Title:
METHOD FOR CREATING DATABASE FOR IDENTIFICATION AND QUANTIFICATION OF PEPTIDE PEAKS IN MASS SPECTROMETRY
Document Type and Number:
WIPO Patent Application WO/2015/008453
Kind Code:
A1
Abstract:
The present invention addresses the problem of providing: a method for creating a database for comprehensively identifying/quantifying, with high sensitivity, the peaks of peptides constituting a protein to be examined, through analysis using LC-MS/MS; and a method in which use of the database makes it possible to comprehensively identify/quantify, with high sensitivity, the peaks of peptides constituting a protein to be examined, through analysis using LC-MS/MS. The elution time for each common peptide peak among identified peptide peaks of analyzed samples is used as a reference to normalize the elution time for all the peptide peaks of the analyzed samples. Thus, a database comprising an accumulation of the normalized elution times is created. Using this database, a protein to be examined in a sample is analyzed using LC-MS/MS to identify the peaks of peptides to be examined, and the identified peptide peaks are checked for validation, thereby identifying/quantifying the peaks of peptides constituting the protein to be examined in a sample.
Inventors:
HTSUKI SUMIO (JP)
Application Number:
PCT/JP2014/003600
Publication Date:
January 22, 2015
Filing Date:
July 07, 2014
Export Citation:
Assignee:
UNIV KUMAMOTO NAT UNIV CORP (JP)
International Classes:
G01N27/62
Domestic Patent References:
| WO2011007884A1 | 2011-01-20 | |||
| WO2013014853A1 | 2013-01-31 | |||
| WO2013044401A1 | 2013-04-04 |
Foreign References:
| JP2005091344A | 2005-04-07 |
Other References:
HIROKAZU NAKATSUMI: "New horizons in biological research developed by next-generation proteomics : Say good-bye to Western blotting", JOURNAL OF JAPANESE BIOCHEMICAL SOCIETY, vol. 84, no. 1, January 2012 (2012-01-01), pages 53 - 57
GILLET LC: "Targeted Data Extraction of the MS/MS Spectra Generated by Data-independent Acquisition: A New Concept for Consistent and Accurate Proteome Analysis", MOL CELL PROTEOMICS, vol. 11.6, 18 January 2012 (2012-01-18)
LIU, Y. ET AL.: "Quantitative measurements of N- linked glycoproteins in human plasma by SWATH- MS", PROTEOMICS, vol. 13.8, 11 March 2013 (2013-03-11), pages 1247 - 1256
GILLET LC: "Targeted Data Extraction of the MS/MS Spectra Generated by Data-independent Acquisition: A New Concept for Consistent and Accurate Proteome Analysis", MOL CELL PROTEOMICS, vol. 11.6, 18 January 2012 (2012-01-18)
LIU, Y. ET AL.: "Quantitative measurements of N- linked glycoproteins in human plasma by SWATH- MS", PROTEOMICS, vol. 13.8, 11 March 2013 (2013-03-11), pages 1247 - 1256
Attorney, Agent or Firm:
HIROTA, Masanori (JP)
Masaki Hirota (JP)
Masaki Hirota (JP)
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