Title:
METHOD FOR CALCULATING DISSOCIATION CONSTANT IN SURFACE PLASMON RESONANCE ANALYSIS
Document Type and Number:
WIPO Patent Application WO/2006/022277
Kind Code:
A1
Abstract:
Calculation of the dissociation constant of a molecule interacting with a molecule to be analyzed and immobilized on a metal surface in surface plasmon resonance is realized by an analytic method providing highly reliable measurement results in which the noise level (the noise width of a reference chip) and the baseline variation (the signal variation of the reference chip) are low. In a method for measuring a signal variation of surface plasmon resonance using surface plasmon resonance measuring apparatus comprising a channel system including a cell formed on a metal film and a photodetecting means for detecting the state of surface plasmon resonance by measuring the intensity of a light beam totally reflected from the surface of the metal film in a state where the liquid flow is stopped after the liquid in the channel system is replaced, the dissociation constant of a molecule interacting with a molecule to be analyzed and immobilized on the metal surface is calculated from the results of the measurement of signal variation.
Inventors:
KURUMA KOJI (JP)
TSUZUKI HIROHIKO (JP)
TSUZUKI HIROHIKO (JP)
Application Number:
PCT/JP2005/015318
Publication Date:
March 02, 2006
Filing Date:
August 24, 2005
Export Citation:
Assignee:
FUJI PHOTO FILM CO LTD (JP)
KURUMA KOJI (JP)
TSUZUKI HIROHIKO (JP)
KURUMA KOJI (JP)
TSUZUKI HIROHIKO (JP)
International Classes:
G01N21/27; G01N21/05; G01N21/41; G01N33/543
Domestic Patent References:
| WO2003056296A2 | 2003-07-10 |
Foreign References:
| JP2000065731A | 2000-03-03 | |||
| US6713610B1 | 2004-03-30 | |||
| JPH06501546A | 1994-02-17 | |||
| JPH1038797A | 1998-02-13 | |||
| US20040072375A1 | 2004-04-15 |
Other References:
PEARCE KH ET AL: "Growth Hormone Binding Affinity for its Receptor Surpasses the Requirements for Cellular Activity.", BIOCHEMISTRY., vol. 38, 1999, pages 81 - 89, XP002993338
See also references of EP 1795886A4
See also references of EP 1795886A4
Attorney, Agent or Firm:
SIKs & Co. (Kyobashi-Nisshoku Bldg. 8-7, Kyobashi 1-chom, Chuo-ku Tokyo 31, JP)
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