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Title:
METHOD FOR DETECTING HUMAN GENOMIC DNA
Document Type and Number:
WIPO Patent Application WO/2018/101375
Kind Code:
A1
Abstract:
Provided is a method for detecting and/or assaying human genomic DNA in a test sample at high sensitivity. One "human Alu model sequence" representing the consensus sequence of 46 human Alu subfamilies is identified, and PCR primer-probe candidates for Alu detection are selected using this human Alu model sequence. Next, a primer-probe set that is an oligonucleotide sequence which hybridizes specifically with the human Alu sequence and that comprises an oligonucleotide sequence capable of minimizing dimer formation is selected using proprietary criteria. The human genomic DNA in the test sample can be detected and/or assayed at high sensitivity by conducting quantitative real-time PCR using the PCR primer-probe for Alu detection obtained in this manner.

Inventors:
AKASHI HIDEO (JP)
FUNAKOSHI KODAI (JP)
Application Number:
JP2017/042943
Publication Date:
June 07, 2018
Filing Date:
November 30, 2017
Export Citation:
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Assignee:
UNIV AKITA (JP)
International Classes:
C12Q1/68; C12N15/09
Domestic Patent References:
WO2005017176A22005-02-24
Foreign References:
JP2005524384A2005-08-18
JP2008545418A2008-12-18
US20040102395A12004-05-27
US20040014051A12004-01-22
US20140057262A12014-02-27
CN1904900A2007-01-31
Other References:
FUNAKOSHI K. ET AL.: "Highly sensitive and specific Alu-based quantification of human cells among rodent cells", SCIENTIFIC REPORTS, vol. 7, no. 1, 16 October 2017 (2017-10-16), XP055510120
Attorney, Agent or Firm:
HIROTA, Masanori (JP)
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