Title:
METHOD CAPABLE OF BEING INDEPENDENTLY USED FOR PROTEIN RENATURATION OR CAPABLE OF BEING USED FOR PRECEDING OPERATIONS OF PROTEIN RENATURATION
Document Type and Number:
WIPO Patent Application WO/2016/201608
Kind Code:
A1
Abstract:
Provided is a method capable of being independently used for protein renaturation or capable of being used for preceding operations of the protein renaturation. The method comprises: (1) adding a sample containing protein to be subjected to renaturation, into a denaturing solution to dissolve the sample; (2) placing a container in a low-temperature environment, and enabling a denaturing agent to be gradually precipitated from the denaturing solution, so that a concentration reduction gradient of the denaturing agent is formed in the denaturing solution, the denaturation capability of the denaturing agent is gradually weakened, and renaturation protein and/or quasi-renaturation protein precursor is gradually formed in the denaturing solution; meanwhile, as the denaturing agent is crystallized and precipitated, the volume of the solution is reduced, and the concentration of the protein can be greatly improved; and (3) separating the renaturation protein and/or the quasi-renaturation protein precursor.
Inventors:
ZHANG PENG (CN)
Application Number:
PCT/CN2015/081480
Publication Date:
December 22, 2016
Filing Date:
June 15, 2015
Export Citation:
Assignee:
ZHANG PENG (CN)
International Classes:
C07K1/14
Domestic Patent References:
| WO2001055174A2 | 2001-08-02 |
Foreign References:
| CN101565448A | 2009-10-28 | |||
| CN1786017A | 2006-06-14 |
Other References:
EMILY J.G. ET AL.: "Probing the Protein-Folding Mechanism Using Denaturant and Temperature Effects on Rate Constants", PNAS, vol. 110, no. 42, 16 September 2013 (2013-09-16), pages 16784 - 16789, XP055337697
Attorney, Agent or Firm:
TSINGYIHUA INTELLECTUAL PROPERTY LLC (CN)
北京清亦华知识产权代理事务所（普通合伙） (CN)
北京清亦华知识产权代理事务所（普通合伙） (CN)
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