Title:
METHOD FOR CONTINUOUSLY PRODUCING GLUTATHIONE USING PHOTOSYNTHETIC CELL MEMBRANE VESICLE
Document Type and Number:
WIPO Patent Application WO/2016/114618
Kind Code:
A1
Abstract:
The present invention relates to a method for producing glutathione by combining a photosynthetic cell membrane vesicle and an enzyme that catalyzes glutathione synthesis, and by using glutamic acid, cysteine and glycine as reaction substrates. A method of using the enzyme that catalyzes glutathione synthesis includes a method of using gamma glutamylcysteine and glutathione synthetase together, or a method of using bifunctional glutathione synthetase. There has been a problem that expensive adenosine triphosphate must be continuously supplied in the synthesis of glutathione, but the present invention can continuously produce glutathione without additional input of adenosine triphosphate by using the photosynthetic cell membrane vesicle as a supply source of adenosine triphosphate, and thereby may significantly contribute to a reduction in the production costs of glutathione.
Inventors:
LEE JEONG KUG (KR)
KIM HYEON JUN (KR)
OH EUN KYOUNG (KR)
KIM HYEON JUN (KR)
OH EUN KYOUNG (KR)
Application Number:
PCT/KR2016/000433
Publication Date:
July 21, 2016
Filing Date:
January 15, 2016
Export Citation:
Assignee:
UNIV SOGANG RES FOUNDATION (KR)
International Classes:
C12P21/02; C12N9/00
Foreign References:
| CN102220400A | 2011-10-19 | |||
| US20080194701A1 | 2008-08-14 | |||
| CN103627691A | 2014-03-12 |
Other References:
SAWA, YOSHIHIRO ET AL.: "Photosynthetic Glutathione Production using Intact Cyanobacterial Cells", AGRICULTURAL AND BIOLOGICAL CHEMISTRY, vol. 50, no. 5, 1986, pages 1361 - 1363, XP055467362
CREISSEN, GARY ET AL.: "Elevated Glutathione Biosynthetic Capacity in the Chloroplasts of Transgenic Tobacco Plants Paradoxically Causes increased Oxidative Stress", THE PLANT CELL, vol. 11, no. 7, 1999, pages 1277 - 1291, XP002199575
See also references of EP 3246411A4
CREISSEN, GARY ET AL.: "Elevated Glutathione Biosynthetic Capacity in the Chloroplasts of Transgenic Tobacco Plants Paradoxically Causes increased Oxidative Stress", THE PLANT CELL, vol. 11, no. 7, 1999, pages 1277 - 1291, XP002199575
See also references of EP 3246411A4
Attorney, Agent or Firm:
CHOI, Kyu Whan (KR)
최규환 (KR)
최규환 (KR)
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