METHOD OF DETECTING AND QUANTIFYING HEMOLYSIN−PRODUCING BACTERIA BY OVERWHELMINGLY DETECTING AND QUANTIFYING THERMOSTABLE HEMOLYSIN−RELATED GENES &lpar tdh−RELATED HEMOLYSIN GENES&rpar OF FOOD POISONING BACTERIA

WIPO Patent Application WO/2003/033702

A1

Genes of thermostable direct hemolysin proteins [which mean TDH, TRH and hemolytic proteins analogous thereto&semi hereinafter referred to as TDH−related hemolysins] produced by pathogenic bacteria belonging to the genus Vibrio are conveniently and quickly detected, quantified and classified. Namely, a method whereby TDH−related hemolysin−producing bacteria per se, which are important in the management of food sanitation, can be detected and quantified. Using a pair of primers containing a gene sequence encoding an amino acid sequence common to TDH and TRH, TDH−related hemolysin genes are overwhelmingly detected and quantified. Thus, TDH−related hemolysin−producing bacteria per se can be detected and quantified.

KOIZUMI TAKESHI (JP)
YAMAMOTO SATOSHI (JP)
ITOH TAKESHI (JP)
NAKAGAWA HIROSHI (JP)

PCT/JP2002/010795

April 24, 2003

October 17, 2002

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NICHIREI KK (JP)
KOIZUMI TAKESHI (JP)
YAMAMOTO SATOSHI (JP)
ITOH TAKESHI (JP)
NAKAGAWA HIROSHI (JP)

C12N15/09; C12N15/11; C12Q1/02; C12Q1/68; (IPC1-7): C12N15/11; C12Q1/68

JPH04293486A

1992-10-19

BEJ A.K. ET AL.: "Detection of total and hemolysin-producing vibrio parahaemolyticus in shellfish using multiplex PCR amplification to tl, tdh and trh", J. MICROBIOL. METHODS, vol. 36, 1999, pages 215 - 225, XP002909008
See also references of EP 1445313A4

Hiraki, Yusuke (17-1 Toranomon 1-chom, Minato-ku Tokyo, JP)

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