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Patent Searching and Data


Title:
METHOD FOR DETECTING HYDROGEN PEROXIDE CONVERSION ACTIVITY OF AN ENZYME
Document Type and Number:
WIPO Patent Application WO/2019/052907
Kind Code:
A3
Abstract:
The present invention relates to the detection of enzyme activity, in particular hydrogen conversion activity of an enzyme such as peroxygenase or peroxidase activity. More particularly, the invention provides a microplate-scale absorbance/fluorescence-based method for the detection of hydrogen conversion activity of an enzyme based on the measurement of hydrogen peroxide changes. The invention further provides screening methods for enzymes with improved hydrogen conversion activity, and a kit and combination of reagents suitable for use in the methods of the invention.

Inventors:
FOURAGE LAURENT (FR)
LI SHENGYING (CN)
XU HUIFANG (CN)
NING LINLIN (CN)
Application Number:
PCT/EP2018/074109
Publication Date:
June 06, 2019
Filing Date:
September 07, 2018
Export Citation:
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Assignee:
TOTAL RAFFINAGE CHIMIE (FR)
QINGDAO INSTITUTE OF BIOENERGY AND BIOPROCESS TECH QIBEBT (CN)
International Classes:
C12Q1/28; C10G3/00; C12Q1/30; G01N33/50
Domestic Patent References:
WO2005017106A22005-02-24
Other References:
TADAYUKI IWASE ET AL: "A Simple Assay for Measuring Catalase Activity: A Visual Approach", SCIENTIFIC REPORTS, vol. 3, no. 1, 30 October 2013 (2013-10-30), XP055438230, DOI: 10.1038/srep03081
JOB L. GRANT ET AL: "Decarboxylation of Fatty Acids to Terminal Alkenes by Cytochrome P450 Compound I", JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, vol. 137, no. 15, 22 April 2015 (2015-04-22), pages 4940 - 4943, XP055206222, ISSN: 0002-7863, DOI: 10.1021/jacs.5b01965
Attorney, Agent or Firm:
DE CLERCQ & PARTNERS (BE)
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