METHOD FOR CLASSIFYING LEUKOCYTES AND REAGENTS

Title:

METHOD FOR CLASSIFYING LEUKOCYTES AND REAGENTS

Document Type and Number:

WIPO Patent Application WO/1988/009504

Kind Code:

A1

Abstract:

A reagent for classifying leukocytes, which enables classification and counting of leukocytes by lysing erythrocytes and acting on leukocytes and which contains at least one surfactant selected from the group consisting of (a) a first group of surfactants of polyoxyethylene anionic surfactants represented by the general formula: R1-R2-(CH2CH2O)n-X (wherein R1 represents alkyl, alkenyl or alkynyl having 10 to 22 carbon atoms, R2 represents O, (alpha) or COO, represents an integer of 8 to 30, and X represents SO3Na, COONa, OSO3Na or ONa) and (b) a second group of polyoxyethylene nonionic surfactants represented by the general formula: R1-R2-(CH2CH2O)n-H (wherein R1 represents alkyl, alkenyl or alkynyl having 10 to 22 carbon atoms, R2 represents O, (alpha) or COO, and n represents an integer of 8 to 30). A method of classifying leukocytes into three to five groupes of lymphocytes, monocytes, and granulocytes according to particle analysis methods, such as the RF method or the DC method, using said reagent is also disclosed. The reagent may contain an osmotic pressure increasing agent and a solubilizing agent. This method enables classification of leukocytes into 3 to 5 types and differentiation of normal cells from abnormal cells.

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Inventors:

HAMAGUCHI YUKIO (JP)
ITO KENJI (JP)
TSUJINO YUKIO (JP)
MORIYAMA KAZUHIRO (JP)
TAKENAKA IKUYA (JP)
MORIKAWA TAKASHI (JP)
OHMI HITOMI (JP)

Application Number:

PCT/JP1988/000514

Publication Date:

December 01, 1988

Filing Date:

May 27, 1988

Export Citation:

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Assignee:

TOA MEDICAL ELECTRONICS (JP)

International Classes:

H01H11/00; G01N33/48; G01N33/49; G01N33/50; H01H15/04; (IPC1-7): G01N33/48; G01N33/49

Foreign References:

JPH06271857A	1994-09-27
JPH06179163A	1994-06-28
JPH0673356A	1994-03-15

Other References: