Title:
METHOD AND ARRANGEMENT FOR ANALYZING SAMPLES
Document Type and Number:
WIPO Patent Application WO2003098313
Kind Code:
A3
Abstract:
The invention relates to a method and/or arrangement for analyzing fluorescent samples by means of an image-producing microscopic system, preferably a laser scanning microscope, according to which the sample is scanned spot by spot or line by line within at least one surface section, the radiation originating from the sample being split in a dispersing manner during scanning. The split radiation is detected according to the wavelength thereof by means of at least one range of detector elements. Two-dimensional or three-dimensional sample parts which correspond to or resemble prestored two-dimensional or three-dimensional geometric objects are selected by processing images based on the recorded and stored intensity distribution of at least one of said detector elements and/or at least one additional element detecting the radiation reflected by the sample. The spectral signature and/or spatial spectral sequence regarding the fluorescence markers which are arranged in the sample areas is/are analyzed for at least one part of said sample areas.
Inventors:
GERSTNER VOLKER (DE)
LINDENAU JOERG (DE)
LINDENAU JOERG (DE)
Application Number:
PCT/EP2003/005033
Publication Date:
July 15, 2004
Filing Date:
May 14, 2003
Export Citation:
Assignee:
ZEISS CARL JENA GMBH (DE)
GERSTNER VOLKER (DE)
LINDENAU JOERG (DE)
GERSTNER VOLKER (DE)
LINDENAU JOERG (DE)
International Classes:
G01N21/64; G02B21/00; (IPC1-7): G02B21/00; G01N21/64
Other References:
ANONYMOUS: "Cell Observer: Leben mit neuen Augen sehen", ZEISS BROCHURE, November 2000 (2000-11-01), pages 1 - 8, XP002257573, Retrieved from the Internet [retrieved on 20031014]
W. BÖCKER ET AL.: "Automated cell cycle analysis with fluorescence microscopy and image analysis", PHYS. MED. BIOL., 1996, pages 523 - 537, XP002257574, Retrieved from the Internet [retrieved on 20031014]
ANONYMOUS: "Particle Analyser: Schnelle Durchführung von Dokumentations- und Messaufgaben", ZEISS- NEWS, May 2002 (2002-05-01), pages 1 - 2, XP002257575, Retrieved from the Internet [retrieved on 20031014]
W. BÖCKER ET AL.: "Automated cell cycle analysis with fluorescence microscopy and image analysis", PHYS. MED. BIOL., 1996, pages 523 - 537, XP002257574, Retrieved from the Internet
ANONYMOUS: "Particle Analyser: Schnelle Durchführung von Dokumentations- und Messaufgaben", ZEISS- NEWS, May 2002 (2002-05-01), pages 1 - 2, XP002257575, Retrieved from the Internet
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