Title:
METHOD FOR CULTURING ADHERENT CELLS
Document Type and Number:
WIPO Patent Application WO/2017/104618
Kind Code:
A1
Abstract:
The invention is a method for culturing adherent cells characterized in that adherent cells are cultured in a liquid medium in a culture vessel made of polymer that contains an alicyclic structure, the cells are detached from the culture vessel by a fluid stream without adding a proteolytic enzyme, and the cultured cells are suspended in the liquid medium. The cells can be easily detached by a weak force such as that of pipetting because the cells adhere loosely to the culture vessel made of a polymer that contains an alicyclic structure.
Inventors:
KUSABIRAKI KAZUKI (JP)
ICHIMURA NAOYA (JP)
ICHIMURA NAOYA (JP)
Application Number:
PCT/JP2016/086930
Publication Date:
June 22, 2017
Filing Date:
December 12, 2016
Export Citation:
Assignee:
ZEON CORP (JP)
International Classes:
C12N5/071
Domestic Patent References:
| WO2015093393A1 | 2015-06-25 |
Foreign References:
| JP2009027944A | 2009-02-12 |
Other References:
TAMADA Y. ET AL.: "Effect of preadsorbed proteins on cell adhesion to polymer surfaceS", JOURNAL OF COLLOID AND INTERFACE SCIENCE, vol. 155, 1993, pages 334 - 339, XP055393091, ISSN: 0021-9797
See also references of EP 3392335A4
See also references of EP 3392335A4
Attorney, Agent or Firm:
OHISHI Haruhito (JP)
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