Title:
METHOD FOR ASSESSING RISK OF DEVELOPING HEPATOCELLULAR CARCINOMA FROM NONALCOHOLIC STEATOHEPATITIS
Document Type and Number:
WIPO Patent Application WO/2021/117772
Kind Code:
A1
Abstract:
Provided is a method for assessing the risk of developing hepatocellular carcinoma from nonalcoholic steatohepatitis (NASH). A method for detecting hepatocytes having a risk of developing hepatocellular carcinoma or detecting tissue that contains said hepatocytes, wherein the method includes detecting the DNA methylation level of target CpG sites in genomic DNA derived from hepatocytes affected with NASH or from tissue that contains said hepatocytes, and detecting hepatocytes having the risk of developing hepatocellular carcinoma or tissue that contains said hepatocytes from the detected DNA methylation level.
Inventors:
KANAI YAE (JP)
ARAI ERI (JP)
KURAMOTO JUNKO (JP)
YOTANI TAKUYA (JP)
YAMADA YURIKO (JP)
ARAI ERI (JP)
KURAMOTO JUNKO (JP)
YOTANI TAKUYA (JP)
YAMADA YURIKO (JP)
Application Number:
PCT/JP2020/045879
Publication Date:
June 17, 2021
Filing Date:
December 09, 2020
Export Citation:
Assignee:
UNIV KEIO (JP)
SEKISUI MEDICAL CO LTD (JP)
SEKISUI MEDICAL CO LTD (JP)
International Classes:
C12Q1/6869
Domestic Patent References:
| WO2013168644A1 | 2013-11-14 | |||
| WO2015129916A1 | 2015-09-03 | |||
| WO2017038983A1 | 2017-03-09 | |||
| WO2020116573A1 | 2020-06-11 | |||
| WO2019181941A1 | 2019-09-26 | |||
| WO2019039532A1 | 2019-02-28 | |||
| WO2014136930A1 | 2014-09-12 | |||
| WO2012108516A1 | 2012-08-16 |
Foreign References:
| US20180135130A1 | 2018-05-17 |
Other References:
JIAYU, W. ET AL.: "Altered DNA Methylation Sites in Peripheral Blood Leukocytes from Patients with Simple Steatosis and Nonalcoholic Steatohepatitis (NASH)", MEDICAL SCIENCE MONITOR, vol. 24, 2018, pages 6946 - 6967, XP055835123
ZHANG, R.N . ET AL.: "Genome-wide analysis of DNA methylation in human peripheral leukocytes identifies potential biomarkers of nonalcoholic fatty liver disease", INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE, vol. 42, 2018, pages 443 - 452, XP055835124
DE MELLO, V.D. ET AL.: "Human liver epigenetic alterations in non-alcoholic steatohepatitis are related to insulin action", EPIGENETICS, vol. 12, no. 4, 2017, pages 287 - 295, XP055589771, DOI: 10.1080/15592294.2017.1294305
KURAMOTO, J. ET AL.: "Genome-wide DNA methylation analysis during non- alcoholic steatohepatitis-related multistage hepatocarcinogenesis: comparison with hepatitis virus-related carcinogenesis", CARCINOGENESIS, vol. 38, no. 3, 2017, pages 261 - 270, XP055835132
INT J ONCOL, vol. 43, 2013, pages 1333 - 1342
CANCER SCI, vol. 101, no. 1, 2010, pages 36 - 45
JPN J CAN RES, vol. 87, no. 12, 1996, pages 1210 - 1217
INT J CANCER, vol. 125, no. 12, 2009, pages 2854 - 2862
FRONT GENET, vol. 5, 2014, pages 24
CARCINOGENESIS, vol. 38, 2017, pages 261 - 270
J CANCER RES CLIN ONCOL, vol. 146, no. 10, 2020, pages 2461 - 2477
NUCLEIC ACIDS RES, vol. 22, 1994, pages 2990 - 7
ANAL BIOCHEM, vol. 10, 2000, pages 103 - 110
NAT PROTOC, vol. 3, 2008, pages 1903 - 8
MUTAT RES, vol. 573, 2005, pages 83 - 95
ZHANG, R.N . ET AL.: "Genome-wide analysis of DNA methylation in human peripheral leukocytes identifies potential biomarkers of nonalcoholic fatty liver disease", INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE, vol. 42, 2018, pages 443 - 452, XP055835124
DE MELLO, V.D. ET AL.: "Human liver epigenetic alterations in non-alcoholic steatohepatitis are related to insulin action", EPIGENETICS, vol. 12, no. 4, 2017, pages 287 - 295, XP055589771, DOI: 10.1080/15592294.2017.1294305
KURAMOTO, J. ET AL.: "Genome-wide DNA methylation analysis during non- alcoholic steatohepatitis-related multistage hepatocarcinogenesis: comparison with hepatitis virus-related carcinogenesis", CARCINOGENESIS, vol. 38, no. 3, 2017, pages 261 - 270, XP055835132
INT J ONCOL, vol. 43, 2013, pages 1333 - 1342
CANCER SCI, vol. 101, no. 1, 2010, pages 36 - 45
JPN J CAN RES, vol. 87, no. 12, 1996, pages 1210 - 1217
INT J CANCER, vol. 125, no. 12, 2009, pages 2854 - 2862
FRONT GENET, vol. 5, 2014, pages 24
CARCINOGENESIS, vol. 38, 2017, pages 261 - 270
J CANCER RES CLIN ONCOL, vol. 146, no. 10, 2020, pages 2461 - 2477
NUCLEIC ACIDS RES, vol. 22, 1994, pages 2990 - 7
ANAL BIOCHEM, vol. 10, 2000, pages 103 - 110
NAT PROTOC, vol. 3, 2008, pages 1903 - 8
MUTAT RES, vol. 573, 2005, pages 83 - 95
Attorney, Agent or Firm:
THE PATENT CORPORATE BODY ARUGA PATENT OFFICE (JP)
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