Title:
METHOD AND APPARATUS FOR RAPID DETERMINATION OF LIGAND-PROTEIN BINDING USING CHARCOAL ADSORPTION
Document Type and Number:
WIPO Patent Application WO2003015871
Kind Code:
A3
Abstract:
A method for evaluating binding of a ligand to a target protein. The method includes the steps of: (a) providing a sample comprising a target protein and a ligand, wherein the target protein and ligand are suspected to be bound reversibly together in a complex; (b) preconditioning activated charcoal with the target protein; (c) contacting the sample with the preconditioned activated charcoal for a time sufficient to allow for adsorption of unbound ligand to the activated charcoal; (d) eluting the sample from the activated charcoal; and (e) determining an amount of ligand in the eluted sample to thereby evaluate binding of the ligand to the target protein. An apparatus (Figure 1) useful in carrying out the method, and a method of making the same, are also disclosed.
Inventors:
POLLACK GARY M (US)
Application Number:
PCT/US2002/023556
Publication Date:
August 21, 2003
Filing Date:
July 25, 2002
Export Citation:
Assignee:
UNIV NORTH CAROLINA (US)
POLLACK GARY M (US)
POLLACK GARY M (US)
International Classes:
A61P1/00; G01N33/543; G01N33/551; (IPC1-7): G01N33/537; G01N33/538; A61L9/00
Foreign References:
| US5225158A | 1993-07-06 |
Other References:
WONG WILLIAM H.: "Determination of serum thyroxine after dissociation from thyroxine-binding globulin in alkaline solution and adsorption on dextran-coated charcoal", CLINICAL CHEMISTRY, vol. 21, no. 2, 1975, pages 216 - 220, XP002962219
HUWIG ET AL.: "Mycotoxin detoxication of animal feed by different adsorbents", TOXICOLOGY LETTERS, vol. 122, 30 April 2001 (2001-04-30), pages 179 - 188, XP002962220
TIGYI ET AL.: "A factor that activates oscillatory chloride currents in xenopus oocytes corpurifies with a subfraction of serum albumin", THE JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 266, no. 31, 5 November 1991 (1991-11-05), pages 20602 - 20609, XP002962221
KAYKATY ET AL.: "Determination of the synthetic steroid norgestomet in bovine plasma by capillary column gas chromatography/negative chemical ionization mass spectrometry", BIOMED. ENVIRON. MASS. SPECTROM., vol. 17, no. 2, August 1988 (1988-08-01), pages 121 - 126, XP002962222
HUWIG ET AL.: "Mycotoxin detoxication of animal feed by different adsorbents", TOXICOLOGY LETTERS, vol. 122, 30 April 2001 (2001-04-30), pages 179 - 188, XP002962220
TIGYI ET AL.: "A factor that activates oscillatory chloride currents in xenopus oocytes corpurifies with a subfraction of serum albumin", THE JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 266, no. 31, 5 November 1991 (1991-11-05), pages 20602 - 20609, XP002962221
KAYKATY ET AL.: "Determination of the synthetic steroid norgestomet in bovine plasma by capillary column gas chromatography/negative chemical ionization mass spectrometry", BIOMED. ENVIRON. MASS. SPECTROM., vol. 17, no. 2, August 1988 (1988-08-01), pages 121 - 126, XP002962222
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