Title:
METHOD FOR DETECTING TARGET GENE, USING DCAS9/GRNA COMPLEX AND FLUORESCENCE MARKER
Document Type and Number:
WIPO Patent Application WO/2018/151339
Kind Code:
A1
Abstract:
The present invention relates to a method for detecting a target gene, the method comprising: a first step of reacting a complex composed of deactivated Cas9 (dCas9) and a guide RNA specifically binding to a target gene with a sample including a target gene; a second step of separating the reactants; and a third step of treating with a fluorescence marker. A method for detecting a target gene according to the present invention allows the detection of a target gene at high sensitivity even without performing PCR and can be useful for detecting a target gene rapidly and accurately, without a separate gene isolation step.
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Inventors:
JUNG JUYEON (KR)
LIM EUN KYUNG (KR)
GUK KYEONG HYE (KR)
KEEM JOO OAK (KR)
KANG TAEJOON (KR)
LIM EUN KYUNG (KR)
GUK KYEONG HYE (KR)
KEEM JOO OAK (KR)
KANG TAEJOON (KR)
Application Number:
PCT/KR2017/001618
Publication Date:
August 23, 2018
Filing Date:
February 14, 2017
Export Citation:
Assignee:
KOREA RES INST BIOSCIENCE & BIOTECHNOLOGY (KR)
International Classes:
C12Q1/68; C12N9/16; C12N15/11
Foreign References:
| US20140356867A1 | 2014-12-04 | |||
| KR20160050069A | 2016-05-10 | |||
| KR20160052400A | 2016-05-12 | |||
| KR20060128218A | 2006-12-14 | |||
| KR100832738B1 | 2008-05-27 |
Attorney, Agent or Firm:
AHN, So Young (KR)
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