Title:
METHOD FOR DETECTING NUMBER OF COPIES OF TARGET GENE
Document Type and Number:
WIPO Patent Application WO/2023/179053
Kind Code:
A1
Abstract:
The present application provides a method for detecting the number of copies of a target gene. A target gene and an internal reference gene of a sample to be detected are amplified simultaneously in the same reaction; by means of calculating the ratio C (C = ΔRntarget gene / ΔRninternal reference gene) of amplification curve ΔRn values for the target gene and the internal reference gene on qPCR amplification curves, the number of copies of the target gene of the individual to be detected is determined; and according to the range of the ratio, the genotype of the target gene can be effectively detected.
Inventors:
QIAN XUEQING (CN)
QIN WEI (CN)
QIN WEI (CN)
Application Number:
PCT/CN2022/131962
Publication Date:
September 28, 2023
Filing Date:
November 15, 2022
Export Citation:
Assignee:
SHANGHAI RUNDARONGJIA BIOLOGICAL TECH CO LTD (CN)
International Classes:
C12Q1/6858; C12N15/11; C12Q1/6883
Domestic Patent References:
| WO2018144228A1 | 2018-08-09 |
Foreign References:
| CN114457144A | 2022-05-10 | |||
| US20110118145A1 | 2011-05-19 | |||
| CN108048548A | 2018-05-18 | |||
| AU2020104121A4 | 2021-03-04 |
Other References:
TAE-MI LEE ET AL.: "Quantitative Analysis of SMN1 Gene and Estimation of SMN1 Deletion Carrier Frequency in Korean Population based on Real-Time PCR", J KOREAN MED SCI., vol. 19, no. 6, 31 December 2004 (2004-12-31), XP055495151
THOMAS D SCHMITTGEN ET AL.: "Analyzing real-time PCR data by the comparative Ct method", NAT PROTOCOL, vol. 3, no. 6, 5 June 2008 (2008-06-05), XP055137608, DOI: 10.1038/nprot.2008.73
THOMAS D SCHMITTGEN ET AL.: "Analyzing real-time PCR data by the comparative Ct method", NAT PROTOCOL, vol. 3, no. 6, 5 June 2008 (2008-06-05), XP055137608, DOI: 10.1038/nprot.2008.73
Attorney, Agent or Firm:
BEYOND ATTORNEYS AT LAW (CN)
Download PDF: