Title:
METHOD FOR COMPREHENSIVE DETECTION OF FIVE TYPES OF VIRUSES HIV, HCV, HBV, PVB19 AND WNV EACH HAVING MULTIPLE GENOTYPES, AND PRIMER SETS, MICROARRAYS AND KIT FOR DETECTION OF VIRUSES
Document Type and Number:
WIPO Patent Application WO/2011/074181
Kind Code:
A1
Abstract:
Disclosed are: a method for comprehensively detecting five types of viruses HIV, HCV, HBV, PvB19 and WNV each having multiple genotypes with high specificity and high sensitivity; and primer sets, microarrays and a kit for use in the detection of viruses.
Each of HIV, HCV, HBV, PvB19 and WNV each having a multiple genotypes can be detected by amplifying a gene of each of HIV, HCV, HBV, PvB19 and WNV by PCR using a genotype-specific primer and hybridizing a PCR amplification product with a DNA microarray. By using primer sets and probe sets for virus detection uses, five types of viruses HIV, HCV, HBV, PvB19 and WNV each having multiple genotypes can be detected with high specificity and high sensitivity.
Inventors:
NAKAJIMA TATSUO (JP)
HAMAGUCHI ISAO (JP)
TAKIZAWA KAZUYA (JP)
MIZUTANI TETSUYA (JP)
ENDO DAIJI (JP)
HAMAGUCHI ISAO (JP)
TAKIZAWA KAZUYA (JP)
MIZUTANI TETSUYA (JP)
ENDO DAIJI (JP)
Application Number:
PCT/JP2010/006687
Publication Date:
June 23, 2011
Filing Date:
November 15, 2010
Export Citation:
Assignee:
NIHON PARKERIZING HIROSHIMA WORKS CO LTD (JP)
NAT INST OF INFECTIOUS DISEASES (JP)
RAKUNO GAKUEN UNIVERSITY (JP)
NAKAJIMA TATSUO (JP)
HAMAGUCHI ISAO (JP)
TAKIZAWA KAZUYA (JP)
MIZUTANI TETSUYA (JP)
ENDO DAIJI (JP)
NAT INST OF INFECTIOUS DISEASES (JP)
RAKUNO GAKUEN UNIVERSITY (JP)
NAKAJIMA TATSUO (JP)
HAMAGUCHI ISAO (JP)
TAKIZAWA KAZUYA (JP)
MIZUTANI TETSUYA (JP)
ENDO DAIJI (JP)
International Classes:
C12N15/09; C12M1/00; C12Q1/68
Domestic Patent References:
| WO2003087361A1 | 2003-10-23 |
Other References:
KAZUSHIGE YAMAGUCHI ET AL.: "Kyotsu Motif Shugo primer Yosoku Algorithm (CoCoMo) o Mochiita HIV, HCV Oyobi HBV-yo degenerate primer no Sekkei", KOSEI RODO KAGAKU KENKYUHI HOJOKIN, 24 April 2009 (2009-04-24), pages 5 - 49
DAIJI ENDO: "Virus Kabu Kan Kyotsu Primer o Mochiita Shinraisei no Takai Virus Kenshutsu Hoho no Kento", MINISTRY OF HEALTH, LABOUR AND WELFARE KAGAKU KENKYUHI HOJOKIN, 24 April 2009 (2009-04-24), pages 21 - 71
DAIJI ENDO ET AL.: "Kyotsu Oligomer Bunseki ni Motozuku Tashu Virus Kyotsu Primer no Sekkei", JOURNAL OF VETERINARY MEDICINE, vol. 62, no. 19, 1 October 2009 (2009-10-01), pages 823 - 824
DAIJI ENDO: "Virus Kabu Kan Kyotsu Primer o Mochiita Shinraisei no Takai Virus Kenshutsu Hoho no Kento", MINISTRY OF HEALTH, LABOUR AND WELFARE KAGAKU KENKYUHI HOJOKIN, 24 April 2009 (2009-04-24), pages 21 - 71
DAIJI ENDO ET AL.: "Kyotsu Oligomer Bunseki ni Motozuku Tashu Virus Kyotsu Primer no Sekkei", JOURNAL OF VETERINARY MEDICINE, vol. 62, no. 19, 1 October 2009 (2009-10-01), pages 823 - 824
Attorney, Agent or Firm:
OHTA PATENT OFFICE (JP)
Patent business corporation Ota patent firm (JP)
Patent business corporation Ota patent firm (JP)
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