This application is being filed on 20 August 2013, as a PCT International Patent application and claims priority to U.S. Patent Application Serial No.

61/691,117 filed on 20 August 2012, the disclosure of which is incorporated herein by reference in its entirety.

Field of the Invention

The present invention relates to the field of biomedicine and specifically methods useful for cancer therapy. In particular, embodiments of the invention relate to methods and compositions for carrying out cellular immunotherapy comprising T cells modified with tumor targeting receptors.

Statement Regarding Federally Sponsored Research

This invention was made with government support in the fonn of grants from the United States Department of Health and Human Services and from the Leukemia and Lymphoma Society. The United States government has certain rights in the invention.

Background of the Invention

The adoptive transfer of human T lymphocytes that are engineered by gene transfer to express chimeric antigen receptors (chimeric receptors) specific for surface molecules expressed on tumor cells has the potential to effectively treat advanced malignancies. Chimeric receptors are synthetic receptors that include an extracellular ligand binding domain, most commonly a single chain variable fragment of a monoclonal antibody (scFv) linked to intracellular signaling components, most commonly CD3ζ alone or combined with one or more costimulatory domains. Much of the research in the design of chimeric receptors has focused on defining scFvs and other ligand binding elements that target malignant cells without causing serious toxicity to essential normal tissues, and on defining the optimal composition of intracellular signaling modules to activate T cell effector functions. However, it is uncertain whether the variations in chimeric receptor design that mediate superior in vitro function will translate reproducibly into improved in vivo therapeutic activity in clinical applications of chimeric receptor- modified T cells.

There is a need to identify methods for determining elements of chimeric receptor design that are important for therapeutic activity and cell populations to genetically modify and adoptively transfer that provide enhanced survival and efficacy in vivo.

Summary of the Invention

In one aspect, the present disclosure relates to methods and compositions to confer and/or augment immune responses mediated by cellular immunotherapy, such as by adoptively transferring tumor-specific, genetically modified subsets of CD8+ or CD4+ T cells alone, or in combination. The disclosure provides for chimeric receptor nucleic acids, and vectors and host cells including such nucleic acids. The nucleic acid sequence that encodes the chimeric receptor links together a number of modular components that can be excised and replaced with other components in order to customize the chimeric receptor for efficient T cell activation and recognition of a specific target molecule or an epitope on the target molecule.

Tn embodiments, a chimeric receptor nucleic acid comprises a

polynucleotide coding for a ligand binding domain, wherein the ligand is a molecule expressed on malignant or infected cells, a polynucleotide coding for a polypeptide spacer wherein the polypeptide spacer is about 200 amino acids or less, a polynucleotide coding for a transmembrane domain; and a polynucleotide coding for intracellular signaling domains. In embodiments, the polypeptide spacer comprises a modified IgG4 hinge region containing an amino acid sequence X ₁ PPX ₂ P that may be linked to other amino acid sequences including but not limited to the CH2 and CH3 or CH3 only sequences of the Ig Fc. It has been surprisingly found that the length of the spacer region that is presumed not to have signaling capability affects the in vivo efficacy of the T cells modified to express the chimeric receptor and needs to be customized for individual target molecules for optimal tumor or target cell recognition.

Another aspect of the disclosure provides an isolated chimeric receptor nucleic acid comprising: a polynucleotide coding for a ligand binding domain, wherein the ligand is a tumor specific antigen, viral antigen, or any other molecule expressed on a target cell population that is suitable to mediate recognition and elimination by a lymphocyte; a polynucleotide coding for a polypeptide spacer wherein the polypeptide spacer is of a customized length that is specific for each targeted ligand, wherein the spacer provides for enhanced T cell proliferation and/ or cytokine production as compared to a reference chimeric receptor; a

polynucleotide coding for a transmembrane domain; and a polynucleotide coding for one or more intracellular signaling domains. In embodiments, a long spacer is employed if the epitope on the target ligand is in a membrane proximal position and a short spacer is employed if the epitope on the target ligand is in a membrane distal position. The disclosure includes expression vectors and host cells comprising the isolated chimeric receptor as described herein.

Another aspect of the disclosure provides a chimeric receptor polypeptide comprising a ligand binding domain, wherein the ligand is a tumor specific antigen, viral antigen or any other molecule that is expressed on a target cell population and can be targeted to mediate recognition and elimination by lymphocytes; a polypeptide spacer wherein the polypeptide spacer is about 10-229 amino acids; a transmembrane domain; and one or more intracellular signaling domains. In embodiments, the polypeptide spacer comprises a modified IgG hinge region containing the amino acid sequence X1PPX2P.

In another aspect, the present disclosure provides compositions to confer and/or augment immune responses mediated by cellular immunotherapy, such as by adoptively transferring tumor-specific, subset specific genetically modified CD4+ T cells, wherein the CD4+ T cells confer and/or augment the ability of CD8+ T cells to sustain anti-tumor reactivity and increase and/or maximize turn or- specific proliferation. In embodiments, the CD4+ cells are genetically modified to express a chimeric receptor nucleic acid and/or chimeric receptor polypeptide as described herein.

In another aspect, the present disclosure provides compositions to confer and/or augment immune responses mediated by cellular immunotherapy, such as by adoptively transferring tumor-specific, subset specific genetically modified CD8+ T cells. In embodiments, the CD8-i- cells express a chimeric receptor nucleic acid and/or chimeric receptor polypeptide as described herein. In another embodiment, the present invention provides an adoptive cellular immunotherapy composition having a genetically modified CD8+ cytotoxic T lymphocyte cell preparation to confer and/or augment immune responses, wherein the cytotoxic T lymphocyte cell preparation comprises CD8+ T cells that express a chimeric receptor comprising a ligand binding domain for a ligand associated with the disease or disorder, a customized spacer region, a transmembrane domain; and an intracellular signaling domain of a T cell or other receptors, such as a costimulatory domain, and/or a genetically modified helper T lymphocyte cell preparation, wherein the helper T lymphocyte cell preparation has CD4+ T cells that express a chimeric receptor comprising an antibody variable domain specific for the ligand associated with the disease or disorder, a customized spacer region, a transmembrane domain; and one or more intracellular signaling domains.

In one embodiment, the present invention provides a method of performing cellular immunotherapy in a subject having a disease or disorder by administering to the subject a genetically modified cytotoxic T lymphocyte cell preparation that provides a cellular immune response, wherein the cytotoxic T lymphocyte cell preparation comprises CD8+ T cells that have a chimeric receptor comprising a polynucleotide coding for a ligand binding domain, wherein the ligand is a tumor specific antigen, viral antigen, or any other molecule expressed on a target cell population that is suitable to mediate recognition and elimination by a lymphocyte; a polynucleotide coding for a polypeptide spacer wherein the polypeptide spacer is of a customized length that is specific for each targeted ligand, wherein the spacer provides for enhanced T cell proliferation and/or cytokine production as compared to a reference chimeric receptor; a polynucleotide coding for a transmembrane domain; and a polynucleotide coding for one or more intracellular signaling domains. In embodiment, the ligand binding domain is an extracellular antibody variable domain specific for a ligand associated with the disease or disorder. An embodiment includes a genetically modified helper T lymphocyte cell preparation that wherein the helper T lymphocyte cell preparation comprises CD4+ T cells that have a chimeric receptor comprising an a polynucleotide coding for a ligand binding domain, wherein the ligand is a tumor specific antigen, viral antigen, or any other molecule expressed on a target cell population that is suitable to mediate recognition and elimination by a lymphocyte; a polynucleotide coding for a polypeptide spacer wherein the polypeptide spacer is of a customized length that is specific for each targeted Hgand, wherein the spacer provides for enhanced T cell proliferation and/or cytokine production as compared to a reference chimeric receptor; a polynucleotide coding for a transmembrane domain; and a polynucleotide coding for one or more intracellular signaling domains. In embodiments, the genetically modified CD8+ and genetically modified CD4+ cell population are coadministered. In embodiments, the T cells are autologous or allogeneic T cells.

Various modifications of the above method are possible. For example, the chimeric receptor that is expressed by the CD4+ T cell and the CD8+ T cell can be the same or different.

In another aspect, the present invention provides a method of manufacturing an adoptive immunotherapy composition by obtaining a chimeric receptor modified tumor-specific CD8+ cytotoxic T lymphocyte cell preparation that elicits a cellular immune response and expresses an antigen-reactive chimeric receptor, wherein the modified cytotoxic T lymphocyte cell preparation comprises CD8+ T cells that have a chimeric receptor comprising a ligand binding domain, wherein the ligand is a tumor specific antigen, viral antigen, or any other molecule expressed on a target cell population that is suitable to mediate recognition and elimination by a lymphocyte; a polypeptide spacer wherein the polypeptide spacer is of a customized length that is specific for each targeted ligand, wherein the spacer provides for enhanced T cell proliferation and/or cytokine production as compared to a reference chimeric receptor; a transmembrane domain; and one or more intracellular signaling domains.; and/or obtaining a modified na'ive or memory CD4+ T helper cell wherein the modified helper T lymphocyte cell preparation comprises CD4+ cells that have a chimeric receptor comprising a ligand binding domain, wherein the ligand is a tumor specific antigen, viral antigen, or any other molecule expressed on a target cell population that is suitable to mediate recognition and elimination by a lymphocyte; a polypeptide spacer wherein the polypeptide spacer is of a customized length that is specific for each targeted ligand, wherein the spacer provides for enhanced T cell proliferation and/or cytokine production as compared to a reference chimeric receptor; a transmembrane domain; and one or more intracellular signaling domains. These and other embodiments of the invention are described further in the accompanying specification, drawings and claims.

Brief Description of the Drawings

Figure 1 Library of spacer sequences. We constructed a plasmid library that contain codon optimized D A sequences that encode extracellular components including of the IgG4 hinge alone, IgG4 hinge linked to CH2 and CH3 domains, or IgG4 hinge linked to the CH3 domain. Any scFV sequence (VH and VL) can be cloned 5' to the sequences encoding this library of variable spacer domains. The spacer domains are in turn linked to CD28 transmembrane and intracellular signaling domains and to CD3 zeta. A T2A sequence in the vector separates the chimeric receptor from a selectable marker encoding a truncated human epidermal growth factor receptor (tEGFR).

Figure 2: In vitro cytotoxicity, cytokine production, and proliferation of T-cells modified to express 2A2 RORl chimeric receptors with modified spacer length. (A) Phenotype of purified CD8 ⁺ T _C M~derived cell lines modified with each of the 2A2 RORl chimeric receptors with long, intermediate and short spacer domain. Staining with anti-F(ab) antibody that binds to an epitope in the 2A2 scFV shows surface expression of RORl chimeric receptors with full length or truncated spacer. (B) Cytolytic activity of T-ceils expressing the various 2A2 RORl chimeric receptors with long (·), intermediate (A) and short spacer (♦), or a tEGFR control lentiviral vector against RORl ¹ (x) and control target cells. The bar diagram summarizes cytotoxicity data from 3 independent experiments (E:T = 30:1) normalized to cytolytic activity by 2A2 RORl chimeric receptor 'long' = 1 , and analyzed by Student's t-test. (C) CFSE dye dilution was used to measure proliferation of 2A2 RORl chimeric receptor and tEGFR control T-cells, 72 hours after stimulation with Raji/ROR l (left panel) and primary CLL cells (right panel) without addition of exogenous cytokines. For analysis, triplicate wells were pooled and the proliferation of live (ΡΓ), CDS' T-celis analyzed. Numbers above each histogram indicate the number of cell divisions the proliferating subset underwent, and the fraction of T-cells in each gate that underwent >4/3/2/l cell divisions is provided next to each plot. (D) Multiplex cytokine assay of supematants obtained after 24 hours from triplicate co-cultures of 5x10 ⁴ T-cells expressing the various 2A2 RORl chimeric receptors with Raji/RORl and primary CLL cells. Multiplex cytokine data from 3 independent experiments were normalized (cytokine release by 2A2 RORl chimeric receptor 'long' = 1 ) and analyzed by Student's t-test (right bar diagram).

Figure 3. RU chimeric receptor requires a long spacer for recognition of

RORl ⁺ tumor cells. The sequences encoding the scFV from the Rl 1 monoclonal antibody that is specific for an epitope in the membrane proximal ringle domain of the orphan tyrosine kinase receptor RORl were cloned upstream of lgG4 hinge only (short), IgG4 hinge/CH3 (intermediate), and lgG4 hinge/CH2/CH3 sequences in our chimeric receptor library containing the 4-1 BB costimulatory domains and prepared as ientiviral vectors. A). Human CD8 ⁺ T cells were transduced and the transduction efficiency with each of the short, intermediate and long chimeric receptors was determined by staining for the tEGFR marker. B). Transduced T cells expressing the short (top), intermediate (middle), and long (bottom) were assayed for lysis of K562 leukemia cells alone or transfected to express RORl . Only the T cells expressing the long spacer chimeric receptor efficiently killed ROR1+ K562 cells. C).

Transduced T cells expressing the short (top), intermediate (middle), and long (bottom) were labeled with CFSE, stimulated with K562 cells expressing ROR l or CD19 (control) and assayed for cell proliferation over 72 hours. The T cells expressing the long spacer chimeric receptor proliferated specifically to the RORl + K562 cells. D). Transduced T cells expressing the short (top), intermediate (middle), and long (bottom) were stimulated with Raji lymphoma cells and K562 cells that expressed RORl or CD19 (control) and assayed for the secretion of interferon gamma into the supernatant over 24 hours. The T cells expressing the long spacer chimeric receptor proliferated and produced the highest levels of interferon gamma in response to RORl positive target cells.

Figure 4: Design of RORl chimeric receptors with modified spacer length and derived from the 2A2 and R12 scFV with different affinity. (A) Design of Ientiviral transgene inserts encoding a panel of RORl chimeric receptors containing the 2A2 scFV, an lgG4-Fc derived spacer of 'Hinge-CH2-CH3 ' (long spacer, 229 AA), 'Hinge-CT13 ' (intermediate, 1 1 9 AA), or 'Hinge' only (short, 12 AA), and a signal ing module with ΟΏ3ζ and CD28. Each chimeric receptor cassette contains a truncated EGFR marker encoded downstream of a T2A element. (B) Lentiviral transgene inserts encoding ROR1 -specific chimeric receptors derived from the R12 and 2A2 scFV with short IgG4-Fc 'Hinge' spacer (12 AA), and a signaling module containing CD28 or 4-1BB and ΟΌ3ζ respectively (total: 4 constructs).

Figure S: Anti-tumor reactivity of T-cells modified with ROR1 chimeric receptors derived from mAb R12 with higher affinity than 2A2. (A) tEGFR expression on purified polyclonal CD8 ⁺ Tc _M -derived T-cell lines modified with each of the R12 and 2A2 ROR1 chimeric receptors with short IgG4-Fc 'Hinge' spacer, and CD28 or 4-1BB costimulatory domain. (B) Cytotoxicity against RORl ⁺ and control target cells by T-cells expressing Rl 2(28- ; 4- 1 ΒΒ-Δ) and 2 A2 RO 1 chimeric receptors (28-·; 4-lBBo) or a tEGFR control vector (x). (C) Multiplex cytokine assay of supernatants obtained after 24 hours from co-cultures of 5x10 ⁴ T- cells expressing the various ROR1 chimeric receptors with Raji/RORl tumor cells. The middle/right bar diagrams show normalized multiplex data from 3 independeot experiments (cytokine release by ROR1 chimeric receptor 2A2 = 1) analyzed by Student's t-test. (D) Proliferation of ROR1 chimeric receptor T-cells and tEGFR control T-cells 72 hours after stimulation with Raji/RORl cells and without addition of exogenous cytokines was assessed by CFSE dye dilution. Numbers above each histogram indicate the number of cell divisions the proliferating subset underwent, and the fraction of T-cells in each gate that underwent >4/3/2/l cell divisions is provided above each plot.

Figure 6: Analysis of cytokine production and proliferation of CD4 ⁺ T- cells lines modified with a ROR1 chimeric receptor derived from mAb R12 with higher affinity than 2A2. (A-B) The 2A2 and R12 ROR1 chimeric receptors had the short spacer and a CD28 costimulatory domain. (A) Multiplex cytokine analysis from supernatants obtained 24 hours after stimulation of 5x10 ⁴ CD4 ^! T- cells expressing the 2A2 and R12 ROR1 chimeric receptor with Raji ROR l tumor cells. (B) Proliferation of CD4 ⁺ R12 and 2A2 ROR1 chimeric receptor T-cells and tEGFR control T-cells 72 hours after stimulation with Raji/RORl cells and without addition of exogenous cytokines was assessed by CFSE dye dilution. Numbers above each histogram indicate the number of cell divisions the proliferating subset underwent, and the fraction of T-cells in each gate that underwent >5/4/3/2/l cell divisions is provided above the histograms. Figure 7: Recognition of primary CLL by T-cells modified with 2A2 and R12 RORl chimeric receptors with optimal short spacer and 4-1BB

costimulatory domain or with a CD19-specific chimeric receptor. (A)

Expression of ROR1/CD19 on primary CLL, and CD80/86 on primary CLL and Raji RORl tumor cells (black dot plots) that can engage CD28 on chimeric receptor T-cells (white histograms). Staining with matched isotype control mAbs is shown as grey dot plots/histograms. (B) Cytolytic activity of T-cells expressing the 2Α2(·) and R12 RORl chimeric receptor (■), a CD19-specific chimeric receptor (A) and T-cells modified with a tEGFR control vector (x) against primary CLL (left diagram) and normal B cells (right diagram) analyzed by chromium release assay. Cytotoxicity data against primary CLL from 4 independent experiments (E:T = 30: 1 ) were normalized (cytolytic activity by RORl chimeric receptor 2A2 = 1) and analyzed by Student's t-test (bar diagram). (C) Multiplex cytokine analysis after a 24-hour stimulation of 5xl0 ⁴ chimeric receptor T-cells with primary CLL cells. Cytokine release of unstimulated chimeric receptor T-cells was below 3.6 pg/ml (detection limit) (left bar diagram). ELISA for TFN-γ production by 5x10 ⁴ 2A2 and R12 RORl chimeric receptor T-cells after a 24-hour co-culture with primary CLL. O.D. of 1 corresponds to approximately 250 pg/ml (right bar diagram). (D)

Proliferation of CD8 ⁺ T-cells modified with the 2A2 RORl , R12 RORl and a CD19 chimeric receptor, 72 hours after stimulation with primary CLL cells. Numbers above each histogram indicate the number of cell divisions, and the fraction of T- cells in each gate that underwent >3/2/l cell divisions is provided next to each plot.

Figure 8: The function of RORl-chimeric receptor and CD19-chimeric receptor modified CD8 ⁺ T-cells against primary CLL is augmented by chimeric receptor-modified CD4 ⁺ helper T-cells. (A) ELISA for 1L-2 production from triplicate co-cultures of 5xl0 ⁴ CD8 ⁺ and CD4 ⁺ T-cells expressing the R12 RORl and CD19-chimeric receptor respectively, incubated with primary CLL for 24-hours. O.D. of 1 corresponds to approx. 800 pg/ml. (B) Proliferation of chimeric receptor- modified CD8 ⁺ T-cells in response to primary CLL is enhanced by addition of chimeric receptor-mod ified CD4 T-cells. CFSE-labeled CD8 T-cells expressing the 2A2 RORl , R 12 RORl and CD19-chimeric receptor respectively, were co- cultured with tumor cells and with 2A2 RORl , R12 RORl and CD19-chimeric receptor transduced or control unt anduced CD4 ⁺ T-cells (CD8 ⁺ :CD4 = 1 : 1 ). Proliferation of the CD8 subset was analyzed 72 hours after stimulation. Numbers above each histogram indicate the number of cell divisions, and the fraction of T- cells in each gate that underwent >3/2/l cell divisions is provided above each plot.

Figure 9: In vivo anti-tumor efficacy of 2A2 ROR1, R12 ROR1 and CD19 chimeric receptor T-cells. Cohorts of mice were inoculated with 0.5x10 ⁶ JeKo-l/ffluc MCL via tail vein injection, and 5x10 ⁶ 2A2 ROR1, R12 ROR1 or CD 19 chimeric receptor T-cells, or T-cells expressing a tEGFR control vector were administered 7 days after tumor inoculation. All chimeric receptor constructs had the short IgG4 'Hinge-only' spacer and a 4- IBB costimulatory domain. (A, B) Serial bioluminescence imaging of tumor in cohorts of mice treated with T-cells expressing the 2A2 ROR1 chimeric receptor ( Y), the high affinity R12 ROR1 chimeric receptor (■), a CD19-specific chimeric receptor (A), with T-cells transduced with tEGFR alone (·), and untreated mice. Bioluminescence imaging showed tumor manifestations in the bone marrow and thorax and thus, signal intensity was measured in regions of interest that encompassed the entire body and thorax of each individual mouse. (C) Kaplan-Meier analysis of survival in individual treatment and control groups. Statistical analyses were performed using the log-rank test. The data shown in A-C are representative of results obtained in 2 independent experiments. (D) Proliferation of 2A2 ROR1 , R12 ROR1 and CD19 chimeric receptor T-cells in vivo. Tumor bearing NSG/JeKo-1 mice received a sing le dose of 5xl0 ⁶ CFSE- labeled 2A2 ROR1 , R12 ROR1 or CD19 chimeric receptor T-cells on day 7 after tumor inoculation, and 72 h later peripheral blood, bone marrow and spleen were collected from each individual mouse. The.frequency and proliferation of live (ΡΓ), CD45 ⁺ CD8 ⁺ tEGFR ^4" T-cells was analyzed. The frequency of 2A2 ROR1, R12 ROR1 and CD19 chimeric receptor T-cells respectively is provided on the left of each histogram as percentage of live cells, and the fraction of T-cells that underwent >4/3/2/l cell divisions is provided above each plot.

Figure 10 Expression of ROR1 and NKG2D ligands on epithelial cancer cell lines. (A) Expression of ROR1 on the triple negative breast cancer cell lines MDA-MB-231 and 468, and the renal cell cancer J ines FARP, TREP and RWL

(black histograms). Staining with matched isotype control antibody is shown as grey histograms. (B) Expression of CD80/86 and the NKG2D ligands MICA/B on MDA- MB-231 and Raji/RORl tumor ceils, and NKG2D (CD3 14) on 2A2 and R12 ROR1- chimeric receptor T-cells. Staining with matched isotype control mAbs is shown as grey dot plots histograms.

Figure 11: RORl-chimeric receptor modified T-cells recognize RORl ⁺ epithelial tumor cells in vitro. (A) Chromium release assay to evaluate the cytolytic activity of R12 ROR1 -chimeric receptor modified T-cells (short spacer/4- 1BB costimulatory domain, closed symbols) and tEGFR control T-cells (open symbols) against RORl ⁺ breast cancer and rena! cell cancer lines. (A-D) The 2A2 and R12 RORl-chimeric receptors had the optimal short spacer and a 4-1BB costimulatory domain. (B) Multiplex cytokine analysis after stimulation of T-cells expressing the 2 A2 and Rl 2 ROR1 -chimeric receptor with MDA-MB-231 and

Raji/RORl tumor cells. (C) Proliferation of CD8 ⁺ T-cells modified with the 2A2 and R12 RORl-chimeric receptor 72 hours after stimulation with MDA-MB-231 tumor cells. For analysis, triplicate wells were pooled and the proliferation of live (ΡΓ), CD8 ⁺ T-cells analyzed. Numbers above each histogram indicate the number of cell divisions the proliferating subset underwent, and the fraction of T-cells in each gate that underwent >4/3/2/l cell divisions is provided next to each histogram. (D) ELISA for IL-2 production by R12 RORl-chimeric receptor T-cells after a 24-hour co-culture with MDA-MB-231 in plain medium, and after addition of an antibody cocktail blocking of the NKG2D pathway [anti-NKG2D (clone 1D11), anti-MICA/B (clone 6D4) and anti-ULBP] or matched isotype control mAbs. O.D. of 0.6 corresponds to approximately 1900 pg ml.

Figure 12. Effect of extracellular spacer length on recognition and triggering of tumor cell lysis by CD8+ human T cells that express a HER2- specific chimeric receptor. A.) Depiction of Herceptin Fab epitope location on tumor cell membrane proximal epitope on human HER2, 13.) Structural formats of Herceptin scFv CAR spacer length variants as— T2A- linked polypeptides with the carboxyl EGFRt marker transmembrane protein, C.) Western biot detection of short, medium, and long spacer Herceptin-CAR variant expression in human CD8+ CTL's, D.) Flow cytometric detection of EGFRt by transduced human CD8+ CTL's transduced with Herceptin CAR variants then immunomagneticaliy purified by Herceptin-biotin, anti-biotin microbeads, E.) Distinct cytolytic function by T cells transduced to express the Herceptin CAR variants (short - S; medium - M; and long - L) against HER2 ⁺ Med41 1 FH and D283 human medulloblastoma cell lines (D341 is a HER2 ^" control medulloblastoma cell line, inset flow plots are tumor target lines stained with anti-HER2 specific mAb). Green=full IgG4 (Long Spacer, T),

Blue=IgG4hinge:CH3(Medium Spacer; A), Red=IgG4hinge only (Short Spacer;*).

Figure 13: CD19-chimeric receptor vectors and generation of CD19- chimeric receptor T cells.

(A) Design of lentiviral transgene inserts encoding a panel of CD19-specific chimeric receptors that differ in extracellular spacer length and intracellular co- stimulation. Each chimeric receptor encoded the CD19-specific single chain variable fragment derived from the FMC63 mAb in a VL- VH orientation, an IgG4-derived spacer domain of Hinge-CH2-CH3 (long spacer, 229 AA) or Hinge only (short spacer, 12 AA), and a signaling module containing CD3ζ with CD28 or 4- IBB alone or in tandem. Each chimeric receptor cassette contains a truncated EGFR marker encoded downstream of a cleavable 2A element, (B, C) Polyclonal T cell lines modified with each of the CD19-chimeric receptor constructs were prepared from purified CD8 ⁺ CD45RO ⁺ CD62L ⁺ central memory T cells (TCM) of normal donors. Following lentiviral transduction, transgene-positive T cells in each cell line were purified using the tEGFR marker and expanded for in vitro and in vivo experiments. (D) MFI after staining for the tEGFR marker shows equivalent transgene expression in T cells modified with each of the CD19-chimeric receptors.

Figure 14: In vitro cytotoxicity, cytokine production, and proliferation of T cells modified with distinct CD19-chimeric receptors. (A) Cytolytic activity of T cells expressing the various CD19-chimeric receptors against CD19 ⁺ and control target cells. (B) Multiplex cytokine assay of supernatants obtained after 24 hours from triplicate co-cultures of T cells expressing the various CD19-chimeric receptors and K562 cells transfected with CD 19, and CD19 ⁺ Raji cells. (C) Comparison of cytokine production by T cells expressing the various CD19- chimeric receptors. Multiplex cytokine data from 6 independent experiments were normalized (cytokine release by CD19-chimeric receptor ^: short/CD28 ^! CTL = 1) and analyzed by Student's t-test. (D) CFSE dye dilution was used to measure proliferation of CD19-chimeric receptor T cells 72 hours after stimulation with K562/CD19 (upper panel) and CD19 ⁺ Raj i tumor cells (lower panel) without addition of exogenous cytokines. For analysis, triplicate wells were pooled and the proliferation of live (ΡΓ), CD8 ⁺ T cells analyzed. Numbers above each histogram indicate the number of cell divisions the proliferating subset underwent, and the fraction of T cells in each gate that underwent >4/3/2/l cell divisions is provided in the upper left of each plot. (E) PI staining was performed at the end of a 72-hour co- culture of T cells expressing the various CD19-chhneric receptors with Raji tumor cells. The percentage of ΡΓ ^" cells within in chimeric receptor T cell line (CD3 ⁺ ) is provided in each histogram.

Figure 15: CD19-chimeric receptor T cells with a short extracellular spacer domain eradicate Raji tumors in NOD/SCID mice. (A) Cohorts of mice were inoculated with Raji-ffiuc via tail vein injection, and T cells transduced with CD19-chimeric receptors containing long and short spacer domains or with tEGFR alone were administered 2 and 9 days after tumor inoculation by tail vein injection. Tumor progression and distribution was evaluated by serial bioluminescence imaging after injection of luciferin substrate. (B) Serial bioluminescence imaging of tumor in cohorts of mice either treated with T cells expressing CD19-chimeric receptors with short spacer ('short/CD28' and 'short/4-lBB') and long spacer

('long/CD28' and 'long/4-lBB') domains, with T cells transduced with the tEGFR control vector, or untreated. Each diagram representing cohorts of mice treated with CD19-chimeric receptor or tEGFR transduced T cells also shows the mean of tumor progression in untreated mice for comparison (red triangles). (C) Kaplan-Meier analyses of survival of untreated mice and mice treated with T cells expressing

CD19-chimeric receptors with short spacer ('short/CD28' and 'short/4- IBB'), long spacer ( ^: long/CD28' and 'long/4- I BB') domains, and with control tEGFR.

Statistical analyses were performed using the log-rank test. The data shown in B and C are representative of results obtained in 3 independent experiments.

Figure 16: CD19-chimeric receptor T cells with a short spacer (short/4-

1BB) eradicate established Raji tumors in NSG mice in a dose-dependent manner. (A) Mice were inoculated with Raji-ffiuc via tail vein injection and tumor engraftment confirmed by bioluminescence imaging on day 6. On day 7, mice received a single i.v. injection of various doses of T cells transduced with the CD19- chimeric receptor 'short/4-lBB' or with the tEGFR-control lentivirus. (B, C) Dose dependent anti-tumor efficacy of T cells expressing the CDI 9-chimeric receptor 'short/4- I BB'. A control cohort of mice received a single high dose of T cells modified with tEGFR alone. (D) Persistence of CD 19-chimeric receptor T cells following adoptive transfer into NSG/Raji mice. Flow cytometric analysis of peripheral blood (eye bleeds) in the cohort of mice treated with 2.5x10 ⁶ CD19- chimeric receptor 'short/4-lBB' T cells. The frequency of CD8 ⁺ tEGFR ⁺ T cells is shown as percentage of live peripheral blood cells.

Figure 17: T cells expressing CD19-chimeric receptors with a short spacer and either CD28 or 4-1BB are more effective against established lymphoma than those expressing CD19-chimeric receptors with a long spacer. (A) NSG mice were inoculated with Raji-ffluc on day 0, and treated on day 7 with one dose of 2.5x10 ⁶ CD19 chimeric receptor T cells expressing short or long spacer and either CD28 or 4-1BB costimulatory domain. (B) Kaplan-Meier analyses of survival of mice in each of the treatment groups. Statistical analyses were performed using the log-rank test. (C) Bioluminescence imaging of cohorts of mice treated with T cells expressing CD19-chimeric receptors with short spacers ('short/CD28' and 'short/4- IBB'), and long spacers ('long/CD28 and 'long/4-lBB'). The mean tumor burden observed in untreated mice at each time point is shown in each diagram for comparison (triangles). (D) In vivo persistence of T cells expressing CD19-chimeric receptor with short spacer domain is enhanced compared to T cells expressing CD19-chimeric receptors with long spacer domain. The frequency of CD8 ⁺ tEGFR ⁺ T cells in the peripheral blood obtained at day 3 and 10 after transfer was determined by flow cytometry and is shown as percentage of live (ΡΓ) peripheral blood cells. Statistical analyses were performed by Student's t-test. The data shown in B-D are representative for results obtained in 3 independent experiments.

Figure 18: Increasing chimeric receptor T cell dose or augmenting costimulatory signaling does not improve the anti-tumor efficacy of CD19- chimeric receptors with a long spacer domain against established lymphoma. (A) Cytolytic activity of T cells expressing 'long/CD28', 'long/4-ΙΒΒ' and 'long/CD28 4- IBB' CD 19 chimeric receptors against CD19 ⁺ and control target cells. (B) Multiplex cytokine assay of supernatant obtained after 24 hours from triplicate co-cultures of K562/CD19 and Raji tumor cells with T cells expressing the various CD1 -chimeric receptors. (C) Evaluation of proliferation of CD19-chimeric receptor T cells 72 hours after stimulation with CD19 ⁺ tumor cells ( 562/CD19 - left panel; Raji— right panel) by CFSE dye dilution. For analysis, triplicate wells were pooled and the proliferation of live (ΡΓ) CD8 ⁺ T cells analyzed. Numbers above each histogram indicate the number of cell divisions the proliferating subset underwent, and the fraction of T cells in each gate that underwent >4/3/2/l cell divisions is provided in the upper left of each plot..(D) Kaplan-Meier analyses of survival of mice treated with T cells expressing CD 19-chimeric receptors with short ('short/CD28') and long spacer domain ('long/CD28' and 'long/CD28_4-lBB'), or T cells modified with a tEGFR-encoding control lentiviral vector. Statistical analyses were performed using the log-rank test. (E) Bioluminescence imaging of cohorts of mice treated with T cells expressing CD19-chimeric receptors with short spacer Cshort/CD28' and long spacers ('long/CD28 and 'long/CD28_4-lBB')- Diagrams show mean tumor progression in untreated mice for comparison (red triangles). (F) In vivo persistence of T cells expressing the various CD19-chimeric receptors. The frequency of CD8 ⁺ fEGFR ⁺ T cells in the peripheral blood obtained at day 3 and 10 after transfer was determined by flow cytometry and is shown as percentage of live (PT) peripheral blood cells. Statistical analyses were performed by Student's t-test.

Figure 19: CD 19-chimeric receptor T cells with a long spacer domain are activated by tumor in vivo but fail to increase in cell number. (A)

Expression of CD69 and CD25 on T cells modified with each CD19-chimeric receptor prior to transfer into NSG/Raji mice. (B) Cohorts of mice were inoculated with Raji-ffluc tumor cells and 7 days later received CFSE-labeled CD19-chimeric receptor transduced or control T cells. Bone marrow and spleens were harvested from subgroups of mice 24 and 72 hours after T cell administration. (C, D)

Multiparameter flow cytometric analysis of bone marrow mononuclear cells obtained 24 hours (C) and 72 hours (D) after T cell transfer. Dot plots show anti CD3 and anti CD45 staining after gating on ΡΓ cells to detect viable human T cells. The CD3 ^" CD45 gate contains Raj i tumor cells. Expression of CD25 and CD69 on live (ΡΓ) CD3 ⁺ CD45 ⁺ T cells is shown in the histograms. (E) Frequency of CD3 ⁺ CD45 ⁺ T cells in spleens obtained 24 and 72 hours after T cell transfer. Dot plots are gated on live ΡΓ splenocytes and the percentage of CD3 ¹ CD45 ¹ T cells is shown in each plot. (F) PI staining of bone marrow and splenocytes hours after T cell transfer into NSG Raji mice. The numbers in the histograms indicate the percentage of Pl ⁺ cells within the CD3 ⁺ population. (G) Bioluminescence imaging of cohorts of mice treated with T cells expressing CD19-chimeric receptors with short spacer ('short/CD28' and 'short 4- IBB'), long spacers ('!ong CD28 and 'long/4-lBB'), or control T cells.

Figure 20: T cells expressing CD19 chimeric receptors with 4-lBB and CD3zeta and a modified IgG4-Fc hinge exhibit superior in vitro and in vivo function compared to T cells expressing CD19 chimeric receptors with 4-lBB and CD3zeta and a CDS alpha hinge.A. Cytolytic activity of CD19 chimeric receptor modified T-cells with IgG4 Fc hinge, CDS alpha hinge and control T cells against Cr ⁵¹ -labeled K562 cells transfected with CD 19, Raji lymphoma cells that express CD 19, and K562 control T cells. Lysis is shown at different E/T ratios in a 4 hour Cr ⁵¹ release assay. B. Interferon gamma production by 5xl0 ⁴ T cells expressing a CD 19 chimeric receptor with an lgG4 Fc hinge or CD8 alpha hinge after a24-hour coculture with Raji tumor cells. O.D. of 1 corresponds to ~500 pg/ml of interferon gamma. C. CFSE dye dilution assay to measure proliferation of T cells expressing a CD 19 chimeric receptor with an IgG4 Fc hinge or CDS alpha hinge and T cells that express tEGFR alone (control) after 72 hours coculture with CD19 positive Raji lymphoma cells. Numbers above each histogram indicate the number of cell divisions the proliferating cell subset underwent. The fraction of T cells in each gate that underwent >3/2/l cell divisions is provided next to the plot. D. In vivo antitumor activity of T cells expressing a CD 19 chimeric receptor with an IgG4 Fc hinge (group 1) or CD8 alpha hinge {group 2) and T cells that express tEGFR alone (group 3) in NSG mice inoculated with Raji tumor cells expressing firefly luciferase (ffluc). Mice were imaged 17 days after tumor inoculation and 10 days after T cell inoculation. The data shows greater tumor burden in mice treated with control tEGFR T cells (group 3) or with CD 19 chimeric receptor CDS alpha hinge T cells (group 2) compared with mice treated with CD 19 chimeric receptor IgG4 Fc hinge T cells (group 1).

Detailed Description

Unless defined otherwise, ail technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which the invention pertains.

"About" as used herein when referring to a measurable value is meant to encompass variations of ±20% or ±10%, more preferably ±5%, even more preferably ±1%, and still more preferably ±0.1 % from the specified value,

"Activation", as used herein, refers to the state of a T cell that has been sufficiently stimulated to induce detectable cellular proliferation, cytokine production or expression of cell surface markers such as CD69 and CD25, or detectable effector functions.

"Activation Induced cell death" as used herein refers to a state of a T cell that is activated but is not able to proliferate for more than 2 generations and exhibits markers of apoptosis.

"Antigen" or "Ag" as used herein refers to a molecule that provokes an immune response. This immune response may involve either antibody production, or the activation of specific immunologically-competent cells, or both. Tt is readily apparent that an antigen can be generated synthesized, produced recombinantly or can be derived from a biological sample. Such a biological sample can include, but is not limited to a tissue sample, a tumor sample, a cell or a biological fluid.

"Anti-tumor effect" as used herein, refers to a biological effect, which can be manifested by a decrease in tumor volume, a decrease in the number of tumor cells, a decrease in the number of metastases, an increase in life expectancy, or a decrease of various physiological symptoms associated with the cancerous condition. An

"anti-tumor effect" can also be manifested by a decrease in recurrence or an increase in the time before recurrence.

"Chimeric receptor" as used herein refers to a synthetically designed receptor comprising a ligand binding domain of an antibody or other protein sequence that binds to a molecule associated with the disease or disorder and is linked via a spacer domain to one ore more intracellular signaling domains of a T cell or other receptors, such as a costimulatory domain.

"Co-stimulatory domain," as the term is used herein refers to a signaling moiety that provides to T cells a signal which, in addition to the primary signal provided by for instance the CD3 zeta chain of the TCR/CD3 complex, mediates a T cell response, including, but not limited to, activation, proliferation, differentiation, cytokine secretion, and the like. A co-stimulatory domain can include all or a portion of, but is not limited to, CD27, CD28, 4- I BB, OX40, CD30, CD40, , 1COS, lymphocyte function-associated antigen-1 LFA-1), CD2, CD7, LIGHT, NKG2C, B7-H3, and a ligand that specifically binds with CD83. In embodiments, the co- stimulatory domain is an intracellular signaling domain that interacts with other intracellular mediators to mediate a cell response including activation, proliferation, differentiation and cytokine secretion, and the like. "Coding for" are used herein refers to the property of specific sequences of nucleotides in a polynucleotide, such as a gene, a cDNA, or an mRNA, to serve as templates for synthesis of other macromolecules such as a defined sequence of amino acids. Thus, a gene codes for a protein if transcription and translation of mRNA corresponding to that gene produces the protein in a cell or other biological system. A "nucleic acid sequence coding for a polypeptide" includes all nucleotide sequences that are degenerate versions of each other and that code for the same amino acid sequence.

"Cytotoxic T lymphocyte "(CTL) as used herein refers to a T lymphocyte that expresses CDS on the surface thereof (i.e., a CD8 ⁺ T cell). In some

embodiments such cells are preferably "memory" T cells (TM cells) that are antigen- experienced.

"Central memory" T cell (or "TCM") as used herein refers to an antigen experienced CTL that expresses CD62L or CCR-7 and CD45RO on the surface thereof, and does not express or has decreased expression of CD45RA as compared to naive cells. In embodiments, central memory cells are positive for expression of CD62L, CCR7, CD28, CD127, CD45RO, and CD95, and have decreased expression of CD54RA as compared to na ^' ive cells.

"Effector memory" T cell (or "TE ") as used herein refers to an antigen experienced T cell that does not express or has decreased expression of CD62L on the surface thereof as compared to central memory cells, and does not express or has decreased expression of CD45RA as compared to na ^' ive cell. In embodiments, effector memory cells are negative for expression of CD62L andCCR7, compared to na'ive cells or central memory cells, and have variable expression of CD28 and CD45RA.

"Na ^' ive " T cells as used herein refers to a non antigen experienced T lymphocyte that expresses CD62L and CD45RA, and does not express CD45RO- as compared to central or effector memory cells. In some embodiments, na ^' ive CD8+ T lymphocytes are characterized by the expression of phenotypic markers of na ^' ive T cells including CD62L, CCR7, CD28, CD127, and CD45RA.

"Effector " "T _E " T cells as used herein refers to a antigen experienced cytotoxic T lymphocyte cells that do not express or have decreased expression of CD62L, CCR7, CD28, and are positive for granzyme B and perforin as compared to central memory or naive T cells.

"Enriched" and "depleted" as used herein to describe amounts of cell types in a mixture refers to the subjecting of the mixture of the cells to a process or step which results in an increase in the number of the "enriched" type and a decrease in the number of the "depleted" cells. Thus, depending upon the source of the original population of cells subjected to the enriching process, a mixture or composition may contain about 60, 70, 80, 90, 95, or 99 percent or more (in number or count) of the "enriched" cells and about 40, 30, 20, 10, 5 or 1 percent or less (in number or count) of the "depleted" cells.

"Epitope" as used herein refers to a part of an antigen or molecule that is recognized by the immune system including antibodies, T cells, and/ or B cells. Epitopes usually have at least 7 amino acids and can be linear or conformational.

"Isolated," when used to describe the various polypeptides disclosed herein, means polypeptide or nucleic acid that has been identified and separated and/or recovered from a component of its natural environment. Preferably, the isolated polypeptide or nucleic acid is free of association with all components with which it is naturally associated. Contaminant components of its natural environment are materials that would typically interfere with diagnostic or therapeutic uses for the polypeptide or nucleic acid, and may include enzymes, hormones, and other proteinaceous or non-proteinaceous solutes.

"Intracellular signaling domain" as used herein refers to all or a portion of one or more domains of a molecule (here the chimeric receptor molecule) that provides for activation of a lymphocyte. Intracellular domains of such molecules mediate a signal by interacting with cellular mediators to result in proliferation, differentiation, activation and other effector functions. In embodiments, such molecules include all or portions of CD28, CD3, 4-1 BB, and combinations thereof.

"Ligand" as used herein refers to a substance that binds specifically to another substance to form a complex. Example of ligands include epitopes on antigens, molecules that bind to receptors, substrates, inhibitors, hormones, and activators. "Ligand binding domain" as used herein refers to substance or portion of a substance that binds to a ligand. Examples of ligand binding domains include antigen binding portions of antibodies, extracellular domains of receptors, and active sites of enzymes. "Operably linked" as used herein refers to functional linkage between a regulatory sequence and a heterologous nucleic acid sequence resulting in expression of the latter. For example, a first nucleic acid sequence is operably linked with a second nucleic acid sequence when the first nucleic acid sequence is placed in a functional relationship with the second nucleic acid sequence. For instance, a promoter is operably linked to a coding sequence if the promoter affects the transcription or expression of the coding sequence. Generally, operably linked DNA sequences are contiguous and, where necessary to join two protein coding regions, in the same reading frame.

"Percent (%) amino acid sequence identity" with respect to the chimeric receptor polypeptide sequences identified herein is defined as the percentage of amino acid residues in a candidate sequence that are identical with the amino acid residues in the reference sequence for each of the ligand binding domain, spacer, transmembrane domain, and/or the lymphocyte activating domain, after aligning the sequences and introducing gaps, if necessary, to achieve the maximum percent sequence identity, and not considering any conservative substitutions as part of the sequence identity. Alignment for purposes of determining percent amino acid sequence identity can be achieved in various ways that are within the skill in the art, for instance, using publicly available computer software such as BLAST, BLAST-2, ALIGN, ALIGN-2 or Megalign (DNA STAR) software. Those skilled in the art can deterraine appropriate parameters for measuring alignment, including any algoritlims needed to achieve maxima! alignment over the full-length of the sequences being compared. For example, % amino acid sequence identity values generated using the WU-BLAST-2 computer program [Altschul et al., Methods in Enzymology, 266:460-480 (1996)] uses several search parameters, most of which are set to the default values. Those that are not set to default values (i.e., the adjustable parameters) are set with the following values: overlap span=l, overlap

fraction=0.125, word threshold (T)=l 1 and scoring matrix=BLOSUM62. A % amino acid sequence identity value is determined by dividing (a) the number of matching identical amino acid residues between the each or all of the polypeptide amino acid sequence of the reference chimeric receptor sequence provided in Table 2 and the comparison amino acid sequence of interest as determined by WU- BLAST-2 by (b) the total number of amino acid residues of the polypeptide of interest.

"Chimeric receptor variant polynucleotide" or "chimeric receptor variant nucleic acid sequence' ¹ as used herein refers to a polypeptide-encoding nucleic acid molecule as defined below having at least about 80% nucleic acid sequence identity with the polynucleotide acid sequence shown in Table 1 or a specifically derived fragment thereof, such as polynucleotide coding for an antigen binding domain, a polynucleotide encoding a spacer domain, a polynucleotide coding for a

transmembrane domain and/ or a polynucleotide coding for a lymphocyte stimulatory domain. Ordinarily, a chimeric receptor variant of polynucleotide or fragment thereof will have at least about 80% nucleic acid sequence identity, more preferably at least about 81 % nucleic acid sequence identity, more preferably at least about 82% nucleic acid sequence identity, more preferably at least about 83% nucleic acid sequence identity, more preferably at least about 84% nucleic acid sequence identity, more preferably at least about 85% nucleic acid sequence identity, more preferably at least about 86% nucleic acid sequence identity, more preferably at least about 87% nucleic acid sequence identity, more preferably at least about 88% nucleic acid sequence identity, more preferably at least about 89% nucleic acid sequence identity, more preferably at least about 90% nucleic acid sequence identity, more preferably at least about 91% nucleic acid sequence identity, more preferably at least about 92% nucleic acid sequence identity, more preferably at least about 93% nucleic acid sequence identity, more preferably at least about 94% nucleic acid sequence identity, more preferably at least about 95% nucleic acid sequence identity, more preferably at least about 96% nucleic acid sequence identity, more preferably at least about 97% nucleic acid sequence identity, more preferably at least about 98% nucleic acid sequence identity and yet more preferably at least about 99% nucleic acid sequence identity with the nucleic acid sequence as shown in Table or a derived fragment thereof. Variants do not encompass the native nucleotide sequence. In this regard, due to the degeneracy of the genetic code, one of ordinary skill in the art will immediately recognize that a large number of chimeric receptor variant polynucleotides having at least about 80% nucleic acid sequence identity to the nucleotide sequence of Table 1 will encode a polypeptide having an amino acid sequence which is identical to the amino acid sequence of Table 2. "Substantially purified" refers to a molecule that is essentially free of other molecule types or a cell that is essentially free of other cell types. A substantially purified cell also refers to a cell, which has been separated from other cell types with which it is normally associated in its naturally occurring state. In some instances, a population of substantially purified cells refers to a homogenous population of cells.

"Not substantially found" when used in reference the presence of a tumor antigen or other molecules on normal cells refers to the percentage of a normal cell type that has the antigen or molecule, and / or the density of the antigen on the cells. In embodiments, not substantially found means that the antigen or molecule is found on less than 50% of normal cel l type and/or at a 50% less density as compared to the amount of cells or antigen found on a tumor ceil or other diseased cell.

"T cells" or "T lymphocytes" as used herein may be from any mammalian, preferably primate, species, including monkeys, dogs, and humans. In some embodiments the T cells are allogeneic (from the same species but different donor) as the recipient subject; in some embodiments the T cells are autologous (the donor and the recipient are the same); in some embodiments the T cells arc syngeneic (the donor and the recipients are different but are identical twins).

Modes of the Disclosure

The disclosure provides for chimeric receptor nucleic acids, and vectors and host cells including such nucleic acids. The chimeric receptor nucleic acid comprises a number of modular components that can be excised and replaced with other components in order to customize the chimeric receptor for a specific target molecule. The disclosure provides that one of the modular components is the spacer component. It has been surprisingly found that the length of the spacer region that is presumed not to have signaling capability affects the in vivo efficacy of the T cells modified to express the chimeric receptor and needs to be customized for individual target molecules for enhanced therapeutic activity.

In one aspect, methods and nucleic acid constructs are provided to design a chimeric receptor that has improved tumor recognition, increased T cell proliferation and/or cytokine production in response to the ligand as compared to a reference chimeric receptor. In embodiments, a library of nucleic acids is provided, wherein each nucleic acid codes for a spacer region that differs from the others in sequence and length. Each of the nucleic acids can then be used to form a chimeric receptor nucleic acid construct that can be tested in vivo (in an animal model) and/or in vitro so that a spacer can be selected that provides for improved tumor recognition, increased T cell proliferation and/or cytokine production in response to the ligand.

In embodiments, a chimeric receptor nucleic acid comprises a

polynucleotide coding for a ligand binding domain, wherein the ligand is a tumor or viral specific antigen or molecule, a polynucleotide coding for a customized polypeptide spacer, wherein the spacer provides for enhanced T cell proliferation; a polynucleotide coding for a transmembrane domain; and a polynucleotide coding for one or more intracellular signaling domains. In embodiments, a long spacer is employed if the epitope of the target molecule is membrane proximal on the target cell and a short spacer is employed if the epitope of the target molecule is membrane distal on the target cell.

The design of a chimeric receptor can be customized depending on the type of tumor or virus, the target antigen or molecule present on the tumor, the affinity of the antibody for the target molecule, the flexibility needed for the antigen binding domain, and/or the intracellular signaling domain. In

embodiments, a number of chimeric receptor constructs are tested in vitro and in in vivo models to determine the ability of T cells modified with the receptor to kill tumor cells in immunodeficient mice and to proliferate and persist after adoptive transfer. In embodiments, a chimeric receptor is selected that provides for capability of at least 30% of the cells to proliferate through at least two generations in vitro and/or within 72 hours after introduction in vivo. In

embodiments, a chimeric receptor is not selected that results in greater than 50% of the cel ls undergoing activation induced cell death (AICD) within 72 hours in vivo in immunodeficient mice, and fails to eradicate tumor cells.

Depending on whether the target molecule is present on a subject's tumor cells, the chimeric receptor i ncludes a ligand binding domain that specifically binds to that target molecule. In embodiments, a subject's tumor ceils are characterized for cell surface tumor molecules. The target molecule may be selected based on a determination of its presence on a particular subject's tumor cells. In embodiments, a target molecule is selected that is a cell surface molecule found predominantly on tumor cells and not found on normal tissues to any substantial degree. In embodiments, an antibody is selected to bind to an epitope on the targeted cell surface molecule. In some cases, the epitope is characterized with respect to its proximity to the cell membrane. An epitope is characterized as proximal to the membrane when it is predicted or known by structural analysis to reside closer to the target cell membrane than alternative epitopes that are predicted or known by structural analysis to reside a greater distance from the target cell membrane. In embodiments, the affinity of the antibody from which the scFV is constructed is compared by binding assays, and antibodies with different affinities are examined in chimeric receptor formats expressed in T cells to determine which affinity confers optimal tumor recognition, based on superior cytotoxicity of target cells, and/or T cell cytokine production and proliferation.

In addition, the spacer region of the chimeric receptor may be varied to optimize T cell recognition of the ligand on the target cell. In embodiments, when an antibody binds to an epitope on the target cell that is very proximal to the membrane, a spacer is selected that is longer than about 15 amino acids. For example, in embodiments, if the epitope or portion thereof on the target antigen is in the first 100 amino acids of the linear sequence of the extracellular domain adjacent to the transmembrane domain, a long spacer region may be selected. In

embodiments, when an antibody binds to an epitope on the target cell that is distal to the membrane, a spacer is selected that is about 1 19 or 1 5 amino acids or less. For example, in embodiments, when the epitope or portion thereof is found in the 150 amino acids of the linear sequence of the extracellular domain from the terminus, a short or inetermediate spacer may be utilized. In embodiments, a spacer comprises an ammo acid sequence XiPPX ₂ P.

A variety of combinations of primary and costimulatory intracellular signaling domain may be employed to enhance the in vivo efficacy of the chimeric receptor. In embodiments, different constructs of the chimeric receptor can be tested in an in vivo animal model to determine efficacy for tumor killing. In embodiments, a costimulatory intracellular signaling domain is selected from the group consisting of CD28 and modified versions thereof, 4- 1 BB and modified versions thereof and combinations thereof. Other costimulatory domains, such as OX40 may be incorporated. CD8+ central memory T cells have an intrinsic programming that allows them to persist for extended periods after administration, which makes them a preferred subset of CD8+ T cells for immunotherapy. In embodiments, CDl 9 specific chimeric receptor modified cytotoxic T cells prepared from sort purified CD8+ central memory T cells are administered in the presence or absence of CD4+ CD19 specific chimeric receptor -modified T cells. In embodiments, tumor-specific CD4+ T cells exert anti-tumor reactivity and provide help to tumor-specific CD8+ T cells in vitro and in vivo. In a specific embodiment, tumor-specific CD4+ T cells or CD4' T cells selected from the naive or the central memory subsets are utilized alone or in combination with CD8 ⁺ TCM-

Nucleic Acids, Vectors, and polypeptides

The disclosure provides a chimeric receptor nucleic acid useful for transforming or transducing lymphocytes for use in adoptive immunotherapy. In embodiments, the nucleic acid contains a number of modular components that provide for easy substitution of elements of the nucleic acid. While not meant to limit the scope of the disclosure, it is believed that the chimeric receptor for each tumor antigen is desirably customized in terms of components in order to provide for in vivo efficacy and efficient expression in mammalian cells. For example, in a specific embodiment, for efficacy of a chimeric receptor comprising a scFV that binds to a ROR1 epitope located in the membrane distal Ig/Frizzled domain, a spacer that is about 15 amino acids or less is employed. In another specific embodiment, for efficacy of a chimeric receptor comprising a scFV mat binds to a ROR1 epitope located in the membrane proximal Kringle domain, a spacer that is longer than 15 amino acids is employed. In another embodiment, for efficacy of a chimeric receptor comprising a scFV that binds to CDl 9, a spacer that is 15 amino acids or less is employed.

In embodiments, an isolated chimeric receptor nucleic acid comprises a polynucleotide coding for a ligand binding domain, wherein the target molecule is a tumor specific antigen, a polynucleotide coding for a polypeptide spacer wherein the polypeptide spacer is about 229 amino acids or less; a polynucleotide coding for a transmembrane domain; and a polynucleotide coding for an intracellular signaling domain. In embodiments, an expression vector comprises a chimeric nucleic acid as described herein. Polypeptides encoded by all of or a portion of the chimeric receptor nucleic acids are also included herein.

Ligand binding domain

In embodiments, the chimeric receptor nucleic acid comprises a

polynucleotide coding for a ligand binding domain. In embodiments, the ligand binding domain specifically binds to a tumor or viral specific antigen. In embodiments, the ligand binding domain is an antibody or fragment thereof. A nucleic acid sequence coding for an antibody or antibody fragment can readily be determined. In a specific embodiment, the polynucleotide codes for a single chain Fv that specifically binds CD19. In other specific embodiments, the polynucleotide codes for a single chain Fv that specifically binds OR1. The sequences of these antibodies are known to or can readily be determined by those of skill in the art.

Tumor antigens are proteins that are produced by tumor cells that elicit an immune response. The selection of the ligand binding domain of the invention will depend on the type of cancer to be treated, and may target tumor antigens or other tumor cell surface molecules. A tumor sample from a subject may be characterized for the presence of certain biomarkers or cell surface markers. For example, breast cancer cells from a subject may be positive or negative for each of Her2Neu, Estrogen receptor, and/or the Progesterone receptor. A tumor antigen or cell surface molecule is selected that is found on the individual subject's tumor cells. Tumor antigens and cell surface molecules are well known in the art and include, for example, carcinoembryonic antigen (CEA), prostate specific antigen, PSMA, Her2/neu, estrogen receptor, progesterone receptor, ephrinB2, CD 19, CD20, CD22, CD23, CD123, CS-1 , ROR1, mesothelin, c-Met, GD-2, and MAGE A3 TCR. In embodiments a target molecule is a cell surface molecule that is found on tumor cells and is not substantially found on nonnal tissues, or restricted in its expression to non-vital normal tissues.

Other target molecules include but are not limited to antigens derived from infectious pathogens such as HIV (human immunodeficiency virus), HBV (hepatitis B virus), HPV (human papilloma virus) and Hepatitis C virus.

In one embodiment, the target molecule on the tumor comprises one or more epitopes associated with a malignant tumor. Malignant tumors express a number of proteins that can serve as target antigens for T cell receptor or chimeric receptor mediated recognition. Other target molecules belong to the group of cell transformation-related molecules such as the oncogene HER-2/Neu ErbB2. In embodiments, the tumor antigen is selectively expressed or overexpressed on the tumor cells as compared to control cells of the same tissue type. In other embodiments, the tumor antigen is a cell surface polypeptide.

Once a tumor cell surface molecule that might be targeted with a chimeric receptor is identified, an epitope of the target molecule is selected and characterized. In embodiments, an epitope is selected that is proximal to the tumor cell membrane. In other embodiments, an epitope is selected that is distal to the tumor cell membrane. An epitope is characterized as proximal to the membrane when it is predicted or known by structural analysis to reside closer to the target cell membrane than alternative epitopes that are predicted or known by structural analysis to reside a greater distance from the target cell membrane.

Antibodies that specifically bind a tumor cell surface molecule can be prepared using methods of obtaining monoclonal antibodies, methods of phage display, methods to generate human or humanized antibodies, or methods using a transgenic animal or plant engineered to produce human antibodies. Phage display libraries of partially or fully synthetic antibodies are available and can be screened for an antibody or fragment thereof that can bind to the target molecule. Phage display libraries of human antibodies are also available. In embodiments, antibodies specifically bind to a tumor cell surface molecule and do not cross react with nonspecific components such as bovine serum albumin or other unrelated antigens. Once identified, the amino acid sequence or polynucleotide sequence coding for the antibody can be isolated and/or determined.

Antibodies or antigen binding fragments include all or a portion of polyclonal antibodies, a monoclonal antibody, a human antibody, a humanized antibody, a synthetic antibody, a chimeric antibody, a bispecific antibody, a minibody, and a linear antibody. Antibody fragments" comprise a portion of an intact antibody, preferably the antigen binding or variable region of the intact antibody and can readily be prepared. Examples of antibody fragments include Fab, Fab', F(ab')2, and Fv fragments; diabodies; linear antibodies; single-chain antibody molecules; and multispecific antibodies formed from antibody fragments. In embodiments, a number of different antibodies that bind to a particular tumor cell surface molecules can be isolated and characterized. In embodiments, the antibodies are characterized based on epitope specificity of the targeted molecule, In addition, in some cases, antibodies that bind to the same epitope can be selected based on the affinity of the antibody for that epitope. In embodiments, an antibody has an affinity of at least 1 mM, and preferably <50 nM. In embodiments, an antibody is selected that has a higher affinity for the epitope as compared to other antibodies. For example, an antibody is selected that has at least a 2 fold, at least a 5 fold, at least a 10 fold, at least a 20 fold, at least a 30 fold, at least a 40 fold, or at least a 50 fold greater affinity than a reference antibody that binds to the same epitope.

In embodiments, target molecules are selected from the group consisting of CD19, CD20, CD22, CD23, CD123, CS-l , ROR1 , mesothelin, Her2, c-Met, PSMA, GD-2, MAGE A3 TCR and combinations thereof.

In specific embodiments, the target antigen is CD19. A number of antibodies specific for CD 19 are known to those of skill in the art and can be readily characterized for sequence, epitope binding, and affinity. In a specific embodiment, the chimeric receptor construct includes a scFV sequence from FMC63 antibody. In other embodiments, the scFV is a human or humanized ScFv comprising a variable light chain comprising a CDRL1 sequence of RASQDIS YLN, CDRL2 sequence of SRLHSGV, and a CDRL3 sequence of GNTLPYTFG. In other embodiments, the scFV is a human or humanized ScFv comprising a variable heavy chain comprising CDRH1 sequence of DYGVS , CDRH2 sequence of VI WGSETTYYNS ALKS, and a CDRH3 sequence of YAMDYWG. The disclosure also contemplates variable regions that have at least 90% amino acid sequence identity to that of the scFv for FMC63 and that have at least the same affinity for CD 19. In embodiments, the chimeric receptor has a short or intermediate spacer of 1 19 amino acids or less, or 12 amino acids or less. In a specific embodiment, the spacer is 12 amino acid or less and has a sequence of SEQ ID NO:4,

In embodiments, CDR regions are found within antibody regions as numbered by Kabat as follows: for the light chain; CDR.L1 amino acids 24- 34;CDRL2 amino acids 50-56; CDRL3 at amino acids 89-97 ; for the heavy chain at CDRHl at amino acids 31-35; CDRH2 at amino acids 50-65; and for CDRH3 at amino acids 95-102. CDR regions in antibodies can be readily determined.

In specific embodiments, the target antigen is RORl . A number of antibodies specific for RORl are known to those of skill in the art and can be readily characterized for sequence, epitope binding, and affinity. In a specific embodiment, the chimeric receptor construct includes a scFV sequence from R12 antibody. In other embodiments, the scFV is a human or humanized ScFv comprising a variable light chain comprising a CDRL1 sequence of ASGFDFSAYYM, CDRL2 sequence of T1YPSSG, and a CDRL3 sequence of ADRATYFCA. In other embodiments, the scFV is a human or humanized ScFv comprising a variable heavy chain comprising CDRHl sequence of DTDDWY, CDRH2 sequence of VQSDGS YTKRPG VPDR, and a CDRH3 sequence of YIGGYVFG. The disclosure also contemplates variable regions that have at least 90% amino acid sequence identity to that of the scFv for R12 and that have at least the same affinity for RORl . In embodiments, the chimeric receptor has a short or intermediate spacer of 119 amino acids or less, or 12 amino acids or less. In a specific embodiment, the spacer is 12 amino acid or less and has a sequence of SEQ ID NO:4.

In specific embodiments, the target antigen is RORl . A number of antibodies specific for RORl are known to those of skill in the art and can be readily characterized for sequence, epitope binding, and affinity. In a specific embodiment, the chimeric receptor construct includes a scFV sequence from R l 1 antibody. In other embodiments, the scFV is a human or humanized ScFv comprising a variable light chain comprising a CDRL1 sequence of SGSD1NDYPIS, CDRL2 sequence of INSGGST, and a CDRL3 sequence of YFCARGYS. In other embodiments, the scFV is a human or humanized ScFv comprising a variable heavy chain comprising CDRHl sequence of SNLAW, CDRH2 sequence of RASNLASGVPSRFSGS, and a CDRH3 sequence of NVSYRTSF. The disclosure also contemplates variable regions that have at least 90% amino acid sequence identity to that of the scFv for Rl 1 and that have at least the same affinity for RORl . In embodiments, the chimeric receptor has a long spacer of 229 amino acids or less. In a specific embodiment, the spacer is 229 amino acids and has a sequence of SEQ ID NO:50.

In specific embodiments, the target antigen is Her2. A number of antibodies specific for Her2 are known to those of skill in the art and can be readily characterized for sequence, epitope binding, and affinity. In a specific embodiment, the chimeric receptor construct includes a scFV sequence from Herceptin antibody. In other embodiments, the scFV is a human or humanized ScFv comprising a variable liglit chain comprising a CDRLl sequence, CDRL2 sequence and a CDRL3 sequence of the Herceptin antibody. In other embodiments, the scFV is a human or humanized ScFv comprising a variable heavy chain comprising CDRH1 sequence, CDRH2, and a CDRH3 sequence of Herceptin. The CDR sequences can readily be determined from the amino acid sequence of Herceptin. The disclosure also contemplates variable regions that have at least 90% amino acid sequence identity to that of the scFv for Herceptin and that have at least the same affinity for Her2. In embodiments, the chimeric receptor has a long spacer of 229 amino acids or less. In a specific embodiment, the spacer is 229 amino acids and has a sequence of SEQ ID NO:50.

In embodiments, a polynucleotide coding for a ligand binding domain is operably linked to a polynucleotide coding for a spacer region. In embodiments, the polynucleotide coding for a ligand binding domain may also have one or more restriction enzyme sites at the 5 ' and/or 3' ends of the coding sequence in order to provide for easy excision and replacement of the polynucleotide with another polynucleotide coding for a ligand binding domain coding for a different antigen or that has different binding characteristics. For example, a restriction site, Nhel, is encoded upstream of the leader sequence; and a 3' Rsrll located within the hinge region allows subcloning of any desirable scFv into a chimeric receptor vector. In embodiments, the polynucleotide is codon optimized for expression in mammalian cells.

In embodiments, the polynucleotide coding for a ligand binding domain is operably linked to a signal peptide. In embodiments the signal peptide is a signal peptide for granulocyte colony stimulating factor. Polynucleotides coding for other signal peptides such as CD8 alpha can be utilized.

In embodiments, the polynucleotide coding for a !igand binding domain is operably linked to a promoter. A promoter is selected that provides for expression of the chimeric antigen receptor in a mammalian cell. In a specific embodiment the promoter is the elongation growth factor promoter (EF- 1 ). Another example of a suitable promoter is the immediate early cytomegalovirus (CMV) promoter sequence. However, other constitutive promoter sequences may also be used, including, but not limited to the simian virus 40 (SV 40) early promoter, mouse mammary tumor virus (MMTV), human immunodeficiency virus (HIV) long terminal repeat (LTR) promoter, MuMoLV promoter, an avian leukemia virus promoter, an Epstein-Barr virus immediate early promoter, a Rous sarcoma virus promoter, as well as human gene promoters such as, but not limited to, the actin promoter, the myosin promoter, the hemoglobin promoter, and the creatine kinase promoter. Inducible promoters are also contemplated. Examples of inducible promoters include, but are not limited to a metallothionine promoter, a

glucocorticoid promoter, a progesterone promoter, and a tetracycline promoter.

A specific embodiment of a polynucleotide coding for a ligand binding domain is shown in Table 1 as the scFv from an antibody that specifically binds CD19, such as FMC63. A polynucleotide encoding for a flexible linker including the amino acids GSTSGSGKPGSGEGSTKG (SEQ ID NO:36)separates the VH and VL chains in the scFV. The amino acid sequence of the scFv including the linker is shown in Table 2.(SEQ ID NO: l 1) Other CD19-targeting antibodies such as SJ25C1 and HD37 are known. (SJ25C1 : Bejcek et al. Cancer Res 2005, PMID 7538901 ; HD37: Pezutto et al. JI 1987, PMID 2437199).

Spacer

In embodiments, the chimeric receptor nucleic acid comprises a

polynucleotide coding for a spacer region. It has been surprisingly found that the length of the spacer region that is presumed not to have signaling capability affects the in vivo efficacy of the T cells modified to express the chimeric receptor and needs to be customized for individual target molecules for optimal tumor or target cell recognition. In embodiments, the chimeric receptor nucleic acid comprises a polynucleotide coding for a customizable spacer region selected from a library of polynucleotides coding for spacer regions. In embodiments, a spacer length is selected based upon the location of the epitope, affinity of the antibody for the epitope, and/or the ability of the T cells expressing the chimeric receptor to proliferate in vitro and/or in vivo in response to antigen recognition.

Typically a spacer region is found between the ligand binding domain and the transmembrane domain of the chimeric receptor. In embodiments, a spacer region provides for flexibility of the ligand binding domain, allows for high expression levels in lymphocytes. A CD19-specific chimeric receptor having a spacer domain of about 229 amino acids had less antitumor activity than a CD 19- specific chimeric receptor with a short spacer region comprised of the modified IgG4 hinge only. Other chimeric receptors, such as those constructed from the R12 or 2A2 scFvs also require a short spacer for optimal triggering of T cell effector functions, while a chimeric receptor constructed with the Rl l ROR1 scFv requires a long spacer domain of about 229 amino acids for tumor recognition.

In embodiments, a spacer region has at least about 10 to 229 amino acids, about 10 to 200 amino acids, about 1 0 to 175 amino acids, about 10 to 150 amino acids, about 10 to 125 amino acids, about 10 to 100 amino acids, about 10 to 75 amino acids, about 10 to 50 amino acids, about 10 to 40 amino acids, about 10 to 30 amino acids, about 10 to 20 amino acids, or about 10 to 15 amino acids, and including any integer between the endpoints of any of the listed ranges. In embodiments, a spacer region has about 12 amino acids or less, about 1 19 amino acids or less, or about 229 amino acids or less.

In some embodiments, the spacer region is derived from a hinge region of an immunoglobulin like molecule. In embodiments, a spacer region comprises all or a portion of the hinge region from a human IgG 1 , human IgG2, a human IgG3, or a human IgG4, and may contain one or more amino acid substitutions. Exemplary sequences of the hinge regions are provided in Table 8. In embodiments, a portion of the hinge region includes the upper hinge amino acids found between the variable heavy chain and the core, and the core hinge amino acids including a poly pro line region. Typically, the upper hinge region has about 3 to 10 amino acids. In some cases, the spacer region comprises an amino acid sequence of XjPPX2P(SEQ ID NO: l). In embodiments, X] is a cysteine, glycine, or arginine and X2 is a cysteine or a threonine.

In embodiments, hinge region sequences can be modified in one or more amino acids in order to avoid undesirable structural interactions such as

dimerization. In a specific embodiment, the spacer region comprises a portion of a modified human hinge region from IgG4, for example, as shown in Table 2 or Table 8(SEQ ID NO:21 ). A representative of a polynucleotide coding for a portion of a modified IgG4 hinge region is provided in Table 1. (SEQ ID NO:4)In embodiments, a hinge region can have at least about 90%, 92%, 95%, or 100% sequence identity with a hinge region amino acid sequence identified in Table 2 or Table 8. In a specific embodiment, a portion of a human hinge region from IgG4 has an amino acid substitution in the core amino acids from CPSP to CPPC.

In some embodiments, all or a portion of the hinge region is combined with one or more domains of a constant region of an immunoglobulin. For example, a portion of a hinge region can be combined with all or a portion of a CH2 or CH3 domain or variant thereof. In embodiments, the spacer region does not include the 47-48 amino acid hinge region sequence from CD8apha or the spacer region consisting of an extracellular portion of the CD28 molecule.

In embodiments, a short spacer region has about 12 amino acids or less and comprises all or a portion of a IgG4 hinge region sequence or variant thereof, an intermediate spacer region has about 1 19 amino acids or less and comprises all or a portion of a TgG4 hinge region sequence and a CH3 region or variant thereof, and a long spacer has about 229 amino acids or less and comprises all or a portion of a IgG4 hinge region sequence , a CH2 region, and a CH3 region or variant thereof.

A polynucleotide coding for a spacer region can be readily prepared by synthetic or recombinant methods from the amino acid sequence. In embodiments, a polynucleotide coding for a spacer region is operab!y linked to a polynucleotide coding for a transmembrane region. In embodiments, the polynucleotide coding for the spacer region may also have one or more restriction enzyme sites at the 5' and/or 3' ends of the coding sequence in order to provide for easy excision and replacement of the polynucleotide with another polynucleotide coding for a different spacer region. In embodiments, the polynucleotide coding for the spacer region is codon optimized for expression in mammalian cells.

In embodiments, a library of polynucleotides, each coding for different spacer region is provided. In an embodiment, the spacer region is selected from the group consisting of a hinge region sequence from IgGl, IgG2, lgG3, or IgG4 or portion thereof, a hinge region sequence from IgGl , IgG2, TgG3, or IgG4 in combination with all or a portion of a CH2 region or variant thereof, a hinge region sequence from IgGl , lgG2, IgG3, or IgG4 in combination with all or a portion of a CH3 region or variant thereof, and a hinge region sequence from IgGl , TgG2, IgG3, or !gG4 in combination with all or a portion of a CH2 region or variant thereof, and a CH3 region or variant thereof. In embodiments, a short spacer region is a modified lgG4 hinge sequence(SEQ ID NO:4) having 12 amino acids or less, an intermediate sequence is a IgG4 hinge sequence with a CH3 sequence having 119 amino acids or less(SEQ ID NO:49); or a IgG4 hinge sequence with a CH2 and CH3 region having 229 amino acids or less (SEQ ID NO:50)

In embodiments, a method of selecting a spacer region for a chimeric receptor is provided herein. Surprisingly some chimeric receptor constructs, although effective to activate T cells and direct their killing of tumor cells in vitro, were not effective in vivo. In addition, the side effect profile of the chimeric receptor modified T cells can be such as to result in more cells undergoing activation induced cell death or causing an increase in in vivo cytokines. In embodiments, a method comprises providing a plurality of chimeric receptor nucleic acids, wherein the chimeric receptor nucleic acids differ only in the spacer region; introducing each of the chimeric receptor nucleic acids into a separate T lymphocyte population;

expanding each separate lymphocyte population in vitro, and introducing each l mphocyte population into an animal bearing a tumor to determine the anti-tumor efficacy of each of the chimeric receptors when expressed in T cells, and selecting a chimeric receptor that provides anti-tumor efficacy as compared to each of the other separate lymphocyte populations modified with each of the other chimeric receptors.

Animal models of different tumors are known. Anti-tumor efficacy can be measured by identifying a decrease in tumor volume, by determining animal death, persistence of the genetically modified T cells in vivo, activation of genetically modified T cells (for example, by detecting an increase in expression of CD25 and/CD69), and/or proliferation of genetically modified T cells in vivo. In an embodiment, a chimeric receptor is selected that provides for the best anti-tumor efficacy in vivo as determined by one or more of these parameters. Lack of antitumor efficacy can be determined by lack of persistence of the genetically modified lymphocytes in vivo, animal death, an increase in apoptosis as measured by an increase in induction of caspase -3, and/or a decrease in proliferation of genetically modified lymphocytes.

In other embodiments, a method for selecting a spacer comprises selecting an epitope of a target molecule and characterizing the location of the epitope with respect to the cell membrane, selecting a spacer region that is long or short depending on the location of the epitope with respect to the cell membrane, selecting an antibody or fragment thereof that has an affinity for the e itope that is higher or lower as compared to a reference antibody, and determining whether the chimeric receptor construct provides for enhanced T cell proliferation or cytokine production in vitro and/or in vivo.

Tn some embodiments, if the target epitope or portion thereof is located proximal to the membrane it is located in the first 100 amino acids of the linear sequence of the extracellular domain adjacent to the transmembrane domain. If the epitope is located proximal to the membrane, a long spacer(e.g .229 amino acids or less and greater than 1 19 amino acids) is selected. In some embodiments, if the target epitope is located distal to the membrane, it is located in the first 150 amino acids of the linear sequence of the extracellular domain terminus. If the epitope is located distal to the membrane, an intermediate or short spacer is selected (e.g. 1 19 amino acids or less or 12-15 amino acids or less). Alternatively, whether the epitope is proximal or distal to the membrane can be determined by modeling of the three dimensional structure or based on analysis of the crystal structure,

In some embodiments, a chimeric receptor is selected that provides for at least 30% of the cells proliferating through two generations in vitro and/or in vivo. In other embodiments a chimeric receptor is not selected if it results in at least 50% of the cells undergoing activation induced cell death in 72 hours. In embodiments, a short spacer (e.g. 1 5 amino acids or less) is selected if the epitope is distal to the membrane. In embodiments, a long spacer (e.g. 229 amino acid or less and greater than j 19 amino acids) is selected if the epitope is proximal to the membrane.

In embodiments, providing a plurality of chimeric receptor nucleic acids, wherein the chimeric receptor nucleic acids differ only in the spacer region comprises providing a chimeric receptor construct comprising a polynucleotide coding for a ligand binding domain, wherein the ligand is a tumor specific antigen, viral antigen, or any other molecule expressed on a target cell population that is suitable to mediate recognition and elimination by a lymphocyte; a polynucleotide coding for a first polypeptide spacer having a defined restriction site at the 5 ' and 3 ' end of the coding sequence for the first polypeptide spacer; a polynucleotide coding for a transmembrane domain; and a polynucleotide coding for one or more intracellular signaling domains. In embodiments, a method further comprises providing one or more polynucleotides, each encoding a different spacer region. In embodiments, the different spacer regions are selected from the group consisting of a hinge region sequence from IgGl , IgG2, IgG3, or IgG4 or variant thereof or portion thereof, a hinge region sequence from IgGl, IgG2, IgG3, or IgG4 in combination with all or a portion of a CH2 region or variant thereof, a hinge region sequence from IgGl, IgG2, IgG3, or IgG4 in combination with all or a portion of a CH3 region or variant thereof, and a hinge region sequence from IgGl, IgG2, IgG3, or IgG4 in combination with all or a portion of a CH2 region or variant thereof and a CH3 region or variant thereof. In embodiments, CH2 or CH3 regions may be modified by one or more deletions or amino acid substitutions in order to provide for expression in lymphocytes and/or in order to minimize interactions with other molecules. In embodiments, a portion of a hinge region comprises at least the upper amino acids and the core sequence. In embodiments, a hinge region comprises the sequence X]PPX ₂ P.

In embodiments, a method further comprises replacing the polynucleotide coding for the spacer region with a polynucleotide encoding a different spacer region to form a chimeric receptor nucleic acid with a different spacer region. The method can be repeated to form any number of chimeric receptor nucleic acids, each differing in the spacer region. In embodiments, the chimeric receptor nucleic acids differ from one another only in the spacer region.

Transmembrane domain

In embodiments, the chimeric receptor nucleic acid comprises a

polynucleotide coding for a transmembrane domain. The transmembrane domain provides for anchoring of the chimeric receptor in the membrane.

In an embodiment, the transmembrane domain that naturally is associated with one of the domains in the chimeric receptor is used. In some cases, the transmembrane domain can be selected or modified by amino acid substitution to avoid binding of such domains to the transmembrane domains of the same or different surface membrane proteins to minimize interactions with other members of the receptor complex.

The transmembrane domain may be derived either from a natural or a synthetic source. When the source is natural, the domain may be derived from any membrane-bound or transmembrane protein. Transmembrane regions comprise at least the transmembrane region(s) of) the alpha, beta or zeta chain of the T-cell receptor, CD28, CD3, CD45, CD4, CDS, CD9, CD16, CD22; CD33, CD37, CD64, CD80, CD86, CD134, CD137 and CD154. In a specific embodiment, the transmembrane domain comprises the amino acid sequence of the CD28 transmembrane domain as shown in Table 2, A representative polynucleotide sequence coding for the CD28 transmembrane domain is shown in Table 1(SEQ ID NO:5).

A transmembrane domain may be synthetic or a variant of a naturally occurring transmembrane domain. In embodiments, synthetic or variant transmembrane domains comprise predominantly hydrophobic residues such as leucine and valine. In embodiments, a transmembrane domain can have at least about 80%, 85%, 90%, 95%, or 100% amino acid sequence identity with a transmembrane domain as shown in Tabie 2 or Table 6. Variant transmembrane domains preferably have a hydrophobic score of at least 50 as calculated by Kyte Doolittle.

A polynucleotide coding for a transmembrane domain can be readily prepared by synthetic or recombinant methods. In embodiments, a polynucleotide coding for a transmembrane domain is operably linked to a polynucleotide coding for a intracellular signaling region. In embodiments, the polynucleotide coding for a transmembrane domain may also have one or more restriction enzyme sites at the 5' and/or 3 ' ends of the coding sequence in order to provide for easy excision and replacement of the polynucleotide coding for a transmembrane domain with another polynucleotide coding for a different transmembrane domain. In embodiments, the polynucleotide coding for a transmembrane domain is codon optimized for expression in mammalian cells.

Intracellular signaling domain

In embodiments, the chimeric receptor nucleic acid comprises a

polynucleotide coding for an intracellular signaling domain. The intracellular signaling domain provides for activation of one function of the transduced cell expressing the chimeric receptor upon binding to the ligand expressed on tumor cells. In embodiments, the intracellular signaling domain contains one or more intracellular signaling domains. In embodiments, the intracellular signaling domain is a portion of and/or a variant of an intracellular signaling domain that provides for activation of at least one function of the transduced cell.

Examples of intracellular signaling domains for use in a chimeric receptor of the disclosure include the cytoplasmic sequences of the CD3 zeta chain, and/or co- receptors that act in concert to initiate signal transduction following chimeric receptor engagement, as well as any derivative or variant of these sequences and any synthetic sequence that has the same functional capability. T cell activation can be said to be mediated by two distinct classes of cytoplasmic signaling sequence: those that initiate antigen-dependent primary activation and provide a T cell receptor like signal (primary cytoplasmic signaling sequences) and those that act in an antigen- independent manner to provide a secondary or co-stimulatory signal (secondary cytoplasmic signaling sequences). Primary cytoplasmic signaling sequences that act in a stimulatory manner may contain signaling motifs which are known as receptor tyrosine-based activation motifs or ITAMs. Examples of ITAM containing primary cytoplasmic signaling sequences include those derived from CD3 zeta, FcR gamma, CD3 gamma, CD3 delta, CD3 epsilon, CDS, CD22, CD79a, CD79b, and CD66d. In embodiments, the primary signaling intracellular domain can have at least about 80%, 85%, 90%, or 95% sequence identity to CD3zeta having a sequence provided in Table 2. In embodiments variants, of CD3 zeta retain at least one, two, three or all ITAM regions as shown in Table 7.

In a preferred embodiment, the intracellular signaling domain of the chimeric receptor can be designed to comprise the CD3-zeta signaling domain by itself or combined with any other desired cytoplasmic domain(s). For example, the intracellular signaling domain of the chimeric receptor can comprise a CD3zeta chain and a costimulatory signaling region.

The costimulatory signaling region refers to a portion of the chimeric receptor comprising the intracellular domain of a costimulatory molecule. A costimulatory molecule is a cell surface molecule other than an antigen receptor or their ligands that is required for a response of lymphocytes to an antigen. Examples of such molecules include CD27, CD28, 4-1 BB (CD 137), OX40, CD30, CD40, lymphocyte function-associated antigen-1 (LFA-1), CD2, CD7, LIGHT, NKG2C, B7-H3, and a ligand that specifically binds with CD83. In embodiments, the costimulatory signaling domain can have at least about 80%, 85%, 90%, or 95% amino acid sequence identity to the intracellular domain of CD28 as shown in Table 5 or to 4-1BB having a sequence provided in Table 2, In an embodiment, a variant of the CD28 intracellular domam comprises an amino acid substitution at positions 186-187, wherein LL is substituted with GG.

The intracellular signaling sequences of the chimeric receptor may be linked to each other in a random or specified order. Optionally, a short oligo- or polypeptide linker, preferably between 2 and 10 amino acids in length may form the linkage. In one embodiment, the intracellular signaling domains comprises all or a portion of the signaling domain of CD3-zeta or variant thereof and all or a portion of the signaling domain of CD28 or a variant thereof. In another embodiment, the intracellular signaling domain comprises all or a portion of the signaling domain of CD3-zeta or variant thereof and all or a portion of the signaling domain of 4-1BB or variant thereof. In yet another embodiment, the intracellular signaling domain comprises all or a portion of the signaling domain of CD3-zeta or variant thereof, all or a portion of the signaling domain of CD28 or variant thereof, and all or a portion of the signaling domain of 4-IBB or variant thereof. In a specific embodiment, the amino acid sequence of the intracellular signaling domain comprising a variant of CD3zeta and a portion of the 4-IBB intracellular signaling domain is provided in Table 2. A representative nucleic acid sequence is provided in Table 1(SEQ ID NO:6; SEQ ID NO:7).

In an embodiment, a polynucleotide coding for an intracellular signaling domain comprises a 4- IBB intracellular domain linked to a portion of a CD3zeta domain. In other embodiments, a 4-IBB intracellular domain and a CD28 intracellular domain are linked to a portion of a CD3 zeta domain.

A polynucleotide coding for an intracellular signaling domain can be readily prepared by synthetic or recombinant methods from the amino acid. sequence. In embodiments, the polynucleotide coding for an intracellular signaling domain may also have one or more restriction enzyme sites at the 5' and/or 3' ends of the coding sequence in order to provide for easy excision and replacement of the polynucleotide coding for an intracellular signaling domain with another polynucleotide coding for a different intracellular signaling domain. In embodiments, the polynucleotide coding for an intracellular signaling domain is codon optimized for expression in mammalian cells. Marker sequences

In embodiments, the chimeric receptor nucleic acid optionally further comprises a polynucleotide sequence coding for a marker sequence. A marker sequence can provide for selection of transduced cells, and identification of transduced cells. In embodiments, the marker sequence is operably linked to a polynucleotide sequence coding for a linker sequence. In embodiments, the linker sequence is a cleavable linker sequence.

A number of different marker sequences can be employed. Typically a marker sequence has a functional characteristic that allows for selection of transduced cells and/or detection of transduced cells. In embodiments, the marker sequence is compatible with transduction of human lymphocytes.

The positive selectable marker may be a gene, which upon being introduced into the host cell, expresses a dominant phenotype permitting positive selection of cells carrying the gene. Genes of this type are known in the art, and include, inter alia, hygromycin-B phosphotransferase gene (hph) which confers resistance to hygromycin B, the amino glycoside phosphotransferase gene (neo or aph) from Tn5 which codes for resistance to the antibiotic G41 8, the dihydrofolate reductase (DHF ) gene, the adenosine deaminase gene (ADA), and the multi-drug resistance (MDR) gene.

In an embodiment, a chimeric receptor nucleic acid further comprises a polynucleotide coding for a marker sequence. In an embodiment, the marker sequence is a truncated epidermal growth factor receptor as shown in Table 2, An exemplary polynucleotide for the truncated epidermal growth factor receptor is shown in Table 1. (SEQ ID IMO:9)In embodiments, the polynucleotide coding for the marker sequence is operably linked to a polynucleotide coding for a linker sequence. In a specific embodiment, the linker sequence is a cleavable linker sequence T2A as shown in Table 2. An exemplary polynucleotide sequence coding for the T2A tinker is provided in Table 1 .(SEQ ID NO:8)

A polynucleotide coding for marker sequence can be readily prepared by synthetic or recombinant methods from the amino acid sequence. In embodiments a polynucleotide coding for a marker sequence is operably linked to a polynucleotide coding for an intracellular signaling domain. In embodiments, the polynucleotide coding for a marker sequence may also have one or more restriction enzyme sites at the 5' and/or 3 ' ends of the coding sequence in order to provide for easy excision and replacement of the polynucleotide coding for a marker sequence with another polynucleotide coding for a different marker sequence. In embodiments, the polynucleotide coding for a marker sequence is cod on optimized for expression in mammalian cells.

Vectors, Cells and Methods of transducing cells

Selection and Sorting of T lymphocyte populations

The compositions described herein provide for CD4+ and/or CD8+ T lymphocytes. T lymphocytes can be collected in accordance with known techniques and enriched or depleted by known techniques such as affinity binding to antibodies such as flow cytometry and/or immunomagnetic selection. After enrichment and/or depletion steps, in vitro expansion of the desired T lymphocytes can be carried out in accordance with known techniques (including but not limited to those described in US Patent No. 6,040,1 77 to Riddell et al.), or variations thereof that will be apparent to those skilled in the art. In embodiments, the T cells are autologous T cells obtained from the patient.

For example, the desired T cell population or subpopulation may be expanded by adding an initial T lymphocyte population to a culture medium in vitro, and then adding to the culture medium feeder cells, such as non-dividing peripheral blood mononuclear cells (PBMC), (e.g., such that the resulting population of cells contains at least about 5, 10, 20, or 40 or more PBMC feeder cells for each T lymphocyte in the initial population to be expanded); and incubating the culture (e.g. for a time sufficient to expand the numbers of T cells). The non-dividing feeder cells can comprise gamma- irradiated PBMC feeder cells. In some embodiments, the PBMC are irradiated with gamma rays in the range of about 3000 to 3600 rads to prevent cell division. The order of addition of the T cells and feeder cells to the culture media can be reversed if desired. The culture can typically be incubated under conditions of temperature and the like that are suitable for the growth of T lymphocytes. For the growth of human T lymphocytes, for example, the temperature will generally be at least about 25 degrees Celsius, preferably at least about 30 degrees, more preferably about 37 degrees.

The T lymphocytes expanded include CD8 ⁺ cytotoxic T lymphocytes (CTL) and CD4 ⁺ helper T lymphocytes that may be specific for an antigen present on a human tumor or a pathogen.

Optionally, the expansion method may further comprise the step of adding non-dividing EBV-transformed lymphoblastoid cells (LCL) as feeder cells. LCL can be irradiated with gamma rays in the range of about 6000 to 10,000 rads. The LCL feeder cells may be provided in any suitable amount, such as a ratio of LCL feeder cells to initial T lymphocytes of at least about 10: 1.

Optionally, the expansion method may further comprise the step of adding anti-CD3 and/or anti CD28 antibody to the culture medium (e.g., at a concentration of at least about 0.5 ng/ml). Optionally, the expansion method may further comprise the step of adding 1L-2 and/or IL-1 5 to the culture medium (e.g., wherein the concentration of IL-2 is at least about 10 units/m l).

After isolation of T lymphocytes both cytotoxic and helper T lymphocytes can be sorted into naive, memory, and effector T cell subpopulations either before or after expansion.

CD8+ cells can be obtained by using standard methods. In some

embodiments, CD8+ cells are further sorted into naive, central memory, and effector memory cells by identifying cell surface antigens that are associated with each of those types of CD8+ cells. In embodiments, memory T cells are present in both CD62L-!- and CD62L- subsets of CD8+ peripheral blood lymphocytes. PBMC are sorted into CD62L-CD8+ and CD62L+CD8+ fractions after staining with anti-CD8 and anti-CD62L antibodies. In some embodiments, the expression of phenotypic markers of central memory T _CM include CD45RO, CD62L, CCR7, CD28, CD3, and CD 127 and are negative or low for granzyme B. In some embodiments, central memory T cells are CD45RO+, CD62L+, CD8+ T cells. In some embodiments, effector T _E are negative for CD62L, CCR7, CD28, and CD 127, and positive for granzyme B and perforin. In some embodiments, na ^' ive CD8+ T lymphocytes are characterized by the expression of phenotypic markers of na ^' ive T cells including CD62L, CCR7, CD28, CD3, CD127, and CD45RA.

Whether a cell or cell population is positive for a particular cell surface marker can be determined by flow cytometry using staining with a specific antibody for the surface marker and an isotype matched control antibody. A cell population negative for a marker refers to the absence of significant staining of the cell population ith the specific antibody above the isotype control, positive refers to uniform staining of the cell population above the isotype control. In some embodiments, a decrease in expression of one or markers refers to loss of 1 loglO in the mean fluorescence intensity and/or decrease of percentage of cells that exhibit the marker of at least about 20% of the cells, 25% of-the cells, 30% of the cells, 35% of the cells, 40% of the cells, 45% of the cells, 50% of the cells, 55% of the cells, 60% of the cells, 65% of the cells, 70% of the cells, 75% of the cells, 80% of the cells, 85% of the cells, 90% of the cell, 95% of the cells, and 100% of the cells and any % between 20 and 100% when compared to a reference cell population. In some embodiments, a cell population positive for one or markers refers to a percentage of cells that exhibit the marker of at least about 50% of the cells, 55% of the cells, 60% of the cells, 65% of the cells, 70% of the cells, 75% of the cells, 80% of the cells, 85% of the cells, 90% of the cell, 95% of the cells, and 100% of the cells and any % between 50 and 100% when compared to a reference cell population.

CD4+ T helper cells are sorted into nai e, central memory, and effector cells by identifying cell populations that have cell surface antigens. CD4+ lymphocytes can be obtained by standard methods. Tn some embodiments, naive CD4+ T lymphocytes are CD45RO-, CD45RA+, CD62L+, CD4+ T cells. In some embodiments, central memory CD4+ cells are CD62L+ and CD45RO+. In some embodiments, effector CD4+ cells are CD62L- and CD45RO-.

In embodiments, populations of CD4+ and CD8+ that are antigen specific can be obtained by stimulating naive or antigen specific T lymphocytes with antigen. For example, antigen-specific T celt lines or clones can be generated to

Cytomegalovirus antigens by isolating T cells from infected subjects and stimulating the cells in vitro with the same antigen. Naive T cells may also be used. Any number of antigens from tumor cells may be utilized as targets to elicit T cell responses. In some embodiments, the adoptive cellular immunotherapy

compositions are useful in the treatment of a disease or disorder including a solid tumor, hematologic malignancy, breast cancer or melanoma.

Modification of T lymphocyte populations

In some embodiments it may be desired to introduce functional genes into the T cells to be used in immunotherapy in accordance with the present disclosure. For example, the introduced gene or genes may improve the efficacy of therapy by promoting the viability and/or function of transferred T cells; or they may provide a genetic marker to permit selection and/or evaluation of in vivo survival or migration; or they may incorporate functions that improve the safety of immunotherapy, for example, by making the cell susceptible to negative selection in vivo as described by Lupton S. D. et al., Mol. and Cell Biol., 11 :6 (1991); and Riddell et al., Human Gene Therapy 3 :319-338 (1992); see also the publications of PCT/US91/08442 and PCT/US94/05601 by Lupton et al. describing the use of bi functional selectable fusion genes derived from fusing a dominant positive selectable marker with a negative selectable marker. This can be carried out in accordance with known techniques (see, e.g., US Patent No. 6,040,177 to Riddell et al. at columns 14-17) or variations thereof that will be apparent to those skilled in the art based upon the present disclosure.

In embodiments, T cells are modified with chimeric receptors as described herein. In some embodiments, the T cells are obtained from the subject to be treated, in other embodiments, the lymphocytes are obtained from allogeneic human donors, preferably healthy human donors.

In some embodiments, chimeric receptors comprise a Hgand binding domain that specifically binds to a tumor cell surface molecule, a polypeptide spacer region, a transmembrane domain and an intracellular signaling domain as described herein. In embodiments, the ligand binding domain is a single-chain antibody fragment

(scFv) that is derived from the variable heavy (VH) and variable light (VL) chains of a monoclonal antibody (m Ab). Costimulatory signals can also be provided through the chimeric receptor by fusing the costimulatory domain of CD28 and/or 4- I BB to the CD3ζ chain. Chimeric receptors are specific for cell surface molecules independent from HLA, thus overcoming the limitations of TCR-recognition including HLA-restriction and low levels of HLA-expression on tumor cells.

Chimeric receptors can be constructed with a specificity for any cell surface marker by utilizing antigen binding fragments or antibody variable domains of, for example, antibody molecules. The antigen binding molecules can be linked to one or more cell signaling modules. In embodiments, cell signaling modules include CD3 transmembrane domain, CD3 intracellular signaling domains, and CD28 transmembrane domains. In embodiments, the intracellular signaling domain comprises a CD28 transmembrane and signaling domain linked to a CD3 zeta intracellular domain. In some embodiments, a chimeric receptor can also include a transduction marker such as tEGFR.

In embodiments, the same or a different chimeric receptor can be introduced into each of population of CD4+ and CD8+ T lymphocytes. In embodiments, the chimeric receptor in each of these populations has a ligand binding domain that specifically binds to the same ligand on the tumor or infected cell. The cellular signaling modules can differ. In embodiments, the intracellular signaling domain of the CD8+ cytotoxic T cells is the same as the intracellular signaling domain of the CD4+ helper T cells. In other embodiments, the intracellular signaling domain of the CD8+ cytotoxic T cells is different than the intracellular signaling domain of tire CD4+ helper T cells.

In embodiments each of the CD4 or CD8 T lymphocytes can be sorted in to naive, central memory, effector memory or effector cells prior to transduction as described herein. In alternative embodiments, each of the CD4 or CD8 T lymphocytes can be sorted in to na ^' ive, central memory, effector memory, or effector cells after transduction.

Various transduction techniques have been developed which utilize recombinant infectious virus particles for gene delivery. This represents a currently preferred approach to the transduction of T lymphocytes of the present invention. The viral vectors which have been used in this way include virus vectors derived from simian virus 40, adenoviruses, adeno-associated virus (AAV), lentiviral vectors, and retroviruses. Thus, gene transfer and expression methods are numerous but essentially function to introduce and express genetic material in mammalian cells. Several of the above techniques have been used to transduce hematopoietic or lymphoid cells, including calcium phosphate transfection, protoplast fusion, electroporation, and infection with recombinant adenovirus, adeno-associated virus and retrovirus vectors. Primary T lymphocytes have been successfully transduced by electroporation and by retroviral or lentiviral infection.

Retroviral and lentiviral vectors provide a highly efficient method for gene transfer into eukaryotic cells. Moreover, retroviral or lentiviral integration takes place in a controlled fashion and results in the stable integration of one or a few copies of the new genetic information per cell.

It is contemplated that overexpression of a stimulatory factor (for example, a lymphokine or a cytokine) may be toxic to the treated individual. Therefore, it is within the scope of the invention to include gene segments that cause the T cells of the invention to be susceptible to negative selection in vivo. By "negative selection" is meant that the infused cell can be eliminated as a result of a change in the in vivo condition of the individual. The negative selectable phenotype may result from the insertion of a gene that confers sensitivity to an administered agent, for example, a compound. Negative selectable genes are known in the art, and include, inter alia the following: the Herpes simplex virus type I thymidine kinase (HSV-1 TK) gene, which confers ganciclovir sensitivity; the cellular hypoxanthine

phosphribosyltransferase (HP T) gene, the cellular adenine

phosphoribosyltransferase (APRT) gene, and bacterial cytosine deaminase,

In some embodiments it may be useful to include in the T cells a positive marker that enables the selection of cells of the negative selectable phenotype in vitro. The positive selectable marker may be a gene that upon being introduced into the host cell expresses a dominant phenotype permitting positive selection of cells carrying the gene. Genes of this type are known in the art, and include, inter alia, hygromycin-B phosphotransferase gene (hph) which confers resistance to hygromycin B, the amino glycoside phosphotransferase gene (neo or aph) from Tn5 which codes for resistance to the antibiotic G418, the dihydrofolate reductase (DHF ) gene, the adenosine deaminase gene (ADA), and the multi-drug resistance (MDR) gene.

A variety of methods can be employed for transducing T lymphocytes, as is well known in the art. In embodiments, transduction is carried out using lentiviral vectors.

In embodiments, CD4+ and CD8+ cells each can separately be modified with an expression vector encoding a chimeric receptor to form defined populations. In embodiments, these cells are then further sorted into subpopuiations of na ^' ive, central memory and effector cells as described above by sorting for cell surface antigens unique to each of those cell populations. In addition, CD4+ or CD8+ cell populations may be selected by their cytokine profile or proliferative activities. For example, CD4+ T lymphocytes that have enhanced production of cytokines such as IL-2, IL-4, IL- 10, TNF , and IFNy as compared to sham transduced cells or transduced CD8+ cells when stimulated with antigen can be selected. In other embodiments, nai ^' ve or central memory CD4+ T cells that have enhanced production of IL-2 and/or TNFa are selected. Likewise, CD 8+ cells that have enhanced IFNy production are selected as compared to sham transduced CD 8+ cells.

In embodiments, CD4+ and CD8+cells that proliferate in response to antigen or tumor targets are selected. For example, CD4+ cells that proliferate vigorously when stimulated with antigen or tumor targets as compared to sham transduced cells, or CD8+ transduced cells are selected. In some embodiments, CD4+ and CD8+ cells are selected that are cytotoxic for antigen bearing cells. In embodiments, CD4+ are expected to be weakly cytotoxic as compared to CD8+ cells.

In a preferred embodiment, transduced lymphocytes, such as CD8+ central memory cells, are selected that provide for tumor cell killing in vivo using an animal model established for the particular type of cancer. Such animal models are known to those of skill in the art and exclude human beings. As described herein, not all chimeric receptor constructs transduced into lymphocytes confer the ability to kill tumor cells in vivo despite the ability to become activated and kill tumor cells in vitro. In particular, for some target molecules T cells having chimeric receptor constructs with a long spacer region were less effective at killing tumor cells in vivo as compared to T cells having a chimeric receptor with short spacer region. For other target molecules, T cells having chimeric receptor constructs with a short spacer region were less effective at killing tumor cells in vivo as compared to T cells having chimeric receptors with a long spacer region.

In yet other embodiments, transduced chimeric receptor expressing T cells are selected that can persist in vivo using an animal model established for the particular type of cancer. In embodiments, transduced chimeric receptor CD8+ centra] memory cells with a short spacer region have been shown to persist in vivo after introduction into the animal for about 3 day or more, 10 days or more, 20 days or more, 30 days or more, 40 days or more, or 50 days or more.

The disclosure contemplates that combinations of CD4+ and CD8+ T cells will be utilized in the compositions. In one embodiment, combinations of chimeric receptor transduced CD4+ cells can be combined with chimeric receptor transduced CD8+ cells of the same ligand specificity or combined with CD8 ⁺ T cells that are specific for a distinct tumor ligand. In other embodiments, chimeric receptor transduced CD8+ cells are combined with chimeric receptor transduced CD4+ cells specific for a different ligand expressed on the tumor. In yet another embodiment, chimeric receptor modified CD4+ and CD 8+ cells are combined. In embodiments CD8+ and CD4+ cells can be combined in different ratios for example, a 1 : 1 ratio of CD8+ and CD4+, a ratio of 10: 1 of CD8+ to CD4+, or a ratio of 100:1 of CD8+ to CD4+. In embodiments, the combined population is tested for cell proliferation in vitro and/or in vivo, and the ratio of cells that provides for proliferation of cells is selected.

As described herein, the disclosure contemplates that CD4+ and CD8+ cells can be further separated into subpopulations, such as naive, central memory, and effector memory cell populations. As described herein, in some embodiments, naive CD4+ cells are CD45RO-, CD45RA+, CD62L+, CD4+ positive T cells. In some embodiments, central memory CD4+ cells are CD62L positive and CD45RO positive. In some embodiments, effector CD4+ cells are CD62L negative and CD45RO positive. Each of these populations may be independently modified with a chimeric receptor.

As described herein, in embodiments, memory T cells are present in both CD62L+ and CD62L- subsets of CD8+ peripheral blood lymphocytes. PBMC are sorted into CD62L-CD8+ and CD62L+CD8+ fractions after staining with anti-CD8 and anti-CD62L antibodies. In some embodiments, the expression of phenotypic markers of central memory T cells (TCM) include CD62L, CCR7, CD28, CD3, and CD127 and are negative or low for granzyme B. In some embodiments, central memory T cells are CD45RO+, CD62L+, CD8+ T cells. In some embodiments, effector T cells (TE) are negative for CD62L, CCR7, CD28, and CD127, and positive for granzyme B and perforin. In some embodiments, na ^' ive CD8+ T lymphocytes are characterized by CD8+, CD62L+, CD45RO+, CCR7+, CD28+ GDI 27+, and CD45RO+. Each of these populations may be independently modified with a chimeric receptor .

After transduction and/or selection for chimeric receptor bearing cells, the cell populations are preferably expanded in vitro until a sufficient number of cells are obtained to provide for at least one infusion into a human subject, typically around 10 ⁴ cells/kg to 10 ⁹ cells/kg In embodiments, the transduced cells are cultured in the presence of antigen bearing cells, anti CD3, anti CD28, and IL 2, IL-7, IL 15, IL-21 and combinations thereof. Each of the subpopulations of CD4+ and CD8+ cells can be combined with one another. In a specific embodiment, modified naive or central memory CD4+ cells are combined with modified central memory CD8+ T cells to provide a synergistic cytotoxic effect on antigen bearing cells, such as tumor cells.

Compositions

The disclosure provides for an adoptive cellular immunotherapy composition comprising a genetically modified T lymphocyte cell preparation as described herein.

In embodiments, the T lymphocyte cell preparation comprises CD4 ÷ T cells that have a chimeric receptor comprising an extracellular antibody variable domain specific for a ligand associated with the disease or disorder, a customizable spacer region, a transmembrane domain, and an intracellular signaling domain of a T cell receptor or other receptors as described herein. In other embodiments, an adoptive cellular immunotherapy composition further comprises a chimeric receptor modified tumor-specific CD8+ cytotoxic T lymphocyte cell preparation that provides a cellular immune response, wherein the cytotoxic T lymphocyte cell preparation comprises CD8+ T cells that have a chimeric receptor comprising an extracellular single chain antibody specific for a ligand associated with the disease or disorder, a customizable spacer regipn, a transmembrane domain, and an intracellular signaling domain of a T cell receptor as described herein. In embodiments, the chimeric receptor modified T cell population of the disclosure can persist in vivo for at least about 3 days or longer.

In some embodiments, an adoptive cellular immunotherapy composition comprises a chimeric receptor modified tumor-specific CD8+ cytotoxic T lymphocyte cell preparation that provides a cellular immune response, wherein the cytotoxic T lymphocyte cell preparation comprises CD8+ T cells that have a chimeric receptor comprising an extracellular single chain antibody specific for a ligand associated with the disease or disorder, a customizable spacer region, a transmembrane domain, and an intracellular signaling domain of a T cell receptor, in combination with an antigen-reactive chimeric receptor modified na ^' ive CD4+ T helper cell derived from CD45RO- CD62L+ CD4+ T cells, and a pharmaceutically acceptable carrier. In other embodiments, an adoptive cellular immunotherapy composition comprises an antigen specific CD8+ cytotoxic T lymphocyte cell preparation that provides a cellular immune response derived from the patient combined with an antigen-reactive chimeric receptor modified naive CD4+ T helper cell that augments the CD8+ immune response, wherein the helper T lymphocyte cell preparation comprises CD4 + T cells that have a chimeric receptor comprising an extracellular antibody variable domain specific for the antigen associated with the disease or disorder, a customizable spacer region, a transmembrane domain, and an intracellular signaling domain of a T cell receptor.

In a further embodiment, an adoptive cellular immunotherapy composition comprises an antigen-reactive chimeric receptor modified naive CD4+ T helper cell that augments the CD8+ immune response, wherein the helper T lymphocyte cell preparation comprises CD4 + T cells that have a chimeric receptor comprising an extracellular antibody variable domain specific for a ligand associated with a disease or disorder, a customizable spacer region, a transmembrane domain, and an intracellular signaling domain of a T cell receptor.

In embodiments, the CD4+ T helper lymphocyte cell is selected from the group consisting of naive CD4+ T cells, central memory CD4+ T cells, effector memory CD4+ T cells, or bulk CD4+ T cells. In some embodiments, CD4+ helper lymphocyte cell is a naive CD4+ T cell, wherein the naive CD4+ T cell comprises a CD45RO, CD45RA+, CD62L+ CD4+ T cell. In embodiments, the CD8+ T cytotoxic lymphocyte cell is selected from the group consisting of naive CD8+ T cells, central memory CD8+ T cells, effector memory CD8+ T cells or bulk CD8+ T cells. In some embodiments, the CD8+ cytotoxic T lymphocyte cell is a central memory T cell wherein the central memory T cell comprises a CD45RO+, CD62L+, CD8+ T cell. In yet other embodiments, the CD8+ cytotoxic T lymphocyte cell is a central memory T cell and the CD4+ helper T lymphocyte cell is a naive or central memory CD4+ T cell.

Methods

The disclosure provides methods of making adoptive immunotherapy compositions and uses or methods of using these compositions for performing cellular immunotherapy in a subject having a disease or disorder. In embodiments, the chimeric receptor modified T cells as described herein are able to persist in vivo for at least 3 days, or at least 10 days. In embodiments, the chimeric receptor modified T cells as described herein can proliferate in vivo through at least 2, or at least 3 generations as determined by CFSE dye dilution. Proliferation and persistence of the chimeric receptor modified T cells can be determined by using an animal model of the disease or disorder and administering the cells and determining persistence and/ or proliferative capacity of the transferred cells. In other embodiments, proliferation and activation can be tested in vitro by going through multiple cycles of activation with antigen bearing cells.

In embodiments, a method of manufacturing the compositions comprises obtaining a modified naive CD4+ T helper cell, wherein the modified helper T lymphocyte cell preparation comprises CD4+ T cells that have a chimeric receptor comprising a ligand binding domain specific for a tumor cell surface molecule, a customized spacer domain, a transmembrane domain, and an intracellular signaling domain as described herein.

Tn another embodiment, a method further comprises obtaining a modified

CD8+ cytotoxic T cell, wherein the modified cytotoxic T lymphocyte cell preparation comprises CD8+ cells that have a chimeric receptor comprising a ligand binding domain specific for a tumor cell surface molecule, a customized spacer domain, a transmembrane domain, and an intracellular signaling domain as described herein.

In another embodiment, a method comprises obtaining a modified CD8+ cytotoxic T cell, wherein the modified cytotoxic T lymphocyte cell preparation comprises CD8+ T cells that have a chimeric receptor comprising a ligand binding domain specific for a tumor cell surface molecule, a customized spacer domain, a transmembrane domain, and an intracellular signaling domain as described herein, and further comprising combining the modified CD8+ cytotoxic T cells with a CD4+ helper cell lymphocyte cell preparation.

The preparation of the CD4+ and CD8+ cells that are modified with a chimeric receptor has been described above as well as in the examples. Antigen specific T lymphocytes can be obtained from a patient having the disease or disorder or can be prepared by in vitro stimulation of T lymphocytes in the presence of antigen. Subpopulattons of CD4+ and CD8+ T lymphocytes that are not selected for antigen specificity can also be isolated as described herein and combined in the methods of manufacturing. In embodiments, the combination of cell populations can be evaluated for uniformity of cell surface makers, the ability to proliferate through at least two generations, to have a uniform cell differentiation status. Quality control can be performed by coculturing an cell line expressing the target ligand with chimeric receptor modified T cells to determine if the chimeric receptor modified T cells recognize the cell line using cytotoxicity, proliferation, or cytokine production assays that are known in the field. Cell differentiation status and cell surface markers on the chimeric receptor modified T cells can be determined by flow cytometry. Tn embodiments, the markers and cell differentiation status on the CD8+ cells include CD3, CD8, CD62L, CD28, CD27, CD69, CD25, PD-1, CTLA-4, CD45RO, and CD45RA. In embodiments, the markers and the cell differentiation status on the CD4+ cells include CD3, CD4, CD62L, CD28, CD27, CD69, CD25, PD-1, CTLA-4 CD45RO, and CD45RA.

In embodiments, a method of selecting a spacer region for a chimeric receptor is provided herein. Surprisingly some chimeric receptor constructs, although effective to activate T cells in vitro, were not effective in vivo. In embodiments, a method comprises providing a plurality of chimeric receptor nucleic acids, wherein the chimeric receptor nucleic acids differ only in the spacer region; introducing each of the chimeric receptor nucleic acids into a separate T lymphocyte population; expanding each separate lymphocyte population in vitro, and introducing each lymphocyte population into an animal bearing a tumor to determine the anti-tumor efficacy of each of the chimeric receptor modified T cells, and selecting a chimeric receptor that provides anti-tumor efficacy as compared to each of the other separate lymphocyte populations modified with each of the other chimeric receptor modified T cells.

Animal models of different tumors are known. Anti-tumor efficacy can be measured by identifying a decrease in tumor volume, by determining animal death, persistence of the genetically modified T cells in vivo, activation of genetically modified T cells (for example, by detecting an increase in expression of CD25 and/CD69), and/or proliferation of genetically modified T cells in vivo. In an embodiment, a chimeric receptor is selected that provides for the best anti-tumor efficacy in vivo as determined by one or more of these parameters. Lack of antitumor efficacy can be determined by lack of persistence of the genetically modified lymphocytes in vivo, animal death, an increase in apoptosis as measured by an increase in induction of caspase -3, and/or a decrease in proliferation of genetically modified lymphocytes.

In embodiments, providing a plurality of chimeric receptor nucleic acids, wherein the chimeric receptor nucleic acids differ only in the spacer region comprises providing a chimeric receptor construct comprising a polynucleotide coding for a ligand binding domain, wherein the ligand is a tumor specific antigen, viral antigen, or any other molecule expressed on a target cell population that is suitable to mediate recognition and elimination by a lymphocyte; a polynucleotide coding for a first polypeptide spacer having a defined restriction site at the 5' and 3 ' end of the coding sequence for the first polypeptide spacer; a polynucleotide coding for a transmembrane domain; and a polynucleotide coding for an intracellular signaling domain.

The disclosure also provides methods of performing cellular immunotherapy in a subject having a disease or disorder comprising: administering a composition of lymphocytes expressing a chimeric receptor as described herein. In other embodiments, a method comprises administering to the subject a genetically modified cytotoxic T lymphocyte cell preparation that provides a cellular immune response, wherein the cytotoxic T lymphocyte cell preparation comprises CD8 + T cells that have a chimeric receptor comprising a ligand binding domain specific for a tumor cell surface molecule, a customized spacer domain, a transmembrane domain, and an intracellular signaling domain as described herein, and a genetically modified helper T lymphocyte cell preparation that elicits direct tumor recognition and augments the genetically modified cytotoxic T lymphocyte cell preparations ability to mediate a cellular immune response, wherein the helper T lymphocyte cell preparation comprises CD4+ T cells that have a chimeric receptor comprising a ligand binding domain specific for a tumor cell surface molecule, a customized spacer domain, a transmembrane domain, and an intracellular signaling domain as described herein.

While not limiting the scope of the disclosure, it is believed by selecting the chimeric receptor modified T cell population that can persist and proliferate in vivo prior to administration may result in the ability to use a lower dose of T cells and provide more uniform therapeutic activity. Tn embodiments, the dose of T cells can be reduced at least 10%, 20%, or 30% or greater. Reduction in the dose of T cells may be beneficial to reduce the risk or tumor lysis syndrome and cytokine storm.

In another embodiment, a method of performing cellular immunotherapy hi subject having a disease or disorder comprises: administering to the subject a genetically modified helper T lymphocyte cell preparation, wherein the modified helper T lymphocyte cell preparation comprises CD4+ T cells that have a chimeric receptor comprising a ligand binding domain specific for a tumor cell surface molecule, a customized spacer domain, a transmembrane domain, and an intracellular signaling domain as described herein. In an embodiments, the method further comprises administering to the subject a genetically modified cytotoxic T lymphocyte cell preparation, wherein the modified cytotoxic T lymphocyte cell preparation comprises CD8+ cells that have a chimeric receptor comprising a ligand binding domain specific for a tumor cell surface molecule, a customized spacer domain, a transmembrane domain, and an intracellular signal ing domain as described herein.

Another embodiment describes a method of performing cellular

immunotherapy in a subject having a disease or disorder comprising: analyzing a biological sample of the subject for the presence of a target molecule associated with the disease or disorder and administering the adoptive immunotherapy compositions described herein, wherein the chimeric receptor specifically binds to the target molecule.

In some embodiments, the CD4+ T helper lymphocyte cell is selected prior to introduction of the chimeric receptor from the group consisting of naive CD4+ T cells, central memory CD4+ T cells, effector memory CD4+ T cells or bulk CD4+ T cells. In a specific embodiment, CD4+ helper lymphocyte cell is a naive CD4+ T cell, wherein the naive CD4+ T cell comprises a CD45RO-, CD45RA+, CD62L+ CD4+ T cell. In yet other embodiments, the CD 8+ T cytotoxic lymphocyte cell is selected prior to introduction of the chimeric receptor from the group consisting of naive CD8+ T cells, central memory CD8+ T cells, effector memory CD8+ T cells or built CD8+ T cells. In a specific embodiment, the CD8+ cytotoxic T lymphocyte cell is a central memory T cell wherein the central memory T cell comprises a CD45RO+, CD62L+, CD8+ T cell. Jn a specific embodiment, the CD8+ cytotoxic T lymphocyte cell is a central memory T cell and the CD4+ helper T lymphocyte cell is a naive CD4+ T cell.

In embodiments, the CD8+ T cell and the CD4+ T cell are both genetically modified with a chimeric receptor comprising an antibody heavy chain domain that specifically binds a tumor-specific cell surface molecule. In other embodiments, the intracellular signaling domain of the CD8 cytotoxic T cells is the same as the intracellular signaling domain of the CD4 helper T cells. In yet other embodiments, the intracellular signaling domain of the CD8 cytotoxic T cells is different than the intracellular signaling domain of the CD4 helper T cells.

Subjects that can be treated by the present invention are, in general, human and other primate subjects, such as monkeys and apes for veterinary medicine purposes. The subjects can be male or female and can be any suitable age, including infant, juvenile, adolescent, adult, and geriatric subjects.

The methods are useful in the treatment of, for example, hematologic malignancy, melanoma, breast cancer, and other epithelial malignancies or solid tumors. In some embodiments, the molecule associated with the disease or disorder is selected from the group consisting of orphan tyrosine kinase receptor ROR1 , Her2, CD19, CD20, CD22, mesothelin, CEA, and hepatitis B surface antigen.

Subjects that can be treated include subjects afflicted with cancer, including but not limited to colon, lung, liver, breast, renal, prostate, ovarian, skin (including melanoma), bone, and brain cancer, etc. In some embodiments the tumor associated antigens or molecules are known, such as melanoma, breast cancer, squamous cell carcinoma, colon cancer, leukemia, myeloma, and prostate cancer. In. other embodiments the tumor associated molecules can be targeted with genetically modified T cells expressing an engineered chimeric receptor. Examples include but are not limited to B cell lymphoma, breast cancer, prostate cancer, and leukemia.

Cells prepared as described above can be utilized in methods and compositions for adoptive immunotherapy in accordance with known techniques, or variations thereof that will be apparent to those skilled in the art based on the instant disclosure.

In some embodiments, the cells are formulated by first harvesting them from their culture medium, and then washing and concentrating the cells in a medium and container system suitable for administration (a "pharmaceutically acceptable" carrier) in a treatment-effective amount. Suitable infusion medium can be any isotonic medium formulation, typically normal saline, Normosol R (Abbott) or Plasma-Lyte A (Baxter), but also 5% dextrose in water or Ringer's lactate can be utilized. The infusion medium can be supplemented with human serum albumin, fetal bovine serum or other human serum components.

A treatment effective amount of cells in the composition is at least 2 cell subsets (for example, 1 CD8+ central memory T cell subset and 1 CD4+ helper T cell subset) or is more typically greater than 10 ² cells, and up to 10 ⁶ , up to and including 10 ⁸ or 10 ⁹ cells and can be more than 10 ¹⁰ cells. The number of cells will depend upon the ultimate use for which the composition is intended as will the type of cells included therein. For example, if cells that are specific for a particular antigen are desired, then the population will contain greater than 70%, generally greater than 80%, 85% and 90-95% of such cells. For uses provided herein, the cells are generally in a volume of a liter or less, can be 500 mis or less, even 250 mis or 100 mis or less. Hence the density of the desired cells is typically greater than 10 ⁴ cells/ml and generally is greater than 10 ⁷ cells/ml, generally 10 ⁸ cells/ml or greater. The clinically relevant number of immune cells can be apportioned into multiple infusions that cumulatively equal or exceed 10 ⁶ , 10 ⁷ , 10 ^s , 10 ⁸ , 10 ⁹ , 10 ^l ° or 10 ¹¹ cells.

In some embodiments, the lymphocytes of the invention may be used to confer immunity to individuals. By "immunity" is meant a lessening of one or more physical symptoms associated with a response to infection by a pathogen, or to a tumor, to which the lymphocyte response is directed. The amount of cells administered is usually in the range present in normal individuals with immunity to the pathogen. Thus, the cells are usually administered by infusion, with each infusion in a range of from 2 cells, up to at least 10 ⁶ to 3xl0 ¹⁰ cells, preferably in the range of at least 10 ⁷ to 10 ⁹ cells. The T cells may be administered by a single infusion, or by multiple infusions over a range of time. However, since different individuals are expected to vary in responsiveness, the type and amount of cells infused, as well as the number of infusions and the time range over which multiple infusions are given are determined by the attending physician, and can be determined by routine examination. The generation of sufficient levels of T lymphocytes (including cytotoxic T lymphocytes and/or helper T lymphocytes) is readily achievable using the rapid expansion method of the present invention, as exemplified herein. See, e.g., US Patent No. 6,040,177 to Riddell et al. at column 17.

In embodiments, the composition as described herein are administered intravenously, intraperitoneally, intratumorly, into the bone marrow, into the lymph node, and /or into cerebrospinal fluid. In embodiments, the chimeric receptor engineered compositions are delivered to the site of the tumor. Alternatively, the compositions as described herein can be combined with a corapound that targets the cells to the tumor or the immune system compartments and avoid sites such as the lung.

In embodiments, the compositions as described herein are administered with chemotherapeutic agents and/or immunosuppressants. In an embodiment, a patient is first treated with a chemotherapeutic agent that inhibits or destroys other immune cells followed by the compositions described herein. In some cases, chemotherapy may be avoided entirely.

The present invention is illustrated further in the examples set forth below. EXPERIMENTAL

Example I. Customizing spacer domain length and scFv affinity for optimal recognition of ROR1 with chimeric receptor modified T ce!ls

We constructed chimeric receptors specific for the ROR1 molecule that is expressed on a large number of human malignancies including chronic lymphocytic leukemia, mantle cell lymphoma, acute lymphoblastic leukemia, and breast, lung prostate, pancreas and ovarian cancer. The ROR1 chimeric receptors were designed from ROR1 specific scFVs with different affinities and containing extracellular IgG4-Fc spacer domains of different lengths. The ability of T- eel Is expressing each ROR-1 specific chimeric receptor to recognize RO l ⁺ hematopoietic and epithelial tumors in vitro, and to eliminate human mantle cell lymphoma engrafted into immunodeficient mice was analyzed.

Materials and Methods

Human subjects

Peripheral blood mononuclear cells (PBMC) were obtained from healthy donors and patients after written informed consent on research protocols approved by the Institutional Review Board of the Fred Hutchinson Cancer Research Center (FHCRC).

Cell lines The K562, Raji, JeKo-1, MDA-MB-231 , MDA-MB-468, and 293T cell lines were obtained from the American Type Culture Collection. Dr. Edns H. Warren (FHCRC) kindly provided the renal cell cancer lines FARP, TREP and RWL.

K562/ROR1 and Raji/RORl were generated by lentiviral transduction with the full- length RORl-gene. To derive JeKo-l/ffluc, native JeKo-1 cells were transduced with a lentiviral vector encoding t e. firefly luciferase ffluc)-g&os upstream of a T2A sequence and eGFP. The transduced JeKo-1 cells were sorted for eGFP expression, and expanded for in vivo experiments.

Immunophenotyping

PBMC and cell lines were stained with the following conjugated mAbs:

CD3, CD4, CD5, CD8, CD19, CD28, CD45RO, CD62L, CD314 (NKG2D), MICA/B and matched isotype controls (BD Biosciences). Propidium iodide (PI) staining was performed for live/dead cell discrimination. Cell surface expression of ROR1 was analyzed using a polyclonal goat anti-human-RORl antibody (R&D Systems).

Surface expression of 2A2 ROR1 chimeric receptor was analyzed using a polyclonal goat anti-mouse-IgG antibody (Fab-specific) (Jackson ImmunoResearch). Flow analyses were done on a FACSCanto®, sort-purifications on a FACSAriall® (Becton Dickinson) and data analyzed using Flow Jo® software (Treestar).

Vector construction and preparation of chimeric receptor encoding lentivirus ROR1 -specific and CD19-specific chimeric receptors were constructed using VL and VH chain segments of the 2A2, R12, and Rl 1 mAbs (ROR1 ) and FMC63 mAb (CD19). (V ariable region sequences for Rl 1 and R12 are provided in Yang et al, Plos One 6(6):e21018, June 15, 201 1 ) Each scFV was linked by a (G ₄ S) ₃ (SEQ ID NO: 12) peptide to a spacer domain derived from IgG4-Fc (Uniprot Database:

P01 861 ,SEQ ID NO: 13) comprising either 'Hinge-CH2-CH3' (229 AA, SEQ ID NO:), 'Hinge-CH3 ' (1 19 AA,SEQ ID NO:) or 'Hinge' only (12 AA,SEQ. ID NO:4) sequences (Figure 1). AH spacers contained a S->P substitution within the 'Hinge' domain located at position 108 of the native IgG4-Fc protein, and were linked to the 27 AA transmembrane domain of human CD28 (Uniprot: PI 0747, SEQ ID NO: 14) and to a signaling module comprising either (i) the 41 AA cytoplasmic domain of human CD28 with an LL- GG substitution located at positions 1 86-187 of the native CD28 protein (SEQ ID NO: 14)or (ii) the 42 AA cytoplasmic domain of human 4-lBB (Uniprot: Q07011, SEQ ID NO:15), each of which was linked to the 1 12 AA cytoplasmic domain of isoform 3 of human CD3ζ (Uniprot: P20963, SEQ ID NO; 16). The construct encoded a T2A ribosomal skip element (SEQ ID

NO:8))and a tEGFR sequence (SEQ ID NO:9) downstream of the chimeric receptor. Codon-optimized nucleotide sequences encoding each transgene were synthesized (Life Technologies) and cloned into the epHIV7 lentiviral vector

ROR1 -chimeric receptor, CD19-chimeric receptor or tEGFR- encoding lentiviruses were produced in 293T cells using the packaging vectors pCHGP-2, pCMV-Rev2 and pCMV-G, and Calphos® transfection reagent (Clontech).

Generation of T-cell lines expressing ROR1 and CD19-chimeric receptors CD8 ⁺ CD45RO ⁺ CD62L ⁺ central memory T-cells (T _CM ) or bulk CD4 ⁺ T- cells were sorted from PBMC of normal donors, activated with anti-CD3/CD28 beads (Life Technologies), and transduced on day 3 after activation by

centrifugation at 800 g for 45 min at 32°C with lentiviral supernatant (MOT = 3) supplemented with 1 μ£/ηϋ_, polybrene ( illipore). T-cells were expanded in RPMI with 10% human serum, 2 mM L-glutamine and 1% penicillin-streptomycin (CTL medium), supplemented with recombinant human IL-2 to a final concentration of 50 U/mL. The tEGFR ⁺ subset of each T-cell line was enriched by immunomagnetic selection with biotin-conjugated anti-EGFR mAb (lmClone Systems) and streptavidin-beads (Miltenyi). RORl-chimeric receptor and tEGFR control T-cells were expanded using a rapid expansion protocol (Riddell SR, Greenberg PD,The use of anti-CD3 and anti-CD28 monoclonal antibodies to clone and expand human antigen-specific T cells J Immunol Methods. 1990; 128(2): 189-201. Epub

1990/04/17.), and CD19-chimeric receptor modified T-cells were expanded by stimulation with irradiated (8,000 rad) B-LCL at a T-cell:LCL ratio of 1 :7. T-cells were cultured in CTL medium with 50 U/mL IL-2.

Cytotoxicity, cytokine secretion and proliferation assays

Target cells were labeled with ^{5 l} Cr (PerkinElmer), washed and incubated in triplicate at l-2xl0 ³ cells/well with effector chimeric receptor modified T-cells at various effector to target (E:T) ratios. Supernatants were harvested for γ-counting after a 4-hour incubation and specific lysis calculated using the standard formula. For analysis of cytokine secretion, 5x10 ⁴ T-cells were plated in triplicate with target cells at an E:T ratio of 1 : 1 (primary CLL), 2: 1 (Raji RORl ; JeKo-1), 4: 1 (K562/ROR1, K562/CD19 and K562) or 10:1 (MDA-MB-231), and IFN-γ, T F-a and IL-2 measured by ELISA or multiplex cytokine immunoassay (Luminex) in supernatant removed after 24-h incubation. In experiments blocking NKG2D signaling, anti-NKG2D (clone 1 D11), anti-MICA/B (clone 6D4, all from BD) and anti-ULBP (kindly provided by Dr. Veronika Groh, FHCRC) were used at saturating concentrations. For analysis of proliferation, T-cells were labeled with 0.2 μΜ carboxyfluorescein succinimidyl ester (CFSE, Invitrogen), washed and plated in triplicate with stimulator cells in medium without exogenous cytokines. After 72-h incubation, ceils were labeled with anti-CD8 mAb and PI, and analyzed by flow cytometry to assess cell division of live CD8 ⁺ T-cells.

Experiments in NOD/SCID/yc ' (NSG) mice

The Institutional Animal Chimeric receptor and Use Committee approved all mouse experiments. Six- to 8-week old female NOD.Cg-Prkdc ^scid T12rg ^SmlWjl /SzJ (NSG) mice were obtained from the Jackson Laboratory or bred in-house. Mice were injected with 0.5x10 ⁶ JeKo-l/ffluc tumor cells via tail vein and received a subsequent tail vein injection of chimeric receptor-modified or control T-cells.

For bio luminescence imaging of tumor growth, mice received intraperitoneal injections of luciferin substrate (Caliper Life Sciences) resuspended in PBS (15 μg g body weight). Mice were anesthetized with isoflurane and imaged using an Xenogen IVIS Imaging System (Caliper) 10, 12 and 14 minutes after the injection of luciferin in small binning mode at an acquisition time of 1 s to 1 min to obtain unsaturated images. Luciferase activity was analyzed using Living Image Software (Caliper) and the photon flux analyzed within regions of interest that encompassed the entire body or the thorax of each individual mouse.

Statistical analyses

Statistical analyses were performed using Prism Software (GraphPad®). Student's t-test was performed as a two-sided paired test with a confidence interval of 95% and results with a p-value of p<0.05 were considered significant. Statistical analysis of survival were done by log-rank testing and results with a p-value of p<0.05 considered significant.

Results

Truncating the spacer domain of the 2Λ2 RORl -chimeric receptor confers superior recognition ofRORl ⁺ tumors We previously reported the design of a ROR1 -specific chimeric receptor using the 2A2 scFV, which binds to an epitope in the NH2-terminal, membrane distal Ig-like/Frizzled portion of RORl-1. The initial 2A2 ROR1 -chimeric receptor had a long 229 AA spacer that included the ^c Hinge-CH2-CH3 ' region of IgG4-Fc, and incorporated CD28 costimulatory and Οΰ3ζ signaling domains (Hudecek M et al. Blood, 2010). This chimeric receptor conferred specific recognition of RORl ⁺ tumors, but we hypothesized that because of the membrane distal location of the ROR1 epitope, truncating the spacer domain might enhance tumor recognition and T-cell signaling. Therefore, we constructed 2 additional chimeric receptors in which the IgG4-Fc spacer domain was sequentially deleted to derive ^! Hinge-CH3 ' (1 19 AA, intermediate), and 'Hinge-only' (12 AA, short) variants. Each of the new receptors contained the identical 2A2 scFV, and CD28 and CD3ζ signaling modules. The transgene cassette included a truncated EGFR (tEGFR) to serve as a transduction, selection and in vivo tracking marker for chimeric receptor-modified T-cells.

We transduced purified CD8 ⁺ T _C M with the 2A2 ROR1 -chimeric receptors containing full length or truncated igG4-Fc spacers, and with a tEGFR control vector. The mean transduction efficiency was 15% (range 9-22%), and transgene- positive T-cells were enriched to uniform purity (>90%) on day 10 by selection for tEGFR expression, and expanded (Figure 2A). Surface expression of each of the chimeric receptors was confirmed by staining with F(ab)-specific antibodies (Figure 2A).

Analysis of the in vilro function of CD8 ⁺ T-cells modified to express each of the 2A2 ROR1 -chimeric receptors demonstrated that each receptor conferred specific lysis of JeKo-1 MCL and primary CLL cells that naturally express ROR1, and of K562 cells that had been transduced with ROR1, but did not confer recognition of control ROR1 ^" targets (Figure 2B). T-cells expressing the short 'Hinge-only' 2A2 ROR 1 -chimeric receptor had maximum cytolytic activity, and a hierarchy (short>interrnediate»long) of tumor lysis was clearly evident against all RORl ⁺ tumor targets (Figure 2B), illustrating the importance of spacer domain length on the recognition of RORl ⁺ tumor cel ls.

Anti-tumor efficacy of adoptive T-cell therapy correlates with proliferation and survival of transferred T-cells, which could be altered by signaling through the chimeric receptor. We used CFSE dilution assays to analyze proliferation of T-cells modified with each of the 2A2 ROR1 -chimeric receptors after engagement of Raj i RORl or CLL, and found that the short spacer construct promoted the greatest T-cell proliferation following stimulation (Figure 2C). To ensure that the enhanced proliferation was not associated with greater activation induced cell death (AICD), we also analyzed the proportion of 2A2 ROR 1 chimeric receptor modified T-ceils that stained with propidium iodide (PI) after stimulation with Raji/RORl and JeKo- 1 tumor cells. We detected a much lower frequency of PI ⁺ CD8 ⁺ T-cells in the T-cell line modified with the short (Raji/RORl : 17.2%/JeKo-l : 20.2%) compared to the intermediate (41 ,6%/42.4%) and long (44.5%/48.5%) spacers.

Quantitative analysis of cytokine production in response to stimulation with Raji/RORl and primary CLL cells showed production of IFN-γ, TNF-a and IL-2 by T-cells expressing each of the 2A2 ROR1 chimeric receptors. As observed in cytotoxicity assays, the short spacer construct was superior in mediating cytokine secretion after tumor recognition (Figure 2D). Thus, this analysis shows that truncating the extracellular IgG4-Fc spacer domain of the 2A2 ROR1 -chimeric receptor leads to a significant increase in cytotoxicity, proliferation and in vitro effector functions after tumor recognition.

The Rll scFv that is specific for a membrane proximal epitope in the ROR1 Kringle domain requires a long extracellular spacer domain.

We transduced purified CD8 ⁺ T cells with ROR1 -chimeric receptors containing the Rl 1 scFv that is specific for the Kringle domain of ROR1 and containing full length or truncated IgG4-Fc spacers (CH3 and hinge only). The transduction efficiency with each of the short (IgG4 hinge only), intermediate (IgG4 hinge/CH3) ₅ and long (IgG4 hinge/CH2/CH3) vectors was comparable (45-51 %) as measured by EGFR expression. (Figure 3A). T cells transduced with each of the vectors were assayed for cytolysis (Figure 3 B), proliferation (Figure 3C), and cytokine production (Figure 3D) in response to leukemia or lymphoma cells that did or did not express ROR1. As shown, only T cells transduced with the Rl 1 chimeric receptor containing a long spacer sequence were able to efficiently recognize ROR 1 + tumors and mediate effector functions.

ROR1 chimeric receptors derived from a mAb R12 with higher affinity than 2A2 mediate superior anti-tumor reactivity We next examined whether increasing the affinity of the scFV used to construct the RORl chimeric receptor might influence tumor recognition and T-cell function. We generated RORl -specific chimeric receptors from the mAb R12 that like 2A2, binds to an epitope in the NH2-terminal Ig/Frizzled domain of RORl but with >50-fold higher monovalent binding affinity.

R12 RORl chimeric receptors were constructed with both long and short TgG4-Fc spacers to determine whether the optimal spacer design for this higher affinity scFV differed from that for a lower affinity scFV. We found that similar to 2A2, the short spacer R12 RORl chimeric receptor conferred improved cytolytic activity, cytokine secretion and proliferation (data not shown), suggesting that the shorter spacer length provides superior spatial engagement of the T-ceil and RORl ⁺ target cell for T-cell activation.

We then designed R12 and 2A2 RORl chimeric receptors that contained an optimal (short) extracellular spacer, and either a CD28 or 4- IBB costimulatory domain in tandem with Οϋ3ζ (4 constructs) for comparison (Figure 4A.B). These RORl -chimeric receptor constructs were expressed in purified CD8 ⁺ TCM of healthy donors, and we confirmed equivalent transgene expression by fEGFR staining (Figure 5 A). T-cells modified with each of the 2A2 and R12 RORl -chimeric receptors specifically lysed K562/ROR1 and Raj i/RORl tumor cells with approximately equivalent efficiency (Figure 5B). However, analysis of cytokine production showed that the high affinity R12 ROR l chimeric receptors that contained CD28 or 4- IBB conferred significantly higher ΓΡΝ-γ, TNF- and IL-2 production compared to the corresponding 2A2 constructs (Figure 5C). We found that T-cells expressing chimeric receptors with a CD28 costimulatory domain produced more IFN-γ, TNF-a and IL-2 compared to those with 4- 1 BB.

Experiments to analyze the proliferation of RORl chimeric receptor T-cells showed a higher percentage of proliferating T-cells and a higher number of cell divisions in T-cells expressing the high affinity R12 RORl chimeric receptors with CD28 and 4- I BB domain compared to T-cells expressing the respective 2A2 counterparts (Figure 4D). There was more vigorous proliferation in T-cells that expressed chimeric receptors with a CD28 domain, consistent with higher IL-2 production induced by these receptors. There was a lower frequency of AICD as measured by PI staining in T-cell lines modified with R12 compared to 2A2 ROR 1 - chimeric receptors after stimulation with Raji/RORl and JeKo-1 tumor cells respectively (R12: 5.6%/6.9% vs. 2A2: 10%/9.65%). T-cell lines that expressed chimeric receptors with a CD28 domain also had lower AICD compared to 4- IBB in response to Raji/RORl and JeKo-1 tumor cells respectively (R12: 16.4%/18.4% vs, 2A2 38.1%/39.6%).

To determine if the enhanced function observed with R12 RORl chimeric receptors in CD8 ⁺ T-celis extended to CD4 ⁺ T-cells, we transduced bulk CD4 T- cells with the 2A2 and R12 RORl chimeric receptors containing the short spacer and CD28 costimulatory domain. In response to Raji RORl ⁺ tumor cells, CD4 ⁺ T- cells that expressed the high affinity R12 scFV produced higher levels of ΙΡΝ-γ,

TNF-α, IL-2, IL-4, and IL-10, and underwent greater proliferation than CD4 ⁺ T-cells that expressed 2A2 (Figure 5 A,B). Both cytokine production and proliferation was superior in CD4 ¹ compared to CD8 ⁺ T-cells modified with the same RORl chimeric receptors. In summary, our data demonstrate that tailoring both the length of the non-signaling extracellular chimeric receptor spacer domain and scFV affinity are independent parameters that affect the function of RORl-chimeric receptor T-cells. D8 ⁺ T-cells modified with a high affinity RORl chimeric receptor have comparable activity to a CD19 chimeric receptor against primary CLL in vitro RORl and CD1 are both uniformly expressed on all primary CLL (Figure 6A), however the absolute number of RORl -molecules per tumor cell is estimated to be 10-fold lower than that of CDI 9, which has been successfully targeted in clinical trials with CD 19 chimeric receptor T-cells. We compared recognition of primary CLL by CD8 ⁺ T-cells expressing the optimized R12 and 2A2 RORl chimeric receptors, and a CD19 chimeric receptor derived from the FMC63 scFV.

We used purified CD8 ⁺ T _C M for chimeric receptor-modification to provide a uniform cell product and each chimeric receptor contained a short IgG4-Fc 'Hinge- only' spacer and 4-1 BB costimulatory domain. We confirmed our CD19 chimeric receptor (lgG4 Hinge) was at least as and more effective in recognizing CD 19* tumors as a CDI 9 chimeric receptor with CD8a Hinge spacer and 4-1 BB costimulatory domain that is being used in ongoing clinical trials. (Figure 20). T cells expressing CD19 chimeric receptors with 4-1 BB and CD3zeta and a modified lgG4-Fc hinge exhibit superior in vitro and in vivo function compared to T cells expressing CDI chimeric receptors with 4- IBB and CD3zeta and a CD8 alpha hinge. In Figure 20D, in vivo antitumor activity of T cells expressing a CD19 chimeric receptor with an IgG4 Fc hinge (group 1) or CD8 alpha hinge (group 2) and T cells that express tEGFR alone (group 3) in NSG mice inoculated with Raji tumor cells expressing firefly luciferase (ffluc) were compared. Mice were imaged 17 days after tumor inoculation and 10 days after T cell inoculation. The data shows greater tumor burden in mice treated with control tEGFR T cells (group 3) or with CD19 chimeric receptor CDS alpha hinge T cells (group 2) compared with mice treated with CD19 chimeric receptor IgG4 Fc hinge T cells (group 1).

The cytolytic activity of R12 RORl chimeric receptor T-cells against primary tumor cells from multiple CLL patients (n=4) was higher compared to T- cells modified with the lower affinity 2A2 RORl chimeric receptor, and equivalent to the lysis observed with CD19 chimeric receptor T-cells (Figure 6B). Multiplex cytokine analysis showed nearly equivalent production of lFN-γ and TNF-a, but less IL-2 production by CD8 ⁺ T-cells expressing the R12 RORl compared with those expressing the CD19-chimeric receptor after co-culture with primary CLL '(Figure 6C). 2A2 RORl chimeric receptor T-cells produced lower amounts of all cytokines than R12 RORl chimeric receptor T-cells as noted previously. Cytokine production by all of the chimeric receptor-transduced T-cells after stimulation with CLL was substantially less than with Raji/RORl, which unlike CLL expresses both CD80 and CD86 that can engage CD28 expressed on chimeric receptor T-cells (Figure 6A, C).

We observed less proliferation of T-cells expressing the R12 and 2A2 RORl chimeric receptor compared to the CD19 chimeric receptor after stimulation with CLL (CD19>R12>2A2) (Figure 6D). We hypothesized that proliferation of CD8 ⁺ RORl chimeric receptor T-cells in response to CLL may be augmented in the presence of chimeric receptor-modified CD4 ⁺ T-cells because of their higher secretion of IL-2 compared to CD8 ⁺ TCM (Figure 4A; Figure 8A). To test this possibility, we performed in vitro co-culture experiments where CD4 ⁺ and CD8 T M were separately modified with the R12 RORl , 2A2 RORl and CD19 chimeric receptors respectively, enriched for chimeric receptor expression, and combined at a 1 : 1 ratio to ensure equivalent proportions of CD8 ⁺ and CD4 T-cells modified with each of the vectors. These cells were CFSE-labeled and stimulated with primary CLL. We observed a dramatic increase in proliferation of CD8 ^h R12 RORl chimeric receptor T-cells after addition of chimeric receptor-transduced, but not untransduced CD4 T-cells (Figure 8B). Notably, when provided with CD4-help, we observed equivalent proliferation of R12 RORl and CD19 chimeric receptor CD8 ⁺ T-cells in response to CLL, whereas proliferation of CD8 ⁺ T-cells expressing the lower affinity 2A2 RORl chimeric receptor remained less. Collectively, our data show that the high affinity R12 RORl chimeric receptor confers superior reactivity compared to 2A2 against primary CLL cells in vitro.

RORl-chimeric receptor T-cells mediate in vivo anti-tumor activity in a mouse model of systemic mantle cell lymphoma

It remained uncertain whether the superior in vitro activity of T-cells modified with the higher affinity R12 chimeric receptor would translate into improved anti-tumor activity in vivo, and how targeting RO l would compare to targeting CD19. To address these questions, we inoculated cohorts of

immunodeficient NSG mice with the human MCL line JeKo-l/ffluc by tail vein injection, and seven days later when tumor was disseminated, treated the mice with a single intravenous dose of R12 RORl, 2A2 RORl or CD19 chimeric receptor CD8 ⁺ T-cells. Control mice were treated with tEGFR T-cells or untreated. All chimeric receptors had the optimal short spacer and the 4-1BB costimulatory domain.

Untreated NSG/JeKo-1 mice developed a rapidly progressive systemic lymphoma necessitating euthanasia approximately 4 weeks after tumor inoculation (Figure 9A- C).

We observed tumor regression and improved survival in all mice treated with R12 RORl , 2A2 RORl and CD19 chimeric receptor T-cells. Mice treated with R12 RORl chimeric receptor T-cells had a superior anti-tumor response and survival compared to mice treated with 2A2 RORl chimeric receptor T-cells (p<0.01), and comparable anti-tumor activity to mice treated with CD19 chimeric receptor T-cells (Figure 9A-C).

We analyzed the frequency of chimeric receptor T-cells in the peripheral blood following adoptive transfer and detected higher numbers of tEGFR * T-cells in mice treated with the R12 RORl chimeric receptor compared to the 2A2 RORl c imeric receptor, suggesting more vigorous proliferation in vivo improved tumor control. To confirm this, we administered CFSE-labeled CD19 chimeric receptor, R 12 and 2A2 RORl chimeric receptor T-cells to cohorts of NSG mice bearing JeKo-l /ffiuc, and analyzed T-cell proliferation in the peripheral blood, bone marrow and spleen 72 hours after transfer. A higher percentage of the R12 and CD19 chimeric receptor T-cells proliferated and underwent a greater number of cell divisions compared to 2A2 RORl chimeric receptor T-cells (Figure 9D). The JeKo- 1 tumor eventually recurred in all mice treated with RORl or CD19 chimeric receptor T-cells (Figure 9A-C). Tumor recurrence was not a result of the selection of RORl or CD 19 loss variants, as recurrent tumors were positive for both molecules.

For comparison, we analyzed anti-tumor efficacy of CD 19 chimeric receptor T-cells in NSG mice engrafted with Raji tumors and observed complete tumor eradication, indicating the recurrence of JeKo-1 reflects difficulty eradicating this tumor (data not shown). In summary, this data is the first to show that RORl chimeric receptor T-cells have anti-tumor efficacy in vivo, and suggest that for B- cell malignancies, an optimized RORl chimeric receptor such as R12 may be effective and spare normal CD19 ⁺ B-cells that lack RORl expression.

T-cells expressing the R12 RORl chimeric receptor have superior reactivity compared to 2A2 against RORl ⁺ epithelial tumor cells

RORl has been detected on many epithelial tumors, although it is unknown whether RORl expression is sufficient for recognition by RORl chimeric receptor T-cells. Using flow cytometry, we confirmed RORl expression on breast cancer lines MDA-MB-231 and 468, and on the renal cell carcinoma lines FARP, TREP, and RWL (Figure 10A). We then analyzed tumor recognition by CD8 ⁺ T-cells transduced with the Rl 2 RORl chimeric receptors with the optimal short spacer and 4- IBB domain, and observed efficient recognition of MDA-MB-231, MDA-MB- 468, FARP, TREP and RWL (Figure 11 A). We analyzed cytokine secretion and proliferation of T-cells modified with the R12 and 2A2 RORl -chimeric receptors after co-culture ith MDA-MB-231 , and observed greater cytokine production and proliferation with the R12 RORl chimeric receptor (Figure 1 1 B, C). Similar to what we observed with RORl ' B cell malignancies, the superior activation of RI 2 RORl chimeric receptor T cells after stimulation with MDA-MB-231 was not associated with increased AICD (R12: 9.8% vs. 2A2: 1 0.9%).

Discussion

RORl has attracted interest as a potential target for cancer immunotherapy due to its expression on the surface of many B-lymphoid and epithelial cancers, including subsets of lung, colorectal and renal cell cancer. We previously showed that CLL and MCL were specifically recognized by T-cells modified to express a RORl -specific chimeric receptor (Hudecek M, et al. Blood. 2010;1 16(22):4532-41. Epub 2010/08/13). The design and function of RORl -chimeric receptors has been improved through modification of the extracellular spacer domain and deriving the chimeric receptor from a scFV of higher affinity, and demonstrate that T-cells modified with designed RORl chimeric receptors have in vivo activity against RORl ⁺ B-cell lymphoma and in vitro activity against a wide range of epithelial tumors.

We compared the function of T-cells modified with RORl chimeric receptors derived from the 2A2 mAb that contained either the original long IgG4-Fc 'Hinge-CH2-CH3' . spacer that we have shown enables high level cell surface expression, or truncated intermediate 'Hinge-CH3' and short 'Hinge-only' spacer variants. We preserved the 12 AA Hinge domain in our short spacer construct based on prior data that a flexible spacer was required for separating the scFV from the T- cell membrane and allowing antigen recognition on tumor cells ( Fitzer-Attas CJ, et al., Harnessing Syk family tyrosine kinases as signaling domains for chimeric single chain of the variable domain receptors: optimal design for T cell activation. J

Immunol. 1998;160(l):145-54. Epub 1998/04/29.)

Our studies with the 2A2 RORl chimeric receptor show that T-cell cytokine secretion and proliferation after tumor cell recognition are superior with the intermediate and short spacer constructs compared to the long spacer construct. Staining with anti-F(ab) Abs showed equivalent chimeric receptor expression of all three receptors, demonstrating the improved T-cell function with the short spacer chimeric receptor was not due to differences in chimeric receptor density. This data supports the principle that the design of extracellular spacers should be tailored for each target molecule and epitope.

The affinity of the scFV selected for designing a chimeric receptor is an additional parameter that could affect T-cell recognition. We generated and characterized a panel of RORl -specific mAbs of different affinities and selected the R l 2 mAb, which recognizes an epitope in the Ig-like/Frizzled region as 2A2. R32 has a higher affinity for ROR l -protein due to a much slower dissociation. The R12 chimeric receptor, like the 2A2 chimeric receptor conferred optimal T-cel l recogn ition and function when designed with a short extracellular spacer. A direct comparison of proliferation and cytokine production after tumor engagement by T- cells modified with the 2A2 and R12 chimeric receptors demonstrated that the R12 chimeric receptor derived from the higher affinity roAb was superior. We were concerned that the slower dissociation of Rl 2 from RORl could prolong T-cell activation and confer an increased susceptibility to AICD. However, we detected a lower rate of AICD in T-cells modified with the R12 RORl -chimeric receptor compared to 2A2, demonstrating that the increased affinity of R12 had no detrimental effect on T-cell survival in our preclinical models.

ROR l has a potential advantage over CD 19 as a target for CLL and MCL since it is not expressed on normal mature naive and memory B-cells. However, there is a lower number of RORl molecules on B-cell tumors compared with CD19 and it is uncertain if an optimized RORl chimeric receptor would be as effective as a CD19 chimeric receptor similar in design to those being used in the clinic.

Unfortunately, B-cell tumor xenograft models used previously in NSG mice to evaluate the function of CD19 chimeric receptor T-cells including Raji, Daudi and Nalm-6, are not derived from CLL or MCL and do not constitutively express RORl . Thus, to compare targeting CD19 and RORl in vivo, we used the JeKo-1 MCL cell line, which naturally expresses both CD19 and RORl and engrafts in NSG mice. To make our model clinically relevant, we inoculated JeKo- 1 lymphoma cells intravenously to generate systemic tumors, and treated mice with T-cell products of uniform consistency once tumors were established. We found that T-cells expressing the high affinity R12 chimeric receptor conferred equivalent anti-tumor activity in vivo as CD19 chimeric receptor T-cells. Consistent with our in vitro analysis, the R12 RORl chimeric receptor also mediated superior activity in vivo compared to the optimal 2A2 RORl-chimeric receptor. These results should be interpreted cautiously since murine tumor models may not predict the efficacy of adoptive therapy in clinical settings. However, the results suggest that RORl warrants consideration as an alternative to CD19, or to provide an additional target to minimize the potential for CD19 loss variants to emerge.

ROR l appears to play a decisive role in survival of some epithelial tumors.

Thus, an advantage of targeting ROR l is that a single chimeric receptor may be useful to treat patients with a large number of hematopoietic and non-hematopoietic tumors. Our data shows for the first time that T-cells that express a designed ROR1 chimeric receptor efficiently recognize epithelial cancers in vitro. Cytokine secretion and T-cell proliferation induced by RORl ⁺ breast cancer cells were higher than that induced by leukemia cells, despite the absence of the CD80/86 costirnulatory ligand. The studies reported here demonstrate that the design of the extracellular spacer domain and chimeric receptor affinity are parameters that can be modulated to enhance the recognition of RORl ⁺ hematologic and epithelial tumors in vitro and in vivo by ROR1 -chimeric receptor modified T-ceils. The development of ROR1- chimeric receptors with enhanced tumor reactivity provides the opportunity for clinical applications in a variety of human cancers.

Example 2

Effect of extracellular spacer domain length on triggering of tumor cell lysis with a Her2-specific chimeric receptor that recognizes an epitope located proximal to the tumor cell membrane.

The effect of CAR spacer length on recognition and triggering of tumor cell recognition by CD8+ human T lymphocytes that expressed a HER2-specific chimeric receptor was examined using similar methods to those described above for ROR1. HER2-specific chimeric receptors were constructed using VL and VH chain segments of a HER2-specific mAb that recognized a membrane proximal epitope on HER2 (Figure 12A), and the scFVs were linked to fgG4 hinge/CH2/CH3, IgG4 hinge/CH3, and IgG4 hinge only extracellular spacer domains and to the CD28 transmembrane domain, 4-1BB and CD3 zeta signaling domains (Figure 12B). Primary CD8+ T cells were transduced with each of the HER2 chimeric receptors and selected for expression of the EGFR transducton marker (Figure 12D).

Expression of the HER 2 chimeric receptors and the size of each receptor was confirmed by Western Blot (Figure 12C). The T cells were then expanded with anti CD3 mAb and feeder cells and examined for their ability to recognize HER2+ tumor cells. As observed with the Rl 1 ROR 1 specific chimeric receptor, the HER2 chimeric receptor that contained a long extracellular spacer domain conferred superior T cell recognition of HER2+ tumor cells (Figure 12E).

Discussion

This example of the effect of extracellular spacer length on chimeric receptor modified T cell recognition of tumor cells used a chimeric receptor comprising a scFv built from the VH+L sequences of the Herceptin chimeric mAb. Studies by Cho et al (Nature 421 :756, 2003) localized to epitope location of Herceptin to a membrane proximal location on the HER2 (ERRB2) extracellular domain (Figure 12A). Based on our understanding of the structure of human TgG4 hinge:Fc variants (Figure 12B), we hypothesize that a membrane proximal location of the targeting epitope on an extracellular tumor cell antigen would best recognized by effector T cells that express a chimeric receptor encoding a long spacer. Our data demonstrating a gradient of cytolytic activity from near back ground activity by T cells expressing a short spacer Herceptin chimeric receptor, to intermediate activity by T cells expressing a medium length spacer chimeric receptor, and maximal lysis by T cells that expressed the long spacer chimeric receptor. Thus, the extracellular spacer has definitive effects on tumor recognition by T cells, and this data provides further support for the need to tailor chimeric receptor design based on epitope location of tumor expressed target molecules.

Example 3 -

Customizing spacer length and sequence for optimal recognition and in vivo efficacy of CD19 with chimeric receptor modified T cells.

Materials and Methods

Human subjects

Blood samples were obtained from healthy donors who provided written informed consent to participate in research protocols approved by the Institutional Review Board of the Fred Hutchinson Cancer Research Center (FHCRC). Pei'ipheral blood mononuclear cells (PBMC) were isolated by centrifugation over Ficoll- Hypaque (Sigma, St.Louis, MO), and cryopreserved in RPMI, 20% human serum and 10% dimethyl sulfoxide.

Cell lines

The K562, Raji, JeKo-1 , and 293T cel i lines were obtained from the American Type Culture Collection (Manassas, VA) and cultured as directed. A lentivirus encoding the ffliic-gene upstream of a T2A sequence and eGFP was produced in 293T cells and used to transduce Raj i and JeKo-1 tumor cells. Raji, and JeKo-1 cells were expanded after ientiviral transduction and the eGFP positive subset sort-purified.

Immunophenotyping PBMC and T-cell lines were stained with one or more of the following conj ugated monoclonal antibodies: CD3, CD4, CDS, CD25, CD45 A, CD45 O, CD62L, CD69 and matched isotype controls (BD Biosciences). Staining with propidium iodide (PI, BD Biosciences) was performed for live/dead cell discrimination as directed by the manufacturer. Flow analyses were done on a FACSCanto, sort-purifications on a FACSAriall (Becton Dickinson) and data analyzed using Flow Jo software (Treestar).

Vector construction and preparation of CD19 chimeric receptor encoding lentivirus

CD19 specific chimeric receptors were constructed using: (1 ) the VL and VH chain segments of the CD19-specific mAb FMC63 (SEQ ID NO:3), linked by a (G ₄ S) ₃ linker (SEQ ID NO:12)peptide (VL-linker-VH); (2) a spacer domain derived from IgG4-Fc (Uniprot Database: P01861, (SEQ ID NO: 13)) comprising either the Hinge-CH2- CH3 portion (229 AA, (SEQ ID NO:)) or Hinge only (12 AA; (SEQ ID NO:4)). Both spacers contained a S→ P substitution within the Hinge domain located at position 108 of the native IgG4-Fc protein; the 27 AA transmembrane domain of human CD28 (Uniprot Database: P10747, (SEQ ID NO: 14)); (4) a signaling module comprising either (i) the 41 AA cytoplasmic domain of human CD28 with an LL→ GG substitution located at position 186-187 of the native CD28 protein (SEQ ID NO: 14) ; and/or (ii) the 42 AA cytoplasmic domain of human 4- 1 BB (Uniprot Database: Q0701 1 , (SEQ TD NO: 15)); linked to (iii) the 1 12 AA cytoplasmic domain of isoform 3 of human CD3C, (Uniprot Database: P20963, (SEQ ID NO:16)); the self cleaving T2A sequence (SEQ ID NO: 8); and (6) a truncated epidermal growth factor receptor (EGFR)sequence (SEQ ID NO:9).

Codon-optimized nucleotide sequences encoding each trans gene were synthesized (Li eTechnologies, Carlsbad, CA) and cloned into the epHIV7 lentivirai vector using Nhel and Notl restriction sites. The epHIV7 lentivirai vector had been derived from the pHlV7 vector by replacing the cytomegalovirus promoter of pHiV7 with an EF- 1 promoter.

CD 19 chimeric receptor or tEGFR-encoding lentivirus was produced in 293T cells co-transfected with tiie lentivirai vector and the packaging vectors pCHGP-2, pCMV-Rev2 and pCMV-G using Calphos transfection reagent (C!ontech). Medium was changed 16 h after transfection, and lentivirus collected after 24, 48 and 72 h. Generation of T -cell lines expressing the CD19 chimeric receptors

Sort-purified CD8 ⁺ CD45RA ^" CD45RO ⁺ CD62L ⁺ central memory T -cells (TCM) of normal donors were activated with anti-CD3/ CD28 beads (Life

Technologies) according to the manufacturer's instructions, and transduced with lentiviral supernatant (MOl = 3) supplemented with 1 pg/mL polybrene (Millipore) on day 3 after activation by centrifugation at 2,100 rpm for 45 min at 32°C. T cells were expanded in RPM1, ! 0% human serum, 2 mM L-glutamine and 1 % penicillin- streptomycin (CTL medium), supplemented with recombinant human (rh) 1L-2 to a final concentration of 50 U/mL every 48 h. After expansion, an aliquot of each transduced T cell line was stained with biotin-conjugated anti-EGFR (epithelial growth factor receptor) antibody and streptavidin-beads (Miltenyi), and 1EGFR+ T cells isolated by immunomagnetic selection.

The tEGFR+ T-cell subset was then stimulated with irradiated (8,000 rad) TM EBV-LCL at a T cell: LCL ratio of 1 :7, and expanded for 8 days in CTL medium with addition of 50 U/mL rh IL-2 every 48 h.

Chromium release, cytokine secretion and CFSE proliferation assays

Target cells were labeled with ⁵¹ Cr (PerkinElmer) overnight, washed and incubated in triplicate at l ~2x 10 cells/well with effector T cells at various effector to target (E:T) ratios. Supernatants were harvested for γ counting after a 4-hour incubation and specific lysis calculated using the standard formula. For analyses of cytokine secretion, target and effector cells were plated in triplicate wells at an E:T ratio of 2: 1 (Raji) or 4: 1 (K562/CDI9 and K562), and INF-γ, TNF-a, IL-2, 1L-4, IL-6 and lL-10 measured by multiplex cytokine immunoassay (Luminex) in supernatant removed after a 24-hour incubation.

For analysis of proliferation, T cells were labeled with 0.2 μΜ

carboxyfluorescein succinimidyl ester (CFSE, Invitrogen), washed and plated in triplicate wells with stimulator cells at a ratio of 2: 1 (Raji) or 4: 1 (K562/CD19 and K562) in CTL medium without exogenous cytokines. After 72 h of incubation, cells were labeled with anti-CD3 mAb and propidium iodide (PI) to exclude dead cells from analysis. Samples were analyzed by flow cytometry and cell division of live CD3+ T-cells assessed by CFSE dilution.

Experiments in NOD/SOD and NOD/SCID/yc^ (NSG) mice All mouse experiments were approved by the FRCRC institutional Animal Chimeric receptore and Use Committee. Six- to 8-week old female NOD.CBI7- Prkdc ^scid /J (NOD/SCID) and NOD.Cg-Prkdc ^sdd I12rg ^tmlwjl /SzJ (NSG) mice were obtained from the Jackson Laboratory or bred in-house (FRCRC. Mice were injected intravenously (i. v.) with 0.5 x 10 ⁶ Raji-ffluc tumor cells via tail vein injection, and received injections of chimeric receptor-modified T cells, control T cells, or PBS via tail vein injection as indicated.

For bioluminescence imaging, mice received intraperitoneal (i,p.) injections of freshly prepared luciferin substrate (Caliper Life Sciences, MA) resuspended in PBS (15 yig/g body weight) and were then anesthetized with isoflurane in an induction chamber. After induction of deep anesthesia, mice were imaged using an Xenogen WIS In Vivo Imaging System (Caliper Life Sciences, MA) at 30, 12 and 14 minutes post i.p. injection of luciferin at an acquisition time of 1 second to 1 minute in small binning mode to obtain unsaturated images. Luciferase activity was analyzed using Living Image Software (Caliper Life Sciences, MA) and the photon flux analyzed within regions of interest that encompassed the entire body of each individual mouse.

Statistical analyses

Statistical analyses were performed using Prism Software (GraphPad, CA). Student's t-test was performed as a two-sided test with a confidence interval of 95% and results considered significant with a p-value of p<0.05. Statistical analysis of survival were done by Log-rank testing and results considered significant with a p- value of p<0.05.

Results

Preparation of polyclonal CD8 ⁺ Tc _M -derived cell lines that express CD19 chimeric receptors with long and short extracellular spacers

We constructed individual lentiviral vectors encoding a panel of codon- optimized CD 19 chimeric receptor genes to examine the influence of extracellular spacer length on the in vitro function and in vivo antitumor activity of CD 19 chimeric receptor-modified T cells. Each chimeric receptor was comprised of a single chain variable fragment corresponding to the sequence of the CDI9-specific mAb FMC63 (scFv: VL-VI I), a spacer derived from IgG4-Fc including either the 'Hinge-CH2-CH3' domain (229 AA, long spacer) or the 'Hinge' domain only (12 AA, short spacer), and a signaling module of ΟΌ3ζ with membrane proximal CD28 or 4-1 BB costimulatory domains, either alone or in tandem (Figure 13A). The transgene cassette included a truncated EGFR (tEGFR) downstream from the chimeric receptor gene and separated by a cleavable T2A element, to serve as a transduction, selection and in vivo tracking marker for chimeric receptor-modified T cells.

We isolated a CD8+ CD45RO+ CD62L+ central memoiy T cell (T _CM ) cell population by cell sorting from the blood of nonnal donors for transduction and expansion, because of the superior ability of TC to persist in vivo after adoptive transfer. CD8 - T cells were stimulated with anti CD3/28 beads, transduced with each of the lentiviral vectors, and expanded in culture for 18 days before being used for in vitro and in vivo experiments. (Figure 13B) Similar transduction efficiencies were achieved with each of the lentiviral vectors (mean 25%) and transgene-positive T cells were enriched to uniform purity by immunornagnetic selection using a biotinylated anti-EGFR mAb and streptavidin beads. Following tEGFR-enrichment, each of the CD19 chimeric receptor T cell lines were expanded by a single stimulation with CD19+B-LCL, without apparent differences in in vitro growth kinetics between T cell lines expressing the various CD 19 chimeric receptor constructs. After expansion, the tEGFR marker was expressed at equivalent levels on >90% of the T cells transduced with each of the vectors (Figure 13C).

CD19 chimeric receptors with long and short extracellular spacer domain confer specific anti-tumor reactivity in vitro

We compared the effector function of TcM-derived T cell lines modified to express CD 19 chimeric receptors with CD28 and 4-1 BB costimulatory signaling moieties, and either a short ('short/CD28Vshort/4-lBB ^r ) or long ( ^, long/CD28'; 'loog/4-l BB') extracellular spacer domain respectively. T cells expressing each of the 4 CD19 chimeric receptor constructs conferred specific cytolytic activity against CD 19 ⁺ Raji and JeKo-1 lymphoma cells, and against K562 cells that had been stably transfected with CD 19, but not native CD 19 ^" K562 ceils (Figure 14A). Quantitative analyses of cytokine production in response to stimulation with K562/CD19 or Raji tumor cells by multiplex cytokine assay (Luminex) showed production of lFls!-γ, TNF-a, 1L-2, 1L-4, IL-6, and lL-10 by T cells expressing each of the CD 19 chimeric receptors (Figure 14B). T cells expressing CD 19 chimeric receptors with a CD28 costimulatory domain produced significantly higher levels of ΙΚ -γ, T F-α, IL-2 and IL-10 compared to the corresponding constructs with a 4- IBB costimulatory domain (Figure 14B, C). There was significantly higher IFN-y production and significantly less 1L-4 production by T cells expressing the CD 19 'long/CD28' chimeric receptor compared with those expressing the 'short/CD28' chimeric receptor. Amongst the CD 19 chimeric receptors with 4- IBB costimulatory signaling module, we detected significantly higher levels of IFN-γ, TNF -a, IL-2, IL-4, and IL-10 secretion in T cells expressing the construct with the short spacer domain (Figure 14B, C).

We used CFSE dye dilution to analyze proliferation of T cells modified with each of the CD 19 chimeric receptors after engagement of CD 19+ tumor cells. Specific and vigorous proliferation of each of the CD19 chimeric receptor T cell lines was observed 72 hours following stimulation with either K562/CD19 or Raji. The average number of cell divisions was higlier for CD19 chimeric receptor T cells with a CD28 costimulatory domain compared to those with 4-1BB, consistent with greater IL-2 production by T cells expressing a CD28 containing chimeric receptor (Figure 14B-D). We also analyzed the proportion of chimeric receptor T cells that underwent activation induced cell death after stimulation with K562/CD19 and Raji tumor cells at the end of the 72-hours by costaining the culture with CD3+ and PI. We detected a higlier frequency of CD3 ⁺ CD8 ⁺ PI ⁺ T cells in the CD 19 chimeric receptor cell line 'long/4-1 BB', but few PI+ cells were observed with the other CD 19 chimeric receptors, (Figure 14E).

This analysis of in vitro effector functions was consistent with prior studies that have compared CD28 and 4- IBB costimulatory domains, and did not reveal differences in T cell function that would suggest that a particular CD 19 chimeric receptor construct from this panel would lack anti-tumor efficacy in vivo.

T cells expressing CDI9 chimeric receptors with short extracellular spacer domains but not long extracellular spacer domains eradicate Raji tumors in immunodeflcient mouse models

We next evaluated the in vivo antitumor efficacy of T cells modified with each of the CD19 chimeric receptors in immunodeficient (NOD/SCTD) mice engrafted with firefly luciferase transfected Raji cells (Raji-ffluc), which enables sequential quantitative analyses of tumor burden and distribution using bioluminescence imaging. NOD/SCID mice inoculated with 0.5x10 ⁶ Raji-ffluc cells via tail vein injection developed disseminated lymphoma, which if untreated led to hind limb paralysis after approximately 3.5 weeks, necessitating euthanasia. Tumor bearing mice were treated with 2 doses of CD8+ Tc _M -derived T cells modified with each of the CD1 chimeric receptors or with a tEGFR control vector administered on day 2 and day 9 after tumor inoculation (Figure 15A).

Surprisingly, only T cells modified to express CD 19 chimeric receptors with short extracellular spacer domain ( ^, short/CD28' and 'short/4- IBB ¹ ) eradicated Raji tumors in this model, whereas mice treated with T cells expressing CDI 9 chimeric receptors with long spacer ('Iong/CD28' and 'long/4-lBB') developed systemic lymphoma and hind limb paralysis with nearly identical kinetics as untreated mice or mice treated with control tEGFR+ T cells (Figure 15B, C). The striking difference in antitumor activity between CD 19 chimeric receptors with short and long spacer domains was highly significant and reproducible in multiple experiments with chimeric receptor T cell lines generated from 3 different normal donors.

The NOD/SCID lymphoma model may be suboptimal for predicting antitumor activity in a clinical setting because of the short interval between tumor inoculation and T cell admi istration and the greater resistance to engraftment of human cells compared to more immunodeficient mouse strains such as

NOD/SClD yc ^" ' ^" (NSG). Thus, we evaluated antitumor activity of adoptive therapy in a more clinically relevant model in which Raji-ffluc lymphoma was established in NSG mice, and the CD19 chimeric receptor T cells were administered after 7 days when the tumor was readily detectable in the bone marrow by bioluminescence imaging (Figure 16A). We performed initial dose titration experiments to determine the minimal dose of T cells transduced with the CD19 'short/4-ΙΒΒ' chimeric receptor that was required for eradication of established Raji tumors. A single dose of 2.5x10 ⁶ T cells expressing CD19-chimeric receptor 'short/4-l BB' promoted complete regression of established Raji tumors and resulted in long-term tumor-free survival in 100% of mice (Figure 16B,C). At the 2.5x10 ⁶ dose level, the T-cells were easily detected in the peripheral blood of NSG mice for at least 3 weeks following adoptive transfer and tumor eradication. Thus, this model enabled comparative studies both of antitumor activity and persistence of T cells modified with each of the CD19-chimeric receptors in our panel (Figure 16D). We then treated cohorts of NSG mice that were engrafted with Raji lymphoma with PBS alone, with a single dose of 2.5x10 ⁶ T cells expressing each of the CD 19 chimeric receptors or with T cells modified with a tEGFR encoding control vector (Figure 17A). ia this model of established lymphoma, T cells expressing CD 19 chimeric receptors with a short extracellular spacer domain and either 4- IBB or CD28 costimuiatory domains ('short/CD28* and 'short/4- IBB') mediated complete tumor regression over 7-10 days and all mice survived tumor free for >56 days. By contrast, mice treated with T cells modified to express CD19 chimeric receptors with a long spacer domain ('long CD28' and 'long/4-lBB'J exhibited tumor progression and had to be sacrificed at a similar time as mice that had received control tEGFR T cells (Figure 17B, C). The lack of in vivo antitumor activity of the chimeric receptor constructs with long spacers was unexpected given the ability of T cells expressing these constructs to lyse tumor cells in vitro, and the enhanced IL-2 production and proliferation after engagement of T cells expressing the 'long/CD28 ¹ CD19 chimeric receptor compared to the 4-1BB constructs.

To provide insight into the basis for the lack of efficacy, we performed sequential flow cytometry on peripheral blood samples of mice at intervals after the T cell infusion. AH mice treated with T cells expressing the 'short/CD28' and 'short/4- IBB ¹ CD19 chimeric receptors had significantly higher levels of transferred T cells in the blood at all time points after adoptive transfer, compared to mice treated with T cells that expressed corresponding CD19 chimeric receptors with long extracellular spacer (p<0.01) (Figure 17D). We did not observe significant differences in T-cell persistence in the peripheral blood of mice that had received T cells expressing CD19 chimeric receptors with CD28 or 4-1 BB co-stimulatory domains and short spacer domains (Figure 17D).

The in vivo anti-tumor efficacy of CD19 chimeric receptors with long spacers is not improved by increasing T cell dose or providing an additional costimuiatory domain

The lack of in vivo anti-tumor efficacy and the lower level of persisting chimeric receptor T cells in mice treated with T cells modified with CD 19 chimeric receptors with long spacer domains suggested that efficacy might be improved by increasing the chimeric receptor T cell dose or by including both CD28 and 4- IBB domains into the chimeric receptor to augment costimuiatory signaling. To eval uate this possibility we modified CD8+ T _CM with 'long/CD28', 'short CD28', and 'long/CD28_ 4- IBB' CD 19 chimeric receptor vectors and confirmed that the long/CD28 4- IBB' CD19 chimeric receptor conferred specific lysis and cytokine production in vitro after recognition of CD19+ target cells (Figure 18A-C).

Consistent with previous studies of CD19 chimeric receptors, the level of cytokine production and proliferation in vitro in T cells expressing the CD28_ 4-1BB' CDI9 chimeric receptor was inferior compared to the identical construct with CD28 alone, and superior to T cells expressing the 'long 4-IBB' CD19 chimeric receptor (Figure 18B, C).

Groups of NSG mice with established Raji tumors were then treated with a high dose of T cells (10 xlO ⁶ ) T cells expressing the 'Iong/CD28' CD19 chimeric receptor, the 'long/CD28_ 4-IBB' CDI9 chimeric receptor, the 'short/CD28' CD19- chimeric receptor, and tEGFR alone. Tumor burden was measured by

bioluminescence imaging and serial flow cytometric analyses of peripheral blood samples performed to determine the frequency of transferred T cells. Consistent with the results of our prior experiments using much lower doses of T cells, Raji tumors were completely eradicated in mice treated with T cells expressing the 'short/CD28' CD19-chimeric receptor. However, even with a 4-foid higher T cell dose, treatment with T cells expressing the 'long CD28' CD 19 chimeric receptor or the 'long CD28_ 4-1 BB' CD19 chimeric receptor did not provide a discernible antitumor effect (Figure 18D,E).

Thus, increasing the chimeric receptor T cell dose and adding a 4-1 BB costimnlatory domain to CD19 chimeric receptors failed to overcome the negative impact of the longer spacer domain on antitumor activity in vivo. Thus, in this model, anti-tumor reactivity of CD19 chimeric receptors is dictated to a great extent by the length of the extracellular spacer domain, and not by the intracellular costimulatory signaling modules.

T cells modified with CD 19 chimeric receptors that possess long extracellular spacers undergo activation induced cell death in vivo

We sought to determine potential mechanisms underlying the inferior in vivo antitumor activity of T cells that express CD19 chimeric receptors with long spacer domains. Because lower numbers of transfe red T cells modified to express CD19 chimeric receptors with long spacer domains were present in the blood, we considered the possibility that the T cells were not efficiently activated by tumor cells in vivo or conversely, that they underwent activation induced T cell death in vivo. Therefore, we labeled CD 19 chimeric receptor modified and corresponding control T cells with CFSE and administered these T cells to tumor bearing NSG/Raji mice to examine activation, proliferation and survival of T cells modified with each of the CDl 9 chimeric receptor constructs at tumor sites in vivo (Figure 19A). At the end of their in vitro expansion and immediately prior to CFSE labeling and infusion into NSG mice bearing established Raji tumors, T cells transduced with each of the CDl 9 chimeric receptors expressed low levels of the activation markers CD69 and CD25 (Figure 19B).

Bone marrow was obtained from subgroups of mice 24 and 72 hours after the T cell infusion to examine the frequency, activation and proliferation of transferred T cells. At 24 hours, tumor cells (CD45+ CD3-) were present in the bone marrow in all treatment groups and a large fraction of chimeric receptor T cells, but not control T cells, had upregulated CD69 and CD25. There was no measurable dilution of CFSE in the transferred chimeric receptor T cells. (Figure 19C) Both CD69 and CD25 were expressed in a higher proportion of T cells modified with 'long spacer' CD19 chimeric receptors, suggesting these cells may have received a stronger stimulus compared to T cells with 'short spacer ¹ CD19 chimeric receptors (Figure C). Despite evidence of T cell activation at 24 hours there were significantly lower numbers of chimeric receptor T cells in the bone marrow of mice treated with T cells modified with the CD28 and 4-1 BB 'long spacer' constructs compared to those modified with the CD28 and 4-1BB 'short spacer ¹ constructs, or with the control tEGFR vector (Figure 19C, E).

At 72 hours after T cell transfer, T cells expressing the 'short CD28' and

'short/4-lBB' CD 19 chimeric receptors had increased 3 to > 1 0 fold in frequency in the bone marrow and spleen, and had undergone several cell divisions (Figure 19D,E). Control tEGFR+ T cells remained present in the bone marrow and spleen at 72 hours at a level similar to that observed at 24 hours, and had not divided as measured by CFSE dilution. By contrast, the numbers of T cells expressing the

'long/CD28' and 'long/4-ΪΒΒ ¹ CDl 9 chimeric receptors had not increased in the bone marrow and spleen. (Figure 19D, E) Consistent with lower cell numbers, analysis of CFSE staining in viable PI- 'long/CD28' and 'long/4-IBB' CD19 chimeric receptor T cells demonstrated these cells had undergone a much lower number of cell divisions compared with 'short/CD28' and 'short/4-TBB' CD19 chimeric receptor T cells.

(Figure 19D)When the flow data was analyzed to include PI+ T cells, we detected a much higher frequency of P1+ CD3+ T cells in bone marrow and spleen of mice that received CD 19 chimeric receptor T cells with 'long spacer' domains, demonstrating that a significant proportion of T cells, despite being activated by tumor in vivo had undergone cell death (Figure 19F). Consistent with the bioluminescence imaging, CD45+ CD3- Raji tumor cells were present in greater numbers in the bone marrow of mice treated with T cells expressing CD19 chimeric receptors with long spacer domains or expressing tEGFR only compared to mice treated with CD19 chimeric receptors with short spacer domains (Figure 19D,E, G).

Collectively, the data provides evidence that CD19 chimeric receptors with long extracellular spacer domain, despite mediating equivalent or superior effector function in vitro and recognizing tumor in vivo, induce a high level of activation induced cell death in vivo and fail to eradicate established lymphoma.

Discussion

Chimeric receptors are artificial receptors that include an extracellular antigen-binding scFv, a spacer domain that provides separation of the scFv from the cell membrane and an intracellular signaling module that mediates T cell activation. Chimeric receptors that contain a scFv derived from the CD19-specific FMC63 mAb studied here, have advanced to testing in clinical trials in patients with B-cell malignancies. Antitumor activity and T cell persistence have varied substantially in different trials. Each of these clinical trials differed in potentially critical variables, including different gene transfer vectors, cell culture methodologies, and conditioning regimens prior to CD19 chimeric receptor T cell transfer.

We examined the possibility that the extracellular spacer domain of CD19 chimeric receptors may be an important determinant of anti-tumor activity in vivo, independent of the costimulatory signaling provided by the chimeric receptor. We derived spacer domains from lgG4-Fc, which enables high levels of chimeric receptor cell surface expression and is less likely to provoke recognition by innate immune cells compared to other IgG isotypes. We used the lgG4 'Hinge-CH2-CH3' in the design of the long (229 AA) spacer constructs and the lgG4 'Hinge' domain in our short (12 AA) spacer chimeric receptors. To compare the individual chimeric receptor constructs, we used purified (>90%) chimeric receptor positive CD8 TCM- derived T cells to remove differences in the cellular composition and transduction frequency as a potential source of bias in the analysis of in vitro and in vivo function. CD8 ⁺ TCM have been shown to have superior traits for adoptive immunotherapy, compared with other more prevalent T cell subsets in blood that persist poorly and are ineffective in tumor therapy. The CD 19 chimeric receptor T cells were generated using a standardized culture protocol that is similar to that used to derive chimeric receptor T cells for clinical trials. Our data show that CD 19 chimeric receptors with a short TgG4 'Hinge' spacer conferred potent anti-tumor reactivity in vitro and in vivo, whereas corresponding CD 19 chimeric receptors with a long spacer of TgG4 'Hinge-CH2-CH3', despite equivalent or superior reactivity in vitro, failed to confer significant anti-tumor effects in murine lymphoma models. Surprisingly, the length of the spacer domain proved to be a decisive element for in vivo antitumor activity, and the lack of efficacy of the 'long spacer' chimeric receptor could not be overcome by increasing the T cell dose.

We also observed major differences in cytokine secretion and proliferation in vitro between T cells expressing CD19 chimeric receptors containing CD28 and 4- 1BB costimulatory domains, with CD28 augmenting secretion of IFN-y, IL-2, and TNF-ct compared with 4-1BB. CD19 chimeric receptors that possessed a tandem CD28_4-1BB also produced higher levels of these cytokines compared to chimeric receptors encoding 4- IBB only. However, our data shows that these differences in in vitro function were not predictive of in vivo anti-tumor efficacy, since CD1 chimeric receptors with either CD28 or 4-1 BB costimulatory domain and a short spacer were similarly effective at eradicating advanced established Raji tumors in NSG mice. In contrast, CD19 chimeric receptors with suboptimal spacer length and CD28, 4-1BB, or both costimulatory domains, despite conferring similar in vitro function as the identical chimeric receptor construct with a short spacer domain, lacked significant anti-tumor activity in vivo, demonstrating the contribution of spacer length to in vivo function of chimeric receptor T cells.

Our studies provide insight into the mechanism responsible for the lack of in vivo efficacy of CD19 chimeric receptors with long spacer domains. T cells expressing CD19 chimeric receptors with both long and short spacer domains could be detected in the bone marrow and spleen after adoptive transfer into N SG mice bearing established Raji lymphoma, and the majority were activated as demonstrated by upregulation of CD25 and CD69. However, T cells modified to express a CD19 chimeric receptor with a long spacer domain exhibited a steep decline in cell number, in contrast to the marked in vivo expansion of T cells expressing CD19 chimeric receptors with a short spacer domain. The decline in T cell number was a consequence of much higher levels of cell death in the first 72 hours after adoptive transfer compared with T cells with short spacer domains, and control T cells that did not express a CD 19 chimeric receptor. Collectively, these data indicate that recognition of tumor cells in vivo resulted in death of T cells expressing CD19- chimeric receptors with long spacer domains. A similar mechanism may explain the short duration and low levels of T cell persistence in the clinical trials that employed long spacer CD19-chimeric receptors (14).

The studies reported here are the first to show that the spacer domains of CD 19 chimeric receptors that lack intrinsic signaling properties have dramatic effects on in vivo antitumor activity independent of costimulatory signaling, and identify the importance of analyzing the optimal composition of tills region in the design of chimeric receptors for clinical applications.

The foregoing is illustrative of the present invention, and is not to be construed as limiting thereof. The invention is defined by the following claims, with equivalents of the claims to be included therein. All references and documents referred to herein are hereby incorporated by reference.

Table 1

Sequence of anti-CD19 short spacer chimeric receptor

GMCSFRss-CD19scFv-IgG4hinge-CD28tni-41BB-Zeta-T2A-EGFRt

v4/gctgctgctggtgaccagcctgctgctgtgcgagctgccccaccccgcctttct gctgatcccc

(GMCSFRss) (SEQ ID NO:2)

Gacatccagatgacccagaccacctccagcctgagcgccagcctgggcgaccgggtgacc atcagctgccggg ccagccaggacatcagcaagtacctgaactggtatcagcagaagcccgacggcaccgtca agctgctgatctac cacaccagccggctgcacagcggcgtgcccagccggtttagcggcagcggctccggcacc gactacagcctgac catctccaacctggaacaggaagatatcgccacctacttttgccagcagggcaacacact gccctacacctttggc ggcggaacaaagctggaaatcaccggcagcacctccggcagcggcaagcctggcagcggc gagggcagcacc aagggcgaggtgaagctgcaggaaagcggccctggcctggtggcccccagccagagcctg agcgtgacctgca ccgtgagcggcgtgagcctgcccgactacggcgtgagctggatccggcagccccccagga agggcctggaatg gctgggcgtgatctggggcagcgagaccacctactacaacagcgccctgaagagccggct gaccatcatcaag gacaacagcaagagccaggtgttcctgaagatgaacagcctgcagaccgacgacaccgcc atctactactgcgc caagcactactactacggcggcagctacgccatggactactggggccagggcaccagcgt gaccgtgagcagc

(CD19scFv) (SEQ ID NO:3)

Gaatctaagtacggaccgccctgccccccttgccct (IgG4hinge) (SEQ ID NO:4)

Atgttctgggtgctggtggtggtcggaggcgtgctggcctgctacagcctgctggtcacc gtggccttcatcatctt ttgggtg (CD28tm-)(SEQ TD NO:5)

Aaacggggcagaaagaaactcctgtatatattcaaacaaccatttatgagaccagtacaa actactcaagagg aagatggctgtagctgccgatttccagaagaagaagaaggaggatgtgaactg (41BB) (SEQ ID NO:6)

Cgggtgaagttcagcagaagcgccgacgcccctgcctaccagcagggccagaatcagctg tacaacgagctga acctgggcagaagggaagagtacgacgtcctggataagcggagaggccgggaccctgaga tgggcggcaagc ctcggcggaagaacccccaggaaggcctgtataacgaactgcagaaagacaagatggccg aggcctacagcg agatcggcatgaagggcgagcggaggcggggcaagggccacgacggcctgtatcagggcc tgtccaccgcca ccaaggatacctacgacgccctgcacatgcaggccctgcccccaagg (CD3Zeta)- (SEQ ID NO:7)

Ctcgagggcggcggagagggcagaggaagtcttctaacatgcggtgacgtggaggag aatcccggccctagg (T2A) (SEQ ID NO:9)

Atgcttctcctggtgacaagccttctgctctgtgagttaccacacccagcattcctcctg atcccacgcaaagtgtg taacggaatagg tattggtga atttaa agactca ctctccataaatgc tac gaatattaaa cacttcaaaaactgc acctccatcagtggcgatctccacatccigccggtggcatttaggggtgactccttcaca catactcctcctctggat ccacaggaactggatattctgaaaaccgtaaaggaaatcacagggtttttgctgattcag gcttggcctgaaaac aggacggacctccatgcctttgagaacctagaaatcatacgcggcaggaccaagcaacat ggtcagttttctctt gcagtcgtcagcctgaacataacatccttgggattacgctccctcaaggagataagtgat ggagatgtgataattt caggaaacaaaaatttgtgctatgcaaatacaataaactggaaaaaactgtttgggacct ccggtcagaaaacc aaa attata agcaacagaggtgaaaacagctgcaa ggccaca ggcc aggtctgccatgcc ttgtgctcccccga gggctgctggggcccggagcccagggactgcgtctcttgccggaatgtcagccgaggcag ggaatgcgtggac aagtgcaaccttctggagggtgagccaagggagtttgtggagaactctgagtgcatacag tgccacccagagtg cctgcctcaggccatgaacatcacctgcacaggacggggaccagacaactgtatccagtg tgcccactacattga cggcccccactgcgtcaagacctgcccggcaggagtcatgggagaaaacaacaccctggt ciggaagtacgca gacgccggccatgtgtgccacctgtgccatccaaactgcacctacggatgcactgggcca ggtcttgaaggctgt ccaacgaatgggcctaagatcccgtccatcgccactgggatggtgggggccctcctcttg ctgctggtggtggccc tggggatcggcctcttcatgfga (EGFRf) (SEQ ID NO:9)

Table 2

GMCSFRss

DNA: ArGCTGCTGCTGGTGACCAGCCTGCTGCTGTGCGAGCTGCCCCACCCCGCC

AA: M L L L V T S L L L C E L P H P A

CD19scFv

DN : TTTCTGCTGATCCCC : GACATCCAGATGACCCAGACCACCTCCAGCCTGAGC

AA: F L L I P D I Q M T Q T T S S L S DNA: GCGAGCCTGGGCGACCGGGTGACCATCAGCTGCCGGGCCAGCCAGGACATC

AA: A S L G D R V T I S C R A S Q D I

DNA : AGCAAGTACCTGAACTGGTATCAGCAGAAGCCCGACGGCACCGTCAAGCTG

AA: S K Y L N W Y Q Q K P D G T V K L

DNA: CTGATCTACCACACCAGCCGGCTGCACAGCGGCGTGCCCAGCCGGTTTAGC

AA: L I Y H T S R L H S G V P S R F S

DNA: GGCAGCGGCTCCGGCACCGACTACAGCGTGACCATCTCCAACCTGGAACAG AA: G S G S G T D Y S L T I S N L E Q

DNA : GAAGATATCGCCACCTACTTTTGCCAGCAGGGCAACACACTGCCCTACACC

AA: E D I A T Y F C Q Q G N T L P Y T DNA: TTTGGCGGCGGAACAAAGCTGGAAATCACCGGCAGCACCTCCGGCAGCGGC

AA: F G G G T K L E I T G S T S G S G

DNA: AAGCCTGGCAGCGGCGAGGGCAGCACCAAGGGCGAGGTGAAGCTGCAGGAA

AA: K P G S G E G S T K G E V K L Q E

DNA: AGCGGCCCTGGCCTGGTGGCCCCCAGCCAGAGCCTGAGCGTGACCTGCACC

AA: S G P G L V A P S Q S L S V T C T

DNA: GTGAGCGGCGTGAGCCTGCCCGACTACGGCGTGAGCTGGATCCGGCAGCCC AA: V S G V S L P D Y G V S W I R Q P

DNA: CCCAGGAAGGGCCTGGAATGGCTGGGCGTGATCTGGGGCAGCGAGACCACC

AA: P R K G L E W L G V I W G S E T T DNA: TACTACAACAGCGCCCTGAAGAGCCGGCTGACCATCATCAAGGACAACAGC

AA: Y Y N S A L K S R L T I I K D N S

DNA: AAGAGCCAGGTGTTCCTGAAGATGAACAGCCTGCAGACCGACGACACCGCC

AA: K S Q V F L K N S L Q T D D T A

DN : ATC T ACTACT GCGCCAAGCACTACTACTAC GGCGGCAGCT ACGC CATG GAC

AA: I Y Y C A K H Y Y Y G G S Y A M D

IgG4hinge DNA : TACTGGGGCCAGGGCACCAGCGTGACCGTGAGCAGC : GAGAGCAAGTACGGA

AA: Y W G Q G T S V T V S S E S K Y G

CD28tm

DNA : CCGCCCTGCCCCCCTTGCCC : ATGTTCTGGGTGCTGGTGGTGGTCGGAGGC AA: P P C P P C P M F W V L V V V G G

DNA : GTGCTGGCCTGCTACAGCCTGCTGGTCACCGTGGCCTTCATCATCTTTTGG AA: V L A C Y S L L V T V A F I I F W 41BB

DNA : GTG : AAACGGGGCAGAAAGAAACTCCTGTATATATTCAAACAACCATTTATG AA: V K R G R K K L L Y I F K Q P F M

DNA: AGACCAGTACAAACTACTCAAGAGGAAGATGGCTGTAGCTGCCGATTTCCA AA: R P V Q T T Q E E D G C S C R F P

CD3Zeia

DN : GAAGAAGAAGAAGGAGGATGTGAACTGCGGGTGAAG : TTCAGCAGAAGCGCC AA: E E E E G G C E L R V K F S R S A

DNA : GACGCCCCTGCCTACCAGCAGGGCCAGAATCAGCTGTACAACGAGCTGAAC AA: D A P A Y Q Q G Q N Q L Y N E L N

DNA: GTGGGCAGAAGGGAAGAGTACGACGTCCTGGATAAGCGGAGAGGCCGGGAC AA: L G R E E Y D V L D K R R G R D

DN : CCTGAGATGGGCGGCAAGCCTCGGCGGAAGAACCCCCAGGAAGGCCTGTAT AA: P E M G G K P R R K N P Q E G L Y

DNA: AACGAACTGCAGAAAGACAAGATGGCCGAGGCCTACAGCGAGATCGGCATG AA: N E L Q K D K M A E A Y S E I G M

DNA : AAGGGCGAGCGGAGGCGGGGCAAGGGCCACGACGGCCTGTATCAGGGCCTG AA: K G E R R R G G H D G L Y Q G L

DN : TCCAC CGCG ACCAAGGATACCTAC GACGCC CTGCACATGCAGG CCCTGCCC AA: S T A T K D T Y D A L H M Q A L P

T2A

DNA: CCAAGG : CTCGAGGGCGGCGGAGAGGGCAGAGGAAGTCTTCTAACATGCGGT AA: ^' P R L E G G G E G R G S L L T C G

EGFRt

DNA: GACGTGGAGGAGAATCCCGGCCCTAGG :ATGCTTCTCCTGGTGACAAGCCTT

AA: D V E E N P G P R M L L L V T S L

DNA : CTGCTCTGTGAGTTACCACACCCAGCATTCCTCCTGATCCCACGCAAAGTG

AA: L L C E L P H P A F L L I P R K V

DNA : TGTAACGGAATAGGTATTGGTGAATTTAAAGACTCACTCTCCATAAATGCT

AA: C N G I G I G E F K D S L S I N A

DN : ACGAATATTAAACACTTCAAAAACTGCACCTCGATCAGTGGCGATCTCCAC AA: T N I K H F K N C T S I S G D L H

DNA : ATCCTGCCGGTGGCATTTAGGGGTGACTCCTTCACACATACTCCTCCTCTG

AA: I L P V A F R G D S F T H T P P L DNA: GATCCACAGGAACTGGATATTCTGAAAACCGTAAAGGAAATCACAGGGTTT

AA: D P Q E L D I. L K T V K E I T G F

DNA: TTGCTGATTCAGGCTTGGCCTGAAAACAGGACGGACCTCCATGCCTTTGAG

Aft: L L I Q A W P E N R T D- L H A F E

DNA: AACCTAGAAATCATACGGGGCAGGACCAAGCAACATGGTCAGTTTTCTCTT

AA: N L E I I R G R T K Q H G Q F S L

DNA: GGAGTCGTCAGCCTGAACATAACATCCTTGGGATTACGCTCCCTCAAGGAG

AA: A V V S L N I T S L G L R S L K E

DNA: ATAAGTGATGGAGATGTGATAATTTCAGGAAACAAAAATTTGTGCTATGCA

AA: I S D G D V I I S G N K N L C Y A

DNA: AATACAATAAACTGGAAAAAACTGTTTGGGACCTCCGGTCAGAAAACCAAA

AA: N T I N W K K L F G T S G Q K T K

DNA: ATTATAAGCAACAGAGGTGAAAACAGCTGCAAGGCCACAGGCCAGGTCTGC

AA: I I S N R G E N S C K A T G Q V C

DNA: CATGCCTTGTGCTCCCCCGAGGGCTGCTGGGGCCCGGAGCCCAGGGACTGC

AA: H A L C S P E G C W G P E P R D C

DNA: GTCTCTTGCCGGAATGTCAGCCGAGGCAGGGAATGCGTGGACAAGTGCAAC

AA: V S C R N V S H- G R E C V D K C N

DNA: CTTCTGGAGGGTGAGCCAAGGGAGTTTGTGGAGAACTCTGAGTGCATACAG

AA: L L E G E P R E F V E N S E C I Q

DN : TGCCACCCAGAGTGCCTGCCTCAGGCCATGAACATCACCTGCACAGGACGG

AA: C H P E C L P Q A M N I T C T G R

DNA: GGACCAGACAACTGTATCCAGTGTGCCCACTACATTGACGGCCCCCACTGC

AA: G P D N C I Q C A H Y I D G P H C

DNA: GTCAAGACCTGCCCGGCAGGAGTCATGGGAGAAAACAACACCCTGGTCTGG

AA: V K T C P A G V M G E N N T L V W

DN : AAGTACGCAGACGCCGGCCATGTGTGCCACCTGTGCCATCCAAACTGCACC

AA: K Y A D A G H V C H L C H P N C T

DNA: TACGGATGCACTGGGCCAGGTCTTGAAGGGTGTCCAACGAATGGGCCTAAG

AA: Y G C T G P G L E G C P T N G P

DN : ATCCCGTCCATCGCCACTGGGATGGTGGGGGCCCTCCTCTTGCTGCTGGTG

AA: I P S I A T G M V G A L L L L L V

DNA: GTGGCCCTGGGGATCGGCCTCTTCATG!TGA (SEQ ID NO: 10) AA: V A L G I G L F M * (SEQ ID NO: li) Table 3

ZXR-014 Nucleotide and amino acid sequences (map of sections)

GMCSFRss: nt2084-2149

CD19scFv: nt2150-2884

Igg4Hinge: nt2885-2920

CD28tm: nt2921-3004

41BB: nt3005-3130

Zeta: nt3131-3466

T2A: nt3467-3538

EGFRt: nt3539-4612

Primers for sequencing:

Oligo name Sequence Region

OJ02649 ATCAAAAGAATAGACCGAGATAGGGT pre-U5(SEQ ID MO:22) OJ02648 CCGTACCTTTAAGACCAATGACTTAC delU3(SEQ ID NO:23) OJ02650 TTGAGAGTTTTCGCCCCG mid-Ampr(SEQ ID NO:24) oJ02651 AATAGACAGATCGCTGAGATAGGT post-Ampr(SEQ ID NO:25)

OJ02652 CAGGTATCCGGTAAGCGG CoEl ori(SEQ ID NO:26)

OJ02653 CGACCAGCAACCATAGTCC SV40(SEQ ID NO:27)

OJ02654 TAGCGGTTTGACTCACGG CMV(SEQ ID NO:28)

OJ02655 GCAGGGAGCTAGAACGATTC psi(SEQ ID NO:29)

OJ02656 ATTGTCTGGTATAGTGCAGCAG RRE(SEQ ID NO:30)

OJ02657 TCGC A ACGGG TTTGCC EFlp(SEQ ID O:31) oJ02658 AGGAAGATATCGCCACCTACT CD19Rop(SEQ ID NO:32)

OJ02601 CGGGTGAAGTTCAGCAGAAG Zeta(SEQ ID NO:33)

OJ02735 ACTGTGTTTGCTG ACG CAAC WPRE(SEQ ID NO:34)

OJ02715 ATGCTTCTCCTG G TG ACAAG EGFRt(SEQ ID NO:35) Table 4 Uniprot P0861 IgG4-Fc(SEQ ID NO:13)

NVFSCSVMHE ALHNHYTQKS LSLSLGK

1-98 CHI

99-110 Hinge

111-220 CH2

221-327 CH3

Position 108 S→P

Table 5 Dni rot P10747 CD28(SEQ ID NO:14)

SKRSRLLHSD YMNMTPRRPG PTRKHYQPYA PPRDFAAYRS

1-18 signal peptide

19-152 extracellular domain

153-179 transmembrane domain

180-220 intracellular domain

Position 186-187 LL→GG

Table 6 Uni rot Q07011 4-lBB(SEQ ID NO: 15)

CSCR.FPEEEE GGCEL

1-23 signal peptide

24-186 extracellular domain

187-213 transmembrane domain

214-255 intracellular domain

Table 7 Oniprot P20963 human ΟΌ3ζ isoform 3 (SEQIDNO:16)

EAYSEIGMKG ERRRGKGHDG LYQGLSTATK DTYDALHMQA LPPR

1-21 signal peptide

22-30 extracellular

31-51 transmembrane

52-164 intracellular domain

61-89 ITAM1

100-128 ITAM2

131-159 ITAM3

Table 8 Exemplary Hinge region Sequences

Human IgGl EPKSCD THTCPPCP (SEQ ID NO: 17)

Human IgG2 ERKCCVECPPCP (SEQ TD NO: 18)

Human IgG3 ELKTPLGDTHTCPRCP (EPKSCDTPPPCPRCP) ₃ (SEQ ID NO:19)

Human IgG4 ESKYGPPCPSCP (SEQ ID NO:20)

Modified Human IgG4 ESKYGPPCPPCP (SEQ ID NO:2I)

Modified Human IgG4 YGPPCPPCP (SEQ ID NO:51)

Modified Human TgG4 KYGPPCPPCP (SEQ ID NO:52)

Modified Human IgG4 EWKYGPPCPPCP (SEQ ID NO:53)

Table 9

12 long spacer CAR: PJ R12-CH2-CH3- 1BB-Z-T2A EGFR (SEQ ID NO:37)

GTTAGACCAGATCTGAGCCTGGGAGCTCTCTGGCTAACTAGGGAACCCACTGCT TAAGCCTCAATAAAGCTTGCCTTGAGTGCTTCAAGTAGTGTGTGCCCGTCTGTTG TGTGACTCTGGTAACTAGAGATCCCTCAGACCCTTTTAGTCAGTGTGGAAAATC TCTAGCAGTGGCGCCCGAACAGGGACTTGAAAGCGAAAGGGAAACCAGAGGAG CTCTCTCGACGCAGGACTCGGCTTGCTGAAGCGCGCACGGCAAGAGGCGAGGG GCGGCGACTGGTGAGTACGCCAAAAATTTTGACTAGCGGAGGCTAGAAGGAGA GAGATGGGTGCGAGAGCGTCAGTATTAAGCGGGGGAGAATTAGATCGATGGGA AAAAATTCGGTTAAGGCCAGGGGGAAAGAAAAAATATAAATTAAAACATATAG TATGGGCAAGCAGGGAGCTAGAACGATTCGCAGTTAATCCTGGCCTGTTAGAAA CATCAGAAGGCTGTAGACAAATACTGGGACAGCTACAACCATCCCTTCAGACA GGATCAGAAGAACTTAGATCATTATATAATACAGTAGCAACCCTCTATTGTGTG CATCAAAGGATAGAGATAAAAGACACCAAGGAAGCTTTAGACAAGATAGAGGA AGAGCAAAACAAAAGTAAGAAAAAAGCACAGCAAGCAGCAGCTGACACAGGA CACAGCAATCAGGTCAGCCAAAATTACCCTATAGTGCAGAACATCGAGGGGCA AATGGTACATCAGGCCATATCACCTAGAACTTTAAATGCATGGGTAAAAGTAGT AGAAGAGAAGGCTTTCAGCCCAGAAGTGATACCCATGTTTTCAGCATTATCAGA AGGAGCCACCCCACAAGATTTAAACACCATGCTAAACACAGTGGGGGGACATC AAGCAGCCATGCAAATGTTAAAAGAGACCATCAATGAGGAAGCTGCAGGCAAA GAGAAGAGTGGTGCAGAGAGAAAAAAGAGCAGTGGGAATAGGAGCTTTGTTCC TTGGGTTCTTGGGAGCAGCAGGAAGCACTATGGGCGCAGCGTCAATGACGCTG ACGGTACAGGCCAGACAATTATTGTCTGGTATAGTGCAGCAGCAGAACAATTTG CTG AGGG CTATTG AGGCGCAAC AGC ATCTGTTG C AACTCACAGTCTGGGGC ATC AAGCAGCTCCAGGCAAGAATCCTGGCTGTGGAAAGATACCTAAAGGATCAACA GCTCCTGGGGATTTGGGGTTGCTCTGGAAAACTCATTTGCACCACTGCTGTGCCT TGGATCTACAAATGGCAGTATTCATCCACAATTTTAAAAGAAAAGGGGGGATTG GGGGGTACAGTGCAGGGGAAAGAATAGTAGACATAATAGCAACAGACATACAA ACTAAAGAATTACAAAAACAAATTACAAAAATTCAAAATTTTCGGGTTTATTAC AGGGACAGCAGAGATCCAGTTTGGGGATCAATTGCATGAAGAATCTGCTTAGG GTTAGGCGTTTTGCGCTGCTTCGCGAGGATCTGCGATCGCTCCGGTGCCCGTCA GTGGGCAGAGCGCACATCGCCCACAGTCCCCGAGAAGTTGGGGGGAGGGGTCG GCAATTGAACCGGTGCCTAGAGAAGGTGGCGCGGGGTAAACTGGGAAAGTGAT GTCGTGTACTGGCTCCGCCTTTTTCCCGAGGGTGGGGGAGAACCGTATATAAGT GCAGTAGTCGCCGTGAACGTTCTTTTTCGCAACGGGTTTGCCGCCAGAACACAG CTGAAGCTTGGAGGGGCTCGCATCTCTCCTTCACGCGCCCGCCGCCCTACCTGA GGCCGCCATCCACGCCGGTTGAGTCGCGTTCTGCCGCCTCCCGCCTGTGGTGCC TCCTGAACTGC GTCCG CCGTCTAG GTAAGTTT A A AGCTC AGGTCG AG ACCGGG C CTTTGTCCGGCGCTCCCTTGG AG CCTACCTAG ACTC AG CCG GCTCTCCACG CTTT GCCTGACCCTGCTTGCTCAACTCTACGTCTTTGTTTCGTTTTCTGTTCTGCGCCGT TACAGATCCAAGCTGTGACCGGCGCCTACG

[CiCTAGCjGAATTCCTCGAGGCC

ACC(ATGjCTGCTGCTGGTG AC AAGCCTGCTGCTGTGCG AG CTG CCCC ACCCCG CC TTTCTGCTG A TCCCCCAGG A ACAGCTCGTCG A A AGCGGCGGCAGACTGGTGACA CCTG GCGGC AGCCTG ACCCTG AGCTG CA AG G CC AGCGGCTTCG ACTTC AGCGCC TACTACATGAGCTGGGTCCGCCAGGCCCCTGGCAAGGGACTGGAATGGATCGCC ACCATCTACCCCAGCAGCGGCAAGACCTACTACGCCACCTGGGTGAACGGACG GTTCACCATCTCCAGCGACAACGCCCAGAACACCGTGGACCTGCAGATGAACA GCCTGACAGCCGCCGACCGGGCCACCTACTTTTGCGCCAGAGACAGCTACGCCG ACGACGGCGCCCTGTTCAACATCTGGGGCCCTGGCACCCTGGTGACAATCTCTA GCGGCGGAGGCGGATCTGGTGGCGGAGGAAGTGGCGGCGGAGGATCTGAGCTG GTGCTGACCCAGAGCCCCTCTGTGTCTGCTGCCCTGGGAAGCCCTGCCAAGATC ACCTGTACCCTGAGCAGCGCCCACAAGACCGACACCATCGACTGGTATCAGCA GCTGCAGGGCGAGGCCCCCAGATACCTGATGCAGGTGCAGAGCGACGGCAGCT ACACCAAGAGGCCAGGCGTGCCCGACCGGTTCAGCGGATCTAGCTCTGGCGCC GACCGCTACCTGATCATCCCCAGCGTGCAGGCCGATGACGAGGCCGATTACTAC TGTGGCGCCGACTACATCGGCGGCTACGTGTTCGGCGGAGGCACCCAGCTGACC GTGACCGGCGAGTCTAAG

IgG4 spacer

11 CGGACCG|CCCTGCCCCCCTTGCCCT

CH2

GCCCCCGAGTTCCTGGGCGGACCCAGCGTGTTCCTGTTCCCCCCCAAGCCCAAG GACACCCTGATGATCAGCCGGACCCCCGAGGTGACCTGCGTGGTGGTGGACGTG AGCCAGGAAGATCCCGAGGTCCAGTTCAATTGGTACGTGGACGGCGTGGAAGT GCACAACGCCAAGACCAAGCCCAGAGAGGAACAGTTCAACAGCACCTACCGGG TGGTGTCTGTGCTGACCGTGCTGCACCAGGACTGGCTGAACGGCAAAGAATACA AGTGCAAGGTGTCCAACAAGGGCCTGCCCAGCAGCATCGAAAAGACCATCAGC AAGGCCAAG CH3

GGCCAGCCTCGCGAGCCCCAGGTGTACACCCTGCCTCCCTCCCAGGAAGAGATG ACCAAGAACCAGGTGTCCCTGACCTGCCTGGTGAAGGGCTTCTACCCCAGCGAC ATCGCCGTGGAGTGGGAGAGCAACGGCCAGCCTGAGAACAACTACAAGACCAC CCCTCCCGTGCTGGACAGCGACGGCAGCTTCTTCCTGTACAGCCGGCTGACCGT GGACAAGAGCCGGTGGCAGGAAGGCAACGTCTTTAGCTGCAGCGTGATGCACG AG GCCCTGC ACAACC ACTAC ACCCAGAAG AGCCTG AGCCTGTCCCTGGGC AAG

4-1BB

ATGTTCTGGGTGCTGGTGGTGGTGGGCGGGGTGCTGGCCTGCTACAGCCTGCTG GTGACAGTGGCCTTCATCATCTTTTGGGTGAAACGGGGCAGAAAGAAACTCCTG TATATATTCAAACAACCATTTATGAGACCAGTACAAACTACTCAAGAGGAAGAT GGCTGTAGCTGCCGATTTCCAGAAGAAGAAGAAGGAGGATGTGAACTG CD3 zeta

CGGGTGAAGTTCAGCAGAAGCGCCGACGCCCCTGCCTACCAGCAGGGCCAGAA TCAG CTGTAC A ACG AGCTG A ACCTGGGC AG AAG G GAAG AGTACG ACGTCCTGG ATAAGCGGAGAGGCCGGGACCCTGAGATGGGCGGCAAGCCTCGGCGGAAGAAC CCCCAGGAAGGCCTGTATAACGAACTGCAGAAAGACAAGATGGCCGAGGCCTA CAGCGAGATCGGCATGAAGGGCGAGCGGAGGCGGGGCAAGGGCCACGACGGC CTGTATCAGGGCCTGTCCACCGCCACCAAGGATACCTACGACGCCCTGCACATG CAGGCCCTGCCCCCAAGG

T2A

|CTCGAG|GGCGGCGGAGAGGGCAGAGGAAGTCTTCTAACATGCGGTGACGTGGA

GGAGAATCCCGGCCCTAGG

tEGFR ATGCTTCTCCTGGTGACAAGCCTTCTGCTCTGTGAGTTACCACACCCAGCATTCC TCCTGATCCCACGCAAAGTGTGTAACGGAATAGGTATTGGTGAATTTAAAGACT CACTCTCCATAAATGCTACGAATATTAAACACTTCAAAAACTGCACCTCCATCA GTGGCGATCTCCACATCCTGCCGGTGGCATTTAGGGGTGACTCCTTCACACATA CTCCTCCTCT]GGATCC|ACAGGAACTGGATATTCTGAAAACCGTAAAGGAAATCA CAGGGTTTTTGCTGATTCAGGCTTGGCCTGAAAACAGGACGGACCTCCATGCCT TTGAGAACCTAGAAATCATACGCGGCAGGACCAAGCAACATGGTCAGTTTTCTC TTGCAGTCGTCAGCCTGAACATAACATCCTTGGGATTACGCTCCCTCAAGGAGA TAAGTGATGGAGATGTGATAATTTCAGGAAACAAAAATTTGTGCTATGCAAATA CAATAAACTGGAAAAAACTGTTTGGGACCTCCGGTCAGAAAACCAAAATTATA AGCAACAGAGGTGAAAACAGCTGCAAGGCCACAGGCCAGGTCTGCCATGCCTT GTGCTCCCCCGAGGGCTGCTGGGGCCCGGAGCCCAGGGACTGCGTCTCTTGCCG GAATGTCAGCCGAGGCAGGGAATGCGTGGACAAGTGCAACCTTCTGGAGGGTG AGCCAAGGGAGTTTGTGGAGAACTCTGAGTGCATACAGTGCCACCCAGAGTGC CTGCCTCAGGCCATGAACATCACCTGCACAGGACGGGGACCAGACAACTGTATC CAGTGTGCCCACTACATTGACGGCCCCCACTGCGTCAAGACCTGCCCGGCAGGA GTCATGGGAGAAAACAACACCCTGGTCTGGAAGTACGCAGACGCCGGCCATGT GTGCCACCTGTGCCATCCAAACTGCACCTACGGATGCACTGGGCCAGGTCTTGA AGGCTGTCCAACGAATGGGCCTAAGATCCCGTCCATCGCCACTGGGATGGTGGG GGCCCTCCTCTTGCTGCTGGTGGTGGCCCTGGGGATCGGCCTCTTCATGTGA

GCGGCCGCnrCTAGACCCGGGCTGCAGGAATTCGATATCAAGCTTATCGATAATC

AACCTCTGGATTACAAAATTTGTGAAAGATTGACTGGTATTCTTAACTATGTTGC TCCTTTTACGCTATGTGGATACGCTGCTTTAATGCCTTTGTATCATGCTATTGCTT CCCGTATGGCTTTCATTTTCTCCTCCTTGTATAAATCCTGGTTGCTGTCTCTTTAT GAGGAGTTGTGGCCCGTTGTCAGGCAACGTGGCGTGGTGTGCACTGTGTTTGCT GACGCAACCCCCACTGGTTGGGGCATTGCCACCACCTGTCAGCTCCTTTCCGGG ACTTTCGCTTTCCCCCTCCCTATTGCCACGGCGGAACTCATCGCCGCCTGCCTTG CCCGCTGCTGGACAGGGGCTCGGCTGTTGGGCACTGACAATTCCGTGGTGTTGT CGGGGAAATCATCGTCCTTTCCTTGGCTGCTCGCCTGTGTTGCCACCTGGATTCT GCGCGGGACGTCCTTCTGCTACGTCCCTTCGGCCCTCAATCCAGCGGACCTTCCT TCCCGCGGCCTGCTGCCGGCTCTGCGGCCTCTTCCGCGTCTTCGCCTTCGCCCTC AGACGAGTCGGATCTCCCTTTGGGCCGCCTCCCCGCATCGATACCGTCGACTAG CCGTACCTTTAAGACCAATGACTTACAAGGCAGCTGTAGATCTTAGCCACTTTTT A A A AGAAAAGGG G GGACTGGA AGGGCTAATTC ACTCCCAAAGAAGACAA GATC TGCTTTTTGCCTG7ACTGGGTCTCTCTGGTTAGACCAGATCTGAGCCTGGGAGCT CTCTGGCTAACTAGGGAACCCACTGCTTAAGCCTCAATAAAGCTTGCCTTGAGT GCTTCAAGTAGTGTGTGCCCGTCTGTTGTGTGACTCTGGTAACTAGAGATCCCTC AGACCCTTTTAGTCAGTGTGGAAAATCTCTAGCAGAATTCGATATCAAGCTTAT

CGATACCGTCGAC|CTCGAG|GGGGGGCCCGGTACCCAATTCGCCCTATAGTGAGT CGTATTACAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTG GCGTTACCC AACTTAATCGCCTTG C AGC A C ATCCCCCTTTCGCCAGCTG G CGTAA TAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGG CGAATGGAAATTGTAAGCGITAATATTTTGTI'AAAATTCGCGTTAAATTTTTGTT AAATCAGCTCATTTTTTAACCAATAGGCCGAAATCGGCAAAATCCC'ITATAAAT CAAAAGAATAGACCGAGATAGGGTTGAGTGTTGTTCCAGTTTGGAACAAGAGT CCACTATTAAAGAACGTGGACTCCAACGTCAAAGGGCGAAAAACCGTCTATCA GGGCGATGGCCCACTACGTGAACCATCACCCTAATCAAGTTTTTTGGGGTCGAG GTGCCGTAAAGCACTAAATCGGAACCCTAAAGGGAGCCCCCGATTTAGAGCTTG ACGGGGAAAGCCGGCGAACGTGGCGAGAAAGGAAGGGAAGAAAGCGAAAGGA GCGGGCGCTAGGGCGCTGGCAAGTGTAGCGGTCACGCTGCGCGTAACCACCAC ACCCGCCGCGCTTAATGCGCCGCTACAGGGCGCGTCAGGTGGCACTTTTCGGGG AAATGTGCGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTAT CCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAG AGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTG CCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGA TCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGAT CCT GAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTTAAAGTT CTGCTATGTGGCGCGGTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGT CGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAA AAGCATCTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACC ATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACGATCGGAGGACCGAA GGAGCTAACCGCTTTT RGCACAACATGGGGGATCATGTAACTCGCCTTGATCG TTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGA TGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTA CTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAG GACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGG AGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAA GCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGA ACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACT GTCAGACCAAGTTTACTCATATATACTTTAGATTGATTTAAAACTTCATTTTTAA TTTAAAAGGATCTAGGTGAAGATCCTTTTTGATAATCTCATGACCAAAATCCCTT AACGTGAGTTTTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGAT CTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACC ACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCG AAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAG CCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCT CTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCG GGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACG GGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAG ATACCTACAGCGTGAGCTATGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGG CGGACAGGTATCCGGTAAGCG GCA GGGTCGG AAC AG GAG AGCGCACG AGGGA GCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTC TGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAA AACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTC ACATGTTCTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTT GAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGT GAGCGAGGAAGCGGAAGAGCGCCCAATACGCAAACCGCCTCTCCCCGCGCGTT GGCCGATTCATTAATGCAGCTGGCACGACAGGTTTCCCGACTGGAAAGCGGGCA GTGAGCGCAACGCAA AATGTGAGTTAGCTCACTCATTAGGCACCCCAGGCTT TACACTTTATGCTTCCGGCTCGTATGTTGTGTGGAATTGTGAGCGGATAACAATT TCACACAGGAAACAGCTATGACCATGATTACGCCAAGCTCGAAATTAACCCTCA CTAAAGGGAACAAAAGCTGGAGCTCCACCGCGGTGGCGGC|CTCGAG|GTCGAGA TCCGGTCGACCAGCAACCATAGTCCCGCCCCTAACTCCGCCCATCCCGCCCCTA ACTCCGCCCAGTTCCGCCCATTCTCCGCCCCATGGCTGACTAATTTTTTTTATTTA TGCAGAGGCCGAGGCCGCCTCGGCCTCTGAGCTATTCCAGAAGTAGTGAGGAG GCTTTTTTGGAGGCCTAGGCTTTTGCAAAAAGCTTCGACGGTATCGATTGGCTCA TGTCCAACATTACCGCCATGTTGACATTGATTATTGACTAGTTATTAATAGTAAT CAATTACGGGGTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAAC TTACGGTAAATGGCCCGCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGT CAATAATGACGTATGTTCCCATAGTAACGCCAATAGGGACTTTCCATTGACGTC AATGGGTGGAGTATTTACGGTAAACTGCCCACTTGGCAGTACATCAAGTGTATC ATATGCCAAGTACGCCCCCTATTGACGTCAATGACGGTAAATGGCCCGCCTGGC ATTATGCCCAGTACATGACCTTATGGGACTTTCCTACTTGGCAGTACATCTACGT ATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACATCAATGGGCG TGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGTCAA TGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAACAA CTCCGCCCCATTGACGCAAATGGGCGGTAGGCGTGTACGGAATTCGGAGTGGCG AGCCCTCAGATCCTGCATATAAGCAGCTGCTTTTTGCCTGTACTGGGTCTCTCTG

Table 10

Leader R12- Hinge-CH2-CH3- CD28tm/41BB-Z-T2A-tEGFR (SEQ ID NO:38)

Leader

MLLLVTSLLLCELPHPAFLL1P

R12 scFv

QEQLVESGGRLVTPGGSLTLSCKASGFDFSAYYMSWVRQAPGKGLE IATIYPSSG KT ATWNGRFTISSDNAQNT^LQMNSLTAADRATYFCARDSYADDGALFNI WGPGTLVTISSGGGGSGGGGSGGGGSELVLTQSPSVSAALGSPAKITCTLSSAHKTD TIDWYQQLQGEAPRYLMQVQSDGSYTI RPGVPDRFSGSSSGADRYLIIPSVQADDE ADYYCGADYIG GYVFG GGTQLT VTG

Hinge Spacer

ESKYGPPCPPCP CH2

APEFLGGPS VFLFPPKPKDTLMISR PEVTQVV VDVSOEDPEVOFN W Y VDGVEVHN AKT PREEQFNSTYRVVSVLTVLHQD WLNGKEYT ^ VSN KGLPS S1EKTIS AK CH3

GQPREPQVYTLPPSQEEMTKNQVSLT|C|LV GFYPSDIAVEWESNGQPENNYKTTPP VLDSDGSFFLYSRLTVDKSR QEGNWSGSVMHEALH HYTQKSLSLSLGK

CD28

MFWVLVVVGGVLACYSLLVTVAFIIFWV

4-1 BB

I RGR KLLYIFKQPFMRPVQTTQEEDGCSCRFPEEEEGGCEL CD3 zeta

RVKFSRSADAPAYQQGQNQLYNELNLGRREEYDVLDKRRGRDPEMGGKPRRKNP QEGLYNELQ D MAEA YSE1GM KGERRRGKGHDGLYQGLSTATKDTYDALHMQ ALPPR

T2A

LEGGGEGRG SLLTCG D VEENPGPR

tEGFR

MLLLVTSLLLCELPHPAFLLfPR VCNGlGIGEFKDSLSlNATNl HFKNCTSISGDLHI LPV AF GDS FTHTPPLDPQELDIL TVKE1TGFLL1QAWPENRTDLHAFENLEI 1 RG RT QHGQFSLAVVSLNITSLGLRSL EISDGDVITSGNKNLCYANT1NW LFGTSGQK TKIIS RGENSCKATGQVCHALCSPEGCWGPEPRDCVSCRMVSRGRECVDKCNLLE GEPREFVENSEClQCHPECLPQAMNiTCTGRGPDNCIQCAHYlDGPHCVKTCPAGVM GENNTLVW YADAGHVCHLCHPNCTYGCTGPGLEGCPTNGP IPS1ATGMVGALE LLLVVALGIGLFM Table 11

R12 intermediate spacer CAR: PJ_R12~CH3-41BB-Z-T2A-tEGFR (SEQ ID NO:39)

GTTAGACCAGATCTGAGCCTGGGAGCTCTCTGGCTAACTAGGGAACCCACTGCT TAAGCCTCAATAAAGCTTGCCTTGAGTGCTTCAAGTAGTGTGTGCCCGTCTGTTG TGTGACTCTGGTAACTAGAGATCCCTCAGACCCTTTTAGTCAGTGTGGAAAATC TCTAGCAGTGGCGCCCGAACAGGGACTTGAAAGCGAAAGGGAAACCAGAGGAG CTCTCTCGACGCAGGACTCGGCTTGCTGAAGCGCGCACGGCAAGAGGCGAGGG GCGGCGACTGGTGAGTACGCCAAAAATTTTGACTAGCGGAGGCTAGAAGGAGA GAGATGGGTGCGAGAGCGTCAGTATTAAGCGGGGGAGAATTAGATCGATGGGA AAAAATTCGGTTAAGGCCAGGGGGAAAGAAAAAATATAAATTAAAACATATAG TATGGGCAAGCAGGGAGCTAGAACGATTCGCAGTTAATCCTGGCCTGTTAGAAA CATCAGAAGGCTGTAGACAAATACTGGGACAGCTACAACCATCCCTTCAGACA GGATCAGAAGAACTTAGATCATTATATAATACAGTAGCAACCCTCTATTGTGTG CATCAAAGGATAGAGATAAAAGACACCAAGGAAGCTTTAGACAAGATAGAGGA AGAGCAAAACAAAAGTAAGAAAAAAGCACAGCAAGCAGCAGCTGACACAGGA CACAGCAATCAGGTCAGCCAAAATTACCCTATAGTGCAGAACATCCAGGGGCA AATGGTACATCAGGCCATATCACCTAGAACTTTAAATGCATGGGTAAAAGTAGT AGAAGAGAAGGCTTTCAGCCCAGAAGTGATACCCATGTTTTCAGCATTATCAGA AGGAGCCACCCCACAAGATTTAAACACCATGCTAAACACAGTGGGGGGACATC AAGCAGCCATGCAAATGTTAAAAGAGACCATCAATGAGGAAGCTGCAGGCAAA GAGAAGAGTGGTGCAGAGAGAAAAAAGAGCAGTGGGAATAGGAGCTTTGTTCC TTGGGTTCTTGGGAGCAGCAGGAAGCACTATGGGCGCAGCGTCAATGACGCTG ACGGTACAGGCCAGACAATTATTGTCTGGTATAGTGCAGCAGCAGAACAATTTG CTGAGGGCTATTGAGGCGCAACAGCATCTGTTGCAACTCACAGTCTGGGGCATC AAGCAGCTCCAGGCAAGAATCCTGGCTGTGGAAAGATACCTAAAGGATCAACA GCTCCTGGGGATTTGGGGTTGCTCTGGAAAACTCATTTGCACCACTGCTGTGCCT TGGATCTACAAATGGCAGTATTCATCCACAATTTTAAAAGAAAAGGGGGGATTG GGGGGTACAGTGCAGGGGAAAGAATAGTAGACATAATAGCAACAGACATACAA ACTAAAGAATTACAAAAACAAATTACAAAAATTCAAAATTTTCGGGTTTATTAC AGGGACAGCAGAGATCCAGTTTGGGGATCAATTGCATGAAGAATCTGCTTAGG GTTAGGCGTTTTGCGCTGCTTCGCGAGGATCTGCGATCGCTCCGGTGCCCGTCA GTGGGCAGAGCGCACATCGCCCACAGTCCCCGAGAAGTTGGGGGGAGGGGTCG GCAATTGAACCGGTGCCTAGAGAAGGTGGCGCGGGGTAAACTGGGAAAGTGAT GTCGTGTACTGGCTCCGCCTTTTTCCCGAGGGTGGGGGAGAACCGTATATAAGT GCAGTAGTCGCCGTGAACGTTCTTTTTCGCAACGGGTTTGCCGCCAGAACACAG CTGAAGCTTCG AG G G G CTCGC ATCTCTCCTTCACG CGCCCGCCGCCCTACCTG A GGCCGCCATCCACGCCGGTTGAGTCGCGTTCTGCCGCCTCCCGCCTGTGGTGCC TCCTGAACTGCGTCCGCCGTCTAGGTAAGTTTAAAGCTCAGGTCGAGACCGGGC CTTTGTCCGGCGC'FCCCTTGGAGCCTACCTAGACTCAGCCGGCTCTCCACGCTTT GCCTGACCCTGCTTG CTCAACTCTACGTCTTrGTTTCGTTTTCTGTTCTGCG CCGT TACAGATCCAAGCTGTGACCGGCGCCTACG

GCTAGC G AATTCCTCG AGGCC

R12 ScFv

ACC^TGCTGCTGCTGGTGACAAGCCTGCTGCTGTGCGAGCTGCCCCACCCCGCC

TTTCTGCTG ATCCCCCAGG AAC AG CTCGTCG A A AG CGGCG G CAG ACTGGTG AC A

Hinge Spacer

CH3

4-1 BB

CD3zeta

Τ2Α

tEGFR

CTCCTCCTCTjGGATCG|ACAGGAACTGGATATTCTGAAAACCGTAAAGGAAATCA CAGGGTTTTTGCTGATTCAGGCTTGGCCTGAAAACAGGACGGACCTCCATGCCT TTGAGAACCTAGAAATCATACGCGGCAGGACCAAGCAACATGGTCAGTTTTCTC TTGCAGTCGTCAGCCTGAACATAACATCCTTGGGATTACGCTCCCTCAAGGAGA TAAGTGATGGAGATGTGATAATTTCAGGAAACAAAAATTTGTGCTATGCAAATA CAATAAACTGGAAAAAACTGTTTGGGACCTCCGGTCAGAAAACCAAAATTATA AGCAACAGAGGTGAAAACAGCTGCAAGGCCACAGGCCAGGTCTGCCATGCCTT GTGCTCCCCCGAGGGCTGCTGGGGCCCGGAGCCCAGGGACTGCGTCTCTTGCCG GAATGTCAGCCGAGGCAGGGAATGCGTGGACAAGTGCAACCTTCTGGAGGGTG AGCCAAGGGAGTTTGTGGAGAACTCTGAGTGCATACAGTGCCACCCAGAGTGC CTGCCTCAGGCCATGAACATCACCTGCACAGGACGGGGACCAGACAACTGTATC CAGTGTGCCCACTACATTGACGGCCCCCACTGCGTCAAGACCTGCCCGGCAGGA GTCATGGGAGAAAACAACACCCTGGTCTGGAAGTACGCAGACGCCGGCCATGT GTGCCACCTGTGCCATCCAAACTGCACCTACGGATGCACTGGGCCAGGTCTTGA AGGCTGTCCAACGAATGGGCCTAAGATCCCGTCCATCGCCACTGGGATGGTGGG GGCCCTCCTCTTGCTGCTGGTGGTGGCCCTGGGGATCGGCCTCTTCATGlil GCGGCCGCTCTAGACCCGGGCTGCAGGAATTCGATATCAAGCTTATCGATAATC

AACCTCTGGATTACAAAATTTGTGAAAGATTGACTGGTATTCTTAACTATGTTGC TCCTTTTACGCTATGTGGATACGCTGCTTTAATGCCTTTGTATCATGCTATTGCTT CCCGTATGGCTTTCATTTTCTCCTCCTTGTATAAATCCTGGTTGCTGTCTCTTTAT GAGGAGTTGTGGCCCGTTGTCAGGCAACGTGGCGTGGTGTGCACTGTGTTTGCT GACGCAACCCCCACTGGTTGGGGCATTGCCACCACCTGTCAGCTCCTTTCCGGG ACTTTCGCTTTCCCCCTCCCTATTGCCACGGCGGAACTCATCGCCGCCTGCCTTG CCCGCTGCTGGACAGGGGCTCGGCTGTTGGGCACTGACAATTCCGTGGTGTTGT CGGGGAAATCATCGTCCTTTCCTTGGCTGCTCGCCTGTGTTGCCACCTGGATTCT GCGCGGGACGTCCTTCTGCTACGTCCCTTCGGCCCTCAATCCAGCGGACCTTCCT TCCCGCGGCCTGCTGCCGGCTCTGCGGCCTCTTCCGCGTCTTCGCCTTCGCCCTC AGACGAGTCGGATCTCCCTTTGGGCCGCCTCCCCGCATCGATACCGTCGACTAG CCGTACCTTTAAGACCAATGACTTACAAGGCAGCTGTAGATCTTAGCCACTTTTT AAAAGAAAAGGGGGGACTGGAAGGGCTAATTCACTCCCAAAGAAGACAAGATC TGCTTTTTGGCTGTACTGGGTCTCTCTGGTTAGACCAGATCTGAGCCTGGGAGCT CTCTGGCTAACTAGGGAACCCACTGCTTAAGCCTCAATAAAGCTTGCCTTGAGT GCTTCAAGTAGTGTGTGCCCGTCTGTTGTGTGACTCTGGTAACTAGAGATCCCTC AGACCCTTTTAGTCAGTGTGGAAAATCTCTAGCAGAATTCGATATCAAGCTTAT

CGATACCGTCGACjCTCGAGjGGGGGGCCCGGTACCCAATTCGCCCTATAGTGAGT CGTATTACAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTG GCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAA TAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGG CGAATGGAAATTGTAAGCGTTAATATTTTGTTAAAATTCGCGTTAAATTTTTGTT AAATCAGCTCATTTTTTAACCAATAGGCCGAAATCGGCAAAATCCCTTATAAAT CAAAAGAATAGACCGAGATAGGGTTGAGTGTTGTTCCAGTTTGGAACAAGAGT CCACTATTAAAGAACGTGGACTCCAACGTCAAAGGGCGAAAAACCGTCTATCA GGGCGATGGCCCACTACGTGAACCATCACCCTAATCAAGTTTTTTGGGGTCGAG GTGCCGTAAAGC ACT AAATCGG A A CCCTA AAG G G AGCCCCCG ATTT AG AGCTTG ACGGGGAAAGCCGGCGAACGTGGCGAGAAAGGAAGGGAAGAAAGCGAAAGGA GCGGGCGCTAGGGCGCTGGCAAGTGTAGCGGTCACGCTGCGCGTAACCACCAC ACCCGCCGCGCTTAATGCGCCGCTACAGGGCGCGTCAGGTGGCACTTTTCGGGG AAATGTGCGCGGAACCCCTATTTGTTTATTI TTCTAAATACATTCAAATATGTAT CCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAG AGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTG CCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGA TCAGTTG GGTG CACG AGTGGGTTACATCGAACTGGATCTC AACAGCGGTAAG AT CCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTTAAAGTT CTGCTATGTGGCGCGGTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGT CGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAA AAGCATCTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACC ATGAGTG ATA ACACTGCG GCC A ACTTACTTCTGAC AACGATCGGAG GACCG AA GGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGATCG TTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGA TGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTA CTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAG GACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGG AGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAA GCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGA ACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACT GTCAGACCAAGTTTACTCATATATACTTTAGATTGATTTAAAACTTCATTTTTAA TTTAAAAGGATCTAGGTGAAGATCCTTTTTGATAATCTCATGACCAAAATCCCTT AACGTGAGTTTTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGAT CTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACC ACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCG AAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAG CCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCT CTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCG GGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACG GGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAG ATACCTACAGCGTGAGCTATGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGG CGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGA GCTTCC AGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGG GTTTCGCC A CCTC TGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAA AACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTC ACATGTTCTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTT GAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGT GAGCGAGGAAGCGGAAGAGCGCCCAATACGCAAACCGCCTCTCCCCGCGCGTT GGCCGATTCATTAATGCAGCTGGCACGACAGGTTTCCCGACTGGAAAGCGGGCA GTGAGCGCAACGCAATTAATGTGAGTTAGCTCACTCATTAGGCACCCCAGGCTT TACACTTTATGCTTCCGGCTCGTATGTTGTGTGGAATTGTGAGCGGATAACAATT TCACACAGGAAACAGCTATGACCATGATTACGCCAAGCTCGAAATTAACCCTCA

CTAAAGGGAACAAAAGCTGGAGCTCCACCGCGGTGGCGGC|CTCGAG|GTCGAGA TCCGGTCGACCAGCAACCATAGTCCCGCCCCTAACTCCGCCCATCCCGCCCCTA ACTCCGCCCAGTTCCGCCCATTCTCCGCCCCATGGCTGACTAATT'nTTTTATTTA TGCAGAGGCCGAGGCCGCCTCGGCCTCTGAGCTATTCCAGAAGTAGTGAGGAG GCTTTTTTGGAGGCCTAGGCTTTTGCAAAAAGCTTCGACGGTATCGATTGGCTCA TGTCCAACATTACCGCCATGTTGACATTGATTATTGACTAGTTATTAATAGTAAT CAATTACGGGGTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAAC TTACGGTAAATG G CCCG CCTGGCTG ACCGCCCAACG ACCCCCGCCC ATTG ACGT CAATAATGACGTATG ^' I CCCATAGTAACGCCAATAGGGACTTTCCATTGACGTC AATGGGTGGAGTATTTACGGTA A ACTGCCC ACTTG G C AGTACATCAAGTGTATC ATATGCCAAGTACGCCCCCTATTGACGTCAATGACGGTAAATGGCCCGCCTGGC ATTATGCCCAGTACATGACCT ATGGGACTTTCCTACTTGGCAGTACATCTACGT ATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACATCAATGGGCG TGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGTCAA TGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAACAA CTCCGCCCCATTGACGCAAATGGGCGGTAGGCGTGTACGGAATTCGGAGTGGCG AGCCCTCAGATCCTGCATATAAGCAGCTGCTTTTTGCCTGTACTGGGTCTCTCTG

Table 12

Leader JR.12- Hinge- CH3- CD28tm/41BB-Z-T2A-tEGFR (SEQ ID NO:40)

Leader

MLLLVTSLLLCELPHPAFLLTP

R12 scFV

QEQLVESGGRLVTPGGSLTLSCKASGFDFSAYYMSWVRQAPGKGLEWIATIYPSSG KTYYATWVNGPJ^TISSDNAQNTVDLQMNSLTAADRATYFCARDSYADDGALFNl WGPGTLVTISSGGGGSGGGGSGGGGSELVLTQSPSVSAALGSPAKITCTLSSAHKTD TIDWYQQLQGEAPRYLMQVQSDGSYT RPGVPDRFSGSSSGADRYLIIPSVQADDE ADYYCGADYIGGYVFGGGTQLTVTG

Hinge Spacer

ESKYGPPCPPCP

C.H.3

GQPREPQVYTLPPSQEEMTKNQVSLT@LV GFYPSDIAVEWESNGQPEN Y TTPP VLDSDGSFFLYSRLTVDKSRWQEGNVFSgsVMHEALH HYTQKSLSLSLGK

CD28tm

MFWVLVVVGGVLACYSLLVTVAFIIFWV

4-1 BB

KRGRKKLLYIFKQPFMRPVQTTQEEDGCSCRFPEEEEGGCEL CD3 zeta

RVK-FSRSADAPAYQQGQNQLY ELNLGRREEYDVLDKRRGRDPEMGGKPR]^^

QEGLYNELQKDKMAEAYSEIGM GERRRGKGHDGLYQGLSTATKDTYDALHMQ

ALPPR

T2A

LEGGGEGRG SLLTCGD VEENPGPR

tEGFR

MLLLVTSLLLCELPHPAFLLIPRK.VCNGIG1GEFKDSLSINATN1KJ-LFKNCTSISGD LHI LPVAFRGDSFTHTPPLDPQELDIL TV EITGFLLIQAWPENRTDLHAFE LEIIRGRT KQHGQFSLAVVSLNlTSLGLRSL EISDGDVIISGNKNLCYANTr W LFGTSGQ TKIISNRGENSCKATGQVCHALCSPEGCWGPEPRDCVSCRNVSRGRECVDKCNLLE GEPREF VEN SECIQCI IPECLPQ A MN ITCTGRG PDNCIQCAH Y I DGPHCVKTCPAG VM GENNTLVWKYADAGHVCHLCHPNCTYGCTGPGLEGCPTNGP IPSIATGMVGALL LLLVVALGIGLFM Table 13

R12 short spacer CAR: PJ_R12-Hinge-41BB-Z-T2A-tEGFR (SEQ UD NO:41)

GTTAGACCAGATCTGAGCCTGGGAGCTCTCTGGCTAACTAGGGAACCCACTGCT TAAGCCTCAATAAAGCTTGCCTTGAGTGCTTCAAGTAGTGTGTGCCCGTCTGTTG TGTGACTCTGGTAACTAGAGATCCCTCAGACCCTTTTAGTCAGTGTGGAAAATC TCTAGCAGTGGCGCCCGAACAGGGACTTGAAAGCGAAAGGGAAACCAGAGGAG CTCTCTCGACGCAGGACTCGGCTTGCTGAAGCGCGCACGGCAAGAGGCGAGGG GCGGCGACTGGTGAGTACGCCAAAAATTTTGACTAGCGGAGGCTAGAAGGAGA GAGATGGGTGCGAGAGCGTCAGTATTAAGCGGGGGAGAATTAGATCGATGGGA AAAAATTCGGTTAAGGCCAGGGGGAAAGAAAAAATATAAATTAAAACATATAG TATGGGCAAGCAGGGAGCTAGAACGATTCGCAGTTAATCCTGGCCTGTTAGAAA CATCAGAAGGCTGTAGACAAATACTGGGACAGCTACAACCATCCCTTCAGACA GGATCAGAAGAACTTAGATCATTATATAATACAGTAGCAACCCTCTATTGTGTG CATCAAAGGATAGAGATAAAAGACACCAAGGAAGCTTTAGACAAGATAGAGGA AGAGCAAAACAAAAGTAAGAAAAAAGCACAGCAAGCAGCAGCTGACACAGGA CACAGCAATCAGGTCAGCCAAAATTACCCTATAGTGCAGAACATCCAGGGGCA AATGGTACATCAGGCCATATCACCTAGAACTTTAAATGCATGGGTAAAAGTAGT AGAAGAGAAGGCTTTCAGCCCAGAAGTGATACCCATGTTTTCAGCATTATCAGA AGGAGCCACCCCACAAGATTTAAACACCATGCTAAACACAGTGGGGGGACATC AAGCAGCCATGCAAATGTTAAAAGAGACCATCAATGAGGAAGCTGCAGGCAAA GAGAAGAGTGGTGCAGAGAGAAAAAAGAGCAGTGGGAATAGGAGCTTTGTTCC TTGGGTTCTTGGGAGCAGCAGGAAGCACTATGGGCGCAGCGTCAATGACGCTG ACGGTACAGGCCAGACAATTATTGTCTGGTATAGTGCAGCAGCAGAACAATTTG CTGAGGGCTATTGAGGCGCAACAGCATCTGTTGCAACTCACAGTCTGGGGCATC AAGCAGCTCCAGGCAAGAATCCTGGCTGTGGAAAGATACCTAAAGGATCAACA GCTCCTGGGGATTTGGGGTTGCTCTGGAAAACTCATTTGCACCACTGCTGTGCCT TGGATCTACAAATGGCAGTATTCATCCACAATTTTAAAAGAAAAGGGGGGATTG GGGGGTACAGTGCAGGGGAAAGAATAGTAGACATAATAGCAACAGACATACAA ACTAAAGAATTACAAAAACAAATTACAAAAATTCAAAATTTTCGGGTTTATTAC AGGGACAGCAGAGATCCAGTTTGGGGATCAATTGCATGAAGAATCTGCTTAGG GTTAGGCGTTTTGCGCTGCTTCGCGAGGATCTGCGATCGCTCCGGTGCCCGTCA GTGGGCAGAGCGCACATCGCCCACAGTCCCCGAGAAGTTGGGGGGAGGGGTCG GCAATTGAACCGGTGCCTAGAGAAGGTGGCGCGGGGTAAACTGGGAAAGTGAT GTCGTGTACTGGCTCCGCCTTTTTCCCGAGGGTGGGGGAGAACCGTATATAAGT GCAGTAGTCGCCGTGAACGTTCTTTTTCGCAACGGGTTTGCCGCCAGAACACAG CTGAAGCTTCGAGGGGCTCGCATCTCTCCTTCACGCGCCCGCCGCCCTACCTGA GG CCGCCATCC ACGCCGGTTG AG TCGCGTTCTGCCGCCTCCC G CCTG TGGTGCC TCCTGAACTGCGTCCGCCGTCTAGGTAAGTTTAAAGCTCAGGTCGAGACCGGGC CTTTG TCCGG CGCTCCCTTGG AGCCTACCTAGACTC AGCCG GCTCTCC ACGCTTT GCCTG ACCCTGCTTGCTC A ACTCTACGTCTTTGTTTCGTTTTCTGTTCTG CGCCGT TACAGATCCAAGCTGTGACCGGCGCCTACG

GCTAG H

R12 scFM

ACC[ATG]CTGCTGCTGGTGACAAGCCTGCTGCTGTGCGAGCTGCCCCACCCCGCC TTTCTGCTGATCCCCCAGGAACAGCTCGTCGAAAGCGGCGGCAGACTGGTGACA CCTGGCGGCAGCCTGACCCTGAGCTGCAAGGCCAGCGGCTTCGACTTCAGCGCC TACTACATGAGCTGGGTCCGCCAGGCCCCTGGCAAGGGACTGGAATGGATCGCC ACCATCTACCCCAGCAGCGGCAAGACCTACTACGCCACCTGGGTGAACGGACG GTTCACCATCTCCAGCGACAACGCCCAGAACACCGTGGACCTGCAGATGAACA GCCTGACAGCCGCCGACCGGGCCACCTACTTTTGCGCCAGAGACAGCTACGCCG ACGACGGCGCCCTGTTCAACATCTGGGGCCCTGGCACCCTGGTGACAATCTCTA GCGGCGGAGGCGGATCTGGTGGCGGAGGAAGTGGCGGCGGAGGATCTGAGCTG GTGCTG ACCCAGAGCCCCTCTGTGTCTGCTG CCCTG GGA AGCCCTGCCAAGATC ACCTGTACCCTGAGCAGCGCCCACAAGACCGACACCATCGACTGGTATCAGCA GCTGCAGGGCGAGGCCCCCAGATACCTGATGCAGGTGCAGAGCGACGGCAGCT ACACCAAGAGGCCAGGCGTGCCCGACCGGTTCAGCGGATCTAGCTCTGGCGCC GACCGCTACCTGATCATCCCCAGCGTGCAGGCCGATGACGAGGCCGATTACTAC TGTGGCGCCGACTACATCGGCGGCTACGTGTTCGGCGGAGGCACCCAGCTGACC

4-1BB

ATGTTCTGGGTGCTGGTGGTGGTGGGCGGGGTGCTGGCCTGCTACAGCCTGCTG GTGACAGTGGCCTTCATCATCTTTTGGGTGAAACGGGGCAGAAAGAAACTCCTG TATATATTCAAACAACCATTTATGAGACCAGTACAAACTACTCAAGAGGAAGAT GGCTGTAGCTGCCGATTTCCAGAAGAAGAAGAAGGAGGATGTGAACTG

CD3 zeta

CGGGTGAAGTTCAGCAGAAGCGCCGACGCCCCTGCCTACCAGCAGGGCCAGAA TCAGCTGTACAACGAGCTGAACCTGGGCAGAAGGGAAGAGTACGACGTCCTGG ATAAGCGGAGAGGCCGGGACCCTGAGATGGGCGGCAAGCCTCGGCGGAAGAAC CCCCAGGAAGGCCTGTATAACGAACTGCAGAAAGACAAGATGGCCGAGGCCTA CAGCGAGATCGGCATGAAGGGCGAGCGGAGGCGGGGCAAGGGCCACGACGGC CTGTATCAGGGCCTGTCCACCGCCACCAAGGATACCTACGACGCCCTGCACATG CAGGCCCTGCCCCCAAGG

Τ2Α

CTCGAGGGCGGCGGAGAGGGCAGAGGAAGTCTTCTAACATGCGGTGACGTGGA

GGAGAATCCCGGCCCTAGG

tEGFR

ATGCTTCTCCTGGTGACAAGCCTTCTGCTCTGTGAGTTACCACACCCAGCATTCC TCCTGATCCCACGCAAAGTGTGTAACGGAATAGGTATTGGTGAATTTAAAGACT CACTCTCCATAAATGCTACGAATATTAAACACTTCAAAAACTGCACCTCCATCA GTGGCGATCTCCACATCCTGCCGGTGGCATTTAGGGGTGACTCCTTCACACATA

CTCCTCCTCT^GJ ¾ACAGGAACTGGATATTCTGAAAACCGTAAAGGAAATCA CAGGGTTTTTGCTGATTCAGGCTTGGCCTGAAAACAGGACGGACCTCCATGCCT TTGAGAACCTAGAAATCATACGCGGCAGGACCAAGCAACATGGTCAGTTTTCTC TTGCAGTCGTCAGCCTGAACATAACATCCTTGGGATTACGCTCCCTCAAGGAGA TAAGTGATGGAGATGTGATAATTTCAGGAAACAAAAATTTGTGCTATGCAAATA CAATAAACTGGAAAAAACTGTTTGGGACCTCCGGTCAGAAAACCAAAATTATA AGCAACAGAGGTGAAAACAGCTGCAAGGCCACAGGCCAGGTCTGCCATGCCTT GTGCTCCCCCGAGGGCTGCTGGGGCCCGGAGCCCAGGGACTGCGTCTCTTGCCG GAATGTCAGCCGAGGCAGGGAATGCGTGGACAAGTGCAACCTTCTGGAGGGTG AGCCAAGGGAGTTTGTGGAGAACTCTGAGTGCATACAGTGCCACCCAGAGTGC CTGCCTCAGGCCATGAACATCACCTGCACAGGACGGGGACCAGACAACTGTATC CAGTGTGCCCACTACATTGACGGCCCCCACTGCGTCAAGACCTGCCCGGCAGGA GTCATGGGAGAAAACAACACCCTGGTCTGGAAGTACGCAGACGCCGGCCATGT GTGCCACCTGTGCCATCCAAACTGCACCTACGGATGCACTGGGCCAGGTCTTGA AGGCTGTCCAACGAATGGGCCTAAGATCCCGTCCATCGCCACTGGGATGGTGGG GGCCCTCCTCTTGCTGCTGGTGGTGGCCCTGGGGATCGGCCTCTTCATGiSi

GCGGCCGCrrCTAGACCCGGGCTGCAGGAATTCGATATCAAGCTTATCGATAATC

AACCTCTGGATTACAAAATTTGTGAAAGATTGACTGGTATTCTTAACTATGTTGC TCCTTTTACGCTATGTGGATACGCTGCTTTAATGCCTTTGTATCATGCTATTGCTT CCCGTATGGCTTTCATTTTCTCCTCCTTGTATAAATCCTGGTTGCTGTCTCTTTAT GAGGAGTTGTGGCCCGTTGTCAGGCAACGTGGCGTGGTQTGCACTGTGTTTGCT GACGCAACCCCCACTGGTTGGGGCATTGCCACCACCTGTCAGCTCCTTTCCGGG ACTTTCGCTTTCCCCCTCCCTATTGCCACGGCGGAACTCATCGCCGCCTGCCTTG CCCGCTGCTGGACAGGGGCTCGGCTGTTGGGCACTGACAATTCCGTGGTGTTGT CG GG G A A ATC ATCGTCCTTTCCTTGGCTGCTCGCCTGTGTTGCCACCTGGATTCT GCGCGGGACGTCCTTCTGCTACGTCCCTTCGGCCCTCAATCCAGCGGACCTTCCT TCCCGCGGCCTGCTGCCGGCTCTGCGGCCTCTTCCGCGTCTTCGCCTTCGCCCTC AGACGAGTCGGATCTCCCTTTGGGCCGCCTCCCCGCATCGATACCGTCGACTAG CCGTACCTTTAAGACCAATGACTTACAAGGCAGCTGTAGATCTTAGCCACTTTTT AAAAGAAAAGGGGGGACTGGAAGGGCTAATTCACTCCCAAAGAAGACAAGATC TGCTTTTTGCCTGTACTGGGTCTCTCTGGTTAGACCAGATCTGAGCCTGGGAGCT CTCTGG CTAACTAGGGAACCCACTGCTTAAGCCTC A ATA A AGCTTGCCTTGAG T GCTTCAAGTAGTGTGTGCCCGTCTGTTGTGTGACTCTGGTAACTAGAGATCCCTC AGACCCTTTTAGTCAGTGTGGAAAATCTCTAGCAGAATTCGATATCAAGCTTAT CGATACCGTCGAC|CTCGAG|GGGGGGCCCGGTACCCAATTCGCCCTATAGTGAGT CGTATTACAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTG GCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAA TAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGG CGAATGGAAATTGTAAGCGTTAATATTTTGTTAAAATTCGCGTTAAATTTTTGTT AAATCAGCTCATTTTTTAACCAATAGGCCGAAATCGGCAAAATCCCTTATAAAT CAAAAGAATAGACCGAGATAGGGTTGAGTGTTGTTCCAGTTTGGAACAAGAGT CCACTATTAAAGAACGTGGACTCCAACGTCAAAGGGCGAAAAACCGTCTATCA GGGCGATGGCCCACTACGTGAACCATCACCCTAATCAAGTTTTTTGGGGTCGAG GTGCCGTAAAGCACTAAATCGGAACCCTAAAGGGAGCCCCCGATTTAGAGCTTG ACGGGGAAAGCCGGCGAACGTGGCGAGAAAGGAAGGGAAGAAAGCGAAAGGA GCGGGCGCTAGGGCGCTGGCAAGTGTAGCGGTCACGCTGCGCGTAACCACCAC ACCCGCCGCGCTT AATGCGCCG CTACAG GGCG CGTC AGGTGGC ACTTTTCGGGG AAATGTGCGCGGAACCCCTATTTGTTl ^{^} ATTTTTCTAAATACATTCAAATATGTAT CCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAG AGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTG CCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGA TCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGAT CCTTGAGAGTTTTCGGCCCGAAGAACGTTTTCCAATGATGAGCACTTTTAAAGTT CTGCTATGTGGCGCGGTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGT CGCCGCATACACTATTCTCAGAATGACTTGGT'FGAGTACTCACCAGTCACAGAA AAGCATCTTACGGATGGCA TGACAGTAAGAGAATTATGCAGTGCTGCCATAACC ATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACGATCGGAGGACCGAA GGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGATCG TTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGA TGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTA CTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAG GACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGG AGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAA GCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGA ACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACT GTCAGACCAAGTTTACTCATATATACTTTAGATTGATTTAAAACTTCATTTTTAA TTTAAAAGGATCTAGGTGAAGATCCTTTTTGATAATCTCATGACCAAAATCCCTT AACGTGAGTTTTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGAT CTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACC ACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCG AAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAG CCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCT CTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCG GGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACG GGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAG ATACCTACAGCGTGAGCTATGAGA A AGCGCCACGCTTCCCG AAG G GAG AAAGG CGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGA GCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTC TGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAA AACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTC AC ATGTTCTTTCCTGCGTTATCCCCTG ATTCTG TGG ATAACCGTATTACCG CCTTT GAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGT GAGCGAGGAAGCGGAAGAGCGCCCAATACGCAAACCGCCTCTCCCCGCGCGTT GG CCG ATTCATTAATGC AGCTGG CACGACAGGTTTCCCGACTGGAAAGCGGGCA GTGAGCGCAACGCAATTAATGTGAGTTAGCTCACTCATTAGGCACCCCAGGCTT TACACTTTATGCTTCCGGCTCGTATGTTGTGTGGAATTGTGAGCGGATAACAATT TCACACAGGAAACAGCTATGACCATGATTACGCCAAGCTCGAAATTAACCCTCA CTAAAGGGAACAAAAGCTGGAGCTCCACCGCGGTGGCGGC^TCGA^TCGAGA TCCGGTCGACCAGCAACCATAGTCCCGCCCCTAACTCCGCCCATCCCGCCCCTA ACTCCGCCCAGTTCCGCCCATTCTCCGCCCCATGGCTGACTAATTTTTTTTATTTA TGCAGAGGCCGAGGCCGCCTCGGCCTCTGAGCTATTCCAGAAGTAGTGAGGAG GCTTTTTTGGAGGCCTAGGCTTTTGCAAAAAGCTTCGACGGTATCGATTGGCTCA TGTCCAACATTACCGCCATGTTGACATTGATTATTGACTAGTTATTAATAGTAAT CAATTACGGGGTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAAC TTACGGTAAATGGCCCGCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGT CAATAATGACGTATGTTCCCATAGTAACGCCAATAGGGACTTTCCATTGACGTC AATGGGTGGAGTATTTACGGTAAACTGCCCACTTGGCAGTACATCAAGTGTATC ATATGCCAAGTACGCCCCCTATTGACGTCAATGACGGTAAATGGCCCGCCTGGC ATTATGCCCAGTACATGACCTTATGGGACTTTCCTACTTGGCAGTACATCTACGT ATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACATCAATGGGCG TGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGTCAA TGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAACAA CTCCGCCCCATTGACGCAAATGGGCGGTAGGCGTGTACGGAATTCGGAGTGGCG AGCCCTCAGATCCTGCATATAAGCAGCTGCTTTTTGCCTGTACTGGGTCTCTCTG Table 14

Leader R12 - CD28tm/41BB-Z-T2A-tEGFR(SEQ ID NO:42)

Leader

MLLLVTSLLLCELPHPAFLLIP

scFv R12

QEQLVESGGRLVTPGGSLTLSCKASGFDFSAYYMSWVRQAPGKGLEW1ATIYPSSG

KTYYATWVNGPJ?TISSDNAQNTVDLQM SLTAADRATYFCARDSYADDGALFNT

WGPGTLVTISSGGGGSGGGGSGGGGSELVLTQSPSVSAALGSPAKITCTLSSAHKTD

TID WYQQLQGEAPR YLMQ VQSDG SYT RPGVPDRFS GS SSGADRYLIIPS VQADDE

ADYYCGADYIGGYVFGGGTQLTVTG

Hinge/spacer

ESKYGPPCPPCP

CD28tm

MFWVLWVGGVLACYSLLVTVAF1IFWV

4-1BB

RGR KLLYIFKQPFMRPVQTTQEEDGCSCRFPEEEEGGCEL CD3zeta

RVKFSRSADAPAYQQGQNQLYNELNLGRREEYDVLDKRRGRDPEMGG PRRlONiP

QEGLYNELQ D MAEAYSEIGM GERRRGKGHDGLYQGLSTAT DTYDALHMQ

ALPPR

T2A

LEGGGEGRGSLLTCGDVEEN PGPR

tEGFR

MLLLVTSLLLCELPFIPAFLLJPR VCNGIGIGEF DSLSINATNn'CHFI'iNCTSISGDLHI

LPVAFRGDSFTHTPPLDPQELDILKTVKEITGFLLIQAWPENRTDLHAFE LEIIRGRT

KQFIGQFSLAVVSLNITSLGLRSLKEISDGDVIISGN NLCYANTfNWK LFGTSGQK

T I1SNRGENSCKATGQVCHALCSPEGCWGPEPRDCVSCRNVSRGRECVD CNLLE

GEPREFVENSECIQCHPECLPQA NITCTGRGPDNCIQCAIIYIDGPHCVKTCPAGVM

GENNTLVWKYADAGHVCHLCHPNCTYGCTGPGLEGCPT GP IPSIATGMVGALL

LLLVVALGIGLFM Table 15

Rl 1 long spacer CAR: PJ_R1 l-CH2-CH3-41BB-Z-T2A-tEGFR (SEQ ID NO:43)

GTTAGACCAGATCTGAGCCTGGGAGCTCTCTGGCTAACTAGGGAACCCACTGCT TAAGCCTCAATAAAGCTTGCCTTGAGTGCTTCAAGTAGTGTGTGCCCGTCTGTTG TGTGACTCTGGTAACTAGAGATCCCTCAGACCCTTTTAGTCAGTGTGGAAAATC TCTAGCAGTGGCGCCCGAACAGGGACTTGAAAGCGAAAGGGAAACCAGAGGAG CTCTCTCGACGCAGGACTCGGCTTGCTGAAGCGCGCACGGCAAGAGGCGAGGG GCGGCGACTGGTGAGTACGCCAAAAATTTTGACTAGCGGAGGCTAGAAGGAGA GAGATGGGTGCGAGAGCGTCAGTATTAAGCGGGGGAGAATTAGATCGATGGGA AAAAATTCGGTTAAGGCCAGGGGGAAAGAAAAAATATAAATTAAAACATATAG TATGGGCAAGCAGGGAGCTAGAACGATTCGCAGTTAATCCTGGCCTGTTAGAAA CATCAGAAGGCTGTAGACAAATACTGGGACAGCTACAACCATCCCTTCAGACA GGATCAGAAGAACTTAGATCATTATATAATACAGTAGCAACCCTCTATTGTGTG CATCAAAGGATAGAGATAAAAGACACCAAGGAAGCTTTAGACAAGATAGAGGA AGAGCAAAACAAAAGTAAGAAAAAAGCACAGCAAGCAGCAGCTGACACAGGA CACAGCAATCAGGTCAGCCAAAATTACCCTATAGTGCAGAACATCCAGGGGCA AATGGTACATCAGGCCATATCACCTAGAACTTTAAATGCATGGGTAAAAGTAGT AG AAG AG A AGGCTTTCAG CCC AG AAGTGATACCCATGTTTTCAG C ATTATC AG A AGGAGCCACCCCACAAGATTTAAACACCATGCTAAACACAGTGGGGGGACATC AAGCAGCCATGCAAATGTTAAAAGAGACCATCAATGAGGAAGCTGCAGGCAAA GAGAAGAGTGGTGCAGAGAGAAAAAAGAGCAGTGGGAATAGGAGCTTTGTTCC TTGGGTTCTTGGGAGCAGCAGGAAGCACTATGGGCGCAGCGTCAATGACGCTG ACGGTACAGGCCAGACAATTATTGTCTGGTATAGTGCAGCAGCAGAACAATTTG CTGAGGGCTATTGAGGCGCAACAGCATCTGTTGCAACTCACAGTCTGGGGCATC AAGCAGCTCCAGGCAAGAATCCTCGCTGTGGAAAGATACCTAAAGGATCAACA GCTCCTGGGGATTTGGGGTTGCTCTGGAAAACTCATTTGCACCACTGCTGTGCCT TGGATCTACAAATGGCAGTATTCATCCACAATTTTAAAAGAAAAGGGGGGATTG GGGGGTACAGTGCAGGGGAAAGAATAGTAGACATAATAGCAACAGACATACAA ACTAAAGAATTACAAAAACAAATTACAAAAATTCAAAATTTTCGGGTTTATTAC AGGGACAGCAGAGATCCAGTTTGGGGATCAATTGCATGAAGAATCTGCTTAGG GTTAGGCGTTTTGCGCTGCTTCGCGAGGATCTGCGATCGCTCCGGTGCCCGTCA GTGGGCAGAGCGCACATCGCCCACAGTCCCCGAGAAGTTGGGGGGAGGGGTCG GCAATTGAACCGGTGCCTAGAGAAGGTGGCGCGGGGTAAACTGGGAAAGTGAT GTCGTGTACTGGCTCCGCCTTTTTCCCGAGGGTGGGGGAGAACCGTATATAAGT GCAGTAGTCGCCGTGAACGTTCTTTTTCGCAACGGGTTTGCCGCCAGAACACAG CTGAAGCTTCGAGGGGCTCGCATCTCTCCTTCACGCGCCCGCCGCCCTACCTGA GGCCGCCATCCACGCCGGTTGAGTCGCGTTCTGCCGCCTCCCGCCTGTGGTGCC TCCTGAACTGCGTCCGCCGTCTAGGTAAGT'l'TAAAGCTCAGGTCGAGACCGGGC CTTTGTCCGGCGCTCCCTTGGAGCCTACCTAGACTCAGCCGGCTCTCCACGCTTT GCCTGACCCTGCTTGCTCAACTCTACG'rCT TTG'I TCGTTTTCTGTTC TGCGCCGT TACAGATCCAAGCTGTGACCGGCGCCTACG GCTAGCj

scFv R12

GAATTCGCCACC[ATG|CTGCTGCTGGTGACAAGCCTGCTGCTGTGCGAGCTGCCC CACCCCGCCTTTCTGCTGATCCCCCAGAGCGTGAAAGAGTCCGAGGGCGACCTG GTCACACCAGCCGGCAACCTGACCCTGACCTGTACCGCCAGCGGCAGCGACATC

AACGACTACCCCATCTCTTGGGTCCGCCAGGCTCCTGGCAAGGGACTGGAATGG

ATCGGCT CATCAACAGCGGCGGCAGCACTTGGTACGCCAGCTGGGTCAAAGGC

CGGTTCACCATCAGCCGGACCAGCACCACCGTGGACCTGAAGATGACAAGCCT

GACCACCGACGACACCGCCACCTACTTTTGCGCCAGAGGCTACAGCACCTACTA

CGGCGACTTCAACATCTGGGGCCCTGGCACCCTGGTCACAATCTCTAGCGGCGG

AGGCGGCAGCGGAGGTGGAGGAAGTGGCGGCGGAGGATCCGAGCTGGTCATGA

CCCAGACCCCCAGCAGCACATCTGGCGCCGTGGGCGGCACCGTGACCATCAATT

GCCAGGCCAGCCAGAGCATCGACAGCAACCTGGCCTGGTTCCAGCAGAAGCCC

GGCCAGCCCCCCACCCTGCTGATCTACAGAGCCTCCAACCTGGCCAGCGGCGTG

CCAAGCAGATTCAGCGGCAGCAGATCTGGCACCGAGTACACCCTGACCATCTCC

GGCGTGCAGAGAGAGGACGCCGCTACCTATTACTGCCTGGGCGGCGTGGGCAA

CGTGTCCTACAGAACCAGCTTCGGCGGAGGTACTGAGGTGGTCGTCAAA

Ilinge/Spacer

Af CGGACCGjcCCTGCCCCCCTTGCCCT

CH2

GCCCCCGAGTTCCTGGGCG GACCCAG CGTGTTCCTGTTCCCCCCC A AGCCCAAG

GACACCCTGATGATCAGCCGGACCCCCGAGGTGACGTGCGTGGTGGTGGACGTG

AGCCAGGAAGATCCCGAGGTCCAGTTCAATTGGTACGTGGACGGCGTGGAAGT

GCACAACGCCAAGACCAAGCCCAGAGAGGAACAGTTCAACAGCACCTACCGGG

TGGTGTCTGTGCTGACCGTGCTGCACCAGGACTGGCTGAACGGCAAAGAATACA

AGTGCAAGGTGTCCAACAAGGGCCTGCCCAGCAGCATCGAAAAGACCATCAGC

AAGGCCAAG

CH3

GGCCAGCCTCGCGAGCCCCAGGTGTACACCCTGCCTCCCTCCCAGGAAGAGATG

ACCAAGAACCAGGTGTCCCTGACCTGCCTGGTGAAGGGCTTCTACCCCAGCGAC

ATCGCCGTGGAGTGGGAGAGCAACGGCCAGCCTGAGAACAACTACAAGACCAC

CCCTCCCGTGCTGGACAGCGACGGCAGCTTCTTCCTGTACAGCCGGCTGACCGT

GGACAAGAGCCGGTGGCAGGAAGGCAACGTCTTTAGCTGCAGCGTGATGCACG

AGGCCCTGCACAACCACTACACCCAGAAGAGCCTGAGCCTGTCCCTGGGCAAG

4-1 BB

ATGTTCTGGGTGCTGGTGGTGGTGGGCGGGGTGCTGGCCTGCTACAGCCTGCTG

GTGACAGTGGCCTTCATCATCTTTTGGGTGAAACGGGGCAGAAAGAAACTCCTG

TATATATTCAAACAACCATTTATGAGACCAGTACAAACTACTCAAGAGGAAGAT

GGCTGTAGCTGCCGATTTCCAGAAGAAGAAGAAGGAGGATGTGAACTG

CD3zeta

CGGGTGAAGTTCAGCAGAAGCGCCGACGCCCCTGCCTACCAGCAGGGCCAGAA

TCAGCTGTACAACGAGCTGAACCTGGGCAGAAGGGAAGAGTACGACGTCCTGG

ATAAGCGGAGAGGCCGGGACCCTGAGATGGGCGGCAAGCCTCGGCGGAAGAAC

CCCC GG AG G CCTGTATA AAAG AG ATGGCCGAGGCCT

CAGCGAGATCGGCATGAAGGGCGAGCGGAGGCGGGGCAAGGGCCACGACGGC

CTGTATCAGGGCCTGTCCACCGCCACCAAGGATACCTACGACGCCCTGCACATG

CAGGCCCTGCCCCCAAGG

T2A tEGFR ATGCTTCTCCTGGTGACAAGCCTTCTGCTCTGTGAGTTACCACACCCAGCATTCC TCCTGATCCCACGCAAAGTGTGTAACGGAATAGGTATTGGTGAATTTAAAGACT CACTCTCCATAAATGCTACGAATATTAAACACTTCAAAAACTGCACCTCCATCA GTGGCGATCTCCACATCCTGCCGGTGGCATTTAGGGGTGACTCCTTCACACATA CTCCTCCTCTli¥¾gliACAGGAACTGGATATTCTGAAAACCGTAAAGGAAATCA CAGGGTTTTTGCTGATTCAGGCTTGGCCTGAAAACAGGACGGACCTCCATGCCT TTGAGAACCTAGAAATCATACGCGGCAGGACCAAGCAACATGGTCAGTTTTCTC TTGCAGTCGTCAGCCTGAACATAACATCCTTGGGATTACGCTCCCTCAAGGAGA TAAGTGATGGAGATGTGATAATTTCAGGAAACAAAAATTTGTGCTATGCAAATA CAATAAACTGGAAAAAACTGTTTGGGACCTCCGGTCAGAAAACCAAAATTATA AGCAACAGAGGTGAAAACAGCTGCAAGGCCACAGGCCAGGTCTGCCATGCCTT GTGCTCCCCCGAGGGCTGCTGGGGCCCGGAGCCCAGGGACTGCGTCTCTTGCCG GAATGTCAGCCGAGGCAGGGAATGCGTGGACAAGTGCAACCTTCTGGAGGGTG AGCCAAGGGAGTTTGTGGAGAACTCTGAGTGCATACAGTGCCACCCAGAGTGC CTGCCTCAGGCCATGAACATCACCTGCACAGGACGGGGACCAGACAACTGTATC CAGTGTGCCCACTACATTGACGGCCCCCACTGCGTCAAGACCTGCCCGGCAGGA GTCATGGGAGAAAACAACACCCTGGTCTGGAAGTACGCAGACGCCGGCCATGT GTGCCACCTGTGCCATCCAAACTGCACCTACGGATGCACTGGGCCAGGTGTTGA AGGCTGTCCAACGAATGGGCCTAAGATCCCGTCCATCGCCACTGGGATGGTGGG GGCCCTCCTCTTGCTGCTGGTGGTGGCCCTGGGGATCGGCCTCTTCATGTGA

[GCGGCCGClTCTAGACCCGGGCTGCAGGAATTCGATATCAAGCTTATCGATAATC AACCTCTGGATTACAAAATTTGTGAAAGATTGACTGGTATTCTTAACTATGTTGC TCCTTTTACGCTATGTGGATACGCTGCTTTAATGCCTTTGTATCATGCTATTGCTT CCCGTATGGCTTTCATTTTCTCCTCCTTGTATAAATCCTGGTTGCTGTCTCTTTAT GAGGAGTTGTGGCCCGTTGTCAGGCAACGTGGCGTGGTGTGCACTGTGTTTGCT GACGCAACCCCCACTGGTTGGGGCATTGCCACCACCTGTCAGCTCCTTTCCGGG ACTTTCGCTTTCCCCCTCCCTATTGCCACGGCGGAACTCATCGCCGCCTGCCTTG CCCGCTGCTGGACAGGGGCTCGGCTGTTGGGCACTGACAATTCCGTGGTGTTGT CGGGGAAATCATCGTCCTTTCCTTGGCTGCTCGCCTGTGTTGCCACCTGGATTCT GCGCGGGACGTCCTTCTGCTACGTCCCTTCGGCCCTCAATCCAGCGGACCTTCCT TCCCGCGGCCTGCTGCCGGCTCTGCGGCCTCITCCGCGTCTTCGCCTTCGCCCTC AGACGAGTCGGATCTCCCTTTGGGCCGCCTCCCCGCATCGATACCGTCGACTAG CCGTACCTTTAAGACCAATGACTTACAAGGCAGCTGTAGATCTTAGCCACTTTTT AAAAGAAAAGGGGGGACTGGAAGGGCTAATTCACTCCCAAAGAAGACAAGATC TGCTTTTTGCCTGTACTGGGTCTCTCTGGTTAGACCAGATCTGAGCCTGGGAGCT CTCTGGCTAACTAGGGAACCCACTGCTTAAGCCTCAATAAAGCTTGCCTTGAGT GCTTCAAGTAGTGTGTGCCCGTCTGTTGTGTGACTCTGGTAACTAGAGATCCCTC AGACCCTTTTAGTCAGTGTGGAAAATCTCTAGCAGAATTCGATATCAAGCTTAT CGATACCGTCGACjCTCGAG|GGGGGGCCCGGTACCCAATTCGCCCTATAGTGAGT CGTATTACAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTG GCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAA TAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGG CGAATGGAAATTGTAAGCGTTAATATTTTGTTAAAATTCGCG ^r ITAAATTTTTGTT AAATCAGCTCATTrrTTAACCAATAGGCCGAAATCGGCAAAATCCCTTATAAAT CAAAAGAATAGACCGAGATAGGGTTGAGTGTTGTTCCAGTTTGGAACAAGAGT CCACTATTAAAGAACGTGGACTCCAACGTCAAAGGGCGAAAAACCGTCTATCA GGGCGATGGCCCACTACGTGAACCATCACCCTAATCAAGTTTTTTGGGGTCGAG GTGCCGTAAAGCACTAAATCGGAACCCTAAAGGGAGCCCCCGATTTAGAGCTTG ACGGGGAAAGCCGGCGAACGTGGCGAGAAAGGAAGGGAAGAAAGCGAAAGGA GCGGGCGCTAGGGCGCTGGCAAGTGTAGCGGTCACGCTGCGCGTAACCACCAC ACCCGCCGCGCTTAATGCGCCGCTACAGGGCGCGTCAGGTGGCACTTTTCGGGG AAATGTGCGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTAT CCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAG AGTATGAGTATTCAACAT TCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTG CCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGA TCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGAT CCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTTAAAGTT CTGCTATGTGGCGCGGTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGT CGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAA AAGCATCTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACC ATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACGATCGGAGGACCGAA GGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGATCG TTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGA TGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTA CTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAG GACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGG AGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAA GCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGA ACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACT GTCAGACCAAG'TTTACTCATATATACTTTAGATTGATTTAAAACTTCATTTTTAA TTTAAAAGGATCTAGGTGAAGATCCTTTTTGATAATCTCATGACCAAAATCCCTT AACGTGAGTTTI'CGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGAT CTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACC ACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCG AAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAG CCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCT CTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCG GGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACG GGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAG ATACCTACAGCGTGAGCTATGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGG CGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGA GCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTC TGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAA AACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTC ACATGTTCTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTT GAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGT GAGCGAGGAAGCGGAAGAGCGCCCAATACGCAAACCGCCTCTCCCCGCGCGTT GGCCGATTCATTAATGCAGCTGGCACGACAGGTTTCCCGACTGGAAAGCGGGCA GTGAGCGCAACGCAATTAATGTGAGTTAGCTCACTCATTAGGCACCCCAGGCTT TACACTT'PATGCTTCCGGCTCGTATGTTGTGTGGAATL'GTGAGCGGATAACAATT TCACACAGGAAACAGCTATGACCATGATTACGCCAAGCTCGAAATTAACCCTCA CTAAAGGGAACAAAAGCTGGAGCTCCACCGCGGTGGCGGC[CTCGAG|GTCGAGA TCCGGTCGACCAGCAACCATAGTCCCGCCCCTAACTCCGCCCATCCCGCCCCTA ACTCCGCCCAGTTCCGCCCATTCTCCGCCCCATGGCTGACTAATTTTTTTTATTTA TGCAGAGGCCGAGGCCGCCTCGGCCTCTGAGCTATTCCAGAAGTAGTGAGGAG GCTTTTTTGGAGGCCTAGGCTTTTGCAAAAAGCTTCGACGGTATCGATTGGCTCA TGTCCAACATTACCGCCATGTTGACATTGATTATTGACTAGTTATTAATAGTAAT CAATTACGGGGTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAAC TTACGGTAAATGGCCCGCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGT CAATAATGACGTATGTTCCCATAGTAACGCCAATAGGGACTTTCCATTGACGTC AATGGGTGGAGTATTTACGGTAAACTGCCCACTTGGCAGTACATCAAGTGTATC ATATGCCAAGTACGCCCCCTATTGACGTCAATGACGGTAAATGGCCCGCCTGGC ATTATGCCCAGTACATGACCTTATGGGACTTTCCTACTTGGCAGTACATCTACGT ATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACATCAATGGGCG TGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGTCAA TGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAACAA CTCCGCCCCATTGACGCAAATGGGCGGTAGGCGTGTACGGAATTCGGAGTGGCG AGCCCTCAGATCCTGCATATAAGGAGCTGCTTTTTGCCTGTACTGGGTCTCTCTG

Table 16

Leader JU 1- Hinge-CH2-CH3- CD28tm/41BB-Z-T2 A-tEGFR (SEQ ID NO:44)

Leader

M LLLVTSLLLCELPH PAFLLIP

Rl l scFv

QSV ESEGDLVTPAGTS1LTLTCTASGSD1NDYPISWVRQAPG GLEWIGFINSGGSTW YASWVKGRFTISRTSTTVDLKMTSLTTDDTATYFCARGYSTYYGDFNI GPGTLVT ISSGGGGSGGGGSGGGGSELV TQTPSSTSGAVGGTVTINCQASQSIDSNLAWFQQ KPGQPPTT.LIYRASNLASGVPSRFSGSRSGTEYTLTISGVQREDAATYYCLGGVGNV SYRTSFGGGTEVVVK

Hinge/Spacer

ESKYGPPCPPCP CH2

APEFLGGPSVFLFPP P DTLMiSRTPEVTgWVDVSQEDPEVQFNWYVDGVEVHN AKTKPREEQFNSTYRWS VLTVLHQD WLNGKEY @KVSN KGLPSSIEKTISKAK CH3

GQPREPQVYTLPPSQEEMTKNQVSLT^VKGFYPSDIAVEWESNGQPEN YKTTPP

VLDSDGSFFLYSRLTVDKSRWQEGNVFSgSVMHEALHNHYTQKSLSLSLGK

CD28tm

MFWVLVVVGGVLACYSLLVTVAFIIFWV

4-1 BB

KRGR KLLYIF QPFMRPVQTTQEEDGCSCRFPEEEEGGCEL CD3zeta

RV KFSRS ADAPAYQQGQN QL YN ELN LGRREEYDVLD RRGRDPEMGGKPRRI P QEGLYNELQKDKMAEAYSEIGMKGERRRGKGHDGLYQGLSTATKDTYDALHMQ ALPPR

T2A

LEG GG EGRG SLLTCGDVEENPGPR

tEGFR

MLLL VTS LLLCELPHPAFLLIPRKVCNG1GIGEF D S LS IN ATNI HFKNCTSI SGDLHI LPVAFRGDSFTHTPPLDPQELDILKTVKEITGFLLIQAWPEN RTDLHAFENLEIIRGRT KQHGQFSLAVVSLNITSLGLRSLKEISDGDVIISGNKNLCYANTINWKKLFGTSGQK TKIISNRGENSCKATGQVCHALCSPEGCWGPEPRDCVSCRNVSRGRECVDKCNLLE GEPREFVENSECIQCHPECLPQAMNITCTGRGPDNCIQCAHYIDGPI 1CV TCPAGVM GENNTLVWKYADAGHVCHLCHPMCTYGCTGPGLEGCPTNGPKIPSIATGMVGALL LLLVVALGIGLFM Table 17

Rl 1 intermediate spacer CAR: PJ Rl l-CH3-41BB-Z-T2A-tEGFR (SEQ ID NO:45)

GTTAGACCAGATCTGAGCCTGGGAGCTCTCTGGCTAACTAGGGAACCCACTGCT TAAGCCTCAATAAAGCTTGCCTTGAGTGCTTCAAGTAGTGTGTGCCCGTCTGTTG TGTGACTCTGGTAACTAGAGATCCCTCAGACCCTTTTAGTCAGTGTGGAAAATC TCTAGCAGTGGCGCCCGAACAGGGACTTGAAAGCGAAAGGGAAACCAGAGGAG CTCTCTCGACGCAGGACTCGGCTTGCTGAAGCGCGCACGGCAAGAGGCGAGGG GCGGCGACTGGTGAGTACGCCAAAAATTTTGACTAGCGGAGGCTAGAAGGAGA GAGATGGGTGCGAGAGCGTCAGTATTAAGCGGGGGAGAATTAGATCGATGGGA AAAAATTCGGT AAGGCCAGGGGGAAAGAAAAAATATAAATTAAAACATATAG TATGGGCAAGCAGGGAGCTAGAACGATTCGCAGTTAATCCTGGCCTGTTAGAAA CATCAGAAGGCTGTAGACAAATACTGGGACAGCTACAACCATCCCTTCAGACA GGATCAGAAGAACTTAGATCATTATATAATACAGTAGCAACCCTCTATTGTGTG CATCAAAGGATAGAGATAAAAGACACCAAGGAAGCTTTAGACAAGATAGAGGA AGAGCAAAACAAAAGTAAGAAAAAAGCACAGCAAGCAGCAGCTGACACAGGA CACAGCAATCAGGTCAGCCAAAATTACCCTATAGTGCAGAACATCCAGGGGCA AATGGTACATCAGGCCATATCACCTAGAACTTTAAATGCATGGGTAAAAGTAGT AGAAGAGAAGGCTTTCAGCCCAGAAGTGATACCCATGTTTTCAGCATTATCAGA AGGAGCCACCCCACAAGATTTAAACACCATGCTAAACACAGTGGGGGGACATC AAGCAGCCATGCAAATGTTAAAAGAGACCATCAATGAGGAAGCTGCAGGCAAA GAGAAGAGTGGTGCAGAGAGAAAAAAGAGCAGTGGGAATAGGAGCTTTGTTCC TTGGGTTCTTGGGAGCAGCAGGAAGCACTATGGGCGCAGCGTCAATGACGCTG ACGGTACAGGCCAGACAATTATTGTCTGGTATAGTGCAGCAGCAGAACAATTTG CTGAGGGCTATTGAGGCGCAACAGCATCTGTTGCAACTCACAGTCTGGGGCATC AAGCAGCTCCAGGCAAGAATCCTGGCTGTGGAAAGATACCTAAAGGATCAACA GCTCCTGGGGATTTGGGGTTGCTCTGGAAAACTCATTTGCACCACTGCTGTGCCT TGGATCTACAAATGGCAGTATTCATCCACAATTTTAAAAGAAAAGGGGGGATTG GGGGGTACAGTGCAGGGGAAAGAATAGTAGACATAATAGCAACAGACATACAA ACTAAAGAATTACAAAAACAAATTACAAAAATTCAAAATTTTCGGGTTTATTAC AG G GAC AGCAG AG ATCC AGTTTGG G GATC A ATTGC ATG AAG AATCTGCTT AGG GTTAG GCGTTTTG CGCTGCTTCG CG AGG ATCTGCGATCGCTCCG GTGCCCGTCA GTGGGCAGAGCGCACATCGCCCACAGTCCCCGAGAAGTTGGGGGGAGGGGTCG GCAATTGAACCGGTGCCTAGAGAAGGTGGCGCGGGGTAAACTGGGAAAGTGAT GTCGTGTACTGGCTCCGCCTTTTTCCCGAGGGTGGGGGAGAACCGTATATAAGT GCAGTAGTCGCCGTGAACGTTCTTTTTCGCAACGGGTTTGCCGCCAGAACACAG CTGAAGCTTCGAGGGGCTCGCATCTCTCCTTCACGCGCCCGCCGCCCTACCTGA GGCCGCC ATCC ACGC CGGTTG AGTCG CGTTCTGCCGCCTCCC GCCTGTGGTGCC TCCTGAACTGCGTCCGCCGTCTAGGTAAGTTTAAAGCTCAGGTCGAGACCGGGC CTTTGTCCGGCGCTCCCTTGGAGCCTACCTAGACTCAGCCGGCTCTCCACGCTTT GCCTGACCCTGCTTGCTCAACTCTACGTCTTTGTTTCGTTTTCTGTTCTGCGCCGT TACAGATCCAAGCTGTGACCGGCGCCTACG

GCTAGQ

Rl l scFV

GAATTCGCCACC|ATG|CTGCTGCTGGTGACAAGCCTGCTGCTGTGCGAGCTGCCC CACCCCGCC'ETTCTGCTGATCCCCCAGAGCGTGAAAGAGTCCGAGGGCGACCTG GTCACACCAGCCGGCAACCTGACCCTGACCTGTACCGCCAGCGGCAGCGACATC AACGACTACCCCATCTCTTG GGTCCGCC AGGCTCCTGGCAAG G G ACTGGA ATGG ATCGGCTTCATCAACAGCGGCGGCAGCACTTGGTACGCCAGCTGGGTCAAAGGC CGGTTCACCATCAGCCGGACCAGCACCACCGTGGACCTGAAGATGACAAGCCT GACCACCGACGACACCGCCACCTACTTTTGCGCCAGAGGCTACAGCACCTACTA CGGCGACTTCAACATCTGGGGCCCTGGCACCCTGGTCACAATCTCTAGCGGCGG AGGCGGCAGCGGAGGTGGAGGAAGTGGCGGCGGAGGATCCGAGCTGGTCATGA CCCAGACCCCCAGCAGCACATCTGGCGCCGTGGGCGGCACCGTGACCATCAATT GCCAGGCCAGCCAGAGCATCGACAGCAACCTGGCCTGGTTCCAGCAGAAGCCC GGCCAGCCCCCCACCCTGCTGATCTACAGAGCCTCCAACCTGGCCAGCGGCGTG CCAAGCAGATTCAGCGGCAGCAGATCTGGCACCGAGTACACCCTGACCATCTCC GGCGTGCAGAGAGAGGACGCCGCTACCTATTACTGCCTGGGCGGCGTGGGCAA CGTGTCCTACAGAACCAGCTTCGGCGGAGGTACTGAGGTGGTCGTCAAA

Hinge/spacer

f Aku; A(X x i|cx ' ! (!('( x x x X. I ( i( ( '( r I

GCCCCCG AGTTCCTGGGCG GACCC AGCG TGTTCCTGTTCCCCCCC A AGCCC AAG GACACCCTGATGATCAGCCGGACCCCCGAGGTGACCTGCGTGGTGGTGGACGTG AGCCAGGAAGATCCCGAGGTCCAGTTCAATTGGTACGTGGACGGCGTGGAAGT GCACAACGCCAAGACCAAGCCCAGAGAGGAACAGTTCAACAGCACCTACCGGG TGGTGTCTGTGCTGACCGTGCTGCACCAGGACTGGCTGAACGGCAAAGAATACA AGTGCAAGGTGTCCAACAAGGGCCTGCCCAGCAGCATCGAAAAGACCATCAGC AAGGCCAAG CH3

GGCCAGCCTCGCGAGCCCCAGGTGTACACCCTGCCTCCCTCCCAGGAAGAGATG ACCAAGAACCAGGTGTCCCTGACCTGCCTGGTGAAGGGCTTCTACCCCAGCGAC ATCGCCGTGGAGTGGGAGAGCAACGGCCAGCCTGAGAACAACTACAAGACCAC CCCTCCCGTGCTGGACAGCGACGGCAGCTTCTTCCTGTACAGCCGGCTGACCGT GG AC A AG AGCCGGTGG C AGG AAG G CA ACGTCTTTAGCTGCAGCGTGATGC ACG AG GCCCTGCACAACCACTAC ACCC AG AAG AGCCTG AG CCTGTCCCTGG GC A AG

4-1 BB

ATGTTCTGGGTGCTGGTGGTGGTGGGCGGGGTGCTGGCCTGCTACAGCCTGCTG G TG ACAG TGG CCTTCATCATCTTTTGGGTG AAACGGGG CAG AAAG A A ACTCCTG TATATATTCAAACAACCATTTATGAGACCAGTACAAACTACTCAAGAGGAAGAT GGCTGTAGCTGCCGATTTCCAGAAGAAGAAGAAGGAGGATGTGAACTG CD3zeta

CGGGTGAAGTTCAGCAGAAGCGCCGACGCCCCTGCCTACCAGCAGGGCCAGAA TCAGCTGTACAACGAGCTGAACCTGGGCAGAAGGGAAGAGTACGACGTCCTGG ATAAGCGGAGAGGCCGGGACCCTGAGATGGGCGGCAAGCCTCGGCGGAAGAAC CCCCAGGAAGGCCTGTA'rAACGAACTGCAGAAAGACAAGATGGCCGAGGCCTA CAGCGAGATCGGCATGAAGGGCGAGCGGAGGCGGGGCAAGGGCCACGACGGC CTGTATCAGGGCCTGTCCACCGCCACCAAGGATACCTACGACGCCCTGCACATG CAGGCCCTGCCCCCAAGG

T2A

CTCGAGGGCGGCGGAGAGGGCAGAGGAAGTCTTCTAACATGCGGTGACGTGGA

GGAGAATCCCGGCCC TAGG tEGFR

ATGCTTCTCCTGGTGACAAGCCTTCTGCTCTGTGAGTTACCACACCCAGCATTCC TCCTGATCCCACGCAAAGTGTGTAACGGAATAGGTATTGGTGAATTTAAAGACT CACTCTCCATAAATGCTACGAATATTAAACACTTCAAAAACTGCACCTCCATCA GTGGCGATCTCCACATCCTGCCGGTGGCATTTAGGGGTGACTCCTTCACACATA CTCCTCCTCTl^^^gACAGGAACTGGATATTCTGAAAACCGTAAAGGAAATCA CAGGGTTTTTGCTGATTCAGGCTTGGCCTGAAAACAGGACGGACCTCCATGCCT TTGAGAACCTAGAAATCATACGCGGCAGGACCAAGCAACATGGTCAGTTTTCTC TTGCAGTCGTCAGCCTGAACATAACATCCTTGGGATTACGCTCCCTCAAGGAGA TAAGTGATGGAGATGTGATAATTTCAGGAAACAAAAATTTGTGCTATGCAAATA CAATAAACTGGAAAAAACTGTTTGGGACCTCCGGTCAGAAAACCAAAATTATA AGCAACAGAGGTGAAAACAGCTGCAAGGCCACAGGCCAGGTCTGCCATGCCTT GTGCTCCCCCGAGGGCTGCTGGGGCCCGGAGCCCAGGGACTGCGTCTCTTGCCG GAATGTCAGCCGAGGCAGGGAATGCGTGGACAAGTGCAACCTTCTGGAGGGTG AGCCAAGGGAGTTTGTGGAGAACTCTGAGTGCATACAGTGCCACCCAGAGTGC CTGCCTCAGGCCATGAACATCACCTGCACAGGACGGGGACCAGACAACTGTATC CAGTGTGCCCACTACATTGACGGCCCCCACTGCGTCAAGACCTGCCCGGCAGGA GTCATGGGAGAAAACAACACCCTGGTCTGGAAGTACGCAGACGCCGGCCATGT GTGCCACCTGTGCCATCCAAACTGCACCTACGGATGCACTGGGCCAGGTCTTGA AGGCTGTCCAACGAATGGGCCTAAGATCCCGTCCATCGCCACTGGGATGGTGGG GGCCCTCCTCTTGCTGCTGGTGGTGGCCCTGGGGATCGGCCTCTTCATGI ^' GA

GCGGCCGCfrCTAGACCCGGGCTGCAGGAATTCGATATCAAGCTTATCGATAATC

AACCTCTGGATTACAAAATTTGTGAAAGATTGACTGGTATTCTTAACTATGTTGC TCCTTTTACGCTATGTGGATACGCTGCTTTAATGCCTTTGTATCATGCTATTGCTT CCCGTATGGCTTTCATTTTCTCCTCCTTGTATAAATCCTGGTTGCTGTCTCTTTAT GAGGAGTTGTGGCCCGTTGTCAGGCAACGTGGCGTGGTGTGCACTGTGTTTGCT GACGCAACCCCCACTGGTTGGGGCATTGCCACCACCTGTCAGCTCCTTTCCGGG ACTTTCGCTTTCCCCCTCCCTATTGCCACGGCGGAACTCATCGCCGCCTGCCTTG CCCGCTGCTGGACAGGGGCTCGGCTGTTGGGCACTGACAATTCCGTGGTGTTGT CGGGGAAATCATCGTCCTTTCCTTGGCTGCTCGCCTGTGTTGCCACCTGGATTCT GCGCGGGACGTCCTTCTGCTACGTCCCTTCGGCCCTCAATCCAGCGGACCTTCCT TCCCGCGGCCTGCTGCCGGCTCTGCGGCCTCTTCCGCGTCTTCGCCTTCGCCCTC AGACGAGTCGGATCTCCCTTTGGGCCGCCTCCCCGCATCGATACCGTCGACTAG CCGTACCTTTAAGACCAATGACTTACAAGGCAGCTGTAGATCTTAGCCACTTTTT AAAAGAAAAGGGGGGACTGGAAGGGCTAATTCACTCCCAAAGAAGACAAGATC TGCTTTTTGCCTGTACTGGGTCTCTCTGGTTAGACCAGATCTGAGCCTGGGAGCT CTCTGGCTAACTAGGGAACCCACTGCTTAAGCCTCAATAAAGCTTGCCTTGAGT GCTTCAAGTAGTGTGTGCCCGTCTGTTGTGTGACTCTGGTAAC'I AGAGATCCCTC AGACCCTTTTAGTCAGTGTGGAAAATCTCTAGCAGAATTCGATATCAAGCTTAT CGATACCGTCGACjCTCGAGlGGGGGGCCCGGTACCCAATTCGCCCTATAGTGAGT CGTATTACAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTG GCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAA TAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTIOCGCAGCCTGAATGG CGAATGGAAATTGTAAGCGTTAATATTTTGTTAAAATTCGCG'ITAAATTTTTGTT AAATCAGCTCATTTTTTAACCAATAGGCCGAAATCGGCAAAATCCCTTATAAAT CAAAAGAATAGACCGAGATAGGGTTGAGTGTTGTTCCAGTTTGGAACAAGAGT CCACTATTAAAGAACGTGGACTCCAACGTCAAAGGGCGAAAAACCGTCTATCA GGGCGATGGCCCACTACGTGAACCATCACCCTAATCAAGTTTTTTGGGGTCGAG GTGCCGTAAAGCACTAAATCGGAACCCTAAAGGGAGCCCCCGATTTAGAGCTTG ACGGGGAAAGCCGGCGAACGTGGCGAGAAAGGAAGGGAAGAAAGCGAAAGGA GCGGGCGCTAGGGCGCTGGCAAGTGTAGCGGTCACGCTGCGCGTAACCACCAC ACCCGCCGCGCTTAATGCGCCGCTACAGGGCGCGTCAGGTGGCACTTTTCGGGG AAATGTGCGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTAT CCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAG AGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTG CCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGA TCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGAT CCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTTAAAGTT CTGCTATGTGGCGCGGTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGT CGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAA AAGCATCTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACC ATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACGATCGGAGGACCGAA GGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGATCG TTG GGAACCGGAGCTGA ATGA AGCC A ACCA AACGACG AG CGTGACACCACG A TGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTrA CTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAG GACCACTTCTGCGCTCGGCCCTTCCG G CTGGCTGGTTTATTGCTG ATAAATCTGG AGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAA GCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGA ACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACT GTCAGACCAAGTTTACTCATATATACTTTAGATTGATTTAAAACTTCATTTTTAA TTTAAAAGGATCTAGGTGAAGATCCTTTTTGATAATCTCATGACCAAAATCCCTT AACGTGAGTTTTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGAT CTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACC ACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCG AAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAG CCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCT CTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCG GGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACG GGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAG ATACCTAC AGCGTGAGCT ATG AG A A AGCGCC ACGCTTCCCGA AG G GAGA AAG G CGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGA GCTTCC AGGGG GAAACGCCTGGTATCTTTATAGTCCTGTCG G GTTTCG CC ACCTC TGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAA AACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTC ACATGTTCTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTT GAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGT GAGCGAGGAAGCGGAAGAGCGCCCAATACGCAAACCGCCTCTCCCCGCGCGTT GG CCGATTCATTA ATGC AGCTGGCACG AC AG G TTTCCCG ACTG G AAAGCGG GC A GTGAGCGCAACGCAATTAATGTGAGTTAGCTCACTCATTAGGCACCCCAGGCTT TACACTTTATGCTTCCGGCTCGTATGTTGTGTGGAATTGTGAGCGGATAACAATT TCAC ACAG GA AACA GCTATG ACC ATG ATTACGCC A AG CTCGAAATTAACCCTC A

CTAAAGGGAACAAAAGCTGGAGCTCCACCGCGGTGGCGGC|CTCGAG|GTCGAGA TCCGGTCGACCAGCAACCATAGTCCCGCCCCTAACTCCGCCCATCCCGCCCCTA ACTCCGCCCAGTTCCGCCCATTCTCCGCCCCATGGCTGACTAATTTTTTTTATTTA TGCAGAGGCCGAGGCCGCCTCGGCCTCTGAGCTATTCCAGAAGTAGTGAGGAG GCTTTTTTGGAGGCCTAGGCTTTTGCAAAAAGCTTCGACGGTATCGATTGGCTCA TGTCCAACATTACCGCCATGTTGACATTGATTATTGACTAGTTATTAATAGTAAT CAATTACGGGGTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAAC TTACGGTAAATGGCCCGCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGT CAATAATGACGTATGTTCCCATAGTAACGCCAATAGGGACTTTCCATTGACGTC AATGGGTGGAGTATTTACGGTAAACTGCCCACTTGGCAGTACATCAAGTGTATC ATATGCCAAGTACGCCCCCTATTGACGTCAATGACGGTAAATGGCCCGCCTGGC ATTATGCCCAGTACATGACCTTATGGGACTTTCCTACTTGGCAGTACATCTACGT ATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACATCAATGGGCG TGGATAGCGGT TGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGTCAA TGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAACAA CTCCGCCCCATTGACGCAAATGGGCGGTAGGCGTGTACGGAATTCGGAGTGGCG AGCCCTCAGATCCTGCATATAAGCAGCTGCTTTTTGCCTGTACTGGGTCTCTCTG

Table 18

Leader Rl 1- Hinge-CH3- CD28tm/41 BB-Z-T2 A-tEGFR (SEQ ID NO:46)

Leader

MLLLVTSLLLCELPHPAFLLLP

scFV Rll

QSVKESEGDLVTPAGNLTLTCTASGSDI 3YPISWVRQAPGKGLE IGFINSGGSTW YA S W VKGRFT1SRTSTTVDLKMTSLTTDDT ATYFC ARGYSTYYGDFNI WGPGTLVT ISSGGGGSGGGGSGGGGSELVMTQTPSSTSGAVGGTVTINCQASQSIDSNLAWFQQ KPGQPPTLLIYRAS LASGVPSRFSGSRSGTEYTLTISGVQREDAATYYCLGGVGNV SYRTSFGGGTEVWK

Hinge/spacer

ESKYGPPCPPCP CH3

GQPREPQV YTLPPSQEEMTKN Q VSLTJC|LV GF YPS D] A V E W ESNGQPEN N Y TTPP VLDSDGSFFLYSRLTVDKSRWQEGNVFSgSVMHEALHNHYTQKSLSLSLGK CD28tm

MFWVLVVVGGVLACYSLLVTVAFIIFWV

4-1 BB

KJR.GRK LLYIFKQPFMRPVQTTQEEDGCSCRFPEEEEGGCEL

CD3zeta

RV FSRSADAPAYQQGQMQLYNELNLGRREEYDVLD RRGRDPEMGGKPRRI IMP

QEGLY ELQK MAEAYSEIGMKGERRRG GHDGLYQGLSTATKDTYDALHMQ

ALPPR

T2A

LEGGGEGRGSLLTCGDVEEN PGPRM

tEGFR

LLLVTSLLLCELPHPAFLLIPR VCNGIGIGEF DSLSINATNI HF NCTSISGDLHIL PVAFRGDSFTHTPPLDPQELDILKTV EITGFLLIQAWPENRTDLHAFEMLEIIRGRT QHGQFSLAVVSLNITSLGLRSL EISDGDVJISGNICNLCYANTINW I LFGTSGQKT IISNRGENSCKATGQVCHALCSPEGCWGPEPRDCVSCRNVSRGRECVDKCNLLEG EPREFVENSECIQCHPECLPQAMNITCTGRGPDNCIQCAHYIDGPHCVI TCPAGVMG E NTLVWKYADAGHVCHLCHPNCTYGCTGPGLEGCPTNGP IPSIATGMVGALLL LLVVALG1GLFM Table 19

Rl 1 short spacer CAR: PJ Rl 1- 41 BB-Z-T2A-tEGFR(SEQ ID NO:47)

GTTAGACCAGATCTGAGCCTGGGAGCTCTCTGGCTAACTAGGGAACCCACTGCT TAAGCCTCAATAAAGCTTGCCTTGAGTGCTTCAAGTAGTGTGTGCCCGTCTGTTG TGTGACTCTGGTAACTAGAGATCCCTCAGACCCTTTTAGTCAGTGTGGAAAATC TCTAGCAGTGGCGCCCGAACAGGGACTTGAAAGCGAAAGGGAAACCAGAGGAG CTCTCTCGACGCAGGACTCGGCTTGCTGAAGCGCGCACGGCAAGAGGCGAGGG GCGGCGACTGGTGAGTACGCCAAAAATTTTGACTAGCGGAGGCTAGAAGGAGA GAGATGGGTGCGAGAGCGTCAGTATTAAGCGGGGGAGAATTAGATCGATGGGA AAAAATTCGGTTAAGGCCAGGGGGAAAGAAAAAATATAAATTAAAACATATAG TATGGG C AAG CAGGG AGCTAGAACGATTCGCAGTTAATCCTGGCCTGTTAGAAA CATCAGAAGGCTGTAGACAAATACTGGGACAGCTACAACCATCCCTTCAGACA GGATCAGAAGAACTTAGATCATTATATAATACAGTAGCAACCCTCTATTGTGTG CATCAAAGGATAGAGATAAAAGACACCAAGGAAGCTTTAGACAAGATAGAGGA AGAGCAAAACAAAAGTAAGAAAAAAGCACAGCAAGCAGCAGCTGACACAGGA CACAGCAATCAGGTCAGCCAAAATTACCCTATAGTGCAGAACATCCAGGGGCA AATGGTACATCAGGCCATATCACCTAGAACTTTAAATGCATGGGTAAAAGTAGT AGAAGAGAAGGCTTTCAGCCCAGAAGTGATACCCATGTTTTCAGCATTATCAGA AGGAGCCACCCCACAAGATTTAAACACCATGCTAAACACAGTGGGGGGACATC AAGCAGCCATGCAAATGTTAAAAGAGACCATCAATGAGGAAGCTGCAGGCAAA GAGAAGAGTGGTGCAGAGAGAAAAAAGAGCAGTGGGAATAGGAGCTTTGTTCC TTGGGTTCTTGGGAGCAGCAGGAAGCACTATGGGCGCAGCGTCAATGACGCTG ACGGTACAGGCCAGACAATTATTGTCTGGTATAGTGCAGCAGCAGAACAATTTG CTGAGGGCTATTGAGGCGCAACAGCATCTGTTGCAACTCACAGTCTGGGGCATC AAGCAGCTCCAGGCAAGAATCCTGGCTGTGGAAAGATACCTAAAGGATCAACA GCTCCTGGGGATTTGGGGTTGCTCTGGAAAACTCATTTGCACCACTGCTGTGCCT TGGATCTACAAATGGCAGTATTCATCCACAATTT AAAAGAAAAGGGGGGATTG GGGGGTACAGTGCAGGGGAAAGAATAGTAGACATAATAGCAACAGACATACAA ACTAAAGAATTACAAAAACAAATTACAAAAATTCAAAATTTTCGGGTTTATTAC AGGGACAGCAGAGATCCAGTTTGGGGATCAATTGCATGAAGAATCTGCTTAGG GTTAGG CGTTTTG CGCTGCTTCG CGAG GATCTGCGATCGCTCCG GTGCCCG TCA GTGGGCAGAGCGCACATCGCCCACAGTCCCCGAGAAGTTGGGGGGAGGGGTCG GCAATTGAACCGGTGCCTAGAGAAGGTGGCGCGGGGTAAACTGGGAAAGTGAT GTCGTGTACTGGCTCCGCCTTTTTCCCGAGGGTGGGGGAGAACCGTATATAAGT GCAGTAGTCGCCGTGAACGTTCTTTTTCGCAACGGGTTTGCCGCCAGAACACAG CTGAAGCTTCGAGGGGCTCGCATCTCTCCTTCACGCGCCCGCCGCCCTACCTGA GGCCGCCATCCACGCCGGTTGAGTCGCGTTCTGCCGCCTCCCGCC1 GTGGTGCC TCCTGAACTGCGTCCGCCGTCTAGGTAAGTTTAAAGCTCAGGTCGAGACCGGGC CTTTGTCCGGCGCTCCCTTGGAGCCTACCTAGACTCAGCCGGCTCTCCACGCTTT GCCTGACCCTGCTTGCTCAACTCTACGTCTTTGTTTCGTTTTCTGTTCTGCGCCGT TACAGATCCAAGCTGTGACCGGCGCCTACG

kjCTAGC

scFV Rl 1

GAA'rrCGCCACqATG|CTGCTGCTGGTGACAAGCCTGCTGCTGTGCGAGCTGCCC CACCCCGCCTTTCTGCTGATCCCCCAGAGCGTGAAAGAGTCCGAGGGCGACCTG GTCACACCAGCCGGCAACCTGACCCTGACCTGTACCGCCAGCGGCAGCGACATC AACGACTACCCCATCTCTTGGGTCCGCCAGGCTCCTGGCAAGGGACTGGAATGG ATCGGCTTCATCAACAGCGGCGGCAGCACTTGGTACGCCAGCTGGGTCAAAGGC CGGTTCACCATCAGCCGGACCAGCACCACCGTGGACCTGAAGATGACAAGCCT GACCACCGACGACACCGCCACCTACTTTTGCGCCAGAGGCTACAGCACCTACTA CGGCGACTTCAACATCTGGGGCCCTGGCACCCTGGTCACAATCTCTAGCGGCGG AGGCGGCAGCGGAGGTGGAGGAAGTGGCGGCGGAGGATCCGAGCTGGTCATGA CCCAGACCCCCAGCAGCACATCTGGCGCCGTGGGCGGCACCGTGACCATCAATT GCCAGGCCAGCCAGAGCATCGACAGCAACCTGGCCTGGTTCCAGCAGAAGCCC GGCCAGCCCCCCACCCTGCTGATCTACAGAGCCTCCAACCTGGCCAGCGGCGTG CCAAGCAGATTCAGCGGCAGCAGATCTGGCACCGAGTACACCCTGACCATCTCC GGCGTGCAGAGAGAGGACGCCGCTACCTATTACTGCCTGGGCGGCGTGGGCAA CGTGTCCTACAGAACCAGCTTCGGCGGAGGTACTGAGGTGGTCGTCAAA

Hinge/spacer

GGCCAGCCTCGCGAGCCCCAGGTGTACACCCTGCCTCCCTCCCAGGAAGAGATG ACCAAGAACCAGGTGTCCCTGACCTGCCTGGTGAAGGGCTTCTACCCCAGCGAC ATCGCCGTGGAGTGGGAGAGCAACGGCCAGCCTGAGAACAACTACAAGACCAC CCCTCCCGTGCTGGACAGCGACGGCAGCTTCTTCCTGTACAGCCGGCTGACCGT GGACAAGAGCCGGTGGCAGGAAGGCAACGTCTTTAGCTGCAGCGTGATGCACG AGGCCCTGCACAACCACTACACCCAGAAGAGCCTGAGCCTGTCCCTGGGCAAG

4-1 BB

ATGTTCTGGGTGCTGGTGGTGGTGGGCGGGGTGCTGGCCTGCTACAGCCTGCTG GTGACAGTGGCCTTCATCATCTTTTGGGTGAAACGGGGCAGAAAGAAACTCCTG TATATATTCAAACAACCATTTATGAGACCAGTACAAACTACTCAAGAGGAAGAT GGCTGTAGCTGCCGATTTCCAGAAGAAGAAGAAGGAGGATGTGAACTG CD3zeta

CGGGTGAAGTTCAGCAGAAGCGCCGACGCCCCTGCCTACCAGCAGGGCCAGAA TCAGCTGTACAACGAGCTGAACCTGGGCAGAAGGGAAGAGTACGACGTCCTGG ATAAGCGGAGAGGCCGGGACCCTGAGATGGGCGGCAAGCCTCGGCGGAAGAAC CCCCAGGAAGGCCTGTATAACGAACTGCAGAAAGACAAGATGGCCGAGGCCTA CAGCGAGATCGGCATGAAGGGCGAGCGGAGGCGGGGCAAGGGCCACGACGGC CTGTATCAGGGCCTGTCCACCGCCACCAAGGATACCTACGACGCCCTGCACATG CAGGCCCTGCCCCCAAGG

T2A

|C ^r rCGAG|GGCGGCGGAGAGGGCAGAGGAAGTCTTCTAACATGCGGTGACGTGGA

GGAGAATCCCGGCCCTAGG

tEGFR

ATGCTTCTCCTGGTGACAAGCCTTCTGCTCTGTGAGTTACCACACCCAGCATTCC TCCTGATCCCACGCAAAGTGTGTAACGGAATAGGTATTGGTGAATTTAAAGACT CACTCTCCATAAATGCTACGAATATTAAACACTTCAAAAACTGCACCTCCATCA GTGG CG ATCTCC ACATCCTG CCGGTGGCATTTAG GGGTG ACTCCTTC AC AC ATA

CTCCTCCTCT|GGATCG|ACAGGAACTGGATATTCTGAAAACCGTAAAGGAAATCA CAGGGTTTTTGCTGATTCAGGCTTGGCCTGAAAACAGGACGGACCTCCATGCCT TTGAGAACCTAGAAATCATACGCGGCAGGACCAAGCAACATGGTCAGTTTTCTC TTGCAGTCGTCAGCCTGAACATAACATCCTTGGGATTACGCTCCCTCAAGGAGA TAAGTGATGGAGATGTGATAATTTCAGGAAACAAAAATTTGTGCTATGCAAATA C AATA AACTGGAAAAAACTGTTTG GGACCTCCG GTCAGA AAACCAAAATTATA AGCAACAGAGGTGAAAACAGCTGCAAGGCCACAGGCCAGGTCTGCCATGCCTT GTGCTCCCCCGAGGGCTGCTGGGGCCCGGAGCCCAGGGACTGCGTCTCTTGCCG GAATGTCAGCCGAGGCAGGGAATGCGTGGACAAGTGCAACCTTCTGGAGGGTG AGCCAAGGGAGTTTGTGGAGAACTCTGAGTGCATACAGTGCCACCCAGAGTGC CTGCCTCAGGCCATGAACATCACCTGCACAGGACGGGGACCAGACAACTGTATC CAGTGTGCCCACTACATTGACGGCCCCCACTGCGTCAAGACCTGCCCGGCAGGA GTCATGGGAGAAAACAACACCCTGGTCTGGAAGTACGCAGACGCCGGCCATGT GTGCCACCTGTGCCATCCAAACTGCACCTACGGATGCACTGGGCCAGGTCTTGA AGGCTGTCCAACGAATGGGCCTAAGATCCCGTCCATCGCCACTGGGATGGTGGG GGCCCTCCTCTTGCTGCTGGTGGTGGCCCTGGGGATCGGCCTCTTCATGTGl

GCGGCCGCfTCTAGACCCGGGCTGCAGGAATTCGATATCAAGCTTATCGATAATC AACCTCTGGATTACAAAATTTGTGAAAGATTGACTGGTATTCTTAACTATGTTGC TCCTTTTACGCTATGTGGATACGCTGCTTTAATGCCTTTGTATCATGCTATTGCTT CCCGTATGGCTTTCATTTTCTCCTCCTTGTATAAATCCTGGTTGCTGTCTCTTTAT GAGGAGTTGTGGCCCGTTGTCAGGCAACGTGGCGTGGTGTGCACTGTGTTTGCT GACGCAACCCCCACTGGTTGGGGCATTGCCACCACCTGTCAGCTCCTTTCCGGG ACTTTCGCTTTCCCCCTCCCTATTGCCACGGCGGAACTCATCGCCGCCTGCCTTG CCCGCTGCTGGACAGGGGCTCGGCTGTTGGGCACTGACAATTCCGTGGTGTTGT CGGGGAAATCATCGTCCTTTCCTTGGCTGCTCGCCTGTGTTGCCACCTGGATTCT GCGCGGGACGTCCT CTGCTACGTCCCTTCGGCCCTCAATCCAGCGGACCTTCCT TCCCGCGGCCTGCTGCCGGCTCTGCGGCCTCTTCCGCGTCTTCGCCTTCGCCCTC AGACGAGTCGGATCTCCCTTTGGGCCGCCTCCCCGCATCGATACCGTCGACTAG CCGTACCTTTAAGACCAATGACTTACAAGGCAGCTGTAGATCTTAGCCACTTTTT AAAAGAAAAGGGGGGACTGGAAGGGCTAATTCACTCCCAAAGAAGACAAGATC TGCTTTTTGCCTGTACTGGGTCTCTCTGGTTAGACCAGATCTGAGCCTGGGAGCT CTCTGGCTAACTAGGGAACCCACTGCTTAAGCCTCAATAAAGCTTGCCTl'GAGT GCTTCAAGTAGTGTGTGCCCGTCTGTTGTGTGACTCTGGTAACTAGAGATCCCTC AGACCCTTTTAGTCAGTGTGGAAAATCTCTAGCAGAATTCGATATCAAGCTTAT CGATACCGTCGACjCTCGAG|GGGGGGCCCGGTACCCAATTCGCCCTATAGTGAGT CGTATTACAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTG GCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAA TAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGG CGAATGGAAATTGTAAGCGTTAATATTTTGTTAAAATTCGCGTTAAA'ITTTTGTT AAATCAGCTCATTTTTTAACCAATAGGCCGAAATCGGCAAAATCCCTTATAAAT CAAAAGAATAGACCGAGATAGGGTTGAGTGTTGTTCCAGTTTGGAACAAGAGT CCACTATTAAAGAACGTGGACTCCAACGTCAAAGGGCGAAAAACCGTCTATCA GGGCGATGGCCCACTACGTGAACCATCACCCTAATCAAGTTTTTTGGGGTCGAG GTGCCGTAAAGCACTAAATCGGAACCCTAAAGGGAGCCCCCGATTTAGAGCTTG ACGGGGAAAGCCGGCGAACGTGGCGAGAAAGGAAGGGAAGAAAGCGAAAGGA GCGGGCGCTAGGGCGCTGGCAAGTGTAGCGGTCACGCTGCGCGTAACCACCAC ACCCGCCGCGCTTAATGCGCCGCTACAGGGCGCGTCAGGTGGCACTTTTCGGGG AAATGTGCGCGGAACCCCTATTTGTTTATTT7TCTAAATACATTCAAATATGTAT CCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAG AGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTIOCGGCATTTTG CCT CCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGA TCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGAT CCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTTAAAGTT CTGCTATGTGGCGCGGTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGT CGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAA AAGCATCTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACC ATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACGATCGGAGGACCGAA GGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGATCG TTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGA TGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTA CTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAG GACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGG AGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAA GCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGA ACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACT GTCAGACCAAGTTTACTCATATATACTTTAGATTGATTTAAAACTTCATTTTTAA TTTAAAAGGATCTAGGTGAAGATCCTTTTTGATAATCTCATGACCAAAATCCCTT AACGTGAGTTTTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGAT CTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACC ACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCG AAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAG CCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCT CTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCG GGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACG GGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAG ATACCTACAGCGTGAGCTATGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGG CGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGA GCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTC TGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAA AACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTC ACATGTTCTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTT GAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGT GAGCGAGGAAGCGGAAGAGCGCCCAATACGCAAACCGCCTCTCCCCGCGCGTT GGCCGATTCATTAATGCAGCTGGCACGACAGGTTTCCCGACTGGAAAGCGGGCA GTG AG CGCAACGC AATTA ATGTG AGTTAGCTC ACTC ATTAG GCACCCC AG GCTT TACACTTTATGCTTCCGGCTCGTATGTTGTGTGGAATTGTGAGCGGATAACAATT TCACACAGGAAACAGCTATGACCATGATTACGCCAAGCTCGAAATl ^{^} AACCCTCA

CTA A AGG G AACAAA AGCTGGAGCTCC ACCGCGGTGGCG G CjCTCG AGjGTCG AG A TCCGGTCGACCAGCAACCATAGTCCCGCCCCTAACTCCGCCCATCCCGCCCCTA ACTC CGCCCA GTTCCG CCC ATTCTCCGC CCCATGGCTGACTAATTTTTTTTATTTA TGCAGAGGCCGAGGCCGCCTCGGCCTCTGAGCTATTCCAGAAGTAGTGAGGAG GCTTTTTTGGAGGCCTAGGCTTTTGCAAAAAGCTTCGACGGTATCGATTGGCTCA TGTCCAACATTACCGCCATGTTGACATTGATTATTGACTAGTTATTAATAGTAAT CAATTACGGGGTCA ITAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAAC TTACGGTAAATGGCCCGCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGT CAATAATGACGTATGTTCCCATAGTAACGCCAATAGGGACTTTCCATTGACGTC AATGGGTGGAGTATTTACGGTAAACTGCCCACTTGGCAGTACATCAAG'1'GTA TC ATATGCCAAGTACGCCCCCTATTGACGTCAATGACGGTAAATGGCCCGCCTGGC ATTATGCCCAGTACATGACCTTATGGGACTTTCCTACTTGGCAGTACATCTACGT ATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACATCAATGGGCG TGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGTCAA TGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAACAA CTCCGCCCCATTGACGCAAATGGGCGGTAGGCGTGTACGGAATTCGGAGTGGCG AGCCCTCAGATCCTGCATATAAGCAGCTGCTTTTTGCCTGTACTGGGTCTCTCTG

Table 20

Leader _R11 - Hinge- CD28tm/41BB-Z-T2A-tEGFR (SEQ ID NO:48)

Leader

MLLLVTSLLLCELPHPAFLL1P

ScFv Rll

QSVKESEGDLVTPAGNLTLTCTASGSDINDWISWWQAPGKGLEWIGFINSGGSTW YASWV GRFTISRTSTTVDLK TSLTTDDTATYFCARGYSTYYGDFNTWGPGTLVT ISSGGGGSGGGGSGGGGSELVMTQTPSSTSGAVGGTVTINCQASQSIDSNLA FQQ KPGQPPTLLIYRASNLASGVPSRESGSRSGTEYTLTISGVQREDAATYYCLGGVGNV SYRTSFGGGTEVWK

Spacer/Hinge

ESKYGPPCPPCP CD28tm

MFWVLVVVGGVLACYSLLVTVAFIIFWV

4-1BB

KRGR I LLYIF QPFMRPVQTTQEEDGCSCRFPEEEEGGCEL CD3zeta

RVKFSRSADAPAYQQGQNQLYNELNLGRREEYDVLDKRRGRDPEMGGKPRRKNP QEGLY ELQK KMAEAYSEIGMKGERRRGKGHDGLYQGLSTATI DTYDALHMQ ALPPR

T2A

LEGGGEGRGSLLTCGDVEENPGPR

tEGFR

MLLL VTSLLLCELPFIPAFLLIPR VC GIGIGEFKD SLSIN ATNIKHFKNCTSIS GDLHI LPVAFRGDSFTHTPPLDPQELDILKTVKEITGFLLIQAWPENRTDLHAFENLEIIRGRT KQHGQFSLAVVSLNITSLGLRSL EJSDGDVUSGN NLCYANTINWKKJLFGTSGQ T II SNRGEN SCK ATGQ VCH ALCS PEGC WGPEPRDC VSCRN VS RGREC VDKCNLLE GEPREFVENSECIQCHPECLPQAMN1TCTGRGPDNC1QCAH Y 1 DGPI ICVKTCPAGVM GENNTLVWKYADAGHVCHLCFJPNCTYGCTGPGLEGCPT GP IPSIATGMVGALL LLLVVALGIGLFM Table 21

Intermediate Spacer (SEQ ID NO:49)

Hinge/spacer

ESKYGPPCPPCP CH3

GQPREPQVYTLPPSQEEMTKNQVSLT^V GFYPSDIAVEWESNGQPENNYKTTPP VLDSDGSFFLYSRLTVD SRWQEGNVFS|C|SVMHEALHNHYTQKSLSLSLGK

Long spacer (SEQ ID NO: 50)

Hinge

ESKYGPPCPPCP CH2

APEFLGGPSVFLFPPKPKDTLMISRTPEVTjgvVVDVSQEDPEVQFNWYVDGVEVHN

AKTKPREEQFNSTYRWSVLTVLHQDWLNGKEYKgKVSNKGLPSSIEKTISKAK

CH3

GQPREPQVYTLPPSQEEMTKNQVSLT^ GFYPSDIAVEWESNGQPE YKTTPP VLDSDGSFFLYSPJ.T^KSRWQEGNVFSlcjsVMHEALHlslFIYTQKSLSLSLGK

Table 22 Her 2 construct-short spacer (SEQ Id No:54 )

GMCSFss-Her2scFv-IgG4hinge-CD28tm-41BB-Zeta-T2A-EGFRt

Leader

Atgcttctcctggtgacaagccttctgctctgtgagttaccacacccaecattcctcctg atccca

Her2scFV

gatatccagatgacccagtccccgagctccctgtccgcctctgtgggcgatagggtc accatcacctgccgtgccagtcaggatgtg aatactgctgtagcctggtatcaacagaaaccaggaaaagctccgaaactactgatttac tcggcatccttcctctactctggagtccct tctcgcttctctggttccagatctgggacggatttcactctgaccatcagcagtctgcag ccggaagacttcgcaacttattactgtcag caacattatactactcctcccacgttcggacagggtaccaaggtggagatcaaaggcagt actagcggcggtggctccgggggcg gatccggtgggggcggcagcagcgaggttcagctggtggagtctggcggtggcctggtgc agccagggggctcactccgtttgte ctgtgcagcttctggcttcaacattaaagacacctatatacactgggtgcgtcaggcccc ggg aagggcctggaatgggttgcaag gatttatcctacgaatggttatactagatatgccgatagcgtcaagggccgtttcactat aagcgcagacacatccaaaaacacagcct acctgcagatgaacagcctgcgtgctgaggacactgccgtctattattgttctagatggg gaggggacggcttctatgctatggacta ctggggtcaaggaaccctggtcaccgtctcgagt

Hinge spacer

Gagagcaagtacggaccgccctgccccccttgccct

CD28tm

atgttctgggtgctggtggtggtcggaggcgtgctggcctgctacagcctgctggtc accgtggccttcatcatcttttgggtg 4-1BB

Aaacggggcagaaagaaactcctgtatatattcaaacaaccatttatgagaccagtacaa actactcaagaggaagatggctgtagc tgccgatttccagaagaagaagaaggaggatgtgaactg

CD3 zeta

Cgggtgaagttcagcagaagcgccgacgcccctgcctaccagcagggccagaatcagctg tacaacgagctgaacctgggcag aagggaagagtacgacgtcctggataagcggagaggccgggaccctgagatgggcggcaa gcctcggcggaagaacccccag gaaggcctgtataacgaactgcagaaagacaagatggccgaggcctacagcgagatcggc atgaagggcgagcggaggcggg gcaagggccacgacggcctgtatcagggcctgtccaccgccaccaaggatacctacgacg ccctgcacatgcaggccctgcccc caagg

T2A

Ctcgagggcggcggagagggcagaggaagtcttctaacatgcggtgacgtggaggag aatcccggccctagg tEGFR

atgcttctcctggtgacaagccttctgctctgtgagttaccacacccagcattcctc ctgatcccacgcaaagtgtgtaacggaataggt attggtgaatttaaagactcactctccataaatgctacgaatattaaacacttcaaaaac tgcacctccatcagtggcgatctccacatcc tgccggtggcatttaggggtgactccttcacacatactcctcctctggatccacaggaac tggatattctgaaaaccgtaaaggaaatc acagggtttttgctgattcaggcttggcctgaaaacaggacggacctccatgcctttgag aacctagaaatcatacgcggcaggacc aagcaacatggtcagttttctcttgcagtcgtcagcctgaacataacatccttgggatta cgctccctcaaggagataagtgatggaga tgtgataatttcaggaaacaaaaatttgtgctatgcaaatacaataaactggaaaaaact gtttgggacctccggtcagaaaaccaaaa ttataagcaacagaggtgaaaacagctgcaaggccacaggccaggtctgccatgccttgt gctcccccgagggctgctggggccc ggagcccagggactgcgtctcttgccggaatgtcagccgaggcagggaatgcgtggacaa gtgcaaccttctggagggtgagcc aagggagtttgtggagaactctgagtgcatacagtgccacccagagtgcctgcctcaggc catgaacatcacctgcacaggacgg ggaccagacaactgtatccagtgtgcccactacattgacggcccccactgcgtcaagacc tgcccggcaggagtcatgggagaaa acaacaccctggtctggaagtacgcagacgccggccatgtgtgccacctgtgccatccaa actgcacctacggatgcactgggcca ggtcttgaaggctgtccaacgaatgggcctaagatcccgtccatcgccactgggatggtg ggggccctcctcttgctgctggtggtg gccctggggatcggcctcttcatgtga Table 23

Her 2 construct-intermediate spacer (SEQ Id No: 55 )

Leader

Atgcttctcctggtgacaagccttctgctctgtgagttaccacaccca

Her2scFv

Gcattcctcctgatcccagatatccagatgacccagtccccgagctccctgtccgcctct gtgggcgatagggtcaccatcacctgc cgtgccagtcaggatgtgaatactgctgtagcctggtatcaacagaaaccaggaaaagct ccgaaactactgatttactcggcatcct tcctctactctggagtcccttctcgcttctctggttccagatctgggacggatttcactc tgaccatcagcagtctgcagccggaagactt cgcaacttattactgtcagcaacattatactactcctcccacgttcggacagggtaccaa ggtggagatcaaaggcagtactagcggc ggtggctccgggggcggatccggtgggggcggcagcagcgaggttcagctggtggagtct ggcggtggcctggtgcagccagg gggctcactccgtttgtcctgtgcagcttctggcttcaacattaaagacacctatataca ctgggtgcgtcaggccccgggtaagggc ctggaatgggttgcaaggatttatcctacgaaiggttatactagatatgccgatagcgtc aagggccgtttcactataagcgcagacac atccaaaaacacagcctacctgcagatgaacagcctgcgtgctgaggacactgccgtcta ttattgttctagatggggaggggacgg cttctatgctatggactactggggtcaaggaaccctggtcaccgtctcgagt

Hinge spacer

GagagcaagtacggaccgccctgccccccttgccctGgccagcctagagaaccccaggtg tacaccctgcctcccagccagga agagatgaccaagaaccagg gtccctgacctgcctggtcaaaggcttctaccccagcgatatcgccgiggaatgggagag caac ggccagcccgagaacaactacaagaccaccccccctgtgctggacagcgacggcagcttc ttcctgtactcccggctgaccgtgg acaagagccggtggcaggaaggcaacgtcttcagctgcagcgtgatgcacgaggccctgc acaaccactacacccagaagtccc tgagcctgagcctgggcaag

CD28tm

Atgttctgggtgctggtggtggtcggaggcgtgctggcctgctacagcctgctggtcacc gtggccttcatcatcttttgggtg 4-1BB

Aaacggggcagaaagaaactcctgtatatattcaaacaaccatttatgagaccagtacaa actactcaagaggaagatggctgtagc tgccgatttccagaagaagaagaaggaggatgtgaactg

CD3 zeta

Cgggtgaagttcagcagaagcgccgacgcccctgcctaccagcagggccagaatcagctg tacaacgagctgaacctgggcag aagggaagagtacgacgtcctggataagcggagaggccgggaccctgagatgggcggcaa gcctcggcggaagaacccccag gaaggcctgtataacgaactgcagaaagacaagatggccgaggcctacagcgagatcggc atgaagggcgagcggaggcggg gcaagggccacgacggcctgtatcagggcctgtccaccgccaccaaggatacctacgacg ccctgcacatgcaggccctgcccc caagg

T2A

Ctegagggcggcggagagggcagaggaagtcttctaacatgcggtgacgtggaggagaat cccggccctagg

tEGFR

atgcttctcctggtgacaagccttctgctctgtgagttaccacacccagcattcctc ctgatcccacgcaaagtgtgtaacggaataggt attggtgaatttaaagactcactctccataaatgctacgaatattaaacacitcaaaaac tgcacctccatcagtggcgatctccacatcc tgccggtggcatttaggggtgactccttcacacatactcctcctctggatccacaggaac tggatattctgaaaaccgtaaaggaaatc acagggtttttgctgattcaggcttggcctgaaaacaggacggacctccatgcctttgag aacctagaaatcatacgcggcaggacc aagcaacatggtcagttttctcttgcagtcgtcagcctgaacataacatccttgggatta cgctccctcaaggagataagtgatggaga tgtgataatttcaggaaacaaaaatttgtgctatgcaaatacaataaactggaaaaaact gtttgggacctccggtcagaaaaccaaaa ttataagcaacagaggtgaaaacagctgcaaggccacaggccaggtctgccatgccttgt gctcccccgagggctgctggggccc ggagcccagggactgcgtctcttgccggaatgtcagccgaggcagggaatgcgtggacaa gtgcaaccttctggagggtgagcc aagggagtttgtggagaactctgagtgcatacagtgccacccagagtgcctgcctcaggc catgaacatcacctgcacaggacgg ggaccagacaactgtatccagtgtgcccactacattgacggcccccactgcgtcaagacc tgcccggcaggagtcatgggagaaa acaacaccctggtctggaagtacgcagacgccggccatgtgtgccacctgtgccatccaa actgcacciacggatgcactgggcca ggtcttgaaggctgtccaacgaatgggcctaagatcccgtccatcgccactgggatggtg ggggccctcctcttgcigctggtggtg gccctggggatcggcctcttcatgtga Table 24

Her 2 construct-long spacer (SEQ Id No: 56 )

leader

Atgcttctcctggtgacaagccttctgctctgtgagttaccacaccca

Her2scFV

gcattcctcctgatcccagatatccagatgacccagtccccgagctccctgtccgcc tctgtgggcgatagggtcaccatcacctgcc gtgccagtcaggatgtgaatactgctgtagcctggtatcaacagaaaccaggaaaagctc cgaaactactgatttactcggcatcctt cctctactctggagtcccttctcgcttctctggttccagatctgggacggatttcactct gaccatcagcagtctgcagccggaagactt cgcaacttattactgtcagcaacattatactactcctcccacgttcggacagggtaccaa ggtggagatcaaaggcagtactagcggc ggtggctccgggggcggatccggtgggggcggcagcagcgaggttcagctggtggagtct ggcggtggcctggtgcagccagg gggctcactccgtttgtcctgtgcagcttctggcttcaacattaaagacacctatataca ctgggtgcgtcaggccccgggtaagggc ctggaatgggttgcaaggatttatcctacgaatggttatactagatatgccgatagcgtc aagggccgtttcactataagcgcagacac atccaaaaacacagcctacctgcagatgaacagcctgcgtgctgaggacactgccgtcta ttattgttctagatggggaggggacgg cttctatgctatggactactggggtcaaggaaccctggtcaccgtctcgagt

long spacer

gagagcaagtacggaccgccctgccccccttgccctgcccccgagttcctgggcgga cccagcgtgttcctgttcccccccaagcc caaggacaccctgatgatcagccggacccccgaggtgacctgcgtggtggtggacgtgag ccaggaagatcccgaggtccagtt caattggtacgtggacggcgtggaagtgcacaacgccaagaccaagcccagagaggaaca gttcaacagcacctaccgggtggt gtctgtgctgaccgtgctgcaccaggactggctgaacggcaaagaatacaagtgcaaggt gtccaacaagggcctgcccagcagc atcgaaaagaccatcagcaaggccaagggccagcctcgcgagccccaggtgtacaccctg cctccctcccaggaagagatgacc aagaaccaggtgtccctgacctgcctggt aagggcttctaccccagcgacatcgccgtggagtgggagagcaacggccagcct gagaacaactacaagaccacccctcccgtgctggacagcgacggcagcttcttcctgtac agccggctgaccgtggacaagagcc ggtggcaggaaggcaacgtctttagctgcagcgtgatgcacgaggccctgcacaaccact acacccagaagagcctgagcctgtc cctgggcaag

CD28tm

atgttctgggtgctggtggtggtgggcggggtgctggcctgctacagcctgctggtg acagtggccttcatcatcttttgggtg 4- IBB

aaacggggcagaaagaaactcctgtalatattcaaacaaccatttatgagaccagta caaactactcaagaggaagatggctgtagct gccgatttccagaagaagaagaaggaggatgtgaactg

CD3zeta

Cgggtgaagttcagcagaagcgccgacgcccctgcctaccagcagggccagaatcagctg tacaacgagctgaacctgggcag aagggaagagtacgacgtcctggataagcggagaggccgggaccctgagaigggcggcaa gcctcggcggaagaacccccag gaaggcctgtataacgaactgcagaaagacaagatggccgaggcctacagcgagatcggc atgaagggcgagcggaggcggg gcaagggccacgacggcctgtatcagggcctgtccaccgccaccaaggatacctacgacg ccctgcacatgcaggccctgcccc caagg

T2A

Ctcgagggcggcggagagggcagaggaagtcttctaacatgcggtgacgtggaggagaat cccggccctagg

tEGFR

atgcttctcctggtgacaagccttctgctctgtgagttaccacacccagcattectc ctgatcccacgcaaagtgtgtaacggaataggt attggtgaatttaaagactcactctccataaatgctacgaatattaaacacttcaaaaac tgcacctccatcagtggcgatctccacatcc tgccggtggcatttaggggtgactccttcacacatactcctcctctggatccacaggaac tggatattctgaaaaccgtaaaggaaatc acagggtttttgctgattcaggcttggcctgaaaacaggacggacctccatgcctttgag aacctagaaatcatacgcggcaggacc aagcaacatggtcagttttctcttgcagtcgtcagcctgaacataacatGcttgggatta cgctccctcaaggagataagtgatggaga tgtgataatttcaggaaacaaaaatttgtgctatgcaaatacaataaactggaaaaaact gtttgggacctccggtcagaaaaccaaaa ttataagcaacagaggtgaaaacagctgcaaggccacaggccaggtctgccatgccttgt gctcccccgagggctgctggggccc ggagcccagggactgcgtctcttgccggaatgtcagccgaggcagggaatgcgtggacaa gtgcaaccttctggagggtgagcc aagggagtttgtggagaactctgagtgcatacagtgccacccagagtgcctgcctcaggc catgaacatcacctgcacaggacgg ggaccagacaactgtatccagtgtgcccactacattgacggcccccactgcgtcaagacc tgcccggcaggagtcatgggagaaa acaacaccctggtctggaagtacgcagacgccggccatgtgtgccacctgtgccatccaa actgcacctacggatgcactgggcca ggtcttgaaggctgtccaacgaatgggcctaagatcccgtccatcgccactgggatggtg ggggccctcctcttgctgctggtggtg gccctggggatcggcctcttcatgtga