 | |||
| JPH06266810A | 1994-09-22 |
Other References:
WEINBERG R. B.: "IDENTIFICATION OF FUNCTIONAL DOMAINS IN THE PLASMA APOLIPOPROTEINS BY ANALYSIS OF INTER-SPECIES SEQUENCE VARIABILITY.", JOURNAL OF LIPID RESEARCH, AMERICAN SOCIETY FOR BIOCHEMISTRY AND MOLECULAR BIOLOGY, INC., US, vol. 35., no. 12., 1 January 1994 (1994-01-01), US, pages 2212 - 2222., XP002912939, ISSN: 0022-2275
MATHUR E. J., ET AL.: "THE DNA POLYMERASE GENE FROM THE HYPERTHERMOPHILIC MARINE ARCHAEBACTERIUM, PYROCOCCUS FURIOSUS, SHOWS SEQUENCE HOMOLOGY WITH ALPHA-LIKE DNA POLYMERASES.", NUCLEIC ACIDS RESEARCH, INFORMATION RETRIEVAL LTD., vol. 19., no. 24., 1 January 1991 (1991-01-01), pages 6952., XP002912940, ISSN: 0305-1048
FORTERRE P.: "THE DNA POLYMERASE FROM THE ARCHAEBACTERIUM PYROCOCCUS FURIOSUS DOES NOT TESTIFY FOR A SPECIFIC RELATIONSHIP BETWEEN ARCHAEBACTERIAAND EUKARYOTES.", NUCLEIC ACIDS RESEARCH, INFORMATION RETRIEVAL LTD., vol. 20., no. 07., 1 January 1992 (1992-01-01), pages 1811., XP002912941, ISSN: 0305-1048
UEMORI T., ET AL.: "ORGANIZATION AND NUCLEOTIDE SEQUENCE OF THE DNA POLYMERASE GENE FROM THE ARCHAEON PYROCOCCUS FURIOSUS.", NUCLEIC ACIDS RESEARCH, INFORMATION RETRIEVAL LTD., vol. 21., no. 02., 1 January 1993 (1993-01-01), pages 259 - 265., XP002912942, ISSN: 0305-1048
See also references of EP 1013759A4
MATHUR E. J., ET AL.: "THE DNA POLYMERASE GENE FROM THE HYPERTHERMOPHILIC MARINE ARCHAEBACTERIUM, PYROCOCCUS FURIOSUS, SHOWS SEQUENCE HOMOLOGY WITH ALPHA-LIKE DNA POLYMERASES.", NUCLEIC ACIDS RESEARCH, INFORMATION RETRIEVAL LTD., vol. 19., no. 24., 1 January 1991 (1991-01-01), pages 6952., XP002912940, ISSN: 0305-1048
FORTERRE P.: "THE DNA POLYMERASE FROM THE ARCHAEBACTERIUM PYROCOCCUS FURIOSUS DOES NOT TESTIFY FOR A SPECIFIC RELATIONSHIP BETWEEN ARCHAEBACTERIAAND EUKARYOTES.", NUCLEIC ACIDS RESEARCH, INFORMATION RETRIEVAL LTD., vol. 20., no. 07., 1 January 1992 (1992-01-01), pages 1811., XP002912941, ISSN: 0305-1048
UEMORI T., ET AL.: "ORGANIZATION AND NUCLEOTIDE SEQUENCE OF THE DNA POLYMERASE GENE FROM THE ARCHAEON PYROCOCCUS FURIOSUS.", NUCLEIC ACIDS RESEARCH, INFORMATION RETRIEVAL LTD., vol. 21., no. 02., 1 January 1993 (1993-01-01), pages 259 - 265., XP002912942, ISSN: 0305-1048
See also references of EP 1013759A4
Attorney, Agent or Firm:
Nishizawa, Toshio (6F 37-10, Udagawa-ch, Shibuya-ku Tokyo, JP)
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