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Title:
METHOD FOR DETECTING PROTEIN CRYSTAL, APPARATUS FOR DETECTING PROTEIN CRYSTAL AND PROGRAM FOR DETECTING PROTEIN CRYSTAL
Document Type and Number:
WIPO Patent Application WO/2004/097366
Kind Code:
A2
Abstract:
It is intended to provide a method for detecting protein crystals, an apparatus for detecting protein crystals and a program for detecting protein crystals by which protein crystals can be efficiently detected at a high reliability. To detect protein crystals, an image obtained by observing a protein solution under crystallization by the vapor diffusion method is differentiated to give a differential image having luminance change data that indicate the extent of luminance changes. After removing noises, the differential image is binarized based on the threshold for extracting a characterizing portion to give a binarized image in which a portion showing a large luminance change remains as the characterizing portion. From this characterizing portion of the binarized image, the presence or absence of protein crystals is judged, thereby judging the occurrence of crystallization. Thus, protein crystal detection, which has been carried out depending exclusively on observation with the naked eye of skilled workers, can be efficiently carried out at a high reliability.

Inventors:
MATSUZAKI HIROFUMI
MIYATAKE HIDEYUKI
KIM SEONG HOON
MIKI KUNIO
Application Number:
PCT/JP2004/006160
Publication Date:
November 11, 2004
Filing Date:
April 28, 2004
Export Citation:
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Assignee:
MATSUSHITA ELECTRIC IND CO LTD (JP)
RIKEN (JP)
MATSUZAKI HIROFUMI
MIYATAKE HIDEYUKI
KIM SEONG HOON
MIKI KUNIO
International Classes:
C30B29/58; B01D9/02; G01N; (IPC1-7): G01N/
Domestic Patent References:
WO1999004361A11999-01-28
Foreign References:
JP2002257730A2002-09-11
Other References:
ZUK W.M. ET AL: 'Methods of analysis of protein crystal images' J. OG CRYSTAL GROWTH vol. 110, 1991, pages 148 - 155, XP000237589
Attorney, Agent or Firm:
IKEUCHI SATO & PARTNER PATENT ATTORNEYS (OAP TOWER 8-30, Tenmabashi 1-chome, Kita-k, Osaka-shi Osaka 26, JP)
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