Title:
METHOD FOR CONSTRUCTING ENGINEERED CORYNEBACTERIUM GLUTAMICUM BACTERIA HAVING HIGH PRODUCTION OF L-LYSINE
Document Type and Number:
WIPO Patent Application WO/2017/186026
Kind Code:
A1
Abstract:
Provided is a method for constructing engineered corynebacterium glutamicum bacteria having a high production of L-lysine. The engineered bacteria are constructed by deactivating the NCgl1221 gene of corynebacterium glutamicum, and the nucleotide sequence of the NCgl1221 gene encoding frame is shown as set forth in SEQ ID NO. 1.
Inventors:
WANG RUIMING (CN)
WANG JUNQING (CN)
WANG LEI (CN)
WANG TENGFEI (CN)
LI GUIHUA (CN)
WANG JIANBIN (CN)
SUI SONGSEN (CN)
WANG JUNQING (CN)
WANG LEI (CN)
WANG TENGFEI (CN)
LI GUIHUA (CN)
WANG JIANBIN (CN)
SUI SONGSEN (CN)
Application Number:
PCT/CN2017/080902
Publication Date:
November 02, 2017
Filing Date:
April 18, 2017
Export Citation:
Assignee:
UNIV QILU TECHNOLOGY (CN)
ZHUCHENG DONGXIAO BIOTECHNOLOGY CO LTD (CN)
SHANDONG PROVINCIAL FEED QUALITY INSPECTION INST (CN)
ZHUCHENG DONGXIAO BIOTECHNOLOGY CO LTD (CN)
SHANDONG PROVINCIAL FEED QUALITY INSPECTION INST (CN)
International Classes:
C12N1/21; C12N15/90; C12P13/08; C12R1/15
Foreign References:
| CN105950524A | 2016-09-21 | |||
| CN101600796A | 2009-12-09 | |||
| CN101605886A | 2009-12-16 | |||
| CN103282486A | 2013-09-04 |
Other References:
JIANG, YANZHE ET AL.: "Effects of NCgl 1221 Knockout and pyc Overpression on Glutamine Fermentation", MODERN FOOD SCIENCE & TECHNOLOGY, vol. 30, no. 10, 31 December 2014 (2014-12-31), pages 96 - 101, XP009508135, ISSN: 1673-9078
JUN NAKAMURA ET AL.: "Mutations of the Corynebacterium glutamicum NCg11221 Gene , Encoding a Mechanosensitive Channel Homolog, Induce 1-Glutamic Acid Production", APPLIED AND ENVIROMENTAL MICROBIOLOGY, vol. 14, no. 73, 31 July 2007 (2007-07-31), pages 4491 - 4498, XP002691368, ISSN: 0099-2240
CHIKAKO YAMASHITAE ET AL.: "L-Glutamate secretion by the N-terminal domain of the Corynebacterim glutamicum NCg11221 mechanosensitive channel", BIOSCIENCE. BIOTEHNOLOGY . BIOCHEMISTRY, vol. 77, no. 5, 7 May 2013 (2013-05-07), pages 1008 - 1013, XP055436377, ISSN: 1347-6947
JUN NAKAMURA ET AL.: "Mutations of the Corynebacterium glutamicum NCg11221 Gene , Encoding a Mechanosensitive Channel Homolog, Induce 1-Glutamic Acid Production", APPLIED AND ENVIROMENTAL MICROBIOLOGY, vol. 14, no. 73, 31 July 2007 (2007-07-31), pages 4491 - 4498, XP002691368, ISSN: 0099-2240
CHIKAKO YAMASHITAE ET AL.: "L-Glutamate secretion by the N-terminal domain of the Corynebacterim glutamicum NCg11221 mechanosensitive channel", BIOSCIENCE. BIOTEHNOLOGY . BIOCHEMISTRY, vol. 77, no. 5, 7 May 2013 (2013-05-07), pages 1008 - 1013, XP055436377, ISSN: 1347-6947
Attorney, Agent or Firm:
JINAN JINDI INTELLECTUAL PROPERTY AGENCY CO., LTD. (CN)
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