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Title:
METHOD FOR BREAKING NUCLEIC ACID AND ADDING ADAPTOR BY MEANS OF TRANSPOSASE, AND REAGENT
Document Type and Number:
WIPO Patent Application WO/2016/058127
Kind Code:
A1
Abstract:
Provided are a method for breaking a nucleic acid and adding an adaptor by means of a transposase, and a reagent. The method comprises the following steps: conducting random breaking of a nucleic acid by using a transposase-embedded complex, wherein the transposase-embedded complex comprises a transposase and a first adaptor comprising a transposase identification sequence, and two ends of the broken nucleic acid are separately connected to the first adaptor and are separately provided with a gap; by means of purification or chemical reagent treatment, eliminating the influence of the transposase in the system on a follow-up reaction; connecting to a second adaptor at the gap by using a ligase, wherein a sequence of the second adaptor is different from a sequence of the first adaptor; and conducting a PCR reaction by using primers targeted to and combined with the first adaptor and the second adaptor respectively, so as to obtain a product whose two ends are respectively connected to different adaptor sequences.

Inventors:
GENG CHUNYU (CN)
CHEN RUOYING (CN)
GUO RONGRONG (CN)
ALEXEEV ANDREI (US)
ZHANG YINGXIN (US)
JIANG HUI (CN)
ZHANG WENWEI (CN)
Application Number:
PCT/CN2014/088542
Publication Date:
April 21, 2016
Filing Date:
October 14, 2014
Export Citation:
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Assignee:
BGI SHENZHEN CO LTD (CN)
International Classes:
C12N15/10; C12N15/11
Domestic Patent References:
WO2012103545A12012-08-02
WO2013131962A12013-09-12
Foreign References:
CN102264914A2011-11-30
CN102943074A2013-02-27
CN101125873A2008-02-20
CN1145641A1997-03-19
CN102703426A2012-10-03
CN103710323A2014-04-09
Other References:
See also references of EP 3208335A4
Attorney, Agent or Firm:
DHC IP ATTORNEYS (CN)
深圳鼎合诚知识产权代理有限公司 (CN)
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