Title:
METHOD FOR BIOSYNTHESIS OF NMN
Document Type and Number:
WIPO Patent Application WO/2023/202281
Kind Code:
A1
Abstract:
The present invention relates to the technical field of bioengineering, and particularly, to a method for biosynthesis of NMN utilizing a genetically engineered bacterium. The genetically engineered bacterium is an Escherichia coli host with deletions of nicotinamide nucleotide amidase encoding gene pncC and nicotinamide mononucleotide adenylyltransferase gene nadR, absence of nicotinamide mononucleotide adenylyltransferase gene nadD expression and overexpression of NAD+ synthase encoding gene nadE, and/or overexpression of nicotinate phosphoribosyltransferase mutant encoding gene NadVmu, overexpression of ribose-phosphate diphosphokinase encoding gene Prs and overexpression of ribokinase encoding gene RbsK. By using the method for genetic transformation, functional genes for degrading nicotinamide-mononucleotide (NMN) in E. coli are knocked out, such that the generated NMN is unlikely to be degraded and efficiently reserved, thus elevating the maximum NMN yield to 2.88 g/L.
Inventors:
HUA JUN (CN)
PEI LIANG (CN)
PAN YUE (CN)
LIU WEI (CN)
LIU CHAO (CN)
WEI WANTAO (CN)
PEI LIANG (CN)
PAN YUE (CN)
LIU WEI (CN)
LIU CHAO (CN)
WEI WANTAO (CN)
Application Number:
PCT/CN2023/081819
Publication Date:
October 26, 2023
Filing Date:
March 16, 2023
Export Citation:
Assignee:
SICHUAN INGIA BIOSYNTHETIC CO LTD (CN)
International Classes:
C12N15/52; C12N1/21; C12N9/10; C12N15/54; C12N15/70; C12P19/30
Foreign References:
| CN114807078A | 2022-07-29 | |||
| US20220090027A1 | 2022-03-24 | |||
| CN110373397A | 2019-10-25 | |||
| CN111051520A | 2020-04-21 | |||
| CN113106080A | 2021-07-13 |
Other References:
LIMEI REN, WANG XIAORU; QI YONGHAO; HAN GUANGXIN; HAN TIANMIAO: "Research progress on function and synthesis of β-nicotinamide mononucleotide", SHENGWU ZIYUAN - AMINO ACIDS AND BIOTIC RESOURCES., WUHAN DAXUE, CN, vol. 43, no. 2, 9 April 2021 (2021-04-09), CN , pages 127 - 132, XP093098214, ISSN: 1006-8376, DOI: 10.14188/j.ajsh.2021.02.004
BLACK WILLIAM B., ASPACIO DEREK, BEVER DANIELLE, KING EDWARD, ZHANG LINYUE, LI HAN: "Metabolic engineering of Escherichia coli for optimized biosynthesis of nicotinamide mononucleotide, a noncanonical redox cofactor", MICROBIAL CELL FACTORIES, vol. 19, no. 1, 1 December 2020 (2020-12-01), pages 150, XP093098216, DOI: 10.1186/s12934-020-01415-z
BLACK WILLIAM B., ASPACIO DEREK, BEVER DANIELLE, KING EDWARD, ZHANG LINYUE, LI HAN: "Metabolic engineering of Escherichia coli for optimized biosynthesis of nicotinamide mononucleotide, a noncanonical redox cofactor", MICROBIAL CELL FACTORIES, vol. 19, no. 1, 1 December 2020 (2020-12-01), pages 150, XP093098216, DOI: 10.1186/s12934-020-01415-z
Attorney, Agent or Firm:
UNITALEN ATTORNEYS AT LAW CO., LTD. (CN)
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