Title:
METHOD FOR DETECTING MUTANT DNA
Document Type and Number:
WIPO Patent Application WO/2012/039481
Kind Code:
A1
Abstract:
The present invention relates to a method for detecting mutant DNA using a probe, and is characterized in comprising the steps of: (1) bringing into contact a sample containing single-stranded DNA (mutant DNA) having a substituted base, a deleted base region, or an inserted base region, and/or containing a corresponding wild-type single-stranded DNA (wild-type DNA), and a probe for hybridization with both of the single stranded DNAs, and forming a hybrid (mutant hybrid) with the mutant DNA and/or a hybrid (wild-type hybrid) with the wild-type DNA, the obtained mutant hybrid and/or wild-type hybrid having a stem structure; (2) separating the obtained mutant hybrid and/or wild-type hybrid by electrophoresis according to the presence or absence of the stem structure or differences in the stem structures; and (3) detecting the presence or absence of the mutant DNA in the sample.
Inventors:
MATSUKUMA SHOICHI (JP)
ISHIKAWA TOMOKAZU (JP)
ISHIKAWA TOMOKAZU (JP)
Application Number:
PCT/JP2011/071721
Publication Date:
March 29, 2012
Filing Date:
September 22, 2011
Export Citation:
Assignee:
WAKO PURE CHEM IND LTD (JP)
KANAGAWA PREFECTURAL HOSPITAL ORGANIZATION (JP)
MATSUKUMA SHOICHI (JP)
ISHIKAWA TOMOKAZU (JP)
KANAGAWA PREFECTURAL HOSPITAL ORGANIZATION (JP)
MATSUKUMA SHOICHI (JP)
ISHIKAWA TOMOKAZU (JP)
International Classes:
C12Q1/68; C12N15/09; G01N27/447; G01N33/53
Foreign References:
| JP2007061080A | 2007-03-15 |
Other References:
SHOICHI MATSUKUMA ET AL.: "Loop Hetero Duplex DNA no PAGE deno Kyodo Henka o Riyo shita Idenshi Hen'i Kenshutsu", THE PHYSICO-CHEMICAL BIOLOGY, vol. 52, no. 3, 2008, pages 75, XP008174377
See also references of EP 2620509A4
See also references of EP 2620509A4
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Claims: