Title:
METHOD FOR ASSAYING CHOLESTEROL IN HIGH-DENSITY LIPOPROTEIN FRACTION AND ASSAY REAGENT KIT
Document Type and Number:
WIPO Patent Application WO/1997/000971
Kind Code:
A1
Abstract:
A method for assaying cholesterol in a high-density lipoprotein (HDL) fraction which comprises the following steps. By using an acylpolyoxyethylene sorbitan ester, cholesterol in the fractions of lipoproteins in a sample other than HDL is preferentially treated with enzymes such as cholesterol esterase and cholesterol oxidase to thereby form hydrogen peroxide. In the presence of peroxidases, colorless complexes are formed to thereby eliminate the hydrogen peroxide from the reaction system. Next, the actions of these enzymes on the cholesterol remaining in the fractions of lipoproteins other than HDL are suppressed by alkyl polyoxyethylene ethers. At the same time, the cholesterol contained in the HDL fraction is treated by these enzymes to thereby form hydrogen peroxide. The quinone coloring matter formed by the hydrogen peroxide is measured by the colorimetry to thereby determine the cholesterol content of the HDL fraction. By using this simplified method, a number of samples can be processed in a short time and even samples in a very small amount can be continuously assayed with an automatic analyzer. Thus it contributes to the improvement in the efficiency of routine work in clinical examinations.
Inventors:
IKEDA MASAFUMI (JP)
TABATA HIROMASA (JP)
KAKUYAMA TSUTOMU (JP)
HASHIGUCHI YOICHI (JP)
TABATA HIROMASA (JP)
KAKUYAMA TSUTOMU (JP)
HASHIGUCHI YOICHI (JP)
Application Number:
PCT/JP1996/001602
Publication Date:
January 09, 1997
Filing Date:
June 12, 1996
Export Citation:
Assignee:
INT REAGENTS CORP (JP)
IKEDA MASAFUMI (JP)
TABATA HIROMASA (JP)
KAKUYAMA TSUTOMU (JP)
HASHIGUCHI YOICHI (JP)
IKEDA MASAFUMI (JP)
TABATA HIROMASA (JP)
KAKUYAMA TSUTOMU (JP)
HASHIGUCHI YOICHI (JP)
International Classes:
G01N33/92; C12Q1/26; C12Q1/44; C12Q1/60; (IPC1-7): C12Q1/60; C12Q1/26; C12Q1/44
Other References:
CLINICAL TEST APPARATUS REAGENT, Vol. 18, No. 4, (1995), KEIKO MIYAMOTO et al., "Study of the Direct Measurement of HDL Cholesterol Using PEG Modifying Enzyme/Sulfuric Cyclodextrin Complex System", p. 613-620.
LIPID BIOCHEMISTRY STUDY, Vol. 37, (1995), HIROYUKI SUGIUCHI et al., "Basic Study of the Direct Measurement of HDL-Cholesterol", p. 219-222.
LIPID BIOCHEMISTRY STUDY, Vol. 37, (1995), HIROYUKI SUGIUCHI et al., "Basic Study of the Direct Measurement of HDL-Cholesterol", p. 219-222.
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