Title:
METHOD FOR ASSAYING MICRORNA, CANCER THERAPEUTIC AGENT, AND MEDICINAL COMPOSITION CONTAINING SAME FOR CANCER THERAPY
Document Type and Number:
WIPO Patent Application WO/2014/126233
Kind Code:
A1
Abstract:
The present invention addresses the problem of finding a microRNA that strongly relates to the stabilization of NRF2 in a tumor and providing a means of utilizing the same in diagnosing and treating cancer. As the results of a screening of 470 kinds of microRNAs in a microRNA library with the use of HeLa cells, 8 kinds of microRNAs, each showing a large decrease in the activity compared with a control, and 8 kinds of microRNAs, each showing a large increase in the activity, were specified. It was discovered that, by using the microRNAs thus specified, the activation of NRF2 in a living body, in particular a tumor cell, can be detected and thus the malignancy, etc. of a tumor can be distinguished. Also, it was discovered that a nucleic acid containing a microRNA sequence relating to a decrease in the ARE activity as described above is usable as a cancer therapeutic agent.
Inventors:
INAZAWA JOHJI (JP)
INOUE JUN (JP)
YAMAMOTO SHINSUKE (JP)
KAWANO TATSUYUKI (JP)
KOZAKI KEN-ICHI (JP)
INOUE JUN (JP)
YAMAMOTO SHINSUKE (JP)
KAWANO TATSUYUKI (JP)
KOZAKI KEN-ICHI (JP)
Application Number:
PCT/JP2014/053594
Publication Date:
August 21, 2014
Filing Date:
February 17, 2014
Export Citation:
Assignee:
NAT UNIV CORP TOKYO MED & DENT (JP)
International Classes:
C12Q1/68; A61K31/713; A61K33/243; A61K45/00; A61K48/00; A61P35/00; A61P43/00; C12N15/113
Domestic Patent References:
| WO2011111715A1 | 2011-09-15 | |||
| WO2009044899A1 | 2009-04-09 | |||
| WO2012121178A1 | 2012-09-13 | |||
| WO2009136501A1 | 2009-11-12 | |||
| WO2011125245A1 | 2011-10-13 |
Foreign References:
| JP2012184230A | 2012-09-27 | |||
| JP2008519606A | 2008-06-12 | |||
| JP2013028575A | 2013-02-07 |
Other References:
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MITSUISHI ET AL., CANCER CELL, vol. 22, 10 July 2012 (2012-07-10), pages 66 - 79, XP028404162
VOGLER ET AL., CANCER RES., vol. 69, no. 6, 15 March 2009 (2009-03-15), pages 2425 - 2434, XP055275886
ITOH, K. ET AL., BIOCHEM. BIOPH. RES CO., vol. 236, 1997, pages 313 - 322
URUNO, A.; MOTOHASHI, H.: "Nitric Oxide-Biol.", 2011, pages: 153 - 160
SINGH, A. ET AL., PLOS. MED., vol. 3, 2006, pages 1865 - 1876
THIMMULAPPA, R,K. ET AL., CANCER RESEARCH, vol. 62, 2002, pages 5196 - 5203
MITUISHI, Y. ET AL., CANCER CELL, vol. 22, 2012, pages 66 - 79
KIM, Y.R. ET AL., J. PATHOL., vol. 220, 2010, pages 446 - 451
TAGUCHI, K.; MOTOHASHI, H.; YAMAMOTO, GENES CELLS, vol. 16, 2011, pages 123 - 140
HAMMERMAN, P.S. ET AL., NEOPLASIA, vol. 13, 2011, pages 864 - 873
SHIBATA, T. ET AL., NEOPLASIA, vol. 13, 2011, pages 864 - 873
INAMI, Y. ET AL., J. CELL BIOL., vol. 193, 2011, pages 275 - 284
KOMATSU, M. ET AL., NAT. CELL BIOL., vol. 12, 2010, pages 213 - U217
TAGUCHI, K. ET AL., P. NATL. ACAD. SCI. USA, vol. 109, 2012, pages 13561 - 13566
LAU, A. ET AL., MOL. CELL BIOL., vol. 30, 2010, pages 3275 - 3285
JIANG, T. ET AL., CANCER RESEARCH, vol. 70, 2010, pages 5486 - 5496
SHIBATA, T. ET AL., P. NATL. ACAD. SCI. USA, vol. 105, 2008, pages 13568 - 13573
SOILS, L.M. ET AL., CLIN. CANCER RES., vol. 16, 2010, pages 3743 - 3753
AMBROS, V., NATURE, vol. 431, 2004, pages 350 - 355
ZHAO, Y.; SRIVASTAVA, D., TRENDS BIOCHEM. SCI., vol. 32, 2007, pages 189 - 197
KONG, Y.W.; FERLAND-MCCOLLOUGH, D.; JACKSON. T. J.; BUSHELL, M., LANCET, ONCOL., vol. 13, 2012, pages E249 - E258
LU, J. ET AL., NATURE, vol. 435, 2005, pages 834 - 838
MISKA, E.A., CURR OPIN. GENET. DEV., vol. 15, 2005, pages 563 - 568
SHIVDASANI, R.$., BLOOD, vol. 108, 2006, pages 3646 - 3653
ZHANG, B.H.; PAN, X.P.; COBB, G.P.; ANDERSON, T.A., DEV. BIOL., vol. 302, 2007, pages 1 - 12
LEWIS, B.P.; BURGE, C.B.; BARTEL, D.P., CELL, vol. 120, 2005, pages 15 - 20
TSURUTA, T. ET AL., CANCER RESEARCH, vol. 71, 2011, pages 6575 - 5778
TAKESHITA, F. ET AL., MOL. THER., vol. 18, 2010, pages 181 - 187
LIU, C. ET AL., NAT. MED., vol. 17, 2011, pages 211 - 215
D.A. BRAASCH; D.R. COREY, CHEM. BIOL., vol. 8, 2001, pages 1
M. KATAOKA; Y. HAYAKAWA, J. ORG. CHEM., vol. 64, 1999, pages 6087
T. WADA; F. HANDA; Y. SATO; S. KAWAHARA; M. SEKINE, NUCLEIC ACIDS SYMP. SER., vol. 37, 1997, pages 19
See also references of EP 2963125A4
MITSUISHI ET AL., CANCER CELL, vol. 22, 10 July 2012 (2012-07-10), pages 66 - 79, XP028404162
VOGLER ET AL., CANCER RES., vol. 69, no. 6, 15 March 2009 (2009-03-15), pages 2425 - 2434, XP055275886
ITOH, K. ET AL., BIOCHEM. BIOPH. RES CO., vol. 236, 1997, pages 313 - 322
URUNO, A.; MOTOHASHI, H.: "Nitric Oxide-Biol.", 2011, pages: 153 - 160
SINGH, A. ET AL., PLOS. MED., vol. 3, 2006, pages 1865 - 1876
THIMMULAPPA, R,K. ET AL., CANCER RESEARCH, vol. 62, 2002, pages 5196 - 5203
MITUISHI, Y. ET AL., CANCER CELL, vol. 22, 2012, pages 66 - 79
KIM, Y.R. ET AL., J. PATHOL., vol. 220, 2010, pages 446 - 451
TAGUCHI, K.; MOTOHASHI, H.; YAMAMOTO, GENES CELLS, vol. 16, 2011, pages 123 - 140
HAMMERMAN, P.S. ET AL., NEOPLASIA, vol. 13, 2011, pages 864 - 873
SHIBATA, T. ET AL., NEOPLASIA, vol. 13, 2011, pages 864 - 873
INAMI, Y. ET AL., J. CELL BIOL., vol. 193, 2011, pages 275 - 284
KOMATSU, M. ET AL., NAT. CELL BIOL., vol. 12, 2010, pages 213 - U217
TAGUCHI, K. ET AL., P. NATL. ACAD. SCI. USA, vol. 109, 2012, pages 13561 - 13566
LAU, A. ET AL., MOL. CELL BIOL., vol. 30, 2010, pages 3275 - 3285
JIANG, T. ET AL., CANCER RESEARCH, vol. 70, 2010, pages 5486 - 5496
SHIBATA, T. ET AL., P. NATL. ACAD. SCI. USA, vol. 105, 2008, pages 13568 - 13573
SOILS, L.M. ET AL., CLIN. CANCER RES., vol. 16, 2010, pages 3743 - 3753
AMBROS, V., NATURE, vol. 431, 2004, pages 350 - 355
ZHAO, Y.; SRIVASTAVA, D., TRENDS BIOCHEM. SCI., vol. 32, 2007, pages 189 - 197
KONG, Y.W.; FERLAND-MCCOLLOUGH, D.; JACKSON. T. J.; BUSHELL, M., LANCET, ONCOL., vol. 13, 2012, pages E249 - E258
LU, J. ET AL., NATURE, vol. 435, 2005, pages 834 - 838
MISKA, E.A., CURR OPIN. GENET. DEV., vol. 15, 2005, pages 563 - 568
SHIVDASANI, R.$., BLOOD, vol. 108, 2006, pages 3646 - 3653
ZHANG, B.H.; PAN, X.P.; COBB, G.P.; ANDERSON, T.A., DEV. BIOL., vol. 302, 2007, pages 1 - 12
LEWIS, B.P.; BURGE, C.B.; BARTEL, D.P., CELL, vol. 120, 2005, pages 15 - 20
TSURUTA, T. ET AL., CANCER RESEARCH, vol. 71, 2011, pages 6575 - 5778
TAKESHITA, F. ET AL., MOL. THER., vol. 18, 2010, pages 181 - 187
LIU, C. ET AL., NAT. MED., vol. 17, 2011, pages 211 - 215
D.A. BRAASCH; D.R. COREY, CHEM. BIOL., vol. 8, 2001, pages 1
M. KATAOKA; Y. HAYAKAWA, J. ORG. CHEM., vol. 64, 1999, pages 6087
T. WADA; F. HANDA; Y. SATO; S. KAWAHARA; M. SEKINE, NUCLEIC ACIDS SYMP. SER., vol. 37, 1997, pages 19
See also references of EP 2963125A4
Attorney, Agent or Firm:
SHIMURA MITSUHARU (JP)
Mitsuharu Shimura (JP)
Mitsuharu Shimura (JP)
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