Title:
METHOD FOR CULTURE OF CELLS
Document Type and Number:
WIPO Patent Application WO/2019/107485
Kind Code:
A1
Abstract:
Provided as a technique for propagating neural crest cells without lowering the differentiation capacity is a method for producing neural crest cells that includes (1) a step for obtaining neural crest cells and (2) a step for suspension culturing the neural crest cells in medium containing a GSK3β inhibitor and basic fibroblast growth factor (bFGF), the medium including a GSK3β inhibitor of a concentration that presents an effect equivalent to the effect presented by CHIR99021 of a concentration from more than 1 μM to less than 5 μM.
Inventors:
IKEYA MAKOTO (JP)
TOYOOKA YAYOI (JP)
TOYOOKA YAYOI (JP)
Application Number:
PCT/JP2018/043949
Publication Date:
June 06, 2019
Filing Date:
November 29, 2018
Export Citation:
Assignee:
UNIV KYOTO (JP)
TAKEDA PHARMACEUTICALS CO (JP)
TAKEDA PHARMACEUTICALS CO (JP)
International Classes:
C12N5/079; C12N5/071; C12N5/0735; C12N5/10
Domestic Patent References:
| WO2016194522A1 | 2016-12-08 | |||
| WO2016104574A1 | 2016-06-30 | |||
| WO2007069666A1 | 2007-06-21 | |||
| WO2008118220A2 | 2008-10-02 | |||
| WO2008124133A1 | 2008-10-16 | |||
| WO2008151058A2 | 2008-12-11 | |||
| WO2009006930A1 | 2009-01-15 | |||
| WO2009006997A1 | 2009-01-15 | |||
| WO2009007852A2 | 2009-01-15 |
Foreign References:
| JP2008307007A | 2008-12-25 | |||
| JP2008283972A | 2008-11-27 | |||
| US20080236610A1 | 2008-10-02 | |||
| US20090047263A1 | 2009-02-19 |
Other References:
HORIKIRI, T. ET AL.: "SOX10-Nano-Lantern Reporter Human iPS cells; A Versatile Tool for Neural Crest Research", PLOS ONE, vol. 12, no. 1, 20 January 2017 (2017-01-20), pages 1 - 13, XP55615832
KEROSUO, L. ET AL.: "Crestospheres: Long-Term Maintenance of Multipotent, Premigratory Neural Crest Stem Cells", STEM CELL REPORTS, vol. 5, no. 4, 13 October 2015 (2015-10-13), pages 499 - 507, XP55615836
FUKUTA M. ET AL.: "Derivation of mesenchymal stromal cells from pluripotent stem cells through a neural crest lineage using small molecule compounds with defined media", PLOS ONE, vol. 9, no. 12, 2014, pages e1122 91, XP055332980, DOI: 10.1371/journal.pone.0112291
KEROSUO L. ET AL.: "Crestospheres: Long-Term Maintenance of Multipotent, Premigratory Neural Crest Stem Cells", STEM CELL REPORTS, vol. 5, no. 4, 2015, pages 499 - 507, XP055615836, DOI: 10.1016/j.stemcr.2015.08.017
MENENDEZ L. ET AL.: "Wnt signaling and a Smad pathway blockade direct the differentiation of human pluripotent stem cells to multipotent neural crest cells", PROC. NATL. ACAD. SCI., vol. 108, no. 48, 2011, pages 19240 - 5, XP055112801, DOI: 10.1073/pnas.1113746108
HORIKIRI T. ET AL.: "SOX10-Nano-Lantern Reporter Human iPS Cells; A Versatile Tool for Neural Crest Research", PLOS ONE, vol. 12, no. 1, 2017, pages e0170342, XP055615832, DOI: 10.1371/journal.pone.0170342
TAKAHASHI KYAMANAKA S., CELL, vol. 126, 2006, pages 663 - 676
TAKAHASHI KYAMANAKA S. ET AL., CELL, vol. 131, 2007, pages 861 - 872
OKITA, K.ICHISAKA, T.YAMANAKA, S., NATURE, vol. 451, 2007, pages 141 - 146
HUANGFU D.MELTON, DA. ET AL., NATURE BIOTECHNOLOGY, vol. 26, no. 7, 2008, pages 795 - 797
OKITA K ET AL., NAT. METHODS, vol. 8, no. 5, May 2011 (2011-05-01), pages 409 - 12
OKITA K ET AL., STEM CELLS, vol. 31, no. 3, pages 458 - 66
YU J.THOMSON JA. ET AL., SCIENCE, vol. 318, 2007, pages 1917 - 1920
SHI Y.DING S. ET AL., CELL STEM CELL, vol. 3, no. 5, 2008, pages 568 - 574
KIM JB.SCHOLER HR. ET AL., NATURE, vol. 454, 2008, pages 646 - 650
BETTERS ET AL., DEVELOPMENTAL BIOLOGY, vol. 344, no. 2, 2010, pages 578 - 592
JIANG ET AL., DEVELOPMENT, vol. 127, no. 8, 2000, pages 1607 - 1616
DUPIN ET AL., DEVELOPMENTAL BIOLOGY, vol. 366, no. 1, 2012, pages 83 - 95
NAGOSHI ET AL., CELL STEM CELL, vol. 2, April 2008 (2008-04-01), pages 392 - 403
MOTOHASHI ET AL., BIOLOGY OPEN, vol. 5, 2016, pages 311 - 322
PFALTZGRAFF ET AL., JOURNAL OF VISUALIZED EXPERIMENTS, vol. 64, 2012, pages 4134
SAITO K. ET AL.: "Luminescent proteins for high-speed single-cell and whole-body imaging", NAT. COMMUN., vol. 3, 2012, pages 1262, XP055534182, DOI: 10.1038/ncomms2248
PATSCH ET AL., NATURE CELL BIOLOGY, vol. 17, no. 8, 2015, pages 994 - 1003
UNO ET AL., BRAIN RES., vol. 1296, 2009, pages 148 - 163
See also references of EP 3719120A4
KEROSUO, L. ET AL.: "Crestospheres: Long-Term Maintenance of Multipotent, Premigratory Neural Crest Stem Cells", STEM CELL REPORTS, vol. 5, no. 4, 13 October 2015 (2015-10-13), pages 499 - 507, XP55615836
FUKUTA M. ET AL.: "Derivation of mesenchymal stromal cells from pluripotent stem cells through a neural crest lineage using small molecule compounds with defined media", PLOS ONE, vol. 9, no. 12, 2014, pages e1122 91, XP055332980, DOI: 10.1371/journal.pone.0112291
KEROSUO L. ET AL.: "Crestospheres: Long-Term Maintenance of Multipotent, Premigratory Neural Crest Stem Cells", STEM CELL REPORTS, vol. 5, no. 4, 2015, pages 499 - 507, XP055615836, DOI: 10.1016/j.stemcr.2015.08.017
MENENDEZ L. ET AL.: "Wnt signaling and a Smad pathway blockade direct the differentiation of human pluripotent stem cells to multipotent neural crest cells", PROC. NATL. ACAD. SCI., vol. 108, no. 48, 2011, pages 19240 - 5, XP055112801, DOI: 10.1073/pnas.1113746108
HORIKIRI T. ET AL.: "SOX10-Nano-Lantern Reporter Human iPS Cells; A Versatile Tool for Neural Crest Research", PLOS ONE, vol. 12, no. 1, 2017, pages e0170342, XP055615832, DOI: 10.1371/journal.pone.0170342
TAKAHASHI KYAMANAKA S., CELL, vol. 126, 2006, pages 663 - 676
TAKAHASHI KYAMANAKA S. ET AL., CELL, vol. 131, 2007, pages 861 - 872
OKITA, K.ICHISAKA, T.YAMANAKA, S., NATURE, vol. 451, 2007, pages 141 - 146
HUANGFU D.MELTON, DA. ET AL., NATURE BIOTECHNOLOGY, vol. 26, no. 7, 2008, pages 795 - 797
OKITA K ET AL., NAT. METHODS, vol. 8, no. 5, May 2011 (2011-05-01), pages 409 - 12
OKITA K ET AL., STEM CELLS, vol. 31, no. 3, pages 458 - 66
YU J.THOMSON JA. ET AL., SCIENCE, vol. 318, 2007, pages 1917 - 1920
SHI Y.DING S. ET AL., CELL STEM CELL, vol. 3, no. 5, 2008, pages 568 - 574
KIM JB.SCHOLER HR. ET AL., NATURE, vol. 454, 2008, pages 646 - 650
BETTERS ET AL., DEVELOPMENTAL BIOLOGY, vol. 344, no. 2, 2010, pages 578 - 592
JIANG ET AL., DEVELOPMENT, vol. 127, no. 8, 2000, pages 1607 - 1616
DUPIN ET AL., DEVELOPMENTAL BIOLOGY, vol. 366, no. 1, 2012, pages 83 - 95
NAGOSHI ET AL., CELL STEM CELL, vol. 2, April 2008 (2008-04-01), pages 392 - 403
MOTOHASHI ET AL., BIOLOGY OPEN, vol. 5, 2016, pages 311 - 322
PFALTZGRAFF ET AL., JOURNAL OF VISUALIZED EXPERIMENTS, vol. 64, 2012, pages 4134
SAITO K. ET AL.: "Luminescent proteins for high-speed single-cell and whole-body imaging", NAT. COMMUN., vol. 3, 2012, pages 1262, XP055534182, DOI: 10.1038/ncomms2248
PATSCH ET AL., NATURE CELL BIOLOGY, vol. 17, no. 8, 2015, pages 994 - 1003
UNO ET AL., BRAIN RES., vol. 1296, 2009, pages 148 - 163
See also references of EP 3719120A4
Attorney, Agent or Firm:
OHNO Seiji et al. (JP)
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