Title:
METHOD OF CULTURING VASCULAR SMOOTH MUSCLE CELLS, CULTURE DEVICE AND MEDICAL MATERIAL OBTAINED BY THE CULTURE
Document Type and Number:
WIPO Patent Application WO/2007/138973
Kind Code:
A1
Abstract:
It is intended to provide a method of culturing vascular smooth muscle cells while sustaining the normal function of the cells, a culture device therefor, and a material for regenerative medicine. In this method, use is made of the fact that vascular smooth muscle cells recognize elastin as an extracellular matrix and grow using the same. Namely, a method of culturing vascular smooth muscle cells on elastin; a culture device in which elastin is fixed on the surface where cells grow; a culture device in which the surface where cells grow comprises a molded article made of elastin; and a medical material which is obtained by culturing vascular smooth muscle cells by using such a culture device.
Inventors:
MIYAMOTO KEIICHI (JP)
HORIUCHI TAKASHI (JP)
HORIUCHI TAKASHI (JP)
Application Number:
PCT/JP2007/060604
Publication Date:
December 06, 2007
Filing Date:
May 24, 2007
Export Citation:
Assignee:
CHISSO CORP (JP)
MIYAMOTO KEIICHI (JP)
HORIUCHI TAKASHI (JP)
MIYAMOTO KEIICHI (JP)
HORIUCHI TAKASHI (JP)
International Classes:
C12N5/00; A61F2/06; A61L27/00; A61L27/38; A61L27/50; C12M3/00; C12N5/02; C12N5/07; C12N5/071; C12N5/077
Domestic Patent References:
| WO2002096978A1 | 2002-12-05 | |||
| WO2002096978A1 | 2002-12-05 |
Foreign References:
| JP2003189843A | 2003-07-08 |
Other References:
YAMAMOTO K. ET AL.: "Role of Elastin in the Differentiated Properties of Vascular Smooth Muscle Cells in Culture", CONNECTIVE TISSUE, vol. 26, no. 3, 1994, pages 245 - 254, XP003019387
ENGBERS-BUIJTENHUIJS P. ET AL.: "Biological characterization of vascular grafts cultured in a bioreactor", BIOMATERIALS, vol. 27, no. 11, April 2006 (2006-04-01), pages 1 - 6, XP005223296
KITAZONO E. ET AL.: "Tissue Engineering using Nanofiber", JOURNAL OF SYNTHETIC ORGANIC CHEMISTRY, JAPAN, vol. 62, no. 5, 2004, pages 514 - 519, XP003019388
CHO M. ET AL.: "Phenotypic changes of vascular smooth muscle cells in three dimensional collagen gel for the development of vascular graft was composed of biomaterials", THE JAPANESE JOURNAL OF ARTIFICIAL ORGANS, vol. 26, no. 5, 1997, pages 946 - 951, XP003019389
See also references of EP 2020435A4
K. NARUSE; T. YAMADA; M. SOKABE, AM. J. PHYSIOL., vol. 274, 1998, pages H1532 - 1538
MIYAMOTO ET AL., INT. BIOL. MACROMOL., vol. 30, 2002, pages 75 - 80
ENGBERS-BUIJTENHUIJS P. ET AL.: "Biological characterization of vascular grafts cultured in a bioreactor", BIOMATERIALS, vol. 27, no. 11, April 2006 (2006-04-01), pages 1 - 6, XP005223296
KITAZONO E. ET AL.: "Tissue Engineering using Nanofiber", JOURNAL OF SYNTHETIC ORGANIC CHEMISTRY, JAPAN, vol. 62, no. 5, 2004, pages 514 - 519, XP003019388
CHO M. ET AL.: "Phenotypic changes of vascular smooth muscle cells in three dimensional collagen gel for the development of vascular graft was composed of biomaterials", THE JAPANESE JOURNAL OF ARTIFICIAL ORGANS, vol. 26, no. 5, 1997, pages 946 - 951, XP003019389
See also references of EP 2020435A4
K. NARUSE; T. YAMADA; M. SOKABE, AM. J. PHYSIOL., vol. 274, 1998, pages H1532 - 1538
MIYAMOTO ET AL., INT. BIOL. MACROMOL., vol. 30, 2002, pages 75 - 80
Attorney, Agent or Firm:
TAKAGI, Chiyoshi et al. (Kojimachi Koyo Bldg.10, Kojimachi 1-chome,Chiyoda-k, Tokyo 83, JP)
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