METHOD FOR COLLECTION OF NUCLEATED RED BLOOD CELLS BY DENSITY-GRADIENT CENTRIFUGATION UTILIZING CHANGE IN DENSITY OF BLOOD CELLS

WIPO Patent Application WO/2012/023298

A1

Provided is a method for concentrating and collecting fetus-derived nucleated red blood cells contained in a small amount in the blood of a mother body. A method for concentrating and collecting nucleated red blood cells from the blood of a mother body, comprising (i) subjecting the blood of the mother body to first density-gradient centrifugation to collect a cell fraction containing the nucleated red blood cells, (ii) treating the cell fraction containing the nucleated red blood cells in such a manner that the density of the nucleated red blood cells can be varied in a selective manner, thereby avoiding the overlapping of the density of the nucleated red blood cells and the density of white blood cells each other, and (iii) subjecting the treated cell fraction containing the nucleated red blood cells to second density-gradient centrifugation to collect a fraction containing the nucleated red blood cells.

TAKAMURA YUZURU (JP)
IDEGAMI KOTARO (JP)
KOGI MIEKO (JP)
TAKABAYASHI HARUO (JP)

PCT/JP2011/054414

February 23, 2012

February 21, 2011

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JAPAN ADV INST SCIENCE & TECH (JP)
KANAZAWA INST OF TECHNOLOGY (JP)
KANAZAWA MEDICAL UNIVERSITY (JP)
TAKAMURA YUZURU (JP)
IDEGAMI KOTARO (JP)
KOGI MIEKO (JP)
TAKABAYASHI HARUO (JP)

G01N33/48; C12M1/00; C12N5/078; C12Q1/02; C12Q1/68; G01N1/10; G01N1/28; G01N1/34; G01N33/50

WO2003050532A1

2003-06-19

JPH09509312A	1997-09-22
JPH11502106A	1999-02-23

HIRAKI, Yusuke et al. (JP)
Yusuke Hiraki (JP)

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