METHOD FOR DETECTING INTERCELLULAR INTERACTION AND USE THEREOF

WIPO Patent Application WO/2024/092740

A1

Provided is a method for detecting intercellular interaction. The method comprises: (1) preparing a first cell expressing a TCR activation response element and a reporter molecule, the first cell expressing a TCR; (2) co-incubating an antigen peptide with a second cell expressing an MHC to give a second cell loaded with the antigen peptide; (3) marking the first cell using a first label to give a first cell carrying the label, and marking the second cell loaded with the antigen peptide using a second label to give a second cell carrying the label; (4) encapsulating the first cell carrying the label, the second cell carrying the label, and a magnetic bead using an oil phase to prepare a plurality of liquid droplets; (5) injecting a cell lysis buffer into a single liquid droplet, and sequencing nucleic acids after lysis; and (6) determining, according to the sequencing result, whether the first cell and the second cell in the single liquid droplet have interaction with each other.

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LIU YA (CN)
QI JINGYU (CN)
GU YING (CN)
LIU YANG (CN)
LI YIJIAN (CN)
WU JING (CN)
DONG XUAN (CN)
LIU LONGQI (CN)

PCT/CN2022/129973

May 10, 2024

November 04, 2022

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BGI SHENZHEN (CN)

A61P35/00; C07K14/725; C12N15/10; C12Q1/6869; G01N33/566

IP MARCH (Floor 15 Satellite Building, No. 63 Zhichun Road, Haidian District, Beijing 0, CN)

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