METHOD OF CONSTRUCTING GRAPE ANTIBODY LIBRARY

WIPO Patent Application WO/2021/012704

A1

The present invention provides a method of constructing a grape antibody library, comprising the steps of: extracting a grape protein antigen, immunizing mice with the obtained protein antigen; fusing the spleen and lymphocyte cells of the immunized mouse with SP2/0 mouse myeloma cells to obtain hybridoma cells, and preparing an antibody; spotting the obtained antibody on chips to obtain grape antibody chips; screening the obtained grape antibody chips, and obtaining a enriched specific antibody library targeting proteins fixed on the grape protein chips through data analysis.

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ZHANG YING (CN)
LIU CHONGHUAI (CN)
LIU RUITAO (CN)
FAN XIUCAI (CN)
LI MIN (CN)
JIANG JIANFU (CN)

PCT/CN2020/081956

January 28, 2021

March 29, 2020

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ZHENGZHOU FRUIT RES INSTITUTE CAAS (CN)

C40B50/06; C07K16/16; C40B40/10

CN110484974A	2019-11-22
CN1880447A	2006-12-20
CN103808922A	2014-05-21
CN104379821A	2015-02-25
CN105063758A	2015-11-18
CN104655851A	2015-05-27
CN1374525A	2002-10-16

MOHAMMAD S. ET AL.: "Generation and Characterization of Anti-Chitinase Monoclonal Antibodies", HYBRIDOMA (LARCHMT), vol. 30, no. 2,, 1 May 2011 (2011-05-01), XP055775141

SCIHEAD IP LAW FIRM (CN)

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