METHOD FOR CULTURING INFLUENZA VIRUS - UNIV OSAKA RES FOUND FOR MICROBIAL DISEASES

Title:

METHOD FOR CULTURING INFLUENZA VIRUS

Document Type and Number:

WIPO Patent Application WO/2020/004425

Kind Code:

A1

Abstract:

Provided is a method for culturing the influenza virus using MDCK cells as a host, wherein the influenza virus is cultured more efficiently. The influenza virus is inoculated under protease-free medium conditions after the MDCK cells have passed the logarithmic growth phase. Aggregation and damage of MDCK cells by protease can be prevented, and the influenza virus can be cultured efficiently. In addition, a higher yield of viral protein is expected than when the influenza virus is grown using chicken eggs as the host.

Inventors:

HORIKOSHI FUMIAKI (JP)
FUJIMOTO TAKAO (JP)
UOTANI TAE (JP)
SHIGYO KOUHEI (JP)
YAMAGUCHI YOSHIYUKI (JP)
NISHIYAMA NAGISA (JP)
NISHIHARA SHUNYA (JP)
TACHIKAWA RYO (JP)
FUJITA TAKESHI (JP)
MAEKAWA YUKIE (JP)

Application Number:

PCT/JP2019/025252

Publication Date:

January 02, 2020

Filing Date:

June 25, 2019

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Assignee:

UNIV OSAKA RES FOUND FOR MICROBIAL DISEASES (JP)

International Classes:

C12N7/00; C12N5/10

Domestic Patent References:

WO2017170592A1

2017-10-05

Foreign References:

JP2007537760A	2007-12-27
JP2008546408A	2008-12-25
JPH10503093A	1998-03-24

Other References:

NOMA, K. ET AL.: "Endogenous protease-dependent replication of human influenza viruses in two MDCK cell lines", ARCHIVES OF VIROLOGY, vol. 143, 1998, pages 1893 - 1909, XP002980321
KESSLER, N. ET AL.: "Suitability of MDCK cells grown in a serum-free medium for influenza virus production", DEVELOPMENTS IN BIOLOGICAL STANDARDIZATION, vol. 98, 1999, pages 13 - 21

Attorney, Agent or Firm:

SHOJI, Takashi et al. (JP)

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