Title:
METHOD FOR BREAKING NUCLEIC ACID AND ADDING ADAPTOR BY MEANS OF TRANSPOSASE, AND REAGENT
Document Type and Number:
WIPO Patent Application WO/2016/058127
Kind Code:
A1
Abstract:
Provided are a method for breaking a nucleic acid and adding an adaptor by means of a transposase, and a reagent. The method comprises the following steps: conducting random breaking of a nucleic acid by using a transposase-embedded complex, wherein the transposase-embedded complex comprises a transposase and a first adaptor comprising a transposase identification sequence, and two ends of the broken nucleic acid are separately connected to the first adaptor and are separately provided with a gap; by means of purification or chemical reagent treatment, eliminating the influence of the transposase in the system on a follow-up reaction; connecting to a second adaptor at the gap by using a ligase, wherein a sequence of the second adaptor is different from a sequence of the first adaptor; and conducting a PCR reaction by using primers targeted to and combined with the first adaptor and the second adaptor respectively, so as to obtain a product whose two ends are respectively connected to different adaptor sequences.
Inventors:
GENG CHUNYU (CN)
CHEN RUOYING (CN)
GUO RONGRONG (CN)
ALEXEEV ANDREI (US)
ZHANG YINGXIN (US)
JIANG HUI (CN)
ZHANG WENWEI (CN)
CHEN RUOYING (CN)
GUO RONGRONG (CN)
ALEXEEV ANDREI (US)
ZHANG YINGXIN (US)
JIANG HUI (CN)
ZHANG WENWEI (CN)
Application Number:
PCT/CN2014/088542
Publication Date:
April 21, 2016
Filing Date:
October 14, 2014
Export Citation:
Assignee:
BGI SHENZHEN CO LTD (CN)
International Classes:
C12N15/10; C12N15/11
Domestic Patent References:
WO2012103545A1 | 2012-08-02 | |||
WO2013131962A1 | 2013-09-12 |
Foreign References:
CN102264914A | 2011-11-30 | |||
CN102943074A | 2013-02-27 | |||
CN101125873A | 2008-02-20 | |||
CN1145641A | 1997-03-19 | |||
CN102703426A | 2012-10-03 | |||
CN103710323A | 2014-04-09 |
Other References:
See also references of EP 3208335A4
Attorney, Agent or Firm:
DHC IP ATTORNEYS (CN)
深圳鼎合诚知识产权代理有限公司 (CN)
深圳鼎合诚知识产权代理有限公司 (CN)
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