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Patent Searching and Data


Title:
METHOD AND COMPOSITION FOR REDUCING CORE FUCOSYLATION OF ANTIBODY
Document Type and Number:
WIPO Patent Application WO/2019/196697
Kind Code:
A1
Abstract:
A small molecule mannose analog for the manufacture of a recombinant antibody having complex N-linked glycans but reduced core fucosylation. Said mannose analog is typically taken in by host cells (e.g. by means of active transport or passive diffusion), inhibiting the production of GDP fucose. As mannose is being used as the synthetic substrate, the cost is extremely low. Moreover, by using said method, the core fucosylation will be reduced while the high mannose glycoform ratio of N-linked glycans will not be affected.

Inventors:
ZHANG YING (CN)
XIAO ZHIHUA (CN)
Application Number:
PCT/CN2019/080902
Publication Date:
October 17, 2019
Filing Date:
April 02, 2019
Export Citation:
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Assignee:
SHANGHAI HAOZHE INFORMATION TECH LIMITED (CN)
International Classes:
C12N5/071; C07H13/04
Foreign References:
CN102076865A2011-05-25
Other References:
GAN, LUTANG, CHINESE ENCYCLOPEDIA CHEMISTRY (NON-OFFICIAL TRANSLATION), 28 February 1989 (1989-02-28), pages 296
LAFONT D. ET. AL.: "Syntheses of L-glucosamine donors for 1, 2-trans-glycosylation reactions", TETRAHEDRON : ASYMMETRY, vol. 17, no. 24, 27 December 2006 (2006-12-27), XP024962250
LI C.: "''Designer A 1,6-Fucosidase Mutants Enable Direct Core Fucosylation of Intact N-Glycopeptides and N-Glycoproteins", J. AM. CHEM. SOC.,, vol. 139, no. 42, 9 October 2017 (2017-10-09), pages 15074 - 15087, XP055642098, Retrieved from the Internet
HARUE I. N.: "Double knockdown of a 1, 6-fucosyltransferase (FUT8) and GDP- mannose 4, 6-dehydratase (GMD) in antibody-producing cells:a new strategy for generating fully non-fucosylated therapeutic antibodies with enhanced ADCC", BMC BIOTECHNOLOGY, 30 November 2007 (2007-11-30), XP002475274
FERRARA C .: "Unique carbohydrate - carbohydrate interactions are required for high affinity binding between FcyRIII and antibodies lacking core fuco- se", PNAS, 2 August 2011 (2011-08-02), XP055206253
Attorney, Agent or Firm:
TUO YING LAW OFFICES (SHANGHAI) (CN)
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