Title:
METHOD OF CONCENTRATING GENE
Document Type and Number:
WIPO Patent Application WO/2002/050268
Kind Code:
A1
Abstract:
A method whereby a gene expressed only in a trace amount can be concentrated from a mixture of a gene expressed in a large amount with the gene expressed only in a trace amount, even in case where the gene expressed in a large amount is unknown.
Inventors:
NAKAZATO HIROSHI (JP)
Application Number:
PCT/JP2001/011113
Publication Date:
June 27, 2002
Filing Date:
December 18, 2001
Export Citation:
Assignee:
SUNTORY LTD (JP)
SUNTORY BIOMEDICAL RES LTD (JP)
NAKAZATO HIROSHI (JP)
SUNTORY BIOMEDICAL RES LTD (JP)
NAKAZATO HIROSHI (JP)
International Classes:
C12N15/10; (IPC1-7): C12N15/10; C12Q1/68; C12N1/00
Foreign References:
| EP0959141A2 | 1999-11-24 | |||
| JP2000325080A | 2000-11-28 | |||
| EP0959141A2 | 1999-11-24 |
Other References:
WIELAND I. ET AL.: "A method for difference cloning: gene amplification following subtractive hybridization", PROC. NATL. ACAD. SCI. USA, vol. 87, no. 7, April 1990 (1990-04-01), pages 2720 - 2724, XP002909431
KO M.S. ET AL.: "An equalized cDNA library by the reassociation of short double-stranded cDNAs", NUCLEIC ACIDS RES., vol. 18, no. 19, 11 October 1990 (1990-10-11), pages 5705 - 5711, XP002086978
DIATCHENKO L.: "Suppression subtractive hybridization: a method for generating differentially regulated or tissue-specific cDNA probes and libraries", PROC. NATL. ACAD. SCI. USA, vol. 93, no. 12, 11 June 1996 (1996-06-11), pages 6025 - 6030, XP002911922
SCIENCE, vol. 258, 1992, pages 818 - 821
NIKOLAI LISITSYN ET AL., SCIENCE, vol. 259, 1993, pages 946 - 951
CHO; PARK, NUCLEIC ACIDS RESEARCH, vol. 26, no. 6, 1998, pages 1440 - 1448
MILNER ET AL., NUCLEIC ACIDS RESEARCH, vol. 23, no. 1, 1995, pages 176 - 187
WIELAND ET AL., PROC.NATL.ACAD.SCI. USA, vol. 87, 1990, pages 2720 - 2724
KO, NUCLEIC ACIDS RESEARCH, vol. 18, no. 19, 1990, pages 5705 - 5711
DIATCHENKO ET AL., METHODS IN ENZYMOLOGY, vol. 303, 1999, pages 349 - 380
KO M.S. ET AL.: "An equalized cDNA library by the reassociation of short double-stranded cDNAs", NUCLEIC ACIDS RES., vol. 18, no. 19, 11 October 1990 (1990-10-11), pages 5705 - 5711, XP002086978
DIATCHENKO L.: "Suppression subtractive hybridization: a method for generating differentially regulated or tissue-specific cDNA probes and libraries", PROC. NATL. ACAD. SCI. USA, vol. 93, no. 12, 11 June 1996 (1996-06-11), pages 6025 - 6030, XP002911922
SCIENCE, vol. 258, 1992, pages 818 - 821
NIKOLAI LISITSYN ET AL., SCIENCE, vol. 259, 1993, pages 946 - 951
CHO; PARK, NUCLEIC ACIDS RESEARCH, vol. 26, no. 6, 1998, pages 1440 - 1448
MILNER ET AL., NUCLEIC ACIDS RESEARCH, vol. 23, no. 1, 1995, pages 176 - 187
WIELAND ET AL., PROC.NATL.ACAD.SCI. USA, vol. 87, 1990, pages 2720 - 2724
KO, NUCLEIC ACIDS RESEARCH, vol. 18, no. 19, 1990, pages 5705 - 5711
DIATCHENKO ET AL., METHODS IN ENZYMOLOGY, vol. 303, 1999, pages 349 - 380
Attorney, Agent or Firm:
Iwatani, Ryo (5F 1-27 Dojima 2-chom, Kita-ku Osaka-shi Osaka, JP)
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