METHOD FOR DETECTING TARGET MOLECULE, AND KIT FOR USE IN SAID METHOD

WIPO Patent Application WO/2016/047068

A1

Provided is a method for detecting a target molecule, which comprises: a complex formation procedure of reacting the target molecule with a carrier which is modified with a first antibody capable of binding to the target molecule specifically and at least two second antibodies which are capable of binding to the target molecule specifically and are respectively labeled with enzymes each having a substrate-cleaving activity and in which the substrate specificities of the enzymes are different from each other, thereby forming a complex composed of the first antibody, the target molecule and the second antibodies all provided on the carrier; and a detection procedure of reacting at least two substrates which respectively have cleavage sites for the enzymes and in each of which a fluorescent substance is bound to one end side of the cleavage site and a color-fading substance is bound to the other end side and in which the fluorescence wavelengths of the fluorescent substances are different from each other with the complex, and then detecting fluorescence emitted from each of the fluorescent substances. It becomes possible to remove noises resulted from the non-specific adsorption of the detection antibodies onto the carrier and to detect a signal coming from the target molecule with high sensitivity and high accuracy.

NOJI HIROYUKI (JP)

PCT/JP2015/004577

March 31, 2016

September 09, 2015

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JAPAN SCIENCE & TECH AGENCY (JP)

G01N33/543; C12Q1/68

JP2009506312A	2009-02-12
JP2009000673A	2009-01-08
JP2007525661A	2007-09-06
US20140228239A1	2014-08-14
US20090176259A1	2009-07-09

See also references of EP 3199948A4

OHNO, Seiji et al. (JP)
Seiji Ono (JP)

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