Title:
METHOD FOR CONSTRUCTING LIBRARY OF CELL-FREE DNAS IN BODY FLUIDS AND APPLICATION THEREOF
Document Type and Number:
WIPO Patent Application WO/2019/024341
Kind Code:
A1
Abstract:
A method for constructing a library of cell-free DNAs in body fluids, comprising directly acting a transposase or an endonuclease on a body fluid sample, fragmenting the cell-free DNAs within, and performing amplification to obtain a library. Also provided is a test kit using the present method for prenatal diagnosis or early detection of cancer.
Inventors:
WANG MINGYUE (CN)
LIU LONGQI (CN)
LIU CHUANYU (CN)
WU LIANG (CN)
XU JIANGSHAN (CN)
YUAN YUE (CN)
SHANG ZHOUCHUN (CN)
LIU LONGQI (CN)
LIU CHUANYU (CN)
WU LIANG (CN)
XU JIANGSHAN (CN)
YUAN YUE (CN)
SHANG ZHOUCHUN (CN)
Application Number:
PCT/CN2017/113208
Publication Date:
February 07, 2019
Filing Date:
November 27, 2017
Export Citation:
Assignee:
BGI SHENZHEN (CN)
International Classes:
C40B50/06; C12N15/10; C12Q1/68
Domestic Patent References:
| WO2007018601A1 | 2007-02-15 |
Foreign References:
| CN104357918A | 2015-02-18 | |||
| CN104789553A | 2015-07-22 | |||
| CN104450923A | 2015-03-25 |
Other References:
LI, YANFANG ET AL.: "Clinical Experience of Noninvasive Prenatal Testing with Fetal Cell -Free DNA for Trisomy 21, 18 and 13", CHINESE JOURNAL OF CLINICAL OBSTETRICS AND GYNECOLOGY, vol. 16, no. 2, 31 March 2015 (2015-03-31), pages 126 - 129, XP009520821, ISSN: 1672-1861, DOI: 10.13390/j.issn.1672-1861.2015.02.009
LO ET AL., AM. J. HUM. GENET, vol. 64, 1999, pages 218 - 224
MORELLI ET AL., ANN ONCOL, vol. 26, 2015, pages 731 - 736
LO ET AL., PNAS, vol. 112, 2015, pages 5503 - 5512
SHENDURE ET AL., CELL, vol. 164, 2016, pages 57 - 68
SPEICHER ET AL., NATURE GENETICS, vol. 48, 2016, pages 1273 - 1278
CHANG ET AL., CURR. PROTOC. MOL. BIOL., vol. 109
See also references of EP 3719182A4
LO ET AL., AM. J. HUM. GENET, vol. 64, 1999, pages 218 - 224
MORELLI ET AL., ANN ONCOL, vol. 26, 2015, pages 731 - 736
LO ET AL., PNAS, vol. 112, 2015, pages 5503 - 5512
SHENDURE ET AL., CELL, vol. 164, 2016, pages 57 - 68
SPEICHER ET AL., NATURE GENETICS, vol. 48, 2016, pages 1273 - 1278
CHANG ET AL., CURR. PROTOC. MOL. BIOL., vol. 109
See also references of EP 3719182A4
Attorney, Agent or Firm:
BEYOND ATTORNEYS AT LAW (CN)
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