Title:
METHOD FOR CONTINUOUSLY ENRICHING AN OIL PRODUCED BY MICROALGAE WITH ETHYL ESTERS OF DHA
Document Type and Number:
WIPO Patent Application WO/2013/178937
Kind Code:
A3
Abstract:
The invention concerns a method of preparing an oil enriched with ethyl esters of DHA produced by fermenting microorganisms, characterised in that it comprises a step of purification by extraction by supercritical CO2 on a multistage fractionating column operating with a counter-current with reflux extraction.
Inventors:
PERRUT MICHEL (FR)
PERRUT VINCENT (FR)
LOOTEN PHILIPPE (FR)
PERRUT VINCENT (FR)
LOOTEN PHILIPPE (FR)
Application Number:
PCT/FR2013/051187
Publication Date:
April 24, 2014
Filing Date:
May 28, 2013
Export Citation:
Assignee:
ROQUETTE FRERES (FR)
International Classes:
A23D9/02; A23L1/30; C11B1/10; C11B3/00; C11B3/14; C11B7/00; C11C1/10; C11C3/00
Domestic Patent References:
| WO1994028913A1 | 1994-12-22 |
Foreign References:
| US7365219B2 | 2008-04-29 | |||
| FR2894772A1 | 2007-06-22 | |||
| US20110263709A1 | 2011-10-27 |
Other References:
YAMAMURA ET AL: "INDUSTRIAL HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY PURIFICATION OF DOCOSAHEXAENOIC ACID ETHYL ESTER AND DOCOSAPENTAENOIC ACID ETHYL ESTER FRO MSINGLE-CELL OIL", JOURNAL OF THE AMERICAN OIL CHEMISTS' SOCIETY, SPRINGER, DE, vol. 74, no. 11, 1 January 1997 (1997-01-01), pages 1435 - 1440, XP002166746, ISSN: 0003-021X, DOI: 10.1007/S11746-997-0250-6
DATABASE WPI Week 200977, Derwent World Patents Index; AN 2009-Q29681, XP002693414
FLECK U ET AL: "Fractionation of fatty acid ethyl esters by supercritical CO2: high separation efficiency using an automated countercurrent column", JOURNAL OF SUPERCRITICAL FLUIDS, PRA PRESS, US, vol. 14, no. 1, 1 October 1998 (1998-10-01), pages 67 - 74, XP004262488, ISSN: 0896-8446, DOI: 10.1016/S0896-8446(98)00100-4
RIHA V ET AL: "Separation of fish oil ethyl esters with supercritical carbon dioxide", JOURNAL OF SUPERCRITICAL FLUIDS, PRA PRESS, US, vol. 17, no. 1, 29 February 2000 (2000-02-29), pages 55 - 64, XP004262567, ISSN: 0896-8446, DOI: 10.1016/S0896-8446(99)00038-8
PERRETTI ET AL: "Supercritical carbon dioxide fractionation of fish oil fatty acid ethyl esters", JOURNAL OF SUPERCRITICAL FLUIDS, PRA PRESS, US, vol. 40, no. 3, 7 March 2007 (2007-03-07), pages 349 - 353, XP005912564, ISSN: 0896-8446, DOI: 10.1016/J.SUPFLU.2006.07.020
DANIEL TENLLADO ET AL: "A combined procedure of supercritical fluid extraction and molecular distillation for the purification of alkylglycerols from shark liver oil", SEPARATION AND PURIFICATION TECHNOLOGY, ELSEVIER SCIENCE, AMSTERDAM, NL, vol. 83, 2 September 2011 (2011-09-02), pages 74 - 81, XP028329582, ISSN: 1383-5866, [retrieved on 20110921], DOI: 10.1016/J.SEPPUR.2011.09.013
BRUNNER G: "Supercritical fluids: technology and application to food processing", JOURNAL OF FOOD ENGINEERING, BARKING, ESSEX, GB, vol. 67, no. 1-2, 1 March 2005 (2005-03-01), pages 21 - 33, XP004619293, ISSN: 0260-8774, DOI: 10.1016/J.JFOODENG.2004.05.060
DATABASE WPI Week 200977, Derwent World Patents Index; AN 2009-Q29681, XP002693414
FLECK U ET AL: "Fractionation of fatty acid ethyl esters by supercritical CO2: high separation efficiency using an automated countercurrent column", JOURNAL OF SUPERCRITICAL FLUIDS, PRA PRESS, US, vol. 14, no. 1, 1 October 1998 (1998-10-01), pages 67 - 74, XP004262488, ISSN: 0896-8446, DOI: 10.1016/S0896-8446(98)00100-4
RIHA V ET AL: "Separation of fish oil ethyl esters with supercritical carbon dioxide", JOURNAL OF SUPERCRITICAL FLUIDS, PRA PRESS, US, vol. 17, no. 1, 29 February 2000 (2000-02-29), pages 55 - 64, XP004262567, ISSN: 0896-8446, DOI: 10.1016/S0896-8446(99)00038-8
PERRETTI ET AL: "Supercritical carbon dioxide fractionation of fish oil fatty acid ethyl esters", JOURNAL OF SUPERCRITICAL FLUIDS, PRA PRESS, US, vol. 40, no. 3, 7 March 2007 (2007-03-07), pages 349 - 353, XP005912564, ISSN: 0896-8446, DOI: 10.1016/J.SUPFLU.2006.07.020
DANIEL TENLLADO ET AL: "A combined procedure of supercritical fluid extraction and molecular distillation for the purification of alkylglycerols from shark liver oil", SEPARATION AND PURIFICATION TECHNOLOGY, ELSEVIER SCIENCE, AMSTERDAM, NL, vol. 83, 2 September 2011 (2011-09-02), pages 74 - 81, XP028329582, ISSN: 1383-5866, [retrieved on 20110921], DOI: 10.1016/J.SEPPUR.2011.09.013
BRUNNER G: "Supercritical fluids: technology and application to food processing", JOURNAL OF FOOD ENGINEERING, BARKING, ESSEX, GB, vol. 67, no. 1-2, 1 March 2005 (2005-03-01), pages 21 - 33, XP004619293, ISSN: 0260-8774, DOI: 10.1016/J.JFOODENG.2004.05.060
Attorney, Agent or Firm:
GALLOIS, Valérie et al. (25 rue Louis Le Grand, F-75 002 Paris, FR)
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