Title:
METHOD OF DETECTING BINDING SITE TO DNA-BINDING PROTEIN IN DNA
Document Type and Number:
WIPO Patent Application WO/2008/029667
Kind Code:
A1
Abstract:
It is intended to provide a method whereby a region containing a binding site of a
DNA capable of binding to a DNA-binding protein in the DNA can be conveniently and
quickly detected. This method is characterized by comprising: preparing a
first liquid mixture containing a fluorescent labeled DNA-binding protein and
a first DNA known as being bound by the above protein and a second liquid mixture containing
a second DNA and a fluorescent labeled DNA-binding protein; examining the presence
or absence of a difference in Brownian motion speed between the DNA-binding protein
in the first liquid mixture and the DNA-binding protein in the second liquid mixture
based on the fluorescence intensities of the first and second liquid mixtures;
and detecting the region containing the binding site of the DNA based on the results.
To detect the Brownian motion speeds of the DNA-binding proteins, use may be
made of the fluorescence correlation spectrometry, the fluorescence depolarization
method or the like.
Inventors:
GOHDA, Kazuhito (43-2 Hatagaya 2-chom, Shibuya-ku Tokyo 72, 1510072, JP)
Application Number:
JP2007/066622
Publication Date:
March 13, 2008
Filing Date:
August 28, 2007
Export Citation:
Assignee:
OLYMPUS CORPORATION (43-2, Hatagaya 2-chome Shibuya-ku Tokyo, 72, 1510072, JP)
オリンパス株式会社 (〒72 東京都渋谷区幡ヶ谷２丁目４３番２号 Tokyo, 1510072, JP)
オリンパス株式会社 (〒72 東京都渋谷区幡ヶ谷２丁目４３番２号 Tokyo, 1510072, JP)
International Classes:
C12Q1/68; C12N15/09; C12Q1/34; G01N21/78; C12Q1/68; C12N15/09; C12Q1/34; G01N21/77
Attorney, Agent or Firm:
AKASHI, Masaki et al. (5F YH Bldg, 6-8 Shinkawa 2-chome, Chuo-ku Tokyo 33, 1040033, JP)
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