Title:
METHOD FOR CULTURING BACILLUS BACTERIUM, AND METHOD FOR PRODUCING USEFUL SUBSTANCE
Document Type and Number:
WIPO Patent Application WO/2018/066688
Kind Code:
A1
Abstract:
The present invention addresses the problem of providing a culturing method whereby it becomes possible to produce spores of a bacterium belonging to the genus Bacillus with high efficiency. Provided is a method for producing spores of a bacterium belonging to the genus Bacillus, said method being characterized by comprising: culturing the bacterium belonging to the genus Bacillus in a liquid culture medium that contains a sugar or a sugar-derived raw material at a concentration of 50.1 to 100 g/L at the start of the culturing; and culturing the bacterium while supplying a feed culture medium to the liquid culture medium in the middle of the culturing process, wherein the feed culture medium contains a sugar or a sugar-derived raw material and a nitrogenated compound and has a ratio of the amount of carbon atoms to the amount of nitrogen atoms (i.e., a C/N ratio) of 5.5 to 13.5 by weight.
Inventors:
TSUKAGOSHI YUKI (JP)
Application Number:
PCT/JP2017/036440
Publication Date:
April 12, 2018
Filing Date:
October 06, 2017
Export Citation:
Assignee:
IDEMITSU KOSAN CO (JP)
International Classes:
C12N1/20; C12P1/00
Domestic Patent References:
| WO2016163534A1 | 2016-10-13 |
Foreign References:
| JP2010510776A | 2010-04-08 | |||
| JPH11504813A | 1999-05-11 | |||
| JP2003235593A | 2003-08-26 | |||
| JP2007236286A | 2007-09-20 | |||
| JPH11103855A | 1999-04-20 | |||
| JPH06254584A | 1994-09-13 | |||
| JPH09502097A | 1997-03-04 |
Other References:
KANJANACHUMPOL P.: "Enhancing polyhydroxybutyrate production from high cell density fed-batch fermentation of bacillus megaterium BA-019.", BIOPROCESS BIOSYST ENG., vol. 36, no. 10, 2013, pages 1463 - 1474, XP035365252
GAO T . ET AL : "L- lactic acid production by Bacill us subtilis MURl ", BIORESOURCE TECHNOLOGY, vol. 121, 2012, pages 105 - 110, XP055597334
FARRERA R . R . ET AL .,: "Carbon nitrogen ratio interacts with initial concent ration of total solids on insecticidal crystal protein and spore production in Bacillus thuringiensis HD- 73 .", APPL MICROBIOL BIOTECHNOL, vol. 49, no. 6, 1 January 1998 (1998-01-01), pages 758 - 765, XP055321839
ZAIN W. ET AL : "Production of cyclodextrin glucanotransferase from alkalophilic Bacillus sp. TS1-1: Optimization of carbon and nitrogen concentration in the feed medium using central composite design", BIOCHEMICAL ENGINEERING JOURNAL, vol. 33, 1 January 2007 (2007-01-01), pages 26 - 33, XP005809678
ADVANCES IN MICROBIOLOGY, vol. 4, 2014, pages 444 - 454
See also references of EP 3524670A4
GAO T . ET AL : "L- lactic acid production by Bacill us subtilis MURl ", BIORESOURCE TECHNOLOGY, vol. 121, 2012, pages 105 - 110, XP055597334
FARRERA R . R . ET AL .,: "Carbon nitrogen ratio interacts with initial concent ration of total solids on insecticidal crystal protein and spore production in Bacillus thuringiensis HD- 73 .", APPL MICROBIOL BIOTECHNOL, vol. 49, no. 6, 1 January 1998 (1998-01-01), pages 758 - 765, XP055321839
ZAIN W. ET AL : "Production of cyclodextrin glucanotransferase from alkalophilic Bacillus sp. TS1-1: Optimization of carbon and nitrogen concentration in the feed medium using central composite design", BIOCHEMICAL ENGINEERING JOURNAL, vol. 33, 1 January 2007 (2007-01-01), pages 26 - 33, XP005809678
ADVANCES IN MICROBIOLOGY, vol. 4, 2014, pages 444 - 454
See also references of EP 3524670A4
Attorney, Agent or Firm:
SANUKI, Shinichi et al. (JP)
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