Title:
METHOD FOR DETECTING PARTICULATE SUBSTANCE USING IMMUNOCHROMATOGRAPHY, AND KIT FOR SAME
Document Type and Number:
WIPO Patent Application WO/2021/045229
Kind Code:
A1
Abstract:
The purpose of the present invention is to provide a highly sensitive method for detecting a particulate substance. The present invention pertains to a method for detecting a particulate substance using immunochromatography, said particulate substance having, on the surface thereof, a plurality of substances to be bound that include a first substance to be bound and a second substance to be bound, which may be the same or different. The method is characterized by comprising: (1) a step in which a sample containing the particulate substance and a first specific binding substance that binds the first substance to be bound are brought into contact on a membrane, and the particulate substance is captured by the first specific binding substance; (2) a step in which the captured particulate substance and a second specific binding substance that binds the second substance to be bound are brought into contact to label the particulate substance; and (3) a step in which the labeled particulate substance is detected. Therein, the first specific binding substance is immobilized on the membrane, and the second specific binding substance is supported on metal nanoparticles.
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Inventors:
MIYAZAWA YUTA (JP)
MIZOGUCHI DAIGOU (JP)
FUJIMOTO HIROTAKA (JP)
WATANABE MAKOTO (JP)
SATO TAKA-AKI (JP)
MIZOGUCHI DAIGOU (JP)
FUJIMOTO HIROTAKA (JP)
WATANABE MAKOTO (JP)
SATO TAKA-AKI (JP)
Application Number:
PCT/JP2020/033823
Publication Date:
March 11, 2021
Filing Date:
September 07, 2020
Export Citation:
Assignee:
DAINIPPON TORYO KK (JP)
SHIMADZU CORP (JP)
SHIMADZU CORP (JP)
International Classes:
G01N33/543; G01N21/78; G01N27/62
Foreign References:
| JP2009002822A | 2009-01-08 | |||
| JP2019007984A | 2019-01-17 | |||
| JP2017106915A | 2017-06-15 | |||
| JP2012112042A | 2012-06-14 | |||
| JP2002267671A | 2002-09-18 | |||
| KR20060109595A | 2006-10-23 | |||
| CN108693347A | 2018-10-23 | |||
| JP2008533472A | 2008-08-21 | |||
| JPS6396559A | 1988-04-27 | |||
| JP2019113425A | 2019-07-11 | |||
| KR20180130640A | 2018-12-10 | |||
| JP2015230280A | 2015-12-21 | |||
| JP2017095744A | 2017-06-01 | |||
| JP2018111014A | 2018-07-19 |
Other References:
JOURNAL OF EXTRACELLULAR VESICLES, vol. 5, no. 1, 2016, pages 31803
BIOSENSORS AND BIOELECTRONICS, vol. 87, 2017, pages 38 - 45
BIOSENSORS AND BIOELECTRONICS, vol. 87, 2017, pages 38 - 45
Attorney, Agent or Firm:
TANAKA Shinichiro et al. (JP)
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