Title:
METHOD FOR DESIGNING MUTANT ENZYME, METHOD FOR PREPARING MUTANT ENZYME, AND MUTANT ENZYME
Document Type and Number:
WIPO Patent Application WO/2010/029685
Kind Code:
A1
Abstract:
Disclosed is a novel method for improving an enzyme capable of deamidating a protein. A mutant enzyme can be designed by the following steps (1) and (2): (1) specifying at least one amino acid residue among amino acid residues corresponding to the amino acid residues located at position-35, position-38, position-39, position-40, position-41, position-42, position-43, position-45, position-46, position-49, position-79, position-80, position-81, position-82, position-83, position-84, position-103, position-104, position-105, position-106, position-117, position-142, position-143, position-146, position-166 and position-185 in the amino acid sequence depicted in SEQ ID NO:2 which is an amino acid sequence for a protein deamidase (an enzyme to be mutated); and (2) constructing an amino acid sequence having the substitution of the amino acid residue specified in step (1) by another amino acid residue or having the deletion of the amino acid residue specified in step (1) by using the amino acid sequence for the enzyme to be mutated as a base sequence.
Inventors:
HASHIZUME RYOTA (JP)
MIKAMI BUNZO (JP)
MATSUBARA HIROTAKA (JP)
MATSUNAGA AKIKO (JP)
YAMAGUCHI SHOTARO (JP)
MIKAMI BUNZO (JP)
MATSUBARA HIROTAKA (JP)
MATSUNAGA AKIKO (JP)
YAMAGUCHI SHOTARO (JP)
Application Number:
PCT/JP2009/003870
Publication Date:
March 18, 2010
Filing Date:
August 12, 2009
Export Citation:
Assignee:
AMANO ENZYME INC (JP)
HASHIZUME RYOTA (JP)
MIKAMI BUNZO (JP)
MATSUBARA HIROTAKA (JP)
MATSUNAGA AKIKO (JP)
YAMAGUCHI SHOTARO (JP)
HASHIZUME RYOTA (JP)
MIKAMI BUNZO (JP)
MATSUBARA HIROTAKA (JP)
MATSUNAGA AKIKO (JP)
YAMAGUCHI SHOTARO (JP)
International Classes:
C12N15/09; C12N1/15; C12N1/19; C12N1/21; C12N5/10; C12N9/00; C12P21/02
Domestic Patent References:
| WO2008138900A2 | 2008-11-20 |
Foreign References:
| JP2000050887A | 2000-02-22 | |||
| JP2001218590A | 2001-08-14 | |||
| JP2000050887A | 2000-02-22 | |||
| JP2001218590A | 2001-08-14 | |||
| JP2004097099A | 2004-04-02 |
Other References:
VAINTRAUB, I.A., KOTOVA, L.V., SHAHA, R.: "Protein deamidases from germinating seeds", PHYSIOL. PLANTARUM., vol. 96, 1996, pages 662 - 666
TAKAYUKI UWAJIMA: "Industrial Enzymes", 1995, MARUZEN CO., LTD.
KIKUCHI, M., HAYASHIDA, H., NAKANO, E., SAKAGUCHI K.: "Peptidoglutaminase. Enzymes for selective deamidation of y-amido of peptide-bound glutamine", BIOCHEMISTRY, vol. 10, 1971, pages 1222 - 1229, XP000990230, DOI: doi:10.1021/bi00783a019
KARLIN, ALTSCHUL, PROC. NATL. ACAD. SCI. USA, vol. 87, 1990, pages 2264 - 68
KARLIN, ALTSCHUL, PROC. NATL. ACAD. SCI. USA, vol. 90, 1993, pages 5873 - 77
ALTSCHUL ET AL., J. MOL. BIOL., vol. 215, 1990, pages 403 - 10
ALTSCHUL ET AL., AMINO ACIDS RESEARCH, vol. 25, no. 17, 1997, pages 3389 - 3402
MYERS, MILLER, COMPUT APPL BIOSCI., vol. 4, 1988, pages 11 - 17
"Molecular Cloning", COLD SPRING HARBOR LABORATORY PRESS
J. MOL. BIOL., vol. 331, 2003, pages 585 - 592
NUCLEIC ACID RES., vol. 16, 1988, pages 7351 - 7367
SHIMIZU, Y. ET AL., NATURE BIOTECH., vol. 19, 2001, pages 751 - 755
"Current protocols in molecular biology", 1987
J. MOL. BIOL., vol. 53, 1970, pages 159
J. MOL. BIOL., vol. 166, 1983, pages 557
GENE, vol. 96, 1990, pages 23
CHUNG ET AL., PROC. NATL. ACAD. SCI. U.S.A., vol. 86, 1989, pages 2172
POTTER, H. ET AL., PROC. NATL. ACAD. SCI. U.S.A., vol. 81, 1984, pages 7161 - 7165
FELGNER, P.L. ET AL., PROC. NATL. ACAD. SCI. U.S.A., vol. 84, 1984, pages 7413 - 7417
YAMAGUCHI, S., JEENS D.J., ARCHER, D.B.: "Protein-glutaminase from Chryseobacterium proteolyticum, an enzyme that deamidates glutaminyl residues in proteins. Purification, characterization and gene cloning", EUR. J. BIOCHEM., vol. 268, 2001, pages 1410 - 1421, XP055044783, DOI: doi:10.1046/j.1432-1327.2001.02019.x
SAMBROOK ET AL.: "Molecular Cloning: a laboratory manual", 1989, COLD SPRING HARBOR LABORATORY PRESS
TAKAYUKI UWAJIMA: "Industrial Enzymes", 1995, MARUZEN CO., LTD.
KIKUCHI, M., HAYASHIDA, H., NAKANO, E., SAKAGUCHI K.: "Peptidoglutaminase. Enzymes for selective deamidation of y-amido of peptide-bound glutamine", BIOCHEMISTRY, vol. 10, 1971, pages 1222 - 1229, XP000990230, DOI: doi:10.1021/bi00783a019
KARLIN, ALTSCHUL, PROC. NATL. ACAD. SCI. USA, vol. 87, 1990, pages 2264 - 68
KARLIN, ALTSCHUL, PROC. NATL. ACAD. SCI. USA, vol. 90, 1993, pages 5873 - 77
ALTSCHUL ET AL., J. MOL. BIOL., vol. 215, 1990, pages 403 - 10
ALTSCHUL ET AL., AMINO ACIDS RESEARCH, vol. 25, no. 17, 1997, pages 3389 - 3402
MYERS, MILLER, COMPUT APPL BIOSCI., vol. 4, 1988, pages 11 - 17
"Molecular Cloning", COLD SPRING HARBOR LABORATORY PRESS
J. MOL. BIOL., vol. 331, 2003, pages 585 - 592
NUCLEIC ACID RES., vol. 16, 1988, pages 7351 - 7367
SHIMIZU, Y. ET AL., NATURE BIOTECH., vol. 19, 2001, pages 751 - 755
"Current protocols in molecular biology", 1987
J. MOL. BIOL., vol. 53, 1970, pages 159
J. MOL. BIOL., vol. 166, 1983, pages 557
GENE, vol. 96, 1990, pages 23
CHUNG ET AL., PROC. NATL. ACAD. SCI. U.S.A., vol. 86, 1989, pages 2172
POTTER, H. ET AL., PROC. NATL. ACAD. SCI. U.S.A., vol. 81, 1984, pages 7161 - 7165
FELGNER, P.L. ET AL., PROC. NATL. ACAD. SCI. U.S.A., vol. 84, 1984, pages 7413 - 7417
YAMAGUCHI, S., JEENS D.J., ARCHER, D.B.: "Protein-glutaminase from Chryseobacterium proteolyticum, an enzyme that deamidates glutaminyl residues in proteins. Purification, characterization and gene cloning", EUR. J. BIOCHEM., vol. 268, 2001, pages 1410 - 1421, XP055044783, DOI: doi:10.1046/j.1432-1327.2001.02019.x
SAMBROOK ET AL.: "Molecular Cloning: a laboratory manual", 1989, COLD SPRING HARBOR LABORATORY PRESS
Attorney, Agent or Firm:
HAGINO, Mikiharu (JP)
Kanji Hagino (JP)
Kanji Hagino (JP)
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