Title:
METHOD FOR DETECTING GUANINE-ABASIC SITE IN DNA
Document Type and Number:
WIPO Patent Application WO/2015/136999
Kind Code:
A1
Abstract:
The present invention provides a method for detecting the presence or absence of guanine-abasic sites, the method being a process for detecting guanines opposite abasic sites that occur in double stranded DNA. The method comprises: (1) a first step for position-selectively cutting an abasic site in double stranded DNA using oxygen; (2) a second step for modifying the 2-position amino group of a guanine that is opposite to an abasic site using a modifier; and (3) a third step for performing a polymerase chain reaction having the modified double stranded DNA obtained by performing the first and second steps as a template and checking for the presence or absence of amplification products. (The order of the first step and the second step does not matter.)
More Like This:
Inventors:
WU CHUN (JP)
Application Number:
PCT/JP2015/052475
Publication Date:
September 17, 2015
Filing Date:
January 29, 2015
Export Citation:
Assignee:
NAT INST OF ADVANCED IND SCIEN (JP)
International Classes:
C12Q1/68; G01N33/50; C12N15/09
Foreign References:
| JP2004049182A | 2004-02-19 | |||
| JP2012223203A | 2012-11-15 | |||
| JP2006172025A | 2006-06-29 |
Other References:
BUI CHINH T. ET AL.: "Site-selective reactions of imperfectly matched DNA with small chemical molecules: applications in mutation detection", BIOORGANIC CHEMISTRY, vol. 30, 2002, pages 216 - 232, XP009042654
SATO YUSUKE ET AL.: "Competitive Binding of Abasic Site-Binding Ligands and Masking Ligands to DNA Duplexes for the Analysis of Single-Base Mutation", ANALYTICAL SCIENCES, vol. 29, 2013, pages 15 - 19, XP055223903
SUN HUI BIN ET AL.: "Detection of Abasic Sites on Individual DNA Molecules Using Atomic Force Microscopy", ANALYTICAL CHEMISTRY, vol. 73, no. 10, 2001, pages 2229 - 2232, XP055223904
SATO YUSUKE ET AL.: "Competitive Binding of Abasic Site-Binding Ligands and Masking Ligands to DNA Duplexes for the Analysis of Single-Base Mutation", ANALYTICAL SCIENCES, vol. 29, 2013, pages 15 - 19, XP055223903
SUN HUI BIN ET AL.: "Detection of Abasic Sites on Individual DNA Molecules Using Atomic Force Microscopy", ANALYTICAL CHEMISTRY, vol. 73, no. 10, 2001, pages 2229 - 2232, XP055223904
Attorney, Agent or Firm:
SAEGUSA & PARTNERS (JP)
Patent business corporation 3 Edakuni [Hajime] patent firm (JP)
Patent business corporation 3 Edakuni [Hajime] patent firm (JP)
Download PDF:
Previous Patent: MULTI-CHIP MODULE, ON-BOARD COMPUTER, SENSOR INTERFACE SUBSTRATE, AND MULTI-CHIP MODULE MANUFACTURIN...
Next Patent: CHILLING MACHINE
Next Patent: CHILLING MACHINE