Title:
METHOD FOR DETECTING DETECTION TARGET WITHIN SUBJECT BODY, DETECTION KIT, AND FLUID DEVICE
Document Type and Number:
WIPO Patent Application WO/2019/069371
Kind Code:
A1
Abstract:
Provided is a method for detecting a detection target with a subject body, wherein magnetic particles are used to detect the detection target within the subject body. This method for detecting a detection target within a subject body is characterized by including: (a) a step in which the subject body is brought into contact with magnetic particles to which a nucleic acid and a detection target-binding substance that specifically binds to the detection target within the subject body are anchored, and a first complex including the magnetic particles and the detection target is produced; (b) a step in which a solid-phase carrier (hereinafter, nucleic acid probe anchoring carrier) to which a nucleic acid probe that specifically binds to the nucleic acid is anchored, the first complex, and a labeling substance in which a substance that specifically binds with the detection target is labeled are brought into contact, and a second complex including the nucleic acid probe, the first complex, and the labeling substance is produced; and (c) a step in which the detection target is detected by detecting the label of the labeling substance of the second complex, which is bound to the nucleic acid probe anchoring carrier.
Inventors:
SUZUKI KUNO (JP)
SHIONO HIROFUMI (JP)
UENO TARO (JP)
SHIONO HIROFUMI (JP)
UENO TARO (JP)
Application Number:
PCT/JP2017/035992
Publication Date:
April 11, 2019
Filing Date:
October 03, 2017
Export Citation:
Assignee:
NIKON CORP (JP)
International Classes:
G01N33/543; C12Q1/68; G01N33/553
Domestic Patent References:
| WO2013051651A1 | 2013-04-11 | |||
| WO2016081740A1 | 2016-05-26 | |||
| WO2008139419A1 | 2008-11-20 |
Foreign References:
| JP2007525970A | 2007-09-13 | |||
| JP2006501486A | 2006-01-12 | |||
| JP2007514932A | 2007-06-07 |
Attorney, Agent or Firm:
NISHIZAWA Kazuyoshi et al. (JP)
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