Title:
METHOD FOR CELL TRANSDUCTION AND MICROFLUIDIC CHIP FOR CELL TRANSDUCTION
Document Type and Number:
WIPO Patent Application WO/2022/211430
Kind Code:
A1
Abstract:
The present invention relates to a method for cell transduction and a microfluidic chip for cell transduction, wherein, by introducing traits by a microfluidic-based method, cellular stress induced by a strong centrifugal force can be avoided, and since a high multiplicity of infection (MOI) can be achieved even with a small volume, the present invention can be usefully employed in cell transformation.
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Inventors:
LEE JUNG MIN (KR)
Application Number:
PCT/KR2022/004358
Publication Date:
October 06, 2022
Filing Date:
March 28, 2022
Export Citation:
Assignee:
CTCELLS INC (KR)
International Classes:
C12N15/87; C12M1/12; C12M3/06; C12N5/0783
Foreign References:
| KR20150135669A | 2015-12-03 |
Other References:
ALEXANDER J. NEUMANN; JOSH SCHROEDER; MAURO ALINI; CHARLES W. ARCHER; MARTIN J. STODDART: "Enhanced Adenovirus Transduction of hMSCs Using 3D Hydrogel Cell Carriers", MOLECULAR BIOTECHNOLOGY ; PART B OF APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY, HUMANA PRESS INC, NEW YORK, vol. 53, no. 2, 1 March 2012 (2012-03-01), New York , pages 207 - 216, XP035153578, ISSN: 1559-0305, DOI: 10.1007/s12033-012-9522-y
LUNI CAMILLA; MICHIELIN FEDERICA; BARZON LUISA; CALABR VINCENZA; ELVASSORE NICOLA: "Stochastic Model-Assisted Development of Efficient Low-Dose Viral Transduction in Microfluidics", BIOPHYSICAL JOURNAL, ELSEVIER, AMSTERDAM, NL, vol. 104, no. 4, 19 February 2013 (2013-02-19), AMSTERDAM, NL, pages 934 - 942, XP028993787, ISSN: 0006-3495, DOI: 10.1016/j.bpj.2012.12.049
MADRIGAL JUSTIN L., STILHANO ROBERTA S., SILTANEN CHRISTIAN, TANAKA KIMBERLY, REZVANI SABAH N., MORGAN RYAN P., REVZIN ALEXANDER, : "Microfluidic generation of alginate microgels for the controlled delivery of lentivectors", JOURNAL OF MATERIALS CHEMISTRY. B, ROYAL SOCIETY OF CHEMISTRY, GB, vol. 4, no. 43, 1 January 2016 (2016-01-01), GB , pages 6989 - 6999, XP055972250, ISSN: 2050-750X, DOI: 10.1039/C6TB02150F
NAN LANG, ZHENYU YANG,HAO LYU,KITTY YU YEUNG LAU,HO CHEUNG SHUM: "A microfluidic system for one-chip harvesting of single-cell-laden hydrogels in culture medium", ADVANCED BIOSYSTEMS, vol. 3, no. 11, 5 June 2019 (2019-06-05), pages e1900076, XP055972252, DOI: 10.1002/adbi.201900076
LUNI CAMILLA; MICHIELIN FEDERICA; BARZON LUISA; CALABR VINCENZA; ELVASSORE NICOLA: "Stochastic Model-Assisted Development of Efficient Low-Dose Viral Transduction in Microfluidics", BIOPHYSICAL JOURNAL, ELSEVIER, AMSTERDAM, NL, vol. 104, no. 4, 19 February 2013 (2013-02-19), AMSTERDAM, NL, pages 934 - 942, XP028993787, ISSN: 0006-3495, DOI: 10.1016/j.bpj.2012.12.049
MADRIGAL JUSTIN L., STILHANO ROBERTA S., SILTANEN CHRISTIAN, TANAKA KIMBERLY, REZVANI SABAH N., MORGAN RYAN P., REVZIN ALEXANDER, : "Microfluidic generation of alginate microgels for the controlled delivery of lentivectors", JOURNAL OF MATERIALS CHEMISTRY. B, ROYAL SOCIETY OF CHEMISTRY, GB, vol. 4, no. 43, 1 January 2016 (2016-01-01), GB , pages 6989 - 6999, XP055972250, ISSN: 2050-750X, DOI: 10.1039/C6TB02150F
NAN LANG, ZHENYU YANG,HAO LYU,KITTY YU YEUNG LAU,HO CHEUNG SHUM: "A microfluidic system for one-chip harvesting of single-cell-laden hydrogels in culture medium", ADVANCED BIOSYSTEMS, vol. 3, no. 11, 5 June 2019 (2019-06-05), pages e1900076, XP055972252, DOI: 10.1002/adbi.201900076
Attorney, Agent or Firm:
SHIM, Kyoung-Shik et al. (KR)
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