Title:
METHOD FOR CULTURING PLURIPOTENT STEM CELLS
Document Type and Number:
WIPO Patent Application WO/2016/052657
Kind Code:
A1
Abstract:
Provided is a method for efficiently culturing pluripotent stem cells at a higher safety. The present invention relates to: a method for culturing pluripotent stem cells, said method comprising culturing isolated pluripotent stem cells in a quasi-microgravity environment, thus proliferating the pluripotent stem cells while retaining the pluripotent stem cells in undifferentiated conditions, and thereby forming and developing spheroids of the pluripotent stem cells; and a method for inducing differentiation of pluripotent stem cells using the aforesaid method.
Inventors:
UEMURA TOSHIMASA (JP)
ONOMURA YUI (JP)
TSUMURA TAKASHI (JP)
ONOMURA YUI (JP)
TSUMURA TAKASHI (JP)
Application Number:
PCT/JP2015/077824
Publication Date:
April 07, 2016
Filing Date:
September 30, 2015
Export Citation:
Assignee:
NAT INST OF ADVANCED IND SCIEN (JP)
JTEC CORP (JP)
JTEC CORP (JP)
International Classes:
C12N5/10; C12N5/0735; C12N5/074
Domestic Patent References:
| WO2014115799A1 | 2014-07-31 | |||
| WO2010143651A1 | 2010-12-16 |
Foreign References:
| JP2008099662A | 2008-05-01 |
Other References:
YUMI KAWAHARA ET AL.: "Bisho Juryoku Kankyo o Riyo shita Tanosei Kansaibo Baiyo no Kokoromi", REGENERATIVE MEDICINE, vol. 13, February 2014 (2014-02-01), pages 304, XP009501507
LOUIS YUGE: "Development of stem cells culture in simulated microgravity environment", THE JAPAN SOCIETY OF MECHANICAL ENGINEERS DAI 24 KAI BIOENGINEERING KOENKAI RONBUNSHU, 2012, pages 7C14, XP009501513
YUMI KAWAHARA ET AL.: "Bisho Juryoku Kankyo to Kansaibo no Mibunka Iji -Mouse ES Saibo deno Kento", REGENERATIVE MEDICINE, vol. 9, 2010, pages 269, XP009502105
KYOSUKE NAKATA ET AL.: "The Effect of Microgravity Using a Three Dimensional Clinostat on the Chondrogenic Differentiation of Human Mesenchymal Stem Cells", RIGAKU RYOHOGAKU, vol. 41, no. 2, April 2014 (2014-04-01), pages 90 - 91, XP055424005, ISSN: 0289-3770
BLABER E.A. ET AL.: "Mechanical unloading of bone in microgravity reduces mesenchymal and hematopoietic stem cell -mediated tissue regeneration", STEM CELL RES., vol. 13, no. 2, June 2014 (2014-06-01), pages 181 - 201, XP029061737, ISSN: 1873-5061, DOI: doi:10.1016/j.scr.2014.05.005
SHIN'YA YAMAZAKI ET AL.: "The rotating culture system regulates the osteogenic fate of human bone mesenchymal stem cells", SEIKATSU SEIMEI SHIEN IRYO FUKUSHI KOGAKUKEI GAKKAI RENGO TAIKAI KOEN RONBUNSHU, vol. 2010, no. 2A2-3, 2010, pages 169, XP009501523
WANG NANDING ET AL.: "The simulated microgravity enhances multipotential differentiation capacity of bone marrow mesenchymal stem cells", CYTOTECHNOLOGY, vol. 66, no. 1, January 2014 (2014-01-01), pages 119 - 131, XP009501521, ISSN: 0920-9069
TAKESHI IMURA ET AL.: "Mogi Bisho Juryoku Kankyoka deno Saibokai Keisei to Shinkei Bunkano", REGENERATIVE MEDICINE, vol. 9, 2010, pages 264, XP009502103
MORIO UENO: "Marked improvement of human ES cell culture by the ROCK inhibitor Y-27632", JOURNAL OF CLINICAL AND EXPERIMENTAL MEDICINE, vol. 227, no. 4, 2008, pages 254 - 258, XP009501496, ISSN: 0039-2359
TOSHIMASA UEMURA ET AL.: "RWV Kaiten Bioreactor no Jidoka System to Soshiki Saisei eno Oyo", REGENERATIVE MEDICINE, vol. 8, 2009, pages 183, XP009501503
TOSHIMASA UEMURA: "RWV Bioreactor ni yoru Gibisho Juryoku Baiyo o Mochiita iPS Saibo no Tairyo Baiyoho no Kaihatsu", DAI 36 KAI THE ANNUAL MEETING OF THE JAPANESE SOCIETY FOR BIOMATERIALS YOKOSHU, November 2014 (2014-11-01), pages 76, XP009501500
TOSHIMASA UEMURA: "RWV Bioreactor o Mochiita Sanjigen Baiyo ni yoru iPS Saibo no Tairyo Baiyo", THE JAPANESE SOCIETY FOR ALTERNATIVES TO ANIMAL EXPERIMENTS DAI 27 KAI TAIKAI PROGRAM/KOEN YOSHISHU, November 2014 (2014-11-01), pages 136, XP009501504
See also references of EP 3202896A4
LOUIS YUGE: "Development of stem cells culture in simulated microgravity environment", THE JAPAN SOCIETY OF MECHANICAL ENGINEERS DAI 24 KAI BIOENGINEERING KOENKAI RONBUNSHU, 2012, pages 7C14, XP009501513
YUMI KAWAHARA ET AL.: "Bisho Juryoku Kankyo to Kansaibo no Mibunka Iji -Mouse ES Saibo deno Kento", REGENERATIVE MEDICINE, vol. 9, 2010, pages 269, XP009502105
KYOSUKE NAKATA ET AL.: "The Effect of Microgravity Using a Three Dimensional Clinostat on the Chondrogenic Differentiation of Human Mesenchymal Stem Cells", RIGAKU RYOHOGAKU, vol. 41, no. 2, April 2014 (2014-04-01), pages 90 - 91, XP055424005, ISSN: 0289-3770
BLABER E.A. ET AL.: "Mechanical unloading of bone in microgravity reduces mesenchymal and hematopoietic stem cell -mediated tissue regeneration", STEM CELL RES., vol. 13, no. 2, June 2014 (2014-06-01), pages 181 - 201, XP029061737, ISSN: 1873-5061, DOI: doi:10.1016/j.scr.2014.05.005
SHIN'YA YAMAZAKI ET AL.: "The rotating culture system regulates the osteogenic fate of human bone mesenchymal stem cells", SEIKATSU SEIMEI SHIEN IRYO FUKUSHI KOGAKUKEI GAKKAI RENGO TAIKAI KOEN RONBUNSHU, vol. 2010, no. 2A2-3, 2010, pages 169, XP009501523
WANG NANDING ET AL.: "The simulated microgravity enhances multipotential differentiation capacity of bone marrow mesenchymal stem cells", CYTOTECHNOLOGY, vol. 66, no. 1, January 2014 (2014-01-01), pages 119 - 131, XP009501521, ISSN: 0920-9069
TAKESHI IMURA ET AL.: "Mogi Bisho Juryoku Kankyoka deno Saibokai Keisei to Shinkei Bunkano", REGENERATIVE MEDICINE, vol. 9, 2010, pages 264, XP009502103
MORIO UENO: "Marked improvement of human ES cell culture by the ROCK inhibitor Y-27632", JOURNAL OF CLINICAL AND EXPERIMENTAL MEDICINE, vol. 227, no. 4, 2008, pages 254 - 258, XP009501496, ISSN: 0039-2359
TOSHIMASA UEMURA ET AL.: "RWV Kaiten Bioreactor no Jidoka System to Soshiki Saisei eno Oyo", REGENERATIVE MEDICINE, vol. 8, 2009, pages 183, XP009501503
TOSHIMASA UEMURA: "RWV Bioreactor ni yoru Gibisho Juryoku Baiyo o Mochiita iPS Saibo no Tairyo Baiyoho no Kaihatsu", DAI 36 KAI THE ANNUAL MEETING OF THE JAPANESE SOCIETY FOR BIOMATERIALS YOKOSHU, November 2014 (2014-11-01), pages 76, XP009501500
TOSHIMASA UEMURA: "RWV Bioreactor o Mochiita Sanjigen Baiyo ni yoru iPS Saibo no Tairyo Baiyo", THE JAPANESE SOCIETY FOR ALTERNATIVES TO ANIMAL EXPERIMENTS DAI 27 KAI TAIKAI PROGRAM/KOEN YOSHISHU, November 2014 (2014-11-01), pages 136, XP009501504
See also references of EP 3202896A4
Attorney, Agent or Firm:
HIRAKI Yusuke et al. (JP)
Yusuke Hiraki (JP)
Yusuke Hiraki (JP)
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