Title:
METHOD FOR CULTURING CANCER ORGANOID AND METHOD FOR SCREENING TEST SUBSTANCE
Document Type and Number:
WIPO Patent Application WO/2024/034576
Kind Code:
A1
Abstract:
This method for culturing a cancer organoid includes culturing the cancer organoid on the top surface or inside of a cell structure in a medium free from extracellular matrix, wherein the cell structure contains cells constituting the stroma and two or more cell layers are laminated in the thickness direction therein. The method for screening a test substance includes culturing a cell structure containing a cancer organoid, which is obtained by the aforesaid method for culturing a cancer organoid, in the presence of a test substance, and evaluating the effect of the test substance on the cancer organoid.
Inventors:
MORIMURA RII (JP)
SHINOZAKI EIJI (JP)
KITANO SHIRO (JP)
YAO RYOJI (JP)
MATSUSAKI MICHIYA (JP)
SHINOZAKI EIJI (JP)
KITANO SHIRO (JP)
YAO RYOJI (JP)
MATSUSAKI MICHIYA (JP)
Application Number:
PCT/JP2023/028787
Publication Date:
February 15, 2024
Filing Date:
August 07, 2023
Export Citation:
Assignee:
TOPPAN HOLDINGS INC (JP)
JAPANESE FOUND FOR CANCER RES (JP)
UNIV OSAKA (JP)
JAPANESE FOUND FOR CANCER RES (JP)
UNIV OSAKA (JP)
International Classes:
C12N5/09; C12N1/00; C12Q1/02
Domestic Patent References:
| WO2019039457A1 | 2019-02-28 |
Other References:
NISHIGUCHI AKIHIRO; MATSUSAKI MICHIYA; KANO MITSUNOBU R.; NISHIHARA HIROSHI; OKANO DAISUKE; ASANO YOSHIYA; SHIMODA HIROSHI; KISHIM: "In-vitro 3D blood/lymph-vascularized human stromal tissues for preclinical assays of cancer metastasis", BIOMATERIALS, ELSEVIER, AMSTERDAM, NL, vol. 179, 2 July 2018 (2018-07-02), AMSTERDAM, NL , pages 144 - 155, XP085419779, ISSN: 0142-9612, DOI: 10.1016/j.biomaterials.2018.06.019
LAZZARI GIANPIERO; NICOLAS VALERIE; MATSUSAKI MICHIYA; AKASHI MITSURU; COUVREUR PATRICK; MURA SIMONA: "Multicellular spheroid based on a triple co-culture: A novel 3D model to mimic pancreatic tumor complexity", ACTA BIOMATERIALIA, ELSEVIER, AMSTERDAM, NL, vol. 78, 10 August 2018 (2018-08-10), AMSTERDAM, NL, pages 296 - 307, XP085467140, ISSN: 1742-7061, DOI: 10.1016/j.actbio.2018.08.008
WATANABE SADANORI, YOGO AKITADA, OTSUBO TSUGUTERU, UMEHARA HIROKI, OISHI JUN, KODO TORU, MASUI TOSHIHIKO, TAKAISHI SHIGEO, SENO HI: "Establishment of patient-derived organoids and a characterization-based drug discovery platform for treatment of pancreatic cancer", BMC CANCER, BIOMED CENTRAL, LONDON, GB, vol. 22, no. 1, 1 December 2022 (2022-12-01), LONDON, GB , XP093138157, ISSN: 1471-2407, DOI: 10.1186/s12885-022-09619-9
LAZZARI GIANPIERO; NICOLAS VALERIE; MATSUSAKI MICHIYA; AKASHI MITSURU; COUVREUR PATRICK; MURA SIMONA: "Multicellular spheroid based on a triple co-culture: A novel 3D model to mimic pancreatic tumor complexity", ACTA BIOMATERIALIA, ELSEVIER, AMSTERDAM, NL, vol. 78, 10 August 2018 (2018-08-10), AMSTERDAM, NL, pages 296 - 307, XP085467140, ISSN: 1742-7061, DOI: 10.1016/j.actbio.2018.08.008
WATANABE SADANORI, YOGO AKITADA, OTSUBO TSUGUTERU, UMEHARA HIROKI, OISHI JUN, KODO TORU, MASUI TOSHIHIKO, TAKAISHI SHIGEO, SENO HI: "Establishment of patient-derived organoids and a characterization-based drug discovery platform for treatment of pancreatic cancer", BMC CANCER, BIOMED CENTRAL, LONDON, GB, vol. 22, no. 1, 1 December 2022 (2022-12-01), LONDON, GB , XP093138157, ISSN: 1471-2407, DOI: 10.1186/s12885-022-09619-9
Attorney, Agent or Firm:
MATSUNUMA Yasushi et al. (JP)
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