Title:
METHOD FOR DETECTING NUCLEIC ACID ON THE BASIS OF DNA NANOROBOT, KIT AND BIOSENSOR
Document Type and Number:
WIPO Patent Application WO/2024/032828
Kind Code:
A2
Abstract:
Disclosed in the present invention are a method for detecting a nucleic acid on the basis of a DNA nanorobot, and the use thereof in a kit or a biosensor. According to the detection method, a padlock probe and a DNAzyme strand are designed according to the sequence of a to-be-detected target gene, and primers, a protection strand and a substrate strand are also correspondingly designed; the amplification of the sequence of a target gene is achieved by means of the NickRCA technique; a DNA nanorobot is constructed by means of DNAzyme; the amplification product binds to the DNA nanorobot to change the conformation of the DNAzyme; the activity of the enzyme is activated to cleave the substrate strand under the action of metal ions to achieve the directional amplification of the signal; a nano-marker is added to the reaction system to serve as a signal reference; and qualitative and/or quantitative analysis of the to-be-detected target gene is achieved by means of sensor detection. The present invention has the advantages of integration, portability, low cost, etc., is suitable for performing on-site rapid and instant detection in an environment with limited conditions, and has great significance for the prevention and control of diseases.
Inventors:
MENG FANMING (CN)
TIAN BO (CN)
LI TINGTING (CN)
TIAN BO (CN)
LI TINGTING (CN)
Application Number:
PCT/CN2023/127710
Publication Date:
February 15, 2024
Filing Date:
October 30, 2023
Export Citation:
Assignee:
UNIV CENTRAL SOUTH (CN)
International Classes:
C12Q1/6844
Attorney, Agent or Firm:
BEIJING DONGFANGSHENGFAN IP AGENCY CO., LTD. (CN)
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