METHOD OF ASSAYING LYSOPHOSPHOLIPASE D ACTIVITY

Title:

METHOD OF ASSAYING LYSOPHOSPHOLIPASE D ACTIVITY

Document Type and Number:

WIPO Patent Application WO/2002/053569

Kind Code:

A1

Abstract:

Lysophospholipids represented by the following general formula (I): (I) wherein R¿1? and R¿2? are different from each other and one of them represents a saturated or unsaturated C¿8-22? fatty acid residue while the other represents hydrogen or acetyl; and X is a chromophore; a method of assaying lysophospholipase D activity characterized by using such a phospholipid as a substrate; a method of screening a substance inhibiting lysophospholipase D activity; and reagents and reagents kits useful in these methods. Use of these novel lysophospholipids as a substrate makes it possible to specifically assay lysophospholipase D activity at a high sensitivity. Moreover, use of a chromophore which is safe and can be easily detected makes it possible to assay a large number of samples quickly.

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Inventors:

KISHIMOTO TATSUYA (JP)
SODA YASUJI (JP)
MATSUYAMA YOSHIKO (JP)
ARAI HIROYUKI (JP)
AOKI JUNKEN (JP)

Application Number:

PCT/JP2001/011612

Publication Date:

July 11, 2002

Filing Date:

December 28, 2001

Export Citation:

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Assignee:

AZWELL INC (JP)
KISHIMOTO TATSUYA (JP)
SODA YASUJI (JP)
MATSUYAMA YOSHIKO (JP)
ARAI HIROYUKI (JP)
AOKI JUNKEN (JP)

International Classes:

C07F9/10; C07F9/12; C12N1/15; C12N1/19; C12N1/21; C12N9/16; C12N9/20; C12N15/55; C12Q1/44; (IPC1-7): C07F9/10; C07F9/12; C12N1/15; C12N1/19; C12N1/21; C12N5/10; C12N9/20; C12N15/55; C12N15/63; C12Q1/34; G01N33/15; G01N33/50

Domestic Patent References:

WO2000068386A1	2000-11-16
WO1995032221A2	1995-11-30

Foreign References:

US5011964A	1991-04-30
JP2002017398A	2002-01-22

Other References: