Title:
METHOD AND COMPOSITION FOR PRODUCING TARGET NUCLEIC ACID MOLECULE
Document Type and Number:
WIPO Patent Application WO/2018/151574
Kind Code:
A1
Abstract:
The present invention provides a method for producing a target nucleic acid molecule, comprising the steps of: (a) providing a target sequence region, a first flanking sequence region linked to the 5' end of the target sequence region and containing at least one deaminated base, and a double strand nucleic acid molecule including a second flanking sequence region linked to the 3' end of the target sequence region; and (b) incubating the nucleic acid molecule and the deaminated base-specific endonuclease to remove the first flanking sequence region ranging from the deaminated base closest to the 5' end of the target sequence region and to the 5' end of the nucleic acid molecule. Provided is also a composition for producing a target nucleic acid molecule, comprising the double-strand nucleic acid molecule and the deaminated base-specific endonuclease.
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Inventors:
KWON SUNG HOON (KR)
KIM JUNG MIN (KR)
NOH JIN SUNG (KR)
YEOM HUI RAN (KR)
RYU TAE HOON (KR)
KIM JUNG MIN (KR)
NOH JIN SUNG (KR)
YEOM HUI RAN (KR)
RYU TAE HOON (KR)
Application Number:
PCT/KR2018/002049
Publication Date:
August 23, 2018
Filing Date:
February 20, 2018
Export Citation:
Assignee:
CELEMICS INC (KR)
SEOUL NAT UNIV R&DB FOUNDATION (KR)
SEOUL NAT UNIV R&DB FOUNDATION (KR)
International Classes:
C12Q1/6806
Domestic Patent References:
| WO2015120177A1 | 2015-08-13 | |||
| WO2014012107A2 | 2014-01-16 |
Foreign References:
| JP2015521468A | 2015-07-30 | |||
| US20140274736A1 | 2014-09-18 | |||
| US20140303000A1 | 2014-10-09 |
Attorney, Agent or Firm:
KWAK, Hyun Kyu (KR)
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